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Protein

Probable mediator of RNA polymerase II transcription subunit 37c

Gene

MED37C

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity
ATP-dependent molecular chaperone that assists folding of unfolded or misfolded proteins under stress conditions. Mediates plastid precursor degradation to prevent cytosolic precursor accumulation, together with the E3 ubiquitin-protein ligase CHIP. Recognizes specific sequence motifs in transit peptides and thereby led to precursor degradation through the ubiquitin-proteasome system. Plays a critical role in embryogenesis.1 Publication
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functioni

  • ATP binding Source: TAIR
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • protein ubiquitination Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to bacterium Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to heat Source: UniProtKB
  • response to high light intensity Source: TAIR
  • response to hydrogen peroxide Source: TAIR
  • response to temperature stimulus Source: TAIR
  • response to virus Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChaperone
Biological processStress response, Transcription, Transcription regulation, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371453 Regulation of HSF1-mediated heat shock response
R-ATH-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-ATH-3371571 HSF1-dependent transactivation
R-ATH-6798695 Neutrophil degranulation
R-ATH-72163 mRNA Splicing - Major Pathway
R-ATH-8876725 Protein methylation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mediator of RNA polymerase II transcription subunit 37c
Alternative name(s):
Heat shock 70 kDa protein 4
Heat shock cognate 70 kDa protein 4
Heat shock cognate protein 70-4
Short name:
AtHsc70-4
Heat shock protein 70-4
Short name:
AtHsp70-4
Gene namesi
Name:MED37C
Synonyms:HSC70-4, HSP70, HSP70-4, MED37_2
Ordered Locus Names:At3g12580
ORF Names:T16H11.7, T2E22.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G12580
TAIRilocus:2101222 AT3G12580

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Abnormal embryogenesis. Defective seedlings with high levels of reactive oxygen species and monoubiquitinated LHCB4 precursors.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003970451 – 650Probable mediator of RNA polymerase II transcription subunit 37cAdd BLAST650

Proteomic databases

PaxDbiQ9LHA8
PRIDEiQ9LHA8

PTM databases

iPTMnetiQ9LHA8

Expressioni

Developmental stagei

Up-regulated during seed maturation.1 Publication

Inductioni

By heat shock, cold, high light treatment and oxidative stress. Up-regulated by virus infection and by pathogen attack (P.syringae).4 Publications

Gene expression databases

ExpressionAtlasiQ9LHA8 baseline and differential
GenevisibleiQ9LHA8 AT

Interactioni

Subunit structurei

Component of the Mediator complex (Probable). Interacts with CHIP. Interacts with HSFA1A/HSF1.Curated3 Publications

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi5772, 22 interactors
IntActiQ9LHA8, 8 interactors
MINTiQ9LHA8
STRINGi3702.AT3G12580.1

Structurei

3D structure databases

ProteinModelPortaliQ9LHA8
SMRiQ9LHA8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0101 Eukaryota
COG0443 LUCA
HOGENOMiHOG000228135
InParanoidiQ9LHA8
KOiK03283
OMAiQRKHKRD
OrthoDBiEOG093604KP
PhylomeDBiQ9LHA8

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LHA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKGEGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS
60 70 80 90 100
ERLIGDAAKN QVAMNPTNTV FDAKRLIGRR YSDPSVQADK SHWPFKVVSG
110 120 130 140 150
PGEKPMIVVN HKGEEKQFSA EEISSMVLIK MREIAEAFLG SPVKNAVVTV
160 170 180 190 200
PAYFNDSQRQ ATKDAGVISG LNVMRIINEP TAAAIAYGLD KKASSVGEKN
210 220 230 240 250
VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE
260 270 280 290 300
FKRKNKKDIT GNPRALRRLR TACERAKRTL SSTAQTTIEI DSLFEGIDFY
310 320 330 340 350
TTITRARFEE LNMDLFRKCM EPVEKCLRDA KMDKSSVHDV VLVGGSTRIP
360 370 380 390 400
KVQQLLQDFF NGKELCKSIN PDEAVAYGAA VQAAILSGEG NEKVQDLLLL
410 420 430 440 450
DVTPLSLGLE TAGGVMTVLI PRNTTIPTKK EQIFSTYSDN QPGVLIQVYE
460 470 480 490 500
GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT
510 520 530 540 550
TGQKNKITIT NDKGRLSKEE IEKMVQEAEK YKAEDEEHKK KVDAKNALEN
560 570 580 590 600
YAYNMRNTIK DEKIASKLDA ADKKKIEDAI DQAIEWLDGN QLAEADEFED
610 620 630 640 650
KMKELESLCN PIIARMYQGA GPDMGGAGGM DDDTPAGGSG GAGPKIEEVD
Length:650
Mass (Da):71,101
Last modified:October 1, 2000 - v1
Checksum:i33381E896461C5B9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126M → I in CAA05547 (Ref. 1) Curated1
Sequence conflicti149T → I in CAA05547 (Ref. 1) Curated1
Sequence conflicti161A → G in CAA05547 (Ref. 1) Curated1
Sequence conflicti342L → V in CAA05547 (Ref. 1) Curated1
Sequence conflicti356L → V in CAA05547 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002551 mRNA Translation: CAA05547.1
AP002055 Genomic DNA Translation: BAB02269.1
AC069474 Genomic DNA Translation: AAG51030.1
CP002686 Genomic DNA Translation: AEE75218.1
AY054183 mRNA Translation: AAL06844.1
AY054190 mRNA Translation: AAL06851.1
AY059885 mRNA Translation: AAL24367.1
PIRiJA0171
RefSeqiNP_187864.1, NM_112093.3
UniGeneiAt.47608

Genome annotation databases

EnsemblPlantsiAT3G12580.1; AT3G12580.1; AT3G12580
GeneIDi820438
GrameneiAT3G12580.1; AT3G12580.1; AT3G12580
KEGGiath:AT3G12580

Similar proteinsi

Entry informationi

Entry nameiMD37C_ARATH
AccessioniPrimary (citable) accession number: Q9LHA8
Secondary accession number(s): Q9ZS55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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