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Protein

Transcription factor ICE1

Gene

SCRM

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator that regulates the cold-induced transcription of CBF/DREB1 genes. Binds specifically to the MYC recognition sites (5'-CANNTG-3') found in the CBF3/DREB1A promoter. Mediates stomatal differentiation in the epidermis probably by controlling successive roles of SPCH, MUTE, and FAMA.2 Publications

GO - Molecular functioni

  • DNA binding Source: TAIR
  • DNA-binding transcription factor activity Source: TAIR
  • protein dimerization activity Source: InterPro

GO - Biological processi

  • positive regulation of transcription, DNA-templated Source: TAIR
  • response to cold Source: TAIR
  • response to freezing Source: TAIR
  • stomatal lineage progression Source: TAIR

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processStress response, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor ICE1
Alternative name(s):
Basic helix-loop-helix protein 116
Short name:
AtbHLH116
Short name:
bHLH 116
Inducer of CBF expression 1
Transcription factor EN 45
Transcription factor SCREAM
bHLH transcription factor bHLH116
Gene namesi
Name:SCRM
Synonyms:BHLH116, EN45, ICE1
Ordered Locus Names:At3g26744
ORF Names:MDJ14.1, MLJ15.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G26744
TAIRilocus:2090847 AT3G26744

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi236R → H in scrm-D and ice1-1D; cold resistance and excessive stomatal differentiation. Suppresses the cold-induction of CBF3/DREB1A. 2 Publications1
Mutagenesisi312E → G: Loss of excessive stomatal differentiation; when associated with H-236. 1 Publication1
Mutagenesisi393K → R: Loss of sumoylation. Increases freezing sensitivity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272351 – 494Transcription factor ICE1Add BLAST494

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki393Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modificationi

Ubiquitinated. Cold-treatment induced association with the E3 ubiquitin ligase HOS1 targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway. Sumoylated at Lys-393 by SIZ1. Sumoylated ICE1 represses HOS1 and MYB15 and facilitates the positive regulation of CBF3/DREB1A-dependent cold signaling and freezing tolerance.2 Publications

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9LSE2

Expressioni

Tissue specificityi

Widely expressed in the whole plant with high expression in leaves and stem. Broad expression within stomatal cell lineages of leaf epidermis.1 Publication

Inductioni

By high-salt stress, cold stress and abscisic acid (ABA) treatment.

Gene expression databases

ExpressionAtlasiQ9LSE2 baseline and differential
GenevisibleiQ9LSE2 AT

Interactioni

Subunit structurei

Homodimer (Probable). Efficient DNA binding requires dimerization with another bHLH protein (By similarity). Interacts with the C-terminal part of HOS1. Heterodimers with SPCH, MUTE, and FAMA.By similarityCurated3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HOS1Q84JU63EBI-15583266,EBI-15583242

GO - Molecular functioni

Protein-protein interaction databases

BioGridi7617, 20 interactors
DIPiDIP-61175N
IntActiQ9LSE2, 2 interactors
STRINGi3702.AT3G26744.1

Structurei

3D structure databases

ProteinModelPortaliQ9LSE2
SMRiQ9LSE2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini303 – 352bHLHPROSITE-ProRule annotationAdd BLAST50

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili344 – 365Sequence analysisAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi89 – 96Poly-Ser8

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJE0 Eukaryota
ENOG4111GT2 LUCA
HOGENOMiHOG000085036
InParanoidiQ9LSE2
OrthoDBiEOG09360JVO
PhylomeDBiQ9LSE2

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9LSE2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGLDGNNGGG VWLNGGGGER EENEEGSWGR NQEDGSSQFK PMLEGDWFSS
60 70 80 90 100
NQPHPQDLQM LQNQPDFRYF GGFPFNPNDN LLLQHSIDSS SSCSPSQAFS
110 120 130 140 150
LDPSQQNQFL STNNNKGCLL NVPSSANPFD NAFEFGSESG FLNQIHAPIS
160 170 180 190 200
MGFGSLTQLG NRDLSSVPDF LSARSLLAPE SNNNNTMLCG GFTAPLELEG
210 220 230 240 250
FGSPANGGFV GNRAKVLKPL EVLASSGAQP TLFQKRAAMR QSSGSKMGNS
260 270 280 290 300
ESSGMRRFSD DGDMDETGIE VSGLNYESDE INESGKAAES VQIGGGGKGK
310 320 330 340 350
KKGMPAKNLM AERRRRKKLN DRLYMLRSVV PKISKMDRAS ILGDAIDYLK
360 370 380 390 400
ELLQRINDLH NELESTPPGS LPPTSSSFHP LTPTPQTLSC RVKEELCPSS
410 420 430 440 450
LPSPKGQQAR VEVRLREGRA VNIHMFCGRR PGLLLATMKA LDNLGLDVQQ
460 470 480 490
AVISCFNGFA LDVFRAEQCQ EGQEILPDQI KAVLFDTAGY AGMI
Length:494
Mass (Da):53,539
Last modified:October 1, 2000 - v1
Checksum:iDCD56016F49E105B
GO
Isoform 2 (identifier: Q9LSE2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-363: MDRASILGDAIDYLKELLQRINDLHNEL → VNTYFVSFISLRACLLVAVIENCCVVAL
     364-494: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:363
Mass (Da):39,153
Checksum:iA8C74BE112AEA3AF
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1I9LRH4A0A1I9LRH4_ARATH
Basic helix-loop-helix (BHLH) DNA-b...
ICE1 ATICE1, INDUCER OF CBF EXPRESSION 1, SCREAM, SCRM, At3g26744
476Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93C → R in BAF00372 (Ref. 6) Curated1
Sequence conflicti190G → C in BAF00372 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020190336 – 363MDRAS…LHNEL → VNTYFVSFISLRACLLVAVI ENCCVVAL in isoform 2. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_020191364 – 494Missing in isoform 2. 1 PublicationAdd BLAST131

