UniProtKB - Q9LHQ7 (MCES1_ARATH)
Protein
mRNA cap guanine-N7 methyltransferase 1
Gene
At3g20650
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC (By similarity).By similarity
Catalytic activityi
- a 5'-end (5'-triphosphoguanosine)-guanosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-guanosine in mRNA + S-adenosyl-L-homocysteinePROSITE-ProRule annotationEC:2.1.1.56PROSITE-ProRule annotation
- a 5'-end (5'-triphosphoguanosine)-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-adenosine in mRNA + S-adenosyl-L-homocysteinePROSITE-ProRule annotationEC:2.1.1.56PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 74 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 92 | S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Sitei | 95 | mRNA cap bindingPROSITE-ProRule annotation | 1 | |
Sitei | 101 | mRNA cap bindingPROSITE-ProRule annotation | 1 | |
Binding sitei | 114 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Sitei | 126 | mRNA cap bindingPROSITE-ProRule annotation | 1 | |
Sitei | 176 | mRNA cap bindingPROSITE-ProRule annotation | 1 | |
Sitei | 264 | mRNA cap bindingPROSITE-ProRule annotation | 1 | |
Sitei | 333 | mRNA cap bindingPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- mRNA (guanine-N7-)-methyltransferase activity Source: GO_Central
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- 7-methylguanosine mRNA capping Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | mRNA capping, mRNA processing |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: mRNA cap guanine-N7 methyltransferase 1 (EC:2.1.1.56)Alternative name(s): mRNA (guanine-N(7)-)-methyltransferase 1 mRNA cap methyltransferase 1 |
Gene namesi | Ordered Locus Names:At3g20650 ORF Names:F3H11.3 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G20650 |
TAIRi | locus:2083420 AT3G20650 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleus Source: GO_Central
Other locations
- mRNA cap binding complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000248331 | 1 – 370 | mRNA cap guanine-N7 methyltransferase 1Add BLAST | 370 |
Proteomic databases
PaxDbi | Q9LHQ7 |
PRIDEi | Q9LHQ7 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9LHQ7 baseline and differential |
Genevisiblei | Q9LHQ7 AT |
Interactioni
Protein-protein interaction databases
BioGridi | 6944, 4 interactors |
STRINGi | 3702.AT3G20650.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 347 | mRNA cap 0 methyltransferasePROSITE-ProRule annotationAdd BLAST | 326 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 70 – 71 | mRNA cap bindingPROSITE-ProRule annotation | 2 | |
Regioni | 150 – 151 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 2 | |
Regioni | 172 – 174 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 3 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG1975 Eukaryota ENOG410Y7HG LUCA |
HOGENOMi | HOG000242614 |
InParanoidi | Q9LHQ7 |
KOi | K00565 |
OrthoDBi | 1390749at2759 |
PhylomeDBi | Q9LHQ7 |
Family and domain databases
InterProi | View protein in InterPro IPR004971 mRNA_G-N7_MeTrfase_dom IPR016899 mRNA_G-N7_MeTrfase_euk IPR039753 RG7MT1 IPR029063 SAM-dependent_MTases |
PANTHERi | PTHR12189 PTHR12189, 1 hit |
Pfami | View protein in Pfam PF03291 Pox_MCEL, 1 hit |
PIRSFi | PIRSF028762 ABD1, 1 hit |
