UniProtKB - Q9LQQ9 (MPK13_ARATH)
Protein
Mitogen-activated protein kinase 13
Gene
MPK13
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
MKK6-MPK13 module positively regulates lateral root formation.1 Publication
Catalytic activityi
Activity regulationi
Activated by threonine and tyrosine phosphorylation (By similarity). Activated by the MAP kinase kinase MKK6 in vitro.By similarity2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 62 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 159 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 39 – 47 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase activity Source: TAIR
GO - Biological processi
- intracellular signal transduction Source: GO_Central
- lateral root formation Source: UniProtKB
- regulation of gene expression Source: GO_Central
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase 13 (EC:2.7.11.24)Short name: AtMPK13 Short name: MAP kinase 13 |
Gene namesi | Name:MPK13 Ordered Locus Names:At1g07880 ORF Names:F24B9.3 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G07880 |
TAIRi | locus:2026484, AT1G07880 |
Subcellular locationi
Pathology & Biotechi
Disruption phenotypei
RNAi MPK13 displays fewer lateral roots.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245813 | 1 – 363 | Mitogen-activated protein kinase 13Add BLAST | 363 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 191 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 193 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 196 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9LQQ9 |
PRIDEi | Q9LQQ9 |
ProteomicsDBi | 238275 [Q9LQQ9-1] |
PTM databases
iPTMneti | Q9LQQ9 |
Expressioni
Tissue specificityi
Expressed in roots, stems and flower buds.1 Publication
Gene expression databases
ExpressionAtlasi | Q9LQQ9, baseline and differential |
Genevisiblei | Q9LQQ9, AT |
Interactioni
Subunit structurei
Interacts with MKK6.
1 PublicationBinary interactionsi
Q9LQQ9
With | #Exp. | IntAct |
---|---|---|
MKK2 [Q9S7U9] | 2 | EBI-2358762,EBI-994350 |
Protein-protein interaction databases
BioGRIDi | 22544, 2 interactors |
IntActi | Q9LQQ9, 2 interactors |
MINTi | Q9LQQ9 |
STRINGi | 3702.AT1G07880.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 319 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 287 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 191 – 193 | TXY | 3 |
Domaini
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Sequence similaritiesi
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
InParanoidi | Q9LQQ9 |
OMAi | ARSHMLR |
OrthoDBi | 741207at2759 |
PhylomeDBi | Q9LQQ9 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9LQQ9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEKREDGGIL TYDGRYVMYN VLGNIFELSS KYIPPIEPIG RGAYGIVCCA
60 70 80 90 100
TNSETNEEVA IKKIANAFDN RVDAKRTLRE IKLLSHMDHD NVIKIKDIIE
110 120 130 140 150
LPEKERFEDV YIVYELMDTD LHQIIRSTQT LTDDHCQYFL YQILRGLKYI
160 170 180 190 200
HSANVLHRDL KPSNLVLNTN CDLKICDFGL ARTSNETEIM TEYVVTRWYR
210 220 230 240 250
APELLLNSSE YTGAIDIWSV GCIFMEILRR ETLFPGKDYV QQLKLITELL
260 270 280 290 300
GSPDDSDLDF LRSDNARKYV KQLPHVQKQS FREKFPNISP MALDLAEKML
310 320 330 340 350
VFDPSKRITV DEALKQPYLA SLHEINEEPT CPTPFSFDFE ETALDEQDIK
360
ELVWRESLHF KNM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Isoform 2 (identifier: Q9LQQ9-2) | |||||
Sequence conflicti | 243 | L → P in BX818168 (PubMed:14993207).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036334 | 249 – 254 | LLGSPD → VSKLKP in isoform 2. 1 Publication | 6 | |
Alternative sequenceiVSP_036335 | 255 – 363 | Missing in isoform 2. 1 PublicationAdd BLAST | 109 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007583 Genomic DNA Translation: AAF75067.1 CP002684 Genomic DNA Translation: AEE28197.1 CP002684 Genomic DNA Translation: AEE28198.1 BX818168 mRNA No translation available. BT015822 mRNA Translation: AAU94385.1 |
PIRi | C86214 |
RefSeqi | NP_001030990.1, NM_001035913.2 [Q9LQQ9-1] NP_172266.2, NM_100662.4 [Q9LQQ9-2] |
Genome annotation databases
EnsemblPlantsi | AT1G07880.1; AT1G07880.1; AT1G07880 [Q9LQQ9-2] AT1G07880.2; AT1G07880.2; AT1G07880 [Q9LQQ9-1] |
GeneIDi | 837303 |
Gramenei | AT1G07880.1; AT1G07880.1; AT1G07880 [Q9LQQ9-2] AT1G07880.2; AT1G07880.2; AT1G07880 [Q9LQQ9-1] |
KEGGi | ath:AT1G07880 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007583 Genomic DNA Translation: AAF75067.1 CP002684 Genomic DNA Translation: AEE28197.1 CP002684 Genomic DNA Translation: AEE28198.1 BX818168 mRNA No translation available. BT015822 mRNA Translation: AAU94385.1 |
PIRi | C86214 |
RefSeqi | NP_001030990.1, NM_001035913.2 [Q9LQQ9-1] NP_172266.2, NM_100662.4 [Q9LQQ9-2] |
3D structure databases
SMRi | Q9LQQ9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 22544, 2 interactors |
IntActi | Q9LQQ9, 2 interactors |
MINTi | Q9LQQ9 |
STRINGi | 3702.AT1G07880.2 |
PTM databases
iPTMneti | Q9LQQ9 |
Proteomic databases
PaxDbi | Q9LQQ9 |
PRIDEi | Q9LQQ9 |
ProteomicsDBi | 238275 [Q9LQQ9-1] |
Genome annotation databases
EnsemblPlantsi | AT1G07880.1; AT1G07880.1; AT1G07880 [Q9LQQ9-2] AT1G07880.2; AT1G07880.2; AT1G07880 [Q9LQQ9-1] |
GeneIDi | 837303 |
Gramenei | AT1G07880.1; AT1G07880.1; AT1G07880 [Q9LQQ9-2] AT1G07880.2; AT1G07880.2; AT1G07880 [Q9LQQ9-1] |
KEGGi | ath:AT1G07880 |
Organism-specific databases
Araporti | AT1G07880 |
TAIRi | locus:2026484, AT1G07880 |
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
InParanoidi | Q9LQQ9 |
OMAi | ARSHMLR |
OrthoDBi | 741207at2759 |
PhylomeDBi | Q9LQQ9 |
Miscellaneous databases
PROi | PR:Q9LQQ9 |
Gene expression databases
ExpressionAtlasi | Q9LQQ9, baseline and differential |
Genevisiblei | Q9LQQ9, AT |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MPK13_ARATH | |
Accessioni | Q9LQQ9Primary (citable) accession number: Q9LQQ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families