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY195621 mRNA Translation: AAP14668.1
AB026648, AB016889 Genomic DNA Translation: BAB01738.1
CP002686 Genomic DNA Translation: AEE77208.1
CP002686 Genomic DNA Translation: AEE77209.1
CP002686 Genomic DNA Translation: AEE77210.1
CP002686 Genomic DNA Translation: ANM65181.1
AY079016 mRNA Translation: AAL84972.1
BT020379 mRNA Translation: AAV85734.1
AK228443 mRNA Translation: BAF00372.1
RefSeqiNP_001030774.1, NM_001035697.2 [Q9LSE2-1]
NP_001030776.2, NM_001035699.2 [Q9LSE2-1]
NP_001327170.1, NM_001338833.1 [Q9LSE2-2]
NP_189309.2, NM_113586.4 [Q9LSE2-1]
UniGeneiAt.25302

Genome annotation databases

EnsemblPlantsiAT3G26744.1; AT3G26744.1; AT3G26744 [Q9LSE2-1]
AT3G26744.2; AT3G26744.2; AT3G26744 [Q9LSE2-1]
AT3G26744.4; AT3G26744.4; AT3G26744 [Q9LSE2-1]
AT3G26744.5; AT3G26744.5; AT3G26744 [Q9LSE2-2]
GeneIDi822287
GrameneiAT3G26744.1; AT3G26744.1; AT3G26744 [Q9LSE2-1]
AT3G26744.2; AT3G26744.2; AT3G26744 [Q9LSE2-1]
AT3G26744.4; AT3G26744.4; AT3G26744 [Q9LSE2-1]
AT3G26744.5; AT3G26744.5; AT3G26744 [Q9LSE2-2]
KEGGiath:AT3G26744

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY195621 mRNA Translation: AAP14668.1
AB026648, AB016889 Genomic DNA Translation: BAB01738.1
CP002686 Genomic DNA Translation: AEE77208.1
CP002686 Genomic DNA Translation: AEE77209.1
CP002686 Genomic DNA Translation: AEE77210.1
CP002686 Genomic DNA Translation: ANM65181.1
AY079016 mRNA Translation: AAL84972.1
BT020379 mRNA Translation: AAV85734.1
AK228443 mRNA Translation: BAF00372.1
RefSeqiNP_001030774.1, NM_001035697.2 [Q9LSE2-1]
NP_001030776.2, NM_001035699.2 [Q9LSE2-1]
NP_001327170.1, NM_001338833.1 [Q9LSE2-2]
NP_189309.2, NM_113586.4 [Q9LSE2-1]
UniGeneiAt.25302

3D structure databases

ProteinModelPortaliQ9LSE2
SMRiQ9LSE2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7617, 20 interactors
DIPiDIP-61175N
IntActiQ9LSE2, 2 interactors
STRINGi3702.AT3G26744.1

Proteomic databases

PaxDbiQ9LSE2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G26744.1; AT3G26744.1; AT3G26744 [Q9LSE2-1]
AT3G26744.2; AT3G26744.2; AT3G26744 [Q9LSE2-1]
AT3G26744.4; AT3G26744.4; AT3G26744 [Q9LSE2-1]
AT3G26744.5; AT3G26744.5; AT3G26744 [Q9LSE2-2]
GeneIDi822287
GrameneiAT3G26744.1; AT3G26744.1; AT3G26744 [Q9LSE2-1]
AT3G26744.2; AT3G26744.2; AT3G26744 [Q9LSE2-1]
AT3G26744.4; AT3G26744.4; AT3G26744 [Q9LSE2-1]
AT3G26744.5; AT3G26744.5; AT3G26744 [Q9LSE2-2]
KEGGiath:AT3G26744

Organism-specific databases

AraportiAT3G26744
TAIRilocus:2090847 AT3G26744

Phylogenomic databases

eggNOGiENOG410IJE0 Eukaryota
ENOG4111GT2 LUCA
HOGENOMiHOG000085036
InParanoidiQ9LSE2
OrthoDBiEOG09360JVO
PhylomeDBiQ9LSE2

Miscellaneous databases

PROiPR:Q9LSE2

Gene expression databases

ExpressionAtlasiQ9LSE2 baseline and differential
GenevisibleiQ9LSE2 AT

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiICE1_ARATH
AccessioniPrimary (citable) accession number: Q9LSE2
Secondary accession number(s): Q0WR77, Q2V3S3, Q5PNR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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