UniProtKB - Q9LVN7 (DPOD1_ARATH)
DNA polymerase delta catalytic subunit
POLD1
Functioni
This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction.
Miscellaneous
Catalytic activityi
- EC:2.7.7.7
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1007 | ZincBy similarity | 1 | |
Metal bindingi | 1010 | ZincBy similarity | 1 | |
Metal bindingi | 1020 | ZincBy similarity | 1 | |
Metal bindingi | 1023 | ZincBy similarity | 1 | |
Metal bindingi | 1052 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 1055 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 1065 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 1070 | Iron-sulfur (4Fe-4S)By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1007 – 1023 | CysA-typeAdd BLAST | 17 |
GO - Molecular functioni
- 3'-5'-exodeoxyribonuclease activity Source: GO_Central
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- nucleotide binding Source: InterPro
GO - Biological processi
- base-excision repair, gap-filling Source: GO_Central
- DNA-dependent DNA replication Source: GO_Central
- DNA replication proofreading Source: GO_Central
- nucleotide-excision repair, DNA gap filling Source: GO_Central
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase delta catalytic subunit (EC:2.7.7.7)Alternative name(s): Protein EMBRYO DEFECTIVE 2780 |
Gene namesi | Name:POLD1 Synonyms:EMB2780 Ordered Locus Names:At5g63960 ORF Names:MBM17.6 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G63960 |
Subcellular locationi
Nucleus
Nucleus
- delta DNA polymerase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046449 | 1 – 1095 | DNA polymerase delta catalytic subunitAdd BLAST | 1095 |
Proteomic databases
PaxDbi | Q9LVN7 |
PRIDEi | Q9LVN7 |
ProteomicsDBi | 241250 [Q9LVN7-1] |
Interactioni
Subunit structurei
Heterodimer with subunits of 125 kDa and 50 kDa. The 125 kDa subunit contains the polymerase active site and most likely the active site for the 3'-5' exonuclease activity (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 21759, 4 interactors |
IntActi | Q9LVN7, 2 interactors |
STRINGi | 3702.AT5G63960.2 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 37 | DisorderedSequence analysisAdd BLAST | 37 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1052 – 1070 | CysB motifAdd BLAST | 19 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1007 – 1023 | CysA-typeAdd BLAST | 17 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0969, Eukaryota |
InParanoidi | Q9LVN7 |
OMAi | GNQKSPY |
PhylomeDBi | Q9LVN7 |
Family and domain databases
Gene3Di | 1.10.132.60, 1 hit 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR042087, DNA_pol_B_thumb IPR023211, DNA_pol_palm_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf IPR025687, Znf-C4pol |
Pfami | View protein in Pfam PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 1 hit PF14260, zf-C4pol, 1 hit |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketThis entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNRSGISKKR PPPSNTPPPA GKHRATGDST PSPAIGTLDD EFMMEEDVFL
60 70 80 90 100
DETLLYGDED EESLILRDIE ERESRSSAWA RPPLSPAYLS NSQSIIFQQL
110 120 130 140 150
EIDSIIAESH KELLPGSSGQ APIIRMFGVT REGNSVCCFV HGFEPYFYIA
160 170 180 190 200
CPPGMGPDDI SNFHQSLEGR MRESNKNAKV PKFVKRIEMV QKRSIMYYQQ
210 220 230 240 250
QKSQTFLKIT VALPTMVASC RGILDRGLQI DGLGMKSFQT YESNILFVLR
260 270 280 290 300
FMVDCDIVGG NWIEVPTGKY KKNARTLSYC QLEFHCLYSD LISHAAEGEY
310 320 330 340 350
SKMAPFRVLS FDIECAGRKG HFPEAKHDPV IQIANLVTLQ GEDHPFVRNV
