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UniProtKB - Q9LK90 (ALA8_ARATH)
Protein
Probable phospholipid-transporting ATPase 8
Gene
ALA8
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Involved in transport of phospholipids.
1 PublicationCatalytic activityi
- ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).1 Publication EC:7.6.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 424 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 865 | MagnesiumBy similarity | 1 | |
Metal bindingi | 869 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase-coupled intramembrane lipid transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- magnesium ion binding Source: InterPro
GO - Biological processi
- phospholipid translocation Source: GO_Central
Keywordsi
Molecular function | Translocase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT3G27870-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Probable phospholipid-transporting ATPase 81 Publication (EC:7.6.2.11 Publication)Short name: AtALA81 Publication Alternative name(s): Aminophospholipid flippase 81 Publication |
Gene namesi | Name:ALA81 Publication Ordered Locus Names:At3g27870Imported ORF Names:K16N12.9Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G27870 |
TAIRi | locus:2086465, AT3G27870 |
Subcellular locationi
Other locations
- Membrane Sequence analysis; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 71 | CytoplasmicSequence analysisAdd BLAST | 71 | |
Transmembranei | 72 – 93 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 94 – 97 | ExtracellularSequence analysis | 4 | |
Transmembranei | 98 – 120 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 121 – 299 | CytoplasmicSequence analysisAdd BLAST | 179 | |
Transmembranei | 300 – 321 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 322 – 358 | ExtracellularSequence analysisAdd BLAST | 37 | |
Transmembranei | 359 – 376 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 377 – 920 | CytoplasmicSequence analysisAdd BLAST | 544 | |
Transmembranei | 921 – 940 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 941 – 954 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 955 – 974 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 975 – 1004 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 1005 – 1027 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 1028 – 1040 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 1041 – 1063 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 1064 – 1069 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 1070 – 1090 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1091 – 1107 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 1108 – 1132 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 1133 – 1189 | CytoplasmicSequence analysisAdd BLAST | 57 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046392 | 1 – 1189 | Probable phospholipid-transporting ATPase 8Add BLAST | 1189 |
Proteomic databases
PaxDbi | Q9LK90 |
PTM databases
iPTMneti | Q9LK90 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9LK90, baseline and differential |
Genevisiblei | Q9LK90, AT |
Family & Domainsi
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0206, Eukaryota |
HOGENOMi | CLU_000846_3_1_1 |
InParanoidi | Q9LK90 |
OMAi | PECIHKL |
OrthoDBi | 587717at2759 |
PhylomeDBi | Q9LK90 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9LK90-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGERRKGMK FSKLYSFKCF KPFSREDHSQ IGSRGYSRVV FCNDPDNPEA
60 70 80 90 100
