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UniProtKB - Q9LTS3 (CKX3_ARATH)
Protein
Cytokinin dehydrogenase 3
Gene
CKX3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group.
1 PublicationCatalytic activityi
- EC:1.5.99.12
Cofactori
FADBy similarity
Kineticsi
- KM=7.2 µM for isopentenyladenine1 Publication
- Vmax=7.7 nmol/h/mg enzyme1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 110 | FAD; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 167 | FAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 172 | FADBy similarity | 1 | |
Binding sitei | 233 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 476 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 100 – 104 | FADBy similarity | 5 | |
Nucleotide bindingi | 105 – 106 | FADBy similarity | 2 | |
Nucleotide bindingi | 178 – 182 | FADBy similarity | 5 | |
Nucleotide bindingi | 511 – 514 | FADBy similarity | 4 |
GO - Molecular functioni
- cytokinin dehydrogenase activity Source: TAIR
- FAD binding Source: InterPro
- oxidoreductase activity Source: GO_Central
- primary amine oxidase activity Source: TAIR
GO - Biological processi
- cytokinin catabolic process Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | ARA:AT5G56970-MONOMER |
BRENDAi | 1.5.99.12, 399 |
SABIO-RKi | Q9LTS3 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytokinin dehydrogenase 3 (EC:1.5.99.12)Alternative name(s): Cytokinin oxidase 3 Short name: AtCKX3 Short name: CKO 3 |
Gene namesi | Name:CKX3 Ordered Locus Names:At5g56970 ORF Names:MHM17.8 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G56970 |
TAIRi | locus:2164615, AT5G56970 |
Subcellular locationi
Vacuole
- Vacuole 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Vacuole
- vacuole Source: TAIR
Keywords - Cellular componenti
Endoplasmic reticulum, VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Sequence analysisAdd BLAST | 31 | |
ChainiPRO_0000020420 | 32 – 523 | Cytokinin dehydrogenase 3Add BLAST | 492 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 105 | Pros-8alpha-FAD histidineBy similarity | 1 | |
Glycosylationi | 153 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 408 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q9LTS3 |
ProteomicsDBi | 222614 |
PTM databases
iPTMneti | Q9LTS3 |
Expressioni
Tissue specificityi
Very weak expression in the young shoot tissues around two weeks after germination (PubMed:14555694). Present in the center of the floral meristem and the boundary between long stamen primordia and gynoecial primordia (PubMed:26390296).2 Publications
Inductioni
Regulated by GATA18/HAN.1 Publication
Gene expression databases
ExpressionAtlasi | Q9LTS3, baseline and differential |
Genevisiblei | Q9LTS3, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 21043, 2 interactors |
STRINGi | 3702.AT5G56970.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 66 – 243 | FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST | 178 |
Sequence similaritiesi
Belongs to the oxygen-dependent FAD-linked oxidoreductase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1231, Eukaryota |
HOGENOMi | CLU_024955_1_0_1 |
InParanoidi | Q9LTS3 |
OrthoDBi | 350817at2759 |
PhylomeDBi | Q9LTS3 |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.40.462.10, 1 hit |
InterProi | View protein in InterPro IPR016170, Cytok_DH_C_sf IPR015345, Cytokinin_DH_FAD/cytokin-bd IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR006094, Oxid_FAD_bind_N IPR006093, Oxy_OxRdtase_FAD_BS |
