MED37C

Functionⁱ

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

ATP-dependent molecular chaperone that assists folding of unfolded or misfolded proteins under stress conditions. Mediates plastid precursor degradation to prevent cytosolic precursor accumulation, together with the E3 ubiquitin-protein ligase CHIP. Recognizes specific sequence motifs in transit peptides and thereby led to precursor degradation through the ubiquitin-proteasome system. Plays a critical role in embryogenesis.1 Publication

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functionⁱ

ATPase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
ATPase activity, coupled
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
ATP binding
Source: TAIR
Inferred from direct assayⁱ
- 17137349
heat shock protein binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
misfolded protein binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein binding involved in protein folding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
ubiquitin protein ligase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 20028838
unfolded protein binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to heat
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
cellular response to unfolded protein
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
chaperone cofactor-dependent protein refolding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein refolding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein ubiquitination
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 20028838
response to bacterium
Source: TAIR
Inferred from expression patternⁱ
- 18065690
response to cadmium ion
Source: TAIR
Inferred from expression patternⁱ
- 16502469
response to heat
Source: UniProtKB
Inferred from expression patternⁱ
- 15805473
response to high light intensity
Source: TAIR
Inferred from expression patternⁱ
- 17059409
response to hydrogen peroxide
Source: TAIR
Inferred from expression patternⁱ
- 17059409
response to temperature stimulus
Source: TAIR
Inferred from expression patternⁱ
- 20079334
response to unfolded protein
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
response to virus
Source: TAIR
Inferred from expression patternⁱ
- 15805473

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chaperone
Biological process	Stress response, Transcription, Transcription regulation, Ubl conjugation pathway
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-ATH-3371453 Regulation of HSF1-mediated heat shock response R-ATH-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR) R-ATH-3371571 HSF1-dependent transactivation R-ATH-6798695 Neutrophil degranulation R-ATH-72163 mRNA Splicing - Major Pathway R-ATH-8876725 Protein methylation

Reactomeⁱ

R-ATH-3371453 Regulation of HSF1-mediated heat shock response
R-ATH-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-ATH-3371571 HSF1-dependent transactivation
R-ATH-6798695 Neutrophil degranulation
R-ATH-72163 mRNA Splicing - Major Pathway
R-ATH-8876725 Protein methylation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable mediator of RNA polymerase II transcription subunit 37c Alternative name(s): Heat shock 70 kDa protein 4 Heat shock cognate 70 kDa protein 4 Heat shock cognate protein 70-4 Short name: AtHsc70-4 Heat shock protein 70-4 Short name: AtHsp70-4
Gene namesⁱ	Name:MED37C Synonyms:HSC70-4, HSP70, HSP70-4, MED37_2 Ordered Locus Names:At3g12580 ORF Names:T16H11.7, T2E22.11
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 3

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT3G12580
TAIRⁱ	locus:2101222 AT3G12580

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Abnormal embryogenesis. Defective seedlings with high levels of reactive oxygen species and monoubiquitinated LHCB4 precursors.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000397045	1 – 650	Probable mediator of RNA polymerase II transcription subunit 37cAdd BLAST		650

Proteomic databases

PaxDbⁱ	Q9LHA8
PRIDEⁱ	Q9LHA8

PTM databases

iPTMnetⁱ	Q9LHA8

iPTMnetⁱ

Q9LHA8

Expressionⁱ

Developmental stageⁱ

Up-regulated during seed maturation.1 Publication

Inductionⁱ

By heat shock, cold, high light treatment and oxidative stress. Up-regulated by virus infection and by pathogen attack (P.syringae).4 Publications

Gene expression databases

ExpressionAtlasⁱ	Q9LHA8 baseline and differential
Genevisibleⁱ	Q9LHA8 AT

Interactionⁱ

Subunit structureⁱ

Component of the Mediator complex (Probable). Interacts with CHIP. Interacts with HSFA1A/HSF1.Curated3 Publications

GO - Molecular functionⁱ

heat shock protein binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
misfolded protein binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein binding involved in protein folding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
ubiquitin protein ligase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 20028838
unfolded protein binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	5772, 22 interactors
IntActⁱ	Q9LHA8, 8 interactors
Molecular INTeraction database More...MINTⁱ	Q9LHA8
STRINGⁱ	3702.AT3G12580.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9LHA8
SMRⁱ	Q9LHA8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the heat shock protein 70 (TC 1.A.33) family. DnaK subfamily. [View classification]Curated