SUPFAMi | SSF53335 SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51562 RNA_CAP0_MT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9LHQ7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKRGFSDSPS SSAPPPSSRF KSNPEGDSQF LEDETTKNFA RKVADHYSRR
60 70 80 90 100
TNQTLEEREA SPIIHLKKLN NWIKSVLIQL YARPDDAVLD LACGKGGDLI
110 120 130 140 150
KWDKARIGYY VGIDIAEGSI EDCRTRYNGD ADHHQRRKKF SFPSRLLCGD
160 170 180 190 200
CFEVELDKIL EEDAPFDICS CQFAMHYSWT TEARARRALA NVSALLRPGG
210 220 230 240 250
VFIGTMPDAN VIIKKLREAE GLEIGNSVYW IRFGEEYSQK KFKSSSPFGI
260 270 280 290 300
EYVFHLEDAV DCPEWIVPFN VFKSLAEEYD LELVFVKNSH EFVHEYMKKP
310 320 330 340 350
EFVELMRRLG ALGDGSNDQS TLSADEWEAA YLYLSFVLRK RGESDGARRS
360 370
GRRKNGKMNL SKDDVLYIDS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4JER8 | F4JER8_ARATH | mRNA cap guanine-N7 methyltransfera... | At3g20650 | 369 | Annotation score: | ||
A0A1I9LQA7 | A0A1I9LQA7_ARATH | mRNA cap guanine-N7 methyltransfera... | At3g20650 | 357 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP002034 Genomic DNA Translation: BAB02241.1 CP002686 Genomic DNA Translation: AEE76407.1 BT005804 mRNA Translation: AAO64206.1 BT006067 mRNA Translation: AAP04052.1 AK228381 mRNA Translation: BAF00319.1 |
RefSeqi | NP_188701.2, NM_112956.6 [Q9LHQ7-1] |
Genome annotation databases
EnsemblPlantsi | AT3G20650.1; AT3G20650.1; AT3G20650 [Q9LHQ7-1] |
GeneIDi | 821612 |
Gramenei | AT3G20650.1; AT3G20650.1; AT3G20650 [Q9LHQ7-1] |
KEGGi | ath:AT3G20650 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP002034 Genomic DNA Translation: BAB02241.1 CP002686 Genomic DNA Translation: AEE76407.1 BT005804 mRNA Translation: AAO64206.1 BT006067 mRNA Translation: AAP04052.1 AK228381 mRNA Translation: BAF00319.1 |
RefSeqi | NP_188701.2, NM_112956.6 [Q9LHQ7-1] |
3D structure databases
SMRi | Q9LHQ7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 6944, 4 interactors |
STRINGi | 3702.AT3G20650.1 |
Proteomic databases
PaxDbi | Q9LHQ7 |
PRIDEi | Q9LHQ7 |
Genome annotation databases
EnsemblPlantsi | AT3G20650.1; AT3G20650.1; AT3G20650 [Q9LHQ7-1] |
GeneIDi | 821612 |
Gramenei | AT3G20650.1; AT3G20650.1; AT3G20650 [Q9LHQ7-1] |
KEGGi | ath:AT3G20650 |
Organism-specific databases
Araporti | AT3G20650 |
TAIRi | locus:2083420 AT3G20650 |
Phylogenomic databases
eggNOGi | KOG1975 Eukaryota ENOG410Y7HG LUCA |
HOGENOMi | HOG000242614 |
InParanoidi | Q9LHQ7 |
KOi | K00565 |
OrthoDBi | 1390749at2759 |
PhylomeDBi | Q9LHQ7 |
Miscellaneous databases
PROi | PR:Q9LHQ7 |
Gene expression databases
ExpressionAtlasi | Q9LHQ7 baseline and differential |
Genevisiblei | Q9LHQ7 AT |
Family and domain databases
InterProi | View protein in InterPro IPR004971 mRNA_G-N7_MeTrfase_dom IPR016899 mRNA_G-N7_MeTrfase_euk IPR039753 RG7MT1 IPR029063 SAM-dependent_MTases |
PANTHERi | PTHR12189 PTHR12189, 1 hit |
Pfami | View protein in Pfam PF03291 Pox_MCEL, 1 hit |
PIRSFi | PIRSF028762 ABD1, 1 hit |
SUPFAMi | SSF53335 SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51562 RNA_CAP0_MT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCES1_ARATH | |
Accessioni | Q9LHQ7Primary (citable) accession number: Q9LHQ7 Secondary accession number(s): Q3EB33 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2006 |
Last sequence update: | October 1, 2000 | |
Last modified: | September 18, 2019 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names