Pfami | View protein in Pfam PF09265, Cytokin-bind, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit PS00862, OX2_COVAL_FAD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9LTS3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASYNLRSQV RLIAITIVII ITLSTPITTN TSPQPWNILS HNEFAGKLTS
60 70 80 90 100
SSSSVESAAT DFGHVTKIFP SAVLIPSSVE DITDLIKLSF DSQLSFPLAA
110 120 130 140 150
RGHGHSHRGQ ASAKDGVVVN MRSMVNRDRG IKVSRTCLYV DVDAAWLWIE
160 170 180 190 200
VLNKTLELGL TPVSWTDYLY LTVGGTLSNG GISGQTFRYG PQITNVLEMD
210 220 230 240 250
VITGKGEIAT CSKDMNSDLF FAVLGGLGQF GIITRARIKL EVAPKRAKWL
260 270 280 290 300
RFLYIDFSEF TRDQERVISK TDGVDFLEGS IMVDHGPPDN WRSTYYPPSD
310 320 330 340 350
HLRIASMVKR HRVIYCLEVV KYYDETSQYT VNEEMEELSD SLNHVRGFMY
360 370 380 390 400
EKDVTYMDFL NRVRTGELNL KSKGQWDVPH PWLNLFVPKT QISKFDDGVF
410 420 430 440 450
KGIILRNNIT SGPVLVYPMN RNKWNDRMSA AIPEEDVFYA VGFLRSAGFD
460 470 480 490 500
NWEAFDQENM EILKFCEDAN MGVIQYLPYH SSQEGWVRHF GPRWNIFVER
510 520
KYKYDPKMIL SPGQNIFQKI NSS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8BER3 | A0A1P8BER3_ARATH | Cytokinin dehydrogenase | CKX3 ATCKX3, CYTOKININ OXIDASE, cytokinin oxidase 3, MHM17.8, MHM17_8 | 423 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF303979 mRNA Translation: AAG30906.1 AB024035 Genomic DNA Translation: BAA97027.1 CP002688 Genomic DNA Translation: AED96828.1 |
RefSeqi | NP_200507.1, NM_125079.3 |
Genome annotation databases
EnsemblPlantsi | AT5G56970.1; AT5G56970.1; AT5G56970 |
GeneIDi | 835799 |
Gramenei | AT5G56970.1; AT5G56970.1; AT5G56970 |
KEGGi | ath:AT5G56970 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF303979 mRNA Translation: AAG30906.1 AB024035 Genomic DNA Translation: BAA97027.1 CP002688 Genomic DNA Translation: AED96828.1 |
RefSeqi | NP_200507.1, NM_125079.3 |
3D structure databases
SMRi | Q9LTS3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 21043, 2 interactors |
STRINGi | 3702.AT5G56970.1 |
PTM databases
iPTMneti | Q9LTS3 |
Proteomic databases
PaxDbi | Q9LTS3 |
ProteomicsDBi | 222614 |
Genome annotation databases
EnsemblPlantsi | AT5G56970.1; AT5G56970.1; AT5G56970 |
GeneIDi | 835799 |
Gramenei | AT5G56970.1; AT5G56970.1; AT5G56970 |
KEGGi | ath:AT5G56970 |
Organism-specific databases
Araporti | AT5G56970 |
TAIRi | locus:2164615, AT5G56970 |
Phylogenomic databases
eggNOGi | KOG1231, Eukaryota |
HOGENOMi | CLU_024955_1_0_1 |
InParanoidi | Q9LTS3 |
OrthoDBi | 350817at2759 |
PhylomeDBi | Q9LTS3 |
Enzyme and pathway databases
BioCyci | ARA:AT5G56970-MONOMER |
BRENDAi | 1.5.99.12, 399 |
SABIO-RKi | Q9LTS3 |
Miscellaneous databases
PROi | PR:Q9LTS3 |
Gene expression databases
ExpressionAtlasi | Q9LTS3, baseline and differential |
Genevisiblei | Q9LTS3, AT |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.40.462.10, 1 hit |
InterProi | View protein in InterPro IPR016170, Cytok_DH_C_sf IPR015345, Cytokinin_DH_FAD/cytokin-bd IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR006094, Oxid_FAD_bind_N IPR006093, Oxy_OxRdtase_FAD_BS |
Pfami | View protein in Pfam PF09265, Cytokin-bind, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit PS00862, OX2_COVAL_FAD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CKX3_ARATH | |
Accessioni | Q9LTS3Primary (citable) accession number: Q9LTS3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2002 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families