UniProtKB - Q9LT31 (VPS9A_ARATH)
Vacuolar protein sorting-associated protein 9A
VPS9A
Functioni
Functions as guanine nucleotide exchange factor (GEF) for Rab small GTPases. Activates specifically RABF1, RABF2A and RABF2B proteins. Required for early stages of embryogenesis, cytokinesis, embryogenesis, and organ development. Is essential for the establishment or maintenance of the polar localization of the auxin efflux carrier PIN1.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 180 | GTPCombined sources1 Publication | 1 | |
Binding sitei | 185 | GTPCombined sources1 Publication | 1 |
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB-KW
- GTP binding Source: UniProtKB-KW
- guanyl-nucleotide exchange factor activity Source: TAIR
- small GTPase binding Source: GO_Central
GO - Biological processi
- cell plate assembly Source: TAIR
- cell wall biogenesis Source: TAIR
- embryo development ending in seed dormancy Source: TAIR
- late endosome to vacuole transport Source: TAIR
- post-embryonic root development Source: TAIR
Keywordsi
Molecular function | GTPase activation |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 9ACuratedShort name: AtVPS9a1 Publication |
Gene namesi | Name:VPS9A1 Publication Synonyms:VPS9Curated Ordered Locus Names:At3g19770Imported ORF Names:MMB12.26Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G19770 |
TAIRi | locus:2092211, AT3G19770 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Other locations
- endocytic vesicle Source: GO_Central
Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 184 | A → K: Loss of interaction with RABF2B. 1 Publication | 1 | |
Mutagenesisi | 185 | D → A: Loss of interaction with RABF2B. Decreases GEF activity 12-fold. 1 Publication | 1 | |
Mutagenesisi | 185 | D → E: Loss of interaction with RABF2B. 1 Publication | 1 | |
Mutagenesisi | 185 | D → N: Weakens interaction with RABF2B. Increases GEF activity. 1 Publication | 1 | |
Mutagenesisi | 225 | Y → A: Loss of interaction with RABF2B. Decreases GEF activity 25-fold. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000406607 | 1 – 520 | Vacuolar protein sorting-associated protein 9AAdd BLAST | 520 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 330 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9LT31 |
PRIDEi | Q9LT31 |
ProteomicsDBi | 242780 [Q9LT31-1] |
PTM databases
iPTMneti | Q9LT31 |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | Q9LT31, baseline and differential |
Genevisiblei | Q9LT31, AT |
Interactioni
Subunit structurei
Homodimer. The homodimer interacts with RABF2B.
Interacts with RABF1 and RABF2A.
2 PublicationsGO - Molecular functioni
- small GTPase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 6846, 1 interactor |
STRINGi | 3702.AT3G19770.1 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9LT31 |
SMRi | Q9LT31 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9LT31 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 102 – 246 | VPS9PROSITE-ProRule annotationAdd BLAST | 145 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 267 – 331 | DisorderedSequence analysisAdd BLAST | 65 | |
Regioni | 396 – 433 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 464 – 520 | DisorderedSequence analysisAdd BLAST | 57 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 283 – 327 | Polar residuesSequence analysisAdd BLAST | 45 | |
Compositional biasi | 396 – 411 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 412 – 433 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 464 – 513 | Basic and acidic residuesSequence analysisAdd BLAST | 50 |
Phylogenomic databases
eggNOGi | KOG2319, Eukaryota |
HOGENOMi | CLU_036671_1_0_1 |
InParanoidi | Q9LT31 |
OMAi | KIQEYPF |
PhylomeDBi | Q9LT31 |
Family and domain databases
Gene3Di | 1.20.1050.80, 1 hit |
InterProi | View protein in InterPro IPR041545, DUF5601 IPR003123, VPS9 IPR045046, Vps9-like IPR037191, VPS9_dom_sf |
