UniProtKB - Q9LM33 (MPK8_ARATH)
Protein
Mitogen-activated protein kinase 8
Gene
MPK8
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
MKK3-MPK8 and CAMs-MPK8 modules negatively regulates ROS accumulation through controlling expression of the RBOHD gene during wounding.1 Publication
Catalytic activityi
Activity regulationi
Activated by threonine and tyrosine phosphorylation (By similarity). Activated by two independent mechanisms, the binding of CAMs in a calcium-dependent manner and the phosphorylation by MAP kinase kinase MKK3. Activated in response to mechanical wounding, hydrogen peroxide and jasmonic acid (JA).By similarity1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 133 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 230 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 110 – 118 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calmodulin binding Source: TAIR
- MAP kinase activity Source: TAIR
GO - Biological processi
- intracellular signal transduction Source: GO_Central
- regulation of gene expression Source: GO_Central
- response to hydrogen peroxide Source: TAIR
- response to jasmonic acid Source: TAIR
- response to reactive oxygen species Source: TAIR
- response to wounding Source: TAIR
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase 8 (EC:2.7.11.24)Short name: AtMPK8 Short name: MAP kinase 8 |
Gene namesi | Name:MPK8 Ordered Locus Names:At1g18150 ORF Names:T10F20.15, T10O22.12 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G18150 |
TAIRi | locus:2194040, AT1G18150 |
Subcellular locationi
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- cytoplasm Source: GO_Central
Pathology & Biotechi
Disruption phenotypei
More susceptible to oxidative stress.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 133 | K → M: Abolishes CAM4-dependent activity. 1 Publication | 1 | |
Mutagenesisi | 134 | K → R: Abolishes CAM4-dependent activity. 1 Publication | 1 | |
Mutagenesisi | 266 | T → A: Do not affect CAM4-dependent activity; when associated with F-268. 1 Publication | 1 | |
Mutagenesisi | 268 | Y → F: Do not affect CAM4-dependent activity; when associated with A-266. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245808 | 1 – 589 | Mitogen-activated protein kinase 8Add BLAST | 589 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 266 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 268 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 271 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Dually phosphorylated on Thr-266 and Tyr-268, which activates the enzyme (By similarity). Autophosphorylated.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9LM33 |
PRIDEi | Q9LM33 |
ProteomicsDBi | 239069 |
PTM databases
iPTMneti | Q9LM33 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
ExpressionAtlasi | Q9LM33, baseline and differential |
Genevisiblei | Q9LM33, AT |
Interactioni
Subunit structurei
Interacts with CAM3, CAM4 AND CAM7 in an calcium-dependent manner.
1 PublicationGO - Molecular functioni
- calmodulin binding Source: TAIR
Protein-protein interaction databases
BioGRIDi | 23633, 42 interactors |
IntActi | Q9LM33, 1 interactor |
STRINGi | 3702.AT1G18150.3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 104 – 395 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 292 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 266 – 268 | TXY | 3 |
Domaini
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Sequence similaritiesi
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
HOGENOMi | CLU_000288_181_5_1 |
InParanoidi | Q9LM33 |
OMAi | SGEPEFF |
OrthoDBi | 741207at2759 |
PhylomeDBi | Q9LM33 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9LM33-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGGGGNLVDG VRRWLFQRPS SSSSSSSSNN NNNNHEQPIF NSSSFSSSSN
60 70 80 90 100
