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Protein

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and protein-tyrosine-phosphatase PTEN2A

Gene

PTEN2A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds phosphatidic acid. Protein tyrosine phosphatase that exhibits also lipid phosphatase activity. Hydrolyzed poorly p-nitrophenyl phosphate (p-NPP). Can use PtdIns isomers as substrates. Removes efficiently phosphate from the D3 position of the inositol ring, less from the D4 position and not at all from the D5 position on monophosphorylated PtdIns isomers (PIPs). The presence of a phosphate group in the D5 position on PIP2 isomers reduces lipid phosphatase activity. Mostly active on PtdIns3P and PtdIns(3,4)P2, to a lower extent, on PtdIns4P and PtdIns(3,5)P2, but barely against PtdIns(3,4,5)P3 as substrate.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation1 Publication
Phosphatidylinositol 3,4,5-trisphosphate + H2O = phosphatidylinositol 4,5-bisphosphate + phosphate.

Kineticsi

  1. KM=8.5 mM for p-NPP1 Publication
  1. Vmax=206 nmol/min/mg enzyme with p-NPP as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei263Phosphocysteine intermediatePROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

  • peptidyl-tyrosine dephosphorylation Source: TAIR
  • phosphatidylinositol dephosphorylation Source: TAIR
  • response to osmotic stress Source: UniProtKB
  • response to salt stress Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processLipid metabolism, Phospholipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G19420-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and protein-tyrosine-phosphatase PTEN2ACurated (EC:3.1.3.48PROSITE-ProRule annotation1 Publication, EC:3.1.3.671 Publication)
Alternative name(s):
Protein PHOSPHATASE AND TENSIN HOMOLOG 2-a1 Publication
Short name:
AtPTEN21 Publication
Short name:
AtPTEN2a1 Publication
Gene namesi
Name:PTEN2A1 Publication
Synonyms:PTEN21 Publication
Ordered Locus Names:At3g19420Imported
ORF Names:MLD14.151 Publication
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G19420
TAIRilocus:2090659 AT3G19420

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi263C → S: Loss of phosphatase activity. 1 Publication1
Mutagenesisi267 – 268MA → KG: Reduced phosphatase activity toward PtdIns(3)P and PtdIns(4)P, but strongly increased activity for PtdIns(3,4)P(2), PtdIns(3,5)P(2) and PtdIns(3,4,5)P(3) as substrates. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004351681 – 611Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and protein-tyrosine-phosphatase PTEN2AAdd BLAST611

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei91PhosphoserineCombined sources1
Modified residuei509PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LT75
PRIDEiQ9LT75

PTM databases

iPTMnetiQ9LT75

Expressioni

Tissue specificityi

Expressed in seedlings, roots, stems, leaves, flowers and siliques. However, at protein level, not observed in older leaves and mature siliques.1 Publication

Inductioni

Accumulates in response to salt (e.g. NaCl) and osmotic stresses (e.g. mannitol).1 Publication

Gene expression databases

ExpressionAtlasiQ9LT75 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G19420.1

Structurei

3D structure databases

ProteinModelPortaliQ9LT75
SMRiQ9LT75
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini145 – 324Phosphatase tensin-typePROSITE-ProRule annotationAdd BLAST180
Domaini331 – 458C2 tensin-typePROSITE-ProRule annotationAdd BLAST128

Sequence similaritiesi

Belongs to the PTEN phosphatase protein family.1 Publication

Phylogenomic databases

eggNOGiKOG2283 Eukaryota
COG2453 LUCA
HOGENOMiHOG000030169
KOiK01110
OMAiNEKSAFA
OrthoDBiEOG093604TH
PhylomeDBiQ9LT75

Family and domain databases

Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR029021 Prot-tyrosine_phosphatase-like
IPR014020 Tensin_C2-dom
IPR029023 Tensin_phosphatase
IPR016130 Tyr_Pase_AS
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM01326 PTEN_C2, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS51182 C2_TENSIN, 1 hit
PS51181 PPASE_TENSIN, 1 hit
PS00383 TYR_PHOSPHATASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LT75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSESPNLPA AAGTVPDNHP PPPPVVTAAE AGSDDSPKGV ASKLSAAGIS
60 70 80 90 100
NWAKNLKVPQ PFASTQNDSG VENTEKSAFA KFTSGLGIRL SPKSPQTNDT
110 120 130 140 150
TTEGTSSATE SSFIGTITKG LVDTSKNAVK AVQVKARHAV SQNKRRYQEG
160 170 180 190 200
GFDLDLTYIT ENIIAMGFPA GDMSSGFFGY VEGFYRNQME EVINFLETQH
210 220 230 240 250
KGKYKVYNLC SERLYDVSLF EGKVASFPFD DHNCPPIHLV TSFCQSAYSW
260 270 280 290 300
LKEDIENVVV VHCKAGMART GLMICSLLLY LKFFPTAEEC MDFYNQKRCV
310 320 330 340 350
DGKGLVLPSQ IRYVKYFERI LTYFNGENQP GRRCMLRGFR LHRCPYWIRP
360 370 380 390 400
SITISDHNGV LFTTKKHPRT KDLSPEDFWF SAPKKGVMVF ALPGEPGLTE
410 420 430 440 450
LAGDFKIQFH DRQGDFYCWL NTTMMENRVI LKTSELDGFD KRKLPSPGFM
460 470 480 490 500
VEVVLADINA TIPTNPSSET ASKTPEETSA ANSSPVDGSA SVPGPDKETE
510 520 530 540 550
NPDKDDVFSD NEGDSTGPTK TTSSASSQTP EAKKSADETA VLTKATEKVS
560 570 580 590 600
ISGNKGSSQP VQGVTVSKGE ATEKPSGAGV NASSSSESEF KVMAADASVF
610
SFGDEDDFES D
Length:611
Mass (Da):66,430
Last modified:October 1, 2000 - v1
Checksum:iCB4FB3CFE0C6C577
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19 – 23HPPPP → PPPPL in AAO13749 (Ref. 1) Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF268257 mRNA Translation: AAO13749.1
AB025624 Genomic DNA Translation: BAB02466.1
CP002686 Genomic DNA Translation: AEE76238.1
AY070042 mRNA Translation: AAL49799.1
RefSeqiNP_188573.1, NM_112829.5
UniGeneiAt.5710

Genome annotation databases

EnsemblPlantsiAT3G19420.1; AT3G19420.1; AT3G19420
GeneIDi821476
GrameneiAT3G19420.1; AT3G19420.1; AT3G19420
KEGGiath:AT3G19420

Similar proteinsi

Entry informationi

Entry nameiPTN2A_ARATH
AccessioniPrimary (citable) accession number: Q9LT75
Secondary accession number(s): Q8GZT8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2016
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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