PANTHERi | PTHR23101, PTHR23101, 1 hit |
Pfami | View protein in Pfam PF18151, DUF5601, 1 hit PF02204, VPS9, 1 hit |
SMARTi | View protein in SMART SM00167, VPS9, 1 hit |
SUPFAMi | SSF109993, SSF109993, 1 hit |
PROSITEi | View protein in PROSITE PS51205, VPS9, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketThis entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MENTDVFLGL HDFLERMRKP SAGDFVKSIK SFIVSFSNNA PDPEKDCAMV
60 70 80 90 100
QEFFSKMEAA FRAHPLWSGC SEEELDSAGD GLEKYVMTKL FTRVFASNTE
110 120 130 140 150
EVIADEKLFQ KMSLVQQFIS PENLDIQPTF QNESSWLLAQ KELQKINMYK
160 170 180 190 200
APRDKLVCIL NCCKVINNLL LNASIASNEN APGADEFLPV LIYVTIKANP
210 220 230 240 250
PQLHSNLLYI QRYRRESKLV GEAAYFFTNI LSAESFISNI DAKSISLDEA
260 270 280 290 300
EFEKNMESAR ARISGLDSQT YQTGHGSAPP PRDESTLQKT QSLNPKRENT
310 320 330 340 350
LFQSKSSDSL SGTNELLNIN SETPMKKAES ISDLENKGAT LLKDTEPSKV
360 370 380 390 400
FQEYPYIFAS AGDLRIGDVE GLLNSYKQLV FKYVCLTKGL GDGTSLAPSS
410 420 430 440 450
SPLQASSGFN TSKESEDHRR SSSDVQMTKE TDRSVDDLIR ALHGEGEDVN
460 470 480 490 500
NLSDVKHEEY GAMLVEGKDE ERDSKVQGEV DAKDIELMKQ IPKREGDNSS
510 520
SRPAEDEDVG SKQPVTEASE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4JCD2 | F4JCD2_ARATH | Vacuolar sorting protein 9 (VPS9) d... | VPS9A ATVPS9A, MMB12.26, VPS9, At3g19770 | 384 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB025631, AP000417 Genomic DNA Translation: BAB01291.1 CP002686 Genomic DNA Translation: AEE76284.1 AY045810 mRNA Translation: AAK76484.1 AY079338 mRNA Translation: AAL85069.1 |
RefSeqi | NP_566645.1, NM_112867.5 [Q9LT31-1] |
Genome annotation databases
EnsemblPlantsi | AT3G19770.1; AT3G19770.1; AT3G19770 [Q9LT31-1] |
GeneIDi | 821514 |
Gramenei | AT3G19770.1; AT3G19770.1; AT3G19770 [Q9LT31-1] |
KEGGi | ath:AT3G19770 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB025631, AP000417 Genomic DNA Translation: BAB01291.1 CP002686 Genomic DNA Translation: AEE76284.1 AY045810 mRNA Translation: AAK76484.1 AY079338 mRNA Translation: AAL85069.1 |
RefSeqi | NP_566645.1, NM_112867.5 [Q9LT31-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2EFC | X-ray | 2.09 | A/C | 1-265 | [»] | |
2EFD | X-ray | 3.00 | A/C | 1-265 | [»] | |
2EFE | X-ray | 2.08 | A/C | 1-265 | [»] | |
2EFH | X-ray | 2.10 | A/C | 1-265 | [»] | |
4G01 | X-ray | 2.20 | A | 1-265 | [»] | |
AlphaFoldDBi | Q9LT31 | |||||
SMRi | Q9LT31 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 6846, 1 interactor |
STRINGi | 3702.AT3G19770.1 |
PTM databases
iPTMneti | Q9LT31 |
Proteomic databases
PaxDbi | Q9LT31 |
PRIDEi | Q9LT31 |
ProteomicsDBi | 242780 [Q9LT31-1] |
Genome annotation databases
EnsemblPlantsi | AT3G19770.1; AT3G19770.1; AT3G19770 [Q9LT31-1] |
GeneIDi | 821514 |
Gramenei | AT3G19770.1; AT3G19770.1; AT3G19770 [Q9LT31-1] |
KEGGi | ath:AT3G19770 |
Organism-specific databases
Araporti | AT3G19770 |
TAIRi | locus:2092211, AT3G19770 |
Phylogenomic databases
eggNOGi | KOG2319, Eukaryota |
HOGENOMi | CLU_036671_1_0_1 |
InParanoidi | Q9LT31 |
OMAi | KIQEYPF |
PhylomeDBi | Q9LT31 |
Miscellaneous databases
EvolutionaryTracei | Q9LT31 |
PROi | PR:Q9LT31 |
Gene expression databases
ExpressionAtlasi | Q9LT31, baseline and differential |
Genevisiblei | Q9LT31, AT |
Family and domain databases
Gene3Di | 1.20.1050.80, 1 hit |
InterProi | View protein in InterPro IPR041545, DUF5601 IPR003123, VPS9 IPR045046, Vps9-like IPR037191, VPS9_dom_sf |
PANTHERi | PTHR23101, PTHR23101, 1 hit |
Pfami | View protein in Pfam PF18151, DUF5601, 1 hit PF02204, VPS9, 1 hit |
SMARTi | View protein in SMART SM00167, VPS9, 1 hit |
SUPFAMi | SSF109993, SSF109993, 1 hit |
PROSITEi | View protein in PROSITE PS51205, VPS9, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS9A_ARATH | |
Accessioni | Q9LT31Primary (citable) accession number: Q9LT31 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 5, 2011 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references