Phylogenomic databases

eggNOGⁱ	KOG0101 Eukaryota COG0443 LUCA
HOGENOMⁱ	HOG000228135
InParanoidⁱ	Q9LHA8
KEGG Orthology (KO) More...KOⁱ	K03283
OMAⁱ	QEVANPI
Database of Orthologous Groups More...OrthoDBⁱ	EOG093604KP
PhylomeDBⁱ	Q9LHA8

Family and domain databases

Gene3Dⁱ	1.20.1270.10, 1 hit 2.60.34.10, 1 hit
InterProⁱ	View protein in InterPro IPR018181 Heat_shock_70_CS IPR029048 HSP70_C_sf IPR029047 HSP70_peptide-bd_sf IPR013126 Hsp_70_fam
PANTHERⁱ	PTHR19375 PTHR19375, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00012 HSP70, 1 hit
PRINTSⁱ	PR00301 HEATSHOCK70
SUPFAMⁱ	SSF100920 SSF100920, 1 hit SSF100934 SSF100934, 1 hit
PROSITEⁱ	View protein in PROSITE PS00297 HSP70_1, 1 hit PS00329 HSP70_2, 1 hit PS01036 HSP70_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9LHA8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAGKGEGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS 
        60         70         80         90        100
ERLIGDAAKN QVAMNPTNTV FDAKRLIGRR YSDPSVQADK SHWPFKVVSG 
       110        120        130        140        150
PGEKPMIVVN HKGEEKQFSA EEISSMVLIK MREIAEAFLG SPVKNAVVTV 
       160        170        180        190        200
PAYFNDSQRQ ATKDAGVISG LNVMRIINEP TAAAIAYGLD KKASSVGEKN 
       210        220        230        240        250
VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE 
       260        270        280        290        300
FKRKNKKDIT GNPRALRRLR TACERAKRTL SSTAQTTIEI DSLFEGIDFY 
       310        320        330        340        350
TTITRARFEE LNMDLFRKCM EPVEKCLRDA KMDKSSVHDV VLVGGSTRIP 
       360        370        380        390        400
KVQQLLQDFF NGKELCKSIN PDEAVAYGAA VQAAILSGEG NEKVQDLLLL 
       410        420        430        440        450
DVTPLSLGLE TAGGVMTVLI PRNTTIPTKK EQIFSTYSDN QPGVLIQVYE 
       460        470        480        490        500
GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT 
       510        520        530        540        550
TGQKNKITIT NDKGRLSKEE IEKMVQEAEK YKAEDEEHKK KVDAKNALEN 
       560        570        580        590        600
YAYNMRNTIK DEKIASKLDA ADKKKIEDAI DQAIEWLDGN QLAEADEFED 
       610        620        630        640        650
KMKELESLCN PIIARMYQGA GPDMGGAGGM DDDTPAGGSG GAGPKIEEVD

Length:650

Mass (Da):71,101

Last modified:October 1, 2000 - v1

Checksum:ⁱ33381E896461C5B9

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	126	M → I in CAA05547 (Ref. 1) Curated	1
Sequence conflictⁱ	149	T → I in CAA05547 (Ref. 1) Curated	1
Sequence conflictⁱ	161	A → G in CAA05547 (Ref. 1) Curated	1
Sequence conflictⁱ	342	L → V in CAA05547 (Ref. 1) Curated	1
Sequence conflictⁱ	356	L → V in CAA05547 (Ref. 1) Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ002551 mRNA Translation: CAA05547.1 AP002055 Genomic DNA Translation: BAB02269.1 AC069474 Genomic DNA Translation: AAG51030.1 CP002686 Genomic DNA Translation: AEE75218.1 AY054183 mRNA Translation: AAL06844.1 AY054190 mRNA Translation: AAL06851.1 AY059885 mRNA Translation: AAL24367.1
PIRⁱ	JA0171
NCBI Reference Sequences More...RefSeqⁱ	NP_187864.1, NM_112093.3
UniGeneⁱ	At.47608

Genome annotation databases

EnsemblPlantsⁱ	AT3G12580.1; AT3G12580.1; AT3G12580
GeneIDⁱ	820438
Grameneⁱ	AT3G12580.1; AT3G12580.1; AT3G12580
KEGGⁱ	ath:AT3G12580

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9LHA8	HSP70		ARATH		650	UniRef100_Q9LHA8

Protein	Similar proteins		Species	Score	Length	Source
Q9LHA8	Probable mediator of RNA polymerase II transcription subunit 37e		ARATH		651	UniRef90_P22953
Chloroplast envelope membrane 70 kDa heat shock-related protein		SPIOL		653
Heat shock cognate 70 kDa protein		PETHY		651
Heat shock cognate 70 kDa protein 1		SOLLC		650
Heat shock 70 kDa protein		MAIZE		645
+778

