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UniProtKB - Q9LVK9 (ALA7_ARATH)
Protein
Probable phospholipid-transporting ATPase 7
Gene
ALA7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Involved in transport of phospholipids and in regulation of pollen plasma membrane lipid asymmetry.
1 PublicationCatalytic activityi
- ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).1 Publication EC:7.6.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 425 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 886 | MagnesiumBy similarity | 1 | |
Metal bindingi | 890 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase-coupled intramembrane lipid transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- magnesium ion binding Source: InterPro
GO - Biological processi
- phospholipid translocation Source: GO_Central
- pollen tube growth Source: TAIR
- regulation of membrane lipid metabolic process Source: TAIR
Keywordsi
Molecular function | Translocase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT3G13900-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Probable phospholipid-transporting ATPase 71 Publication (EC:7.6.2.11 Publication)Short name: AtALA71 Publication Alternative name(s): Aminophospholipid flippase 71 Publication |
Gene namesi | Name:ALA71 Publication Ordered Locus Names:At3g13900Imported ORF Names:MDC16.2Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G13900 |
TAIRi | locus:2088217, AT3G13900 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Other locations
- Endomembrane system By similarity; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- endomembrane system Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 74 | CytoplasmicSequence analysisAdd BLAST | 74 | |
Transmembranei | 75 – 96 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 97 – 100 | ExtracellularSequence analysis | 4 | |
Transmembranei | 101 – 123 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 124 – 305 | CytoplasmicSequence analysisAdd BLAST | 182 | |
Transmembranei | 306 – 327 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 328 – 359 | ExtracellularSequence analysisAdd BLAST | 32 | |
Transmembranei | 360 – 377 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 378 – 941 | CytoplasmicSequence analysisAdd BLAST | 564 | |
Transmembranei | 942 – 961 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 962 – 975 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 976 – 995 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 996 – 1025 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 1026 – 1048 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 1049 – 1061 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 1062 – 1084 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 1085 – 1090 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 1091 – 1111 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1112 – 1128 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 1129 – 1153 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 1154 – 1243 | CytoplasmicSequence analysisAdd BLAST | 90 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Ala6 and ala7 double mutants are hypersensitive to temperature stress and are impaired in pollen fitness with an altered lipid composition and short and slow pollen tubes.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046391 | 1 – 1243 | Probable phospholipid-transporting ATPase 7Add BLAST | 1243 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 623 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q9LVK9 |
ProteomicsDBi | 245016 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9LVK9, baseline and differential |
Genevisiblei | Q9LVK9, AT |
Family & Domainsi
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0206, Eukaryota |
HOGENOMi | CLU_000846_3_1_1 |
InParanoidi | Q9LVK9 |
OMAi | NKHKFFI |
OrthoDBi | 587717at2759 |
Family and domain databases
Gene3Di | 3.40.1110.10, 2 hits 3.40.50.1000, 2 hits |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9LVK9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGRRRIRSRI RKSHFYTFKC LRPKTLEDQG PHIINGPGYT RIVHCNQPHL