360 370 380 390 400
MTLKSCAPIV GVDVMSFETE REVLLAWRDL IRDVDPDIII GYNICKFDLP
410 420 430 440 450
YLIERAATLG IEEFPLLGRV KNSRVRVRDS TFSSRQQGIR ESKETTIEGR
460 470 480 490 500
FQFDLIQAIH RDHKLSSYSL NSVSAHFLSE QKEDVHHSII TDLQNGNAET
510 520 530 540 550
RRRLAVYCLK DAYLPQRLLD KLMFIYNYVE MARVTGVPIS FLLARGQSIK
560 570 580 590 600
VLSQLLRKGK QKNLVLPNAK QSGSEQGTYE GATVLEARTG FYEKPIATLD
610 620 630 640 650
FASLYPSIMM AYNLCYCTLV TPEDVRKLNL PPEHVTKTPS GETFVKQTLQ
660 670 680 690 700
KGILPEILEE LLTARKRAKA DLKEAKDPLE KAVLDGRQLA LKISANSVYG
710 720 730 740 750
FTGATVGQLP CLEISSSVTS YGRQMIEQTK KLVEDKFTTL GGYQYNAEVI
760 770 780 790 800
YGDTDSVMVQ FGVSDVEAAM TLGREAAEHI SGTFIKPIKL EFEKVYFPYL
810 820 830 840 850
LINKKRYAGL LWTNPQQFDK MDTKGIETVR RDNCLLVKNL VTESLNKILI
860 870 880 890 900
DRDVPGAAEN VKKTISDLLM NRIDLSLLVI TKGLTKTGDD YEVKSAHGEL
910 920 930 940 950
AERMRKRDAA TAPNVGDRVP YVIIKAAKGA KAYERSEDPI YVLQNNIPID
960 970 980 990 1000
PNYYLENQIS KPLLRIFEPV LKNASKELLH GSHTRSISIT TPSNSGIMKF
1010 1020 1030 1040 1050
AKKQLSCVGC KVPISNGTLC ASCKGREAEL YCKNVSQVAE LEEVFGRLWT
1060 1070 1080 1090
QCQECQGSLH QDVLCTSRDC PIFYRRMKAQ KDMAVARQQL DRWSF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4KC69 | F4KC69_ARATH | DNA polymerase | EMBRYO DEFECTIVE 2780 MBM17.6, MBM17_6, At5g63960 | 1,112 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB019227 Genomic DNA Translation: BAA96899.1 Sequence problems. CP002688 Genomic DNA Translation: AED97821.1 DQ160246 mRNA Translation: ABA41487.1 |
RefSeqi | NP_201201.2, NM_125792.2 [Q9LVN7-1] |
Genome annotation databases
EnsemblPlantsi | AT5G63960.1; AT5G63960.1; AT5G63960 [Q9LVN7-1] |
GeneIDi | 836517 |
Gramenei | AT5G63960.1; AT5G63960.1; AT5G63960 [Q9LVN7-1] |
KEGGi | ath:AT5G63960 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB019227 Genomic DNA Translation: BAA96899.1 Sequence problems. CP002688 Genomic DNA Translation: AED97821.1 DQ160246 mRNA Translation: ABA41487.1 |
RefSeqi | NP_201201.2, NM_125792.2 [Q9LVN7-1] |
3D structure databases
AlphaFoldDBi | Q9LVN7 |
SMRi | Q9LVN7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 21759, 4 interactors |
IntActi | Q9LVN7, 2 interactors |
STRINGi | 3702.AT5G63960.2 |
Proteomic databases
PaxDbi | Q9LVN7 |
PRIDEi | Q9LVN7 |
ProteomicsDBi | 241250 [Q9LVN7-1] |
Genome annotation databases
EnsemblPlantsi | AT5G63960.1; AT5G63960.1; AT5G63960 [Q9LVN7-1] |
GeneIDi | 836517 |
Gramenei | AT5G63960.1; AT5G63960.1; AT5G63960 [Q9LVN7-1] |
KEGGi | ath:AT5G63960 |
Organism-specific databases
Araporti | AT5G63960 |
Phylogenomic databases
eggNOGi | KOG0969, Eukaryota |
InParanoidi | Q9LVN7 |
OMAi | GNQKSPY |
PhylomeDBi | Q9LVN7 |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 399 |
Miscellaneous databases
PROi | PR:Q9LVN7 |
Gene expression databases
ExpressionAtlasi | Q9LVN7, baseline and differential |
Family and domain databases
Gene3Di | 1.10.132.60, 1 hit 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR042087, DNA_pol_B_thumb IPR023211, DNA_pol_palm_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf IPR025687, Znf-C4pol |
Pfami | View protein in Pfam PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 1 hit PF14260, zf-C4pol, 1 hit |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOD1_ARATH | |
Accessioni | Q9LVN7Primary (citable) accession number: Q9LVN7 Secondary accession number(s): A0MA44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | February 8, 2011 | |
Last modified: | May 25, 2022 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families