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Protein

Monodehydroascorbate reductase 4, peroxisomal

Gene

MDAR4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process (PubMed:16146528). Involved in the detoxification of H2O2 that escapes the peroxisome and causes oxidative damage to oil bodies (PubMed:17449810).2 Publications

Catalytic activityi

NADH + 2 monodehydroascorbate = NAD+ + 2 ascorbate.Curated

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39FADBy similarity1
Binding sitei46FADBy similarity1
Binding sitei51FADBy similarity1
Binding sitei194NADBy similarity1
Binding sitei200NADBy similarity1
Binding sitei200NADPBy similarity1
Binding sitei259NAD; via amide nitrogenBy similarity1
Binding sitei259NADP; via amide nitrogenBy similarity1
Binding sitei296FADBy similarity1
Binding sitei314FAD; via amide nitrogenBy similarity1
Binding sitei318AscorbateBy similarity1
Binding sitei344FAD; via carbonyl oxygenBy similarity1
Binding sitei344NAD; via carbonyl oxygenBy similarity1
Binding sitei344NADP; via carbonyl oxygenBy similarity1
Binding sitei346AscorbateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15FADBy similarity4
Nucleotide bindingi145 – 146FADBy similarity2
Nucleotide bindingi170 – 176NADBy similarity7
Nucleotide bindingi172 – 176NADPBy similarity5
Nucleotide bindingi312 – 313NADBy similarity2
Nucleotide bindingi312 – 313NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • hydrogen peroxide catabolic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciARA:AT3G27820-MONOMER
BRENDAi1.6.5.4 399

Names & Taxonomyi

Protein namesi
Recommended name:
Monodehydroascorbate reductase 4, peroxisomal1 Publication (EC:1.6.5.4Curated)
Short name:
AtMDAR41 Publication
Gene namesi
Name:MDAR41 Publication
Ordered Locus Names:At3g27820Imported
ORF Names:K16N12.2Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G27820
TAIRilocus:2086430 AT3G27820

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3Cytoplasmic1 Publication3
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Topological domaini25 – 458PeroxisomalCuratedAdd BLAST434
Transmembranei459 – 479HelicalSequence analysisAdd BLAST21
Topological domaini480 – 488CytoplasmicCurated9

Keywords - Cellular componenti

Membrane, Peroxisome

Pathology & Biotechi

Disruption phenotypei

Conditional seedling-lethal phenotype due to the unability of the seeds to break down storage oil to provide carbon skeletons and energy for early seedling growth.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi11G → Q in sdp2-2; loss of ascorbate recycling. 1 Publication1
Mutagenesisi14V → A in sdp2-1; loss of ascorbate recycling. 1 Publication1
Mutagenesisi386G → Q in sdp2-3; loss of ascorbate recycling. 1 Publication1
Mutagenesisi483 – 488Missing : Loss of peroxisomal targeting. 1 Publication6
Mutagenesisi488Missing : No effect on peroxisomal targeting. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002091371 – 488Monodehydroascorbate reductase 4, peroxisomalSequence analysisAdd BLAST488

Proteomic databases

PaxDbiQ9LK94
PRIDEiQ9LK94

PTM databases

iPTMnetiQ9LK94

Expressioni

Gene expression databases

ExpressionAtlasiQ9LK94 baseline and differential
GenevisibleiQ9LK94 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G27820.1

Structurei

3D structure databases

ProteinModelPortaliQ9LK94
SMRiQ9LK94
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi483 – 487Poly-ArgSequence analysis5

Domaini

The C-terminal domain (451-488) is necessary and sufficient for peroxisomal targeting.1 Publication

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1336 Eukaryota
COG0446 LUCA
HOGENOMiHOG000276711
InParanoidiQ9LK94
KOiK08232
OMAiFSAFCYK
OrthoDBiEOG09360BD1
PhylomeDBiQ9LK94

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits
SSF55424 SSF55424, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LK94-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGRAFVYVIL GGGVAAGYAA LEFTRRGVSD GELCIISEEP VAPYERPALS
60 70 80 90 100
KGFLLPEAPA RLPSFHTCVG ANDEKLTPKW YKDHGIELVL GTRVKSVDVR
110 120 130 140 150
RKTLLSSTGE TISYKFLIIA TGARALKLEE FGVEGSDAEN VCYLRDLADA
160 170 180 190 200
NRLATVIQSS SNGNAVVIGG GYIGMECAAS LVINKINVTM VFPEAHCMAR
210 220 230 240 250
LFTPKIASLY EDYYRAKGVK FIKGTVLTSF EFDSNKKVTA VNLKDGSHLP
260 270 280 290 300
ADLVVVGIGI RPNTSLFEGQ LTIEKGGIKV NSRMQSSDSS VYAIGDVATF
310 320 330 340 350
PVKLFGEMRR LEHVDSARKS ARHAVSAIMD PIKTGDFDYL PFFYSRVFAF
360 370 380 390 400
SWQFYGDPTG DVVHFGEYED GKSFGAYWVK KGHLVGSFLE GGTKEEYETI
410 420 430 440 450
SKATQLKPAV TIDLEELERE GLGFAHTVVS QQKVPEVKDI PSAEMVKQSA
460 470 480
SVVMIKKPLY VWHAATGVVV AASVAAFAFW YGRRRRRW
Length:488
Mass (Da):53,526
Last modified:October 1, 2000 - v1
Checksum:iAC21B11991178DFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000371 Genomic DNA Translation: BAB02528.1
CP002686 Genomic DNA Translation: AEE77367.1
AY039980 mRNA Translation: AAK64157.1
AY133800 mRNA Translation: AAM91734.1
RefSeqiNP_189420.1, NM_113698.4
UniGeneiAt.5731

Genome annotation databases

EnsemblPlantsiAT3G27820.1; AT3G27820.1; AT3G27820
GeneIDi822402
GrameneiAT3G27820.1; AT3G27820.1; AT3G27820
KEGGiath:AT3G27820

Similar proteinsi

Entry informationi

Entry nameiMDAR4_ARATH
AccessioniPrimary (citable) accession number: Q9LK94
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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