LQLNYRGNYV STTKYTAANF IPKSLFEQFR RVANIYFLVV AFVSFSPLAP
110 120 130 140 150
YTAPSVLAPL LIVIGATMVK EGVEDLRRRK QDVEANNRKV EVLGKTGTFV
160 170 180 190 200
ETKWKNLRVG DLVKVHKDEY FPADLLLLSS SYEDGICYVE TMNLDGETNL
210 220 230 240 250
KLKHALEITS DEESIKNFRG MIKCEDPNEH LYSFVGTLYF EGKQYPLSPQ
260 270 280 290 300
QILLRDSKLK NTDYVYGVVV FTGHDTKVMQ NATDPPSKRS KIEKKMDQII
310 320 330 340 350
YILFSILIVI AFTGSVFFGI ATRRDMSDNG KLRRWYLRPD HTTVFYDPRR
360 370 380 390 400
AVAAAFFHFL TALMLYGYLI PISLYVSIEV VKVLQSIFIN QDQEMYHEET
410 420 430 440 450
DRPARARTSN LNEELGQVDT ILSDKTGTLT CNSMEFVKCS IAGTAYGRGM
460 470 480 490 500
TEVEVALRKQ KGLMTQEEVG DNESLSIKEQ KAVKGFNFWD ERIVDGQWIN
510 520 530 540 550
QPNAELIQKF FRVLAICHTA IPDVNSDTGE ITYEAESPDE AAFVIASREL
560 570 580 590 600
GFEFFSRSQT SISLHEIDHM TGEKVDRVYE LLHVLEFSSS RKRMSVIVRN
610 620 630 640 650
PENRLLLLSK GADSVMFKRL AKHGRQNERE TKEHIKKYAE AGLRTLVITY
660 670 680 690 700
REIDEDEYIV WEEEFLNAKT LVTEDRDALI DAAADKIEKD LILLGSTAVE
710 720 730 740 750
DKLQKGVPDC IEKLSQAGVK IWVLTGDKTE TAINIGYACS LLREGMKQIL
760 770 780 790 800
VTLDSSDIEA LEKQGDKEAV AKASFQSIKK QLREGMSQTA AVTDNSAKEN
810 820 830 840 850
SEMFGLVIDG KSLTYALDSK LEKEFLELAI RCNSVICCRS SPKQKALVTR
860 870 880 890 900
LVKNGTGRTT LAIGDGANDV GMLQEADIGV GISGAEGMQA VMASDFAIAQ
910 920 930 940 950
FRFLERLLLV HGHWCYRRIT LMICYFFYKN LAFGFTLFWY EAYASFSGKP
960 970 980 990 1000
AYNDWYMSCY NVFFTSLPVI ALGVFDQDVS ARLCLKYPLL YQEGVQNVLF
1010 1020 1030 1040 1050
SWERILGWML NGVISSMIIF FLTINTMATQ AFRKDGQVVD YSVLGVTMYS
1060 1070 1080 1090 1100
SVVWTVNCQM AISINYFTWI QHCFIWGSIG VWYLFLVIYG SLPPTFSTTA
1110 1120 1130 1140 1150
FQVFVETSAP SPIYWLVLFL VVFSALLPYF TYRAFQIKFR PMYHDIIVEQ
1160 1170 1180
RRTERTETAP NAVLGELPVQ VEFTLHHLRA NLSRRDSWN
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1I9LQR2 | A0A1I9LQR2_ARATH | Phospholipid-transporting ATPase | At3g27870 | 994 | Annotation score: | ||
A0A1I9LQR1 | A0A1I9LQR1_ARATH | Phospholipid-transporting ATPase | At3g27870 | 998 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP000371 Genomic DNA Translation: BAB02533.1 CP002686 Genomic DNA Translation: AEE77374.1 |
RefSeqi | NP_189425.2, NM_113703.3 |
Genome annotation databases
EnsemblPlantsi | AT3G27870.1; AT3G27870.1; AT3G27870 |
GeneIDi | 822407 |
Gramenei | AT3G27870.1; AT3G27870.1; AT3G27870 |
KEGGi | ath:AT3G27870 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP000371 Genomic DNA Translation: BAB02533.1 CP002686 Genomic DNA Translation: AEE77374.1 |
RefSeqi | NP_189425.2, NM_113703.3 |
3D structure databases
AlphaFoldDBi | Q9LK90 |
SMRi | Q9LK90 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT3G27870.1 |
PTM databases
iPTMneti | Q9LK90 |
Proteomic databases
PaxDbi | Q9LK90 |
Genome annotation databases
EnsemblPlantsi | AT3G27870.1; AT3G27870.1; AT3G27870 |
GeneIDi | 822407 |
Gramenei | AT3G27870.1; AT3G27870.1; AT3G27870 |
KEGGi | ath:AT3G27870 |
Organism-specific databases
Araporti | AT3G27870 |
TAIRi | locus:2086465, AT3G27870 |
Phylogenomic databases
eggNOGi | KOG0206, Eukaryota |
HOGENOMi | CLU_000846_3_1_1 |
InParanoidi | Q9LK90 |
OMAi | PECIHKL |
OrthoDBi | 587717at2759 |
PhylomeDBi | Q9LK90 |
Enzyme and pathway databases
BioCyci | ARA:AT3G27870-MONOMER |
Miscellaneous databases
PROi | PR:Q9LK90 |
Gene expression databases
ExpressionAtlasi | Q9LK90, baseline and differential |
Genevisiblei | Q9LK90, AT |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALA8_ARATH | |
Accessioni | Q9LK90Primary (citable) accession number: Q9LK90 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families