ACA9

Functionⁱ

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.By similarity

Catalytic activityⁱ

ATP + H₂O + Ca²⁺(Side 1) = ADP + phosphate + Ca²⁺(Side 2).

Activity regulationⁱ

Activated by calmodulin.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	495	4-aspartylphosphate intermediateBy similarity	1
Metal bindingⁱ	802	MagnesiumBy similarity	1
Metal bindingⁱ	806	MagnesiumBy similarity	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
calcium-transporting ATPase activity
Source: TAIR
Inferred from direct assayⁱ
- 15197266
calmodulin binding
Source: TAIR
Inferred from direct assayⁱ
- 15197266
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

pollen development
Source: TAIR
Inferred from mutant phenotypeⁱ
- 15197266
single fertilization
Source: TAIR
Inferred from mutant phenotypeⁱ
- 15197266

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Calmodulin-binding, Hydrolase
Biological process	Calcium transport, Ion transport, Transport
Ligand	ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	ARA:AT3G21180-MONOMER
Reactomeⁱ	R-ATH-418359 Reduction of cytosolic Ca++ levels R-ATH-5578775 Ion homeostasis R-ATH-936837 Ion transport by P-type ATPases

Protein family/group databases

TCDBⁱ	3.A.3.2.14 the p-type atpase (p-atpase) superfamily

TCDBⁱ

3.A.3.2.14 the p-type atpase (p-atpase) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Calcium-transporting ATPase 9, plasma membrane-type (EC:3.6.3.8) Alternative name(s): Ca(2+)-ATPase isoform 9
Gene namesⁱ	Name:ACA9 Ordered Locus Names:At3g21180 ORF Names:MXL8.3
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 3

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT3G21180
TAIRⁱ	locus:2094726 AT3G21180

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 194	CytoplasmicSequence analysisAdd BLAST	194
Transmembraneⁱ	195 – 215	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	216 – 233	LumenalSequence analysisAdd BLAST	18
Transmembraneⁱ	234 – 254	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	255 – 382	CytoplasmicSequence analysisAdd BLAST	128
Transmembraneⁱ	383 – 402	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	403 – 439	LumenalSequence analysisAdd BLAST	37
Transmembraneⁱ	440 – 457	HelicalSequence analysisAdd BLAST	18
Topological domainⁱ	458 – 857	CytoplasmicSequence analysisAdd BLAST	400
Transmembraneⁱ	858 – 876	HelicalSequence analysisAdd BLAST	19
Topological domainⁱ	877 – 887	LumenalSequence analysisAdd BLAST	11
Transmembraneⁱ	888 – 908	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	909 – 928	CytoplasmicSequence analysisAdd BLAST	20
Transmembraneⁱ	929 – 951	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	952 – 963	LumenalSequence analysisAdd BLAST	12
Transmembraneⁱ	964 – 988	HelicalSequence analysisAdd BLAST	25
Topological domainⁱ	989 – 1006	CytoplasmicSequence analysisAdd BLAST	18
Transmembraneⁱ	1007 – 1028	HelicalSequence analysisAdd BLAST	22
Topological domainⁱ	1029 – 1038	LumenalSequence analysis	10
Transmembraneⁱ	1039 – 1060	HelicalSequence analysisAdd BLAST	22
Topological domainⁱ	1061 – 1086	CytoplasmicSequence analysisAdd BLAST	26

Keywords - Cellular componentⁱ

Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000046415	1 – 1086	Calcium-transporting ATPase 9, plasma membrane-typeAdd BLAST		1086

Proteomic databases

PaxDbⁱ	Q9LU41
PRIDEⁱ	Q9LU41

PTM databases

iPTMnetⁱ	Q9LU41

iPTMnetⁱ

Q9LU41

Expressionⁱ

Gene expression databases

ExpressionAtlasⁱ	Q9LU41 baseline and differential
Genevisibleⁱ	Q9LU41 AT

Interactionⁱ

GO - Molecular functionⁱ

calmodulin binding
Source: TAIR
Inferred from direct assayⁱ
- 15197266

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	3702.AT3G21180.1

STRINGⁱ

3702.AT3G21180.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9LU41
SMRⁱ	Q9LU41
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	57 – 68	Interaction with calmodulinBy similarityAdd BLAST		12

Domainⁱ

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity

Sequence similaritiesⁱ

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0204 Eukaryota ENOG410XNNC LUCA
HOGENOMⁱ	HOG000265623
InParanoidⁱ	Q9LU41
KEGG Orthology (KO) More...KOⁱ	K01537
OMAⁱ	AIIHAFT
Database of Orthologous Groups More...OrthoDBⁱ	EOG093601NF
PhylomeDBⁱ	Q9LU41

