UniProtKB - Q9LJH5 (HAOX1_ARATH)
Peroxisomal (S)-2-hydroxyacid oxidase GLO4
GLO4
Functioni
Oxidase that catalyzes the oxidation of a broad range of 2-hydroxyacids to the corresponding 2-oxoacids, with a reduction of O2 to H2O2. Displays the highest activity with the long-chain fatty acid 2-hydroxydodecanoate and has intermediate activity with 2-hydroxyhexanoate, 2-hydroxyoctanote, and the short-chain hydroxyacid (S)-lactate (L-lactate). With much lower activity, it can also use glycolate, leucic acid, valic acid, and isoleucic acid as substrates in vitro. Cannot use 2-hydroxyhexadecanoate or D-lactate as substrates. May be involved in a general medium- and long-chain fatty acid catabolic pathway such as alpha-oxidation.
1 PublicationCatalytic activityi
- EC:1.1.3.151 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Cofactori
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 107 | FMNBy similarity | 1 | |
Binding sitei | 130 | 2-oxocarboxylatePROSITE-ProRule annotation | 1 | |
Binding sitei | 156 | FMNBy similarity | 1 | |
Binding sitei | 165 | 2-oxocarboxylatePROSITE-ProRule annotation | 1 | |
Binding sitei | 228 | FMNBy similarity | 1 | |
Binding sitei | 250 | FMNBy similarity | 1 | |
Active sitei | 252 | Proton acceptorBy similarity | 1 | |
Binding sitei | 255 | 2-oxocarboxylatePROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 78 – 80 | FMNBy similarity | 3 | |
Nucleotide bindingi | 128 – 130 | FMNBy similarity | 3 | |
Nucleotide bindingi | 283 – 287 | FMNBy similarity | 5 | |
Nucleotide bindingi | 306 – 307 | FMNBy similarity | 2 |
GO - Molecular functioni
- (S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
- FMN binding Source: InterPro
GO - Biological processi
- defense response to bacterium Source: TAIR
- hydrogen peroxide biosynthetic process Source: TAIR
- oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
BioCyci | ARA:AT3G14130-MONOMER |
BRENDAi | 1.1.3.15, 399 |
UniPathwayi | UPA00199 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxisomal (S)-2-hydroxyacid oxidase GLO4 (EC:1.1.3.151 Publication)Alternative name(s): Glycolate oxidase 4 Short name: AtGLO4 Short name: GOX 4 lHAOX11 Publication |
Gene namesi | Name:GLO4 Ordered Locus Names:At3g14130 ORF Names:MAG2.2 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G14130 |
TAIRi | locus:2087487, AT3G14130 |
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Peroxisome
- peroxisome Source: TAIR
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000403407 | 1 – 363 | Peroxisomal (S)-2-hydroxyacid oxidase GLO4Add BLAST | 363 |
Proteomic databases
PaxDbi | Q9LJH5 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9LJH5, baseline and differential |
Genevisiblei | Q9LJH5, AT |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 3702.AT3G14130.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 357 | FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST | 357 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 361 – 363 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
HOGENOMi | CLU_020639_6_1_1 |
InParanoidi | Q9LJH5 |
OMAi | FQYEIYL |
OrthoDBi | 879633at2759 |
PhylomeDBi | Q9LJH5 |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MDQIVNVDEF QELAKQALPK MYYDFYNGGA EDQHTLNENV QAFRRIMFRP
60 70 80 90 100
RVLVDVSNID MSTSMLGYPI SAPIMIAPTA MHKLAHPKGE IATAKAAAAC
110 120 130 140 150
NTIMIVSFMS TCTIEEVASS CNAVRFLQIY VYKRRDVTAQ IVKRAEKAGF
160 170 180 190 200
KAIVLTVDVP RLGRREADIK NKMISPQLKN FEGLVSTEVR PNEGSGVEAF
210 220 230 240 250
ASSAFDASLS WKDIEWLRSI TKLPILVKGL LTREDALKAV EAGVDGIVVS
260 270 280 290 300
NHGARQLDYS PATITVLEEV VHAVKGRIPV LLDGGVRRGT DVFKALALGA
310 320 330 340 350
QAVLIGRPIV YGLAAKGEDG VKKVIDMLKN EFEITMALSG CPTIDDVTRN
360
HVRTENERIK SML
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1I9LRL0 | A0A1I9LRL0_ARATH | (S)-2-hydroxy-acid oxidase | HAOX1 At3g14130 | 319 | Annotation score: | ||
A0A1I9LRL2 | A0A1I9LRL2_ARATH | (S)-2-hydroxy-acid oxidase | HAOX1 At3g14130 | 255 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 65 | M → I in AAM61594 (Ref. 4) Curated | 1 | |
Sequence conflicti | 107 | S → P in AAM61594 (Ref. 4) Curated | 1 | |
Sequence conflicti | 273 | A → V in AAM61594 (Ref. 4) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP000600 Genomic DNA Translation: BAB02977.1 CP002686 Genomic DNA Translation: AEE75475.1 CP002686 Genomic DNA Translation: ANM65219.1 BT002739 mRNA Translation: AAO22568.1 AY085037 mRNA Translation: AAM61594.1 |
RefSeqi | NP_001327205.1, NM_001338103.1 NP_188029.1, NM_112269.3 |
Genome annotation databases
EnsemblPlantsi | AT3G14130.1; AT3G14130.1; AT3G14130 AT3G14130.3; AT3G14130.3; AT3G14130 |
GeneIDi | 820630 |
Gramenei | AT3G14130.1; AT3G14130.1; AT3G14130 AT3G14130.3; AT3G14130.3; AT3G14130 |
KEGGi | ath:AT3G14130 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP000600 Genomic DNA Translation: BAB02977.1 CP002686 Genomic DNA Translation: AEE75475.1 CP002686 Genomic DNA Translation: ANM65219.1 BT002739 mRNA Translation: AAO22568.1 AY085037 mRNA Translation: AAM61594.1 |
RefSeqi | NP_001327205.1, NM_001338103.1 NP_188029.1, NM_112269.3 |
3D structure databases
SMRi | Q9LJH5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT3G14130.1 |
Proteomic databases
PaxDbi | Q9LJH5 |
Genome annotation databases
EnsemblPlantsi | AT3G14130.1; AT3G14130.1; AT3G14130 AT3G14130.3; AT3G14130.3; AT3G14130 |
GeneIDi | 820630 |
Gramenei | AT3G14130.1; AT3G14130.1; AT3G14130 AT3G14130.3; AT3G14130.3; AT3G14130 |
KEGGi | ath:AT3G14130 |
Organism-specific databases
Araporti | AT3G14130 |
TAIRi | locus:2087487, AT3G14130 |
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
HOGENOMi | CLU_020639_6_1_1 |
InParanoidi | Q9LJH5 |
OMAi | FQYEIYL |
OrthoDBi | 879633at2759 |
PhylomeDBi | Q9LJH5 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
BioCyci | ARA:AT3G14130-MONOMER |
BRENDAi | 1.1.3.15, 399 |
Miscellaneous databases
PROi | PR:Q9LJH5 |
Gene expression databases
ExpressionAtlasi | Q9LJH5, baseline and differential |
Genevisiblei | Q9LJH5, AT |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HAOX1_ARATH | |
Accessioni | Q9LJH5Primary (citable) accession number: Q9LJH5 Secondary accession number(s): Q8LF60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2011 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families