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Protein

Plastidial pyruvate kinase 1, chloroplastic

Gene

PKP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination.2 Publications

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

pH dependencei

Optimum pH is 7.8-8.2.2 Publications

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
  2. Phosphoglycerate kinase 3, cytosolic (PGK3)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1), Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590)
  4. Enolase 1, chloroplastic (ENO1), Bifunctional enolase 2/transcriptional activator (ENO2), Cytosolic enolase 3 (ENO3)
  5. Plastidial pyruvate kinase 2 (PKP2), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Plastidial pyruvate kinase 4, chloroplastic (PKP4), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (At3g25960), Pyruvate kinase (At2g36580), Pyruvate kinase (MAH20.13), Pyruvate kinase (F1I16_220), Pyruvate kinase (At3g04050), Pyruvate kinase (F1I16_60), Pyruvate kinase (MCD7.8), Pyruvate kinase (At3g52990), Pyruvate kinase (MBK5.16), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At1g33230), Pyruvate kinase, Pyruvate kinase (AXX17_At3g03460), Pyruvate kinase, Pyruvate kinase (AXX17_At3g50440), Pyruvate kinase (F8J2_160), Pyruvate kinase (AXX17_At5g51800), Pyruvate kinase (AXX17_At3g50280), Pyruvate kinase (AXX17_At2g33300), Pyruvate kinase (AXX17_At3g47400), Pyruvate kinase (At5g63680), Pyruvate kinase (AXX17_At4g30430), Pyruvate kinase (AXX17_At3g24740), Pyruvate kinase (AXX17_At3g28070), Pyruvate kinase (AXX17_At5g55530), Pyruvate kinase (AXX17_ATUG04870)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147SubstrateBy similarity1
Metal bindingi149PotassiumBy similarity1
Metal bindingi181PotassiumBy similarity1
Metal bindingi182Potassium; via carbonyl oxygenBy similarity1
Binding sitei344Substrate; via amide nitrogenBy similarity1
Sitei344Transition state stabilizerBy similarity1
Metal bindingi346MagnesiumBy similarity1
Binding sitei369Substrate; via amide nitrogenBy similarity1
Metal bindingi370MagnesiumBy similarity1
Binding sitei370Substrate; via amide nitrogenBy similarity1
Binding sitei402SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to insulin stimulus Source: GO_Central
  • glycolytic process Source: GO_Central
  • lipid metabolic process Source: TAIR
  • response to cadmium ion Source: TAIR
  • seed maturation Source: UniProtKB

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKiQ9LIK0
UniPathwayi
UPA00109;UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Plastidial pyruvate kinase 1, chloroplastic (EC:2.7.1.40)
Short name:
PK1
Short name:
PKp1
Alternative name(s):
Pyruvate kinase II
Pyruvate kinase isozyme A
Short name:
PKP-ALPHA
Gene namesi
Name:PKP1
Synonyms:PK1
Ordered Locus Names:At3g22960
ORF Names:F5N5.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G22960
TAIRilocus:2084583 AT3G22960

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 87ChloroplastBy similarityAdd BLAST87
ChainiPRO_000041698788 – 596Plastidial pyruvate kinase 1, chloroplasticAdd BLAST509

Proteomic databases

PaxDbiQ9LIK0
PRIDEiQ9LIK0

PTM databases

iPTMnetiQ9LIK0

Expressioni

Tissue specificityi

Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), stems, inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals).2 Publications

Developmental stagei

In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips.1 Publication

Gene expression databases

ExpressionAtlasiQ9LIK0 baseline and differential
GenevisibleiQ9LIK0 AT

Interactioni

Subunit structurei

Oligomer of alpha and beta subunits.1 Publication

Protein-protein interaction databases

BioGridi7202, 30 interactors
IntActiQ9LIK0, 30 interactors
STRINGi3702.AT3G22960.1

Structurei

3D structure databases

ProteinModelPortaliQ9LIK0
SMRiQ9LIK0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2323 Eukaryota
COG0469 LUCA
HOGENOMiHOG000021559
InParanoidiQ9LIK0
KOiK00873
OMAiGQFPEKA
OrthoDBiEOG0936061N
PhylomeDBiQ9LIK0

