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Protein

Fructose-bisphosphate aldolase 8, cytosolic

Gene

FBA8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Fructose-bisphosphate aldolase that plays a key role in glycolysis and gluconeogenesis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Curated
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (MJB21.12), Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (FBA6), Fructose-bisphosphate aldolase (FBA4), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 6, cytosolic (FBA6), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39SubstrateBy similarity1
Active sitei183Proton acceptorBy similarity1
Active sitei225Schiff-base intermediate with dihydroxyacetone-PBy similarity1
Binding sitei298SubstrateBy similarity1
Sitei358Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity1

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • fructose-bisphosphate aldolase activity Source: UniProtKB

GO - Biological processi

  • gluconeogenesis Source: UniProtKB
  • glycolytic process Source: UniProtKB
  • response to cadmium ion Source: TAIR
  • response to karrikin Source: TAIR
  • response to salt stress Source: TAIR

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandSchiff base

Enzyme and pathway databases

BioCyciARA:AT3G52930-MONOMER
ReactomeiR-ATH-114608 Platelet degranulation
R-ATH-6798695 Neutrophil degranulation
R-ATH-70171 Glycolysis
R-ATH-70263 Gluconeogenesis
R-ATH-70350 Fructose catabolism
UniPathwayiUPA00109; UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 8, cytosolicCurated (EC:4.1.2.13By similarity)
Short name:
AtFBA81 Publication
Alternative name(s):
Cytosolic aldolase 1Curated
Short name:
cAld1Curated
Gene namesi
Name:FBA8
Ordered Locus Names:At3g52930Imported
ORF Names:F8J2_100Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G52930
TAIRilocus:2085141 AT3G52930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Sterility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00004372412 – 358Fructose-bisphosphate aldolase 8, cytosolicAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei68S-glutathionyl cysteine; transientBy similarity1
Modified residuei173S-glutathionyl cysteine; transient; alternateBy similarity1
Modified residuei173S-nitrosocysteine; transient; alternateBy similarity1
Modified residuei350PhosphoserineCombined sources1
Modified residuei354N6,N6,N6-trimethyllysineBy similarity1

Post-translational modificationi

S-glutathionylated at Cys-68 and Cys-173.By similarity
S-nitrosylated at Cys-173.By similarity

Keywords - PTMi

Acetylation, Glutathionylation, Methylation, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiQ9LF98
PRIDEiQ9LF98
ProMEXiQ9LF98

PTM databases

iPTMnetiQ9LF98

Expressioni

Tissue specificityi

Highly expressed in flowers.1 Publication

Inductioni

By glucose, fructose and sucrose (PubMed:22561114). Induced by abiotic stresses (PubMed:22561114).1 Publication

Gene expression databases

ExpressionAtlasiQ9LF98 differential

Interactioni

Subunit structurei

Homotetramer (By similarity). Interacts with TRX3 (PubMed:15352244).By similarity1 Publication

Protein-protein interaction databases

IntActiQ9LF98, 4 interactors
STRINGi3702.AT3G52930.1

Structurei

3D structure databases

ProteinModelPortaliQ9LF98
SMRiQ9LF98
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni266 – 268Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1557 Eukaryota
COG3588 LUCA
HOGENOMiHOG000220876
KOiK01623
OMAiMILKPNM
OrthoDBiEOG09360ENE
PhylomeDBiQ9LF98

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR029768 Aldolase_I_AS
IPR013785 Aldolase_TIM
IPR000741 FBA_I
PfamiView protein in Pfam
PF00274 Glycolytic, 1 hit
PROSITEiView protein in PROSITE
PS00158 ALDOLASE_CLASS_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LF98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFTSKFAD ELIANAAYIG TPGKGILAAD ESTGTIGKRL ASINVENVET
60 70 80 90 100
NRRNLRELLF TAPGALPCLS GVILFEETLY QKSSDGKLFV DILKEGGVLP
110 120 130 140 150
GIKVDKGTVE LAGTDGETTT QGLDGLGDRC KKYYEAGARF AKWRAVLKIG
160 170 180 190 200
ENEPSEHSIH ENAYGLARYA VICQENGLVP IVEPEILVDG SHDIQKCAAV
210 220 230 240 250
TERVLAACYK ALSDHHVLLE GTLLKPNMVT PGSDSPKVSP EVIAEHTVRA
260 270 280 290 300
LQRTVPAAVP AIVFLSGGQS EEEATRNLNA MNQLKTKKPW SLSFSFGRAL
310 320 330 340 350
QQSTLKTWAG KEENVKAAQE ALYVRCKANS EATLGTYKGD AKLGDGAAES

LHVKDYKY
Length:358
Mass (Da):38,540
Last modified:October 1, 2000 - v1
Checksum:i0E25995B2EE0A319
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132969 Genomic DNA Translation: CAB86897.1
CP002686 Genomic DNA Translation: AEE79012.1
AY052709 mRNA Translation: AAK96613.1
AY057656 mRNA Translation: AAL15287.1
AY063718 mRNA Translation: AAL36068.1
AK317380 mRNA Translation: BAH20051.1
AY087346 mRNA Translation: AAM64896.1
PIRiT47550
RefSeqiNP_190861.1, NM_115153.4
UniGeneiAt.25299
At.75318

Genome annotation databases

EnsemblPlantsiAT3G52930.1; AT3G52930.1; AT3G52930
GeneIDi824459
GrameneiAT3G52930.1; AT3G52930.1; AT3G52930
KEGGiath:AT3G52930

Similar proteinsi

Entry informationi

Entry nameiALFC8_ARATH
AccessioniPrimary (citable) accession number: Q9LF98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2016
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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