60 70 80 90 100
HLAKVLRYTS NYVSTTRYNL ITFLPKCLYE QFHRVANFYF LVAAILSVFP
110 120 130 140 150
LSPFNKWSMI APLIFVVGLS MGKEALEDWR RFMQDVKVNS RKATVHRGDG
160 170 180 190 200
DFGRRKWKKL RVGDVVKVEK DQFFPADLLL LSSSYEDGIC YVETMNLDGE
210 220 230 240 250
TNLKVKRCLD VTLPLERDDT FQSFSGTIKC EDPNPNLYTF VGNLEYDGQV
260 270 280 290 300
YPLDPSQILL RDSKLRNTSY VYGVVVFTGH DTKVMQNSTK SPSKRSRIEK
310 320 330 340 350
RMDYIIYTLF ALLVLVSFIS SLGFAVMTKM HMGDWWYLRP DKPERLTNPR
360 370 380 390 400
NPFHAWVVHL ITAVLLYGYL IPISLYVSIE LVKVLQATFI NQDLQMYDSE
410 420 430 440 450
SGTPAQARTS NLNEELGQVD TILSDKTGTL TCNQMDFLKC SIAGTSYGVR
460 470 480 490 500
ASEVELAAAK QMAIDLDEEQ GEEVTHLPRT RGRMHGYAKM PSKTSSDIEL
510 520 530 540 550
ETVITATDEG DQTQSTGIKG FSFEDQRLMG GNWLNEPNSD DILMFLRILA
560 570 580 590 600
VCHTAIPEVD EDTGKCTYEA ESPDEVAFLV AAGEFGFEFT KRTQSSVFIS
610 620 630 640 650
ERHSGQPVER EYKVLNVLDF TSKRKRMSVI VRDEKGQILL LCKGADSIIF
660 670 680 690 700
ERLSKNGKNY LEATSKHLNG YGEAGLRTLA LSYRKLDETE YSIWNSEFHK
710 720 730 740 750
AKTSVGADRD EMLEKVSDMM EKELILVGAT AVEDKLQKGV PQCIDKLAQA
760 770 780 790 800
GLKIWVLTGD KMETAINIGY ACSLLRQGMK QIYIALRNEE GSSQDPEAAA
810 820 830 840 850
RENILMQIIN ASQMIKLEKD PHAAFALIID GKTLTYALED DIKYQFLALA
860 870 880 890 900
VDCASVICCR VSPKQKALVT RLAKEGTGKT TLAIGDGAND VGMIQEADIG
910 920 930 940 950
VGISGVEGMQ AVMASDFSIA QFRFLERLLV VHGHWCYKRI AQMICYFFYK
960 970 980 990 1000
NITFGLTLFY FEAFTGFSGQ AIYNDSYLLL FNVILTSLPV IALGVFEQDV
1010 1020 1030 1040 1050
SSEVCLQFPA LYQQGPKNLF FDWYRIIGWM ANGVYASVVI FSLNIGIFHV
1060 1070 1080 1090 1100
QSFCSGGQTA DMDAMGTAMF TCIIWAVNVQ IALTMSHFTW IQHVLIWGSI
1110 1120 1130 1140 1150
VTWYIFLALF GMLPPKVSGN IFHMLSETLA PAPIFWLTSL LVIAATTLPY
1160 1170 1180 1190 1200
LAYISFQRSL NPLDHHIIQE IKHFRIDVQD ECMWTRERSK AREKTKIGVT
1210 1220 1230 1240
ARVDAKIRQL RGRLQRKHSI LSVMSGLSGV SASTDTTSTT QHS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1I9LT85 | A0A1I9LT85_ARATH | Phospholipid-transporting ATPase | At3g13900 | 1,009 | Annotation score: | ||
A0A1I9LT86 | A0A1I9LT86_ARATH | Phospholipid-transporting ATPase | At3g13900 | 966 | Annotation score: |
Sequence cautioni
The sequence BAB02320 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 614 | V → F in BAB02320 (PubMed:10819329).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB019229 Genomic DNA Translation: BAB02320.1 Sequence problems. CP002686 Genomic DNA Translation: AEE75436.1 |
RefSeqi | NP_188006.4, NM_112244.5 |
Genome annotation databases
EnsemblPlantsi | AT3G13900.1; AT3G13900.1; AT3G13900 |
GeneIDi | 820603 |
Gramenei | AT3G13900.1; AT3G13900.1; AT3G13900 |
KEGGi | ath:AT3G13900 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB019229 Genomic DNA Translation: BAB02320.1 Sequence problems. CP002686 Genomic DNA Translation: AEE75436.1 |
RefSeqi | NP_188006.4, NM_112244.5 |
3D structure databases
AlphaFoldDBi | Q9LVK9 |
SMRi | Q9LVK9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT3G13900.1 |
Proteomic databases
PaxDbi | Q9LVK9 |
ProteomicsDBi | 245016 |
Genome annotation databases
EnsemblPlantsi | AT3G13900.1; AT3G13900.1; AT3G13900 |
GeneIDi | 820603 |
Gramenei | AT3G13900.1; AT3G13900.1; AT3G13900 |
KEGGi | ath:AT3G13900 |
Organism-specific databases
Araporti | AT3G13900 |
TAIRi | locus:2088217, AT3G13900 |
Phylogenomic databases
eggNOGi | KOG0206, Eukaryota |
HOGENOMi | CLU_000846_3_1_1 |
InParanoidi | Q9LVK9 |
OMAi | NKHKFFI |
OrthoDBi | 587717at2759 |
Enzyme and pathway databases
BioCyci | ARA:AT3G13900-MONOMER |
Miscellaneous databases
PROi | PR:Q9LVK9 |
Gene expression databases
ExpressionAtlasi | Q9LVK9, baseline and differential |
Genevisiblei | Q9LVK9, AT |
Family and domain databases
Gene3Di | 3.40.1110.10, 2 hits 3.40.50.1000, 2 hits |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALA7_ARATH | |
Accessioni | Q9LVK9Primary (citable) accession number: Q9LVK9 Secondary accession number(s): F4JEL2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | January 25, 2012 | |
Last modified: | May 25, 2022 | |
This is version 157 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families