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 1 hit
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LIK0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQSIQFSTP SHTPHLLHLP HSQFNRPLSS ISFRRFPLTT IKYTSIRASS
60 70 80 90 100
SSSPSPDLDS SSSSSSSQVL LSPNGTGAVK SDERSVVATA VTTDTSGIEV
110 120 130 140 150
DTVTEAELKE NGFRSTRRTK LICTIGPATC GFEQLEALAV GGMNVARLNM
160 170 180 190 200
CHGTRDWHRG VIRSVRRLNE EKGFAVAIMM DTEGSEIHMG DLGGEASAKA
210 220 230 240 250
EDGEVWTFTV RAFDSSRPER TISVSYDGFA EDVRVGDELL VDGGMVRFEV
260 270 280 290 300
IEKIGPDVKC LCTDPGLLLP RANLTFWRDG SLVRERNAML PTISSKDWLD
310 320 330 340 350
IDFGIAEGVD FIAVSFVKSA EVINHLKSYL AARSRGGEIG VIAKIESIDS
360 370 380 390 400
LTNLEEIILA SDGAMVARGD LGAQIPLEQV PAAQQRIVQV CRALNKPVIV
410 420 430 440 450
ASQLLESMIE YPTPTRAEVA DVSEAVRQRS DALMLSGESA MGQFPDKALT
460 470 480 490 500
VLRTVSLRIE RWWREEKRHE SVPLQAIGSS FSDKISEEIC NSAAKMANNL
510 520 530 540 550
GVDAVFVYTT SGHMASLVSR CRPDCPIFAF TTTTSVRRRL NLQWGLIPFR
560 570 580 590
LSFSDDMESN LNKTFSLLKS RGMIKSGDLV IAVSDMLQSI QVMNVP
Length:596
Mass (Da):65,131
Last modified:October 1, 2000 - v1
Checksum:i256CE329639A4BFD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9T → S in AAM61702 (Ref. 4) Curated1
Sequence conflicti52S → P in AAM61702 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001300 Genomic DNA Translation: BAB03043.1
CP002686 Genomic DNA Translation: AEE76697.1
AY056196 mRNA Translation: AAL07045.2
AY056793 mRNA Translation: AAL10484.1
AY058084 mRNA Translation: AAL24192.1
BT002329 mRNA Translation: AAN86162.1
AY085149 mRNA Translation: AAM61702.1
RefSeqiNP_566720.1, NM_113196.3
UniGeneiAt.5909
At.74807
At.75004

Genome annotation databases

EnsemblPlantsiAT3G22960.1; AT3G22960.1; AT3G22960
GeneIDi821870
GrameneiAT3G22960.1; AT3G22960.1; AT3G22960
KEGGiath:AT3G22960

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001300 Genomic DNA Translation: BAB03043.1
CP002686 Genomic DNA Translation: AEE76697.1
AY056196 mRNA Translation: AAL07045.2
AY056793 mRNA Translation: AAL10484.1
AY058084 mRNA Translation: AAL24192.1
BT002329 mRNA Translation: AAN86162.1
AY085149 mRNA Translation: AAM61702.1
RefSeqiNP_566720.1, NM_113196.3
UniGeneiAt.5909
At.74807
At.75004

3D structure databases

ProteinModelPortaliQ9LIK0
SMRiQ9LIK0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7202, 30 interactors
IntActiQ9LIK0, 30 interactors
STRINGi3702.AT3G22960.1

PTM databases

iPTMnetiQ9LIK0

Proteomic databases

PaxDbiQ9LIK0
PRIDEiQ9LIK0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G22960.1; AT3G22960.1; AT3G22960
GeneIDi821870
GrameneiAT3G22960.1; AT3G22960.1; AT3G22960
KEGGiath:AT3G22960

Organism-specific databases

AraportiAT3G22960
TAIRilocus:2084583 AT3G22960

Phylogenomic databases

eggNOGiKOG2323 Eukaryota
COG0469 LUCA
HOGENOMiHOG000021559
InParanoidiQ9LIK0
KOiK00873
OMAiGQFPEKA
OrthoDBiEOG0936061N
PhylomeDBiQ9LIK0

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00188

SABIO-RKiQ9LIK0

Miscellaneous databases

PROiPR:Q9LIK0

Gene expression databases

ExpressionAtlasiQ9LIK0 baseline and differential
GenevisibleiQ9LIK0 AT

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 1 hit
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPKP1_ARATH
AccessioniPrimary (citable) accession number: Q9LIK0
Secondary accession number(s): Q8LEY6, Q93ZY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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