PNHSANSGEL IIEEDLDFSG LTLINVPKRN HLPMDPHKKG ETEFFTEYGE
110 120 130 140 150
ANRYQIQEVV GKGSYGVVAS AVDSHTGERV AIKKINDVFE HVSDATRILR
160 170 180 190 200
EIKLLRLLRH PDVVEIKHIM LPPSRREFRD IYVVFELMES DLHQVIKAND
210 220 230 240 250
DLTPEHYQFF LYQLLRGLKY VHAANVFHRD LKPKNILANA DCKLKICDFG
260 270 280 290 300
LARVSFNDAP TAIFWTDYVA TRWYRAPELC GSFFSKYTPA IDIWSVGCIF
310 320 330 340 350
AEMLLGKPLF PGKNVVHQLD LMTDFLGTPP PESISRIRNE KARRYLSSMR
360 370 380 390 400
KKQPVPFSHK FPKADPLALR LLERLLAFDP KDRASAEDAL ADPYFSGLSN
410 420 430 440 450
SEREPTTQPI SKLEFDFERK KLVKDDVREL IYREILEYHP QMLEEYLRGG
460 470 480 490 500
DQLSFMYPSG VDRFKRQFAH LEENQGKPGA AGGGRSTALH RHHASLPRER
510 520 530 540 550
VPAPNGETAE ESSDVERRAA AAVASTLESE EADNGGGYSA RNLMKSASIS
560 570 580
GSKCIGVQSK TDKEDTIAEE EDNETVAELT DKVASLHNS
Sequence cautioni
The sequence AAF78388 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAA92222 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 179 – 180 | RD → PH in BAA92222 (Ref. 1) Curated | 2 | |
Sequence conflicti | 365 | D → E in BAA92222 (Ref. 1) Curated | 1 | |
Sequence conflicti | 431 | I → L in BAA92222 (Ref. 1) Curated | 1 | |
Sequence conflicti | 479 | G → V in BAA92222 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB038693 mRNA Translation: BAA92222.1 Frameshift. AC034107 Genomic DNA Translation: AAF97831.1 AC069551 Genomic DNA Translation: AAF78388.1 Sequence problems. CP002684 Genomic DNA Translation: AEE29679.1 CP002684 Genomic DNA Translation: AEE29680.1 CP002684 Genomic DNA Translation: AEE29681.1 AY045931 mRNA Translation: AAK76605.1 AY142618 mRNA Translation: AAN13187.1 |
RefSeqi | NP_001185027.1, NM_001198098.1 NP_173253.1, NM_101675.4 NP_849685.1, NM_179354.3 |
Genome annotation databases
EnsemblPlantsi | AT1G18150.1; AT1G18150.1; AT1G18150 AT1G18150.2; AT1G18150.2; AT1G18150 AT1G18150.3; AT1G18150.3; AT1G18150 |
GeneIDi | 838394 |
Gramenei | AT1G18150.1; AT1G18150.1; AT1G18150 AT1G18150.2; AT1G18150.2; AT1G18150 AT1G18150.3; AT1G18150.3; AT1G18150 |
KEGGi | ath:AT1G18150 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB038693 mRNA Translation: BAA92222.1 Frameshift. AC034107 Genomic DNA Translation: AAF97831.1 AC069551 Genomic DNA Translation: AAF78388.1 Sequence problems. CP002684 Genomic DNA Translation: AEE29679.1 CP002684 Genomic DNA Translation: AEE29680.1 CP002684 Genomic DNA Translation: AEE29681.1 AY045931 mRNA Translation: AAK76605.1 AY142618 mRNA Translation: AAN13187.1 |
RefSeqi | NP_001185027.1, NM_001198098.1 NP_173253.1, NM_101675.4 NP_849685.1, NM_179354.3 |
3D structure databases
SMRi | Q9LM33 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 23633, 42 interactors |
IntActi | Q9LM33, 1 interactor |
STRINGi | 3702.AT1G18150.3 |
PTM databases
iPTMneti | Q9LM33 |
Proteomic databases
PaxDbi | Q9LM33 |
PRIDEi | Q9LM33 |
ProteomicsDBi | 239069 |
Genome annotation databases
EnsemblPlantsi | AT1G18150.1; AT1G18150.1; AT1G18150 AT1G18150.2; AT1G18150.2; AT1G18150 AT1G18150.3; AT1G18150.3; AT1G18150 |
GeneIDi | 838394 |
Gramenei | AT1G18150.1; AT1G18150.1; AT1G18150 AT1G18150.2; AT1G18150.2; AT1G18150 AT1G18150.3; AT1G18150.3; AT1G18150 |
KEGGi | ath:AT1G18150 |
Organism-specific databases
Araporti | AT1G18150 |
TAIRi | locus:2194040, AT1G18150 |
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
HOGENOMi | CLU_000288_181_5_1 |
InParanoidi | Q9LM33 |
OMAi | SGEPEFF |
OrthoDBi | 741207at2759 |
PhylomeDBi | Q9LM33 |
Miscellaneous databases
PROi | PR:Q9LM33 |
Gene expression databases
ExpressionAtlasi | Q9LM33, baseline and differential |
Genevisiblei | Q9LM33, AT |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MPK8_ARATH | |
Accessioni | Q9LM33Primary (citable) accession number: Q9LM33 Secondary accession number(s): Q9LMS2, Q9MB23 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | July 11, 2006 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families