Family and domain databases

Gene3Dⁱ	3.40.1110.10, 1 hit 3.40.50.1000, 1 hit
InterProⁱ	View protein in InterPro IPR006068 ATPase_P-typ_cation-transptr_C IPR004014 ATPase_P-typ_cation-transptr_N IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR024750 Ca_ATPase_N_dom IPR036412 HAD-like_sf IPR023214 HAD_sf IPR006408 P-type_ATPase_IIB IPR001757 P_typ_ATPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12515 CaATP_NAI, 1 hit PF00689 Cation_ATPase_C, 1 hit PF00690 Cation_ATPase_N, 1 hit
SMARTⁱ	View protein in SMART SM00831 Cation_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 1 hit SSF81653 SSF81653, 1 hit SSF81660 SSF81660, 1 hit SSF81665 SSF81665, 1 hit
TIGRFAMsⁱ	TIGR01517 ATPase-IIB_Ca, 1 hit TIGR01494 ATPase_P-type, 3 hits
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9LU41-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSTSSSNGLL LTSMSGRHDD MEAGSAKTEE HSDHEELQHD PDDPFDIDNT 
        60         70         80         90        100
KNASVESLRR WRQAALVLNA SRRFRYTLDL NKEEHYDNRR RMIRAHAQVI 
       110        120        130        140        150
RAALLFKLAG EQQIAFGSST PAASTGNFDI DLEKLVSMTR NQNMSNLQQY 
       160        170        180        190        200
GGVKGVAEKL KSNMEQGINE DEKEVIDRKN AFGSNTYPKK KGKNFFMFLW 
       210        220        230        240        250
EAWQDLTLII LIIAAVTSLA LGIKTEGLKE GWLDGGSIAF AVLLVIVVTA 
       260        270        280        290        300
VSDYRQSLQF QNLNDEKRNI QLEVMRGGRT VKISIYDVVV GDVIPLRIGD 
       310        320        330        340        350
QVPADGVLIS GHSLAIDESS MTGESKIVHK DQKSPFLMSG CKVADGVGNM 
       360        370        380        390        400
LVTGVGINTE WGLLMASISE DTGEETPLQV RLNGLATFIG IVGLSVALVV 
       410        420        430        440        450
LVALLVRYFT GTTQDTNGAT QFIKGTTSIS DIVDDCVKIF TIAVTIVVVA 
       460        470        480        490        500
VPEGLPLAVT LTLAYSMRKM MADKALVRRL SACETMGSAT TICSDKTGTL 
       510        520        530        540        550
TLNQMTVVET YAGGSKMDVA DNPSGLHPKL VALISEGVAQ NTTGNIFHPK 
       560        570        580        590        600
DGGEVEISGS PTEKAILSWA YKLGMKFDTI RSESAIIHAF PFNSEKKRGG 
       610        620        630        640        650
VAVLRGDSEV FIHWKGAAEI VLACCTQYMD SNGTLQSIES QKEFFRVAID 
       660        670        680        690        700
SMAKNSLRCV AIACRTQELN QVPKEQEDLD KWALPEDELI LLAIVGIKDP 
       710        720        730        740        750
CRPGVREAVR ICTSAGVKVR MVTGDNLQTA KAIALECGIL SSDTEAVEPT 
       760        770        780        790        800
IIEGKVFREL SEKEREQVAK KITVMGRSSP NDKLLLVQAL RKNGDVVAVT 
       810        820        830        840        850
GDGTNDAPAL HEADIGLSMG ISGTEVAKES SDIIILDDNF ASVVKVVRWG 
       860        870        880        890        900
RSVYANIQKF IQFQLTVNVA ALIINVVAAM SSGDVPLKAV QLLWVNLIMD 
       910        920        930        940        950
TLGALALATE PPTDHLMHRT PVGRREPLIT NIMWRNLLVQ SFYQVAVLLV 
       960        970        980        990       1000
LNFAGLSILG LNHENHAHAV EVKNTMIFNA FVMCQIFNEF NARKPDEMNV 
      1010       1020       1030       1040       1050
FRGVNKNPLF VAIVGVTFIL QIIIVTFLGK FAHTVRLGWQ LWLASIIIGL 
      1060       1070       1080 
VSWPLAIVGK LIPVPKTPMS VYFKKPFRKY KASRNA

Length:1,086

Mass (Da):118,768

Last modified:June 26, 2007 - v2

Checksum:ⁱEC317264EE1C3098

GO

Sequence cautionⁱ

The sequence BAB01709 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB023045 Genomic DNA Translation: BAB01709.1 Different initiation. CP002686 Genomic DNA Translation: AEE76473.1 AK227228 mRNA Translation: BAE99265.1
NCBI Reference Sequences More...RefSeqⁱ	NP_188755.2, NM_113013.5
UniGeneⁱ	At.38102