Protein	Similar proteins		Species	Score	Length	Source
Q9LHA8	Heat shock 70 kDa protein 1A	)	HUMAN		641	UniRef50_P0DMV8
Heat shock 70 kDa protein 1		PONAB		641
Heat shock 70 kDa protein 1B		MOUSE		642
Heat shock 70 kDa protein 1B		BOVIN		641
Heat shock 70 kDa protein 1B		BOSMU		641
+2027

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ002551 mRNA Translation: CAA05547.1 AP002055 Genomic DNA Translation: BAB02269.1 AC069474 Genomic DNA Translation: AAG51030.1 CP002686 Genomic DNA Translation: AEE75218.1 AY054183 mRNA Translation: AAL06844.1 AY054190 mRNA Translation: AAL06851.1 AY059885 mRNA Translation: AAL24367.1
PIRⁱ	JA0171
RefSeqⁱ	NP_187864.1, NM_112093.3
UniGeneⁱ	At.47608

3D structure databases

ProteinModelPortalⁱ	Q9LHA8
SMRⁱ	Q9LHA8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	5772, 22 interactors
IntActⁱ	Q9LHA8, 8 interactors
MINTⁱ	Q9LHA8
STRINGⁱ	3702.AT3G12580.1

PTM databases

iPTMnetⁱ	Q9LHA8

iPTMnetⁱ

Q9LHA8

Proteomic databases

PaxDbⁱ	Q9LHA8
PRIDEⁱ	Q9LHA8

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT3G12580.1; AT3G12580.1; AT3G12580
GeneIDⁱ	820438
Grameneⁱ	AT3G12580.1; AT3G12580.1; AT3G12580
KEGGⁱ	ath:AT3G12580

Organism-specific databases

Araportⁱ	AT3G12580
TAIRⁱ	locus:2101222 AT3G12580

Phylogenomic databases

eggNOGⁱ	KOG0101 Eukaryota COG0443 LUCA
HOGENOMⁱ	HOG000228135
InParanoidⁱ	Q9LHA8
KOⁱ	K03283
OMAⁱ	QEVANPI
OrthoDBⁱ	EOG093604KP
PhylomeDBⁱ	Q9LHA8

Enzyme and pathway databases

Reactomeⁱ	R-ATH-3371453 Regulation of HSF1-mediated heat shock response R-ATH-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR) R-ATH-3371571 HSF1-dependent transactivation R-ATH-6798695 Neutrophil degranulation R-ATH-72163 mRNA Splicing - Major Pathway R-ATH-8876725 Protein methylation

Reactomeⁱ

R-ATH-3371453 Regulation of HSF1-mediated heat shock response
R-ATH-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-ATH-3371571 HSF1-dependent transactivation
R-ATH-6798695 Neutrophil degranulation
R-ATH-72163 mRNA Splicing - Major Pathway
R-ATH-8876725 Protein methylation

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9LHA8

PROⁱ

PR:Q9LHA8

Gene expression databases

ExpressionAtlasⁱ	Q9LHA8 baseline and differential
Genevisibleⁱ	Q9LHA8 AT

Family and domain databases

Gene3Dⁱ	1.20.1270.10, 1 hit 2.60.34.10, 1 hit
InterProⁱ	View protein in InterPro IPR018181 Heat_shock_70_CS IPR029048 HSP70_C_sf IPR029047 HSP70_peptide-bd_sf IPR013126 Hsp_70_fam
PANTHERⁱ	PTHR19375 PTHR19375, 1 hit
Pfamⁱ	View protein in Pfam PF00012 HSP70, 1 hit
PRINTSⁱ	PR00301 HEATSHOCK70
SUPFAMⁱ	SSF100920 SSF100920, 1 hit SSF100934 SSF100934, 1 hit
PROSITEⁱ	View protein in PROSITE PS00297 HSP70_1, 1 hit PS00329 HSP70_2, 1 hit PS01036 HSP70_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MD37C_ARATH
Accessionⁱ	Q9LHA8Primary (citable) accession number: Q9LHA8 Secondary accession number(s): Q9ZS55
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
	Last sequence update:	October 1, 2000
	Last modified:	November 7, 2018
	This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

UniProtKB

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Supporting data

UniProtKB - Q9LHA8 (MD37C_ARATH)

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Probable mediator of RNA polymerase II transcription subunit 37c

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cell Wall

Cytosol

Extracellular region or secreted

Golgi apparatus

Mitochondrion

Nucleus

Plasma Membrane

Vacuole

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