Genome annotation databases

EnsemblPlantsⁱ	AT3G21180.1; AT3G21180.1; AT3G21180
GeneIDⁱ	821671
Grameneⁱ	AT3G21180.1; AT3G21180.1; AT3G21180
KEGGⁱ	ath:AT3G21180

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9LU41	Calcium-transporting ATPase		ARATH		1086	UniRef100_Q9LU41

Protein	Similar proteins		Species	Score	Length	Source
Q9LU41	Calcium-transporting ATPase		ARATH		1086	UniRef90_Q9LU41
Calcium-transporting ATPase		BRARP		1096
Calcium-transporting ATPase		BRANA		602
Calcium-transporting ATPase		Brassica oleracea var. oleracea		1095
Calcium-transporting ATPase		Capsella rubella		1088
+28

Protein	Similar proteins		Species	Score	Length	Source
Q9LU41	Calcium-transporting ATPase		ARATH		1086	UniRef50_Q9LU41
Calcium-transporting ATPase		BRARP		1096
Calcium-transporting ATPase		Brassica oleracea var. oleracea		1095
Calcium-transporting ATPase		BRANA		1095
Calcium-transporting ATPase		Capsella rubella		1088
+127

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB023045 Genomic DNA Translation: BAB01709.1 Different initiation. CP002686 Genomic DNA Translation: AEE76473.1 AK227228 mRNA Translation: BAE99265.1
RefSeqⁱ	NP_188755.2, NM_113013.5
UniGeneⁱ	At.38102

3D structure databases

ProteinModelPortalⁱ	Q9LU41
SMRⁱ	Q9LU41
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	3702.AT3G21180.1

STRINGⁱ

3702.AT3G21180.1

Protein family/group databases

TCDBⁱ	3.A.3.2.14 the p-type atpase (p-atpase) superfamily

TCDBⁱ

3.A.3.2.14 the p-type atpase (p-atpase) superfamily

PTM databases

iPTMnetⁱ	Q9LU41

iPTMnetⁱ

Q9LU41

Proteomic databases

PaxDbⁱ	Q9LU41
PRIDEⁱ	Q9LU41

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT3G21180.1; AT3G21180.1; AT3G21180
GeneIDⁱ	821671
Grameneⁱ	AT3G21180.1; AT3G21180.1; AT3G21180
KEGGⁱ	ath:AT3G21180

Organism-specific databases

Araportⁱ	AT3G21180
TAIRⁱ	locus:2094726 AT3G21180

Phylogenomic databases

eggNOGⁱ	KOG0204 Eukaryota ENOG410XNNC LUCA
HOGENOMⁱ	HOG000265623
InParanoidⁱ	Q9LU41
KOⁱ	K01537
OMAⁱ	AIIHAFT
OrthoDBⁱ	EOG093601NF
PhylomeDBⁱ	Q9LU41

Enzyme and pathway databases

BioCycⁱ	ARA:AT3G21180-MONOMER
Reactomeⁱ	R-ATH-418359 Reduction of cytosolic Ca++ levels R-ATH-5578775 Ion homeostasis R-ATH-936837 Ion transport by P-type ATPases

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9LU41

PROⁱ

PR:Q9LU41

Gene expression databases

ExpressionAtlasⁱ	Q9LU41 baseline and differential
Genevisibleⁱ	Q9LU41 AT

Family and domain databases

Gene3Dⁱ	3.40.1110.10, 1 hit 3.40.50.1000, 1 hit
InterProⁱ	View protein in InterPro IPR006068 ATPase_P-typ_cation-transptr_C IPR004014 ATPase_P-typ_cation-transptr_N IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR024750 Ca_ATPase_N_dom IPR036412 HAD-like_sf IPR023214 HAD_sf IPR006408 P-type_ATPase_IIB IPR001757 P_typ_ATPase
Pfamⁱ	View protein in Pfam PF12515 CaATP_NAI, 1 hit PF00689 Cation_ATPase_C, 1 hit PF00690 Cation_ATPase_N, 1 hit
SMARTⁱ	View protein in SMART SM00831 Cation_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 1 hit SSF81653 SSF81653, 1 hit SSF81660 SSF81660, 1 hit SSF81665 SSF81665, 1 hit
TIGRFAMsⁱ	TIGR01517 ATPase-IIB_Ca, 1 hit TIGR01494 ATPase_P-type, 3 hits
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ACA9_ARATH
Accessionⁱ	Q9LU41Primary (citable) accession number: Q9LU41 Secondary accession number(s): Q0WUD3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
	Last sequence update:	June 26, 2007
	Last modified:	November 7, 2018
	This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

UniProtKB

UniParc

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Supporting data

UniProtKB - Q9LU41 (ACA9_ARATH)

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Calcium-transporting ATPase 9, plasma membrane-type

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Plasma Membrane

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