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Protein

Ubiquitin carboxyl-terminal hydrolase 22

Gene

UBP22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei186NucleophilePROSITE-ProRule annotation1
Active sitei491Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri54 – 113UBP-typePROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiC19.A11

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 22 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 22
Short name:
AtUBP22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22
Gene namesi
Name:UBP22
Ordered Locus Names:At5g10790
ORF Names:T30N20_60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G10790
TAIRilocus:2183760 AT5G10790

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003130481 – 557Ubiquitin carboxyl-terminal hydrolase 22Add BLAST557

Proteomic databases

PaxDbiQ9LEW0
PRIDEiQ9LEW0

Expressioni

Gene expression databases

ExpressionAtlasiQ9LEW0 baseline and differential
GenevisibleiQ9LEW0 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G10790.1

Structurei

3D structure databases

ProteinModelPortaliQ9LEW0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini177 – 531USPAdd BLAST355

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri54 – 113UBP-typePROSITE-ProRule annotationAdd BLAST60

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1867 Eukaryota
ENOG410XQQ0 LUCA
HOGENOMiHOG000007260
InParanoidiQ9LEW0
KOiK11366
OMAiTEKHIHE
OrthoDBiEOG093608U4
PhylomeDBiQ9LEW0

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
IPR013083 Znf_RING/FYVE/PHD
IPR001607 Znf_UBP
PfamiView protein in Pfam
PF00443 UCH, 1 hit
PF02148 zf-UBP, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS50235 USP_3, 1 hit
PS50271 ZF_UBP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LEW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARISFLKN PDPCNHLSDY KLRYGTDGYK SFNNLFRCFN DARIKIKLQG
60 70 80 90 100
IDIPRCSYCS VYQKRLYICL ICRSISCSSH ILLHTQLNKG HDIAIDVERS
110 120 130 140 150
ELYCCACIDQ VYDSEFDEVV VSKQLFGLGM SVKSGADVVA VRSNKKRRLD
160 170 180 190 200
SQLIIGSNFL VSPRDRREKW TFPLGLRGLN NLGSTCFMNA VLQALVHAPP
210 220 230 240 250
LRNFWLSGQH NRDLCPRRTM GLLCLPCDLD VIFSAMFSGD RTPYSPAHLL
260 270 280 290 300
YSWWQHSTNL ATYEQQDSHE FFISLLDRIH ENEGKSKCLY QDNEECQCIT
310 320 330 340 350
HKAFSGLLRS DVTCTTCGST STTYDPFIDI SLTLDSMNGF SPADCRKNRY
360 370 380 390 400
SGGPSVNAIM PTLSGCLDFF TRSEKLGPDQ KLNCQSCGEK RESSKQMSIR
410 420 430 440 450
RLPLLLCLHV KRFEHSLTRK TSRKIDSYLQ YPFRLNMSPY LSSSIIGKRF
460 470 480 490 500
GNRIFAFDGE GEYDSSSSSS PSAEFEIFAV VTHKGMLESG HYVTYLRLKG
510 520 530 540 550
LWYRCDDAWI NEVEEEVVRG CECYMLFYAQ ETVIQKAHKE LSYQVISMAD

AFPFADC
Length:557
Mass (Da):63,518
Last modified:October 1, 2000 - v1
Checksum:i3CA6C313AF0FF82C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111V → A in AAG42760 (PubMed:11115897).Curated1
Sequence conflicti231V → I in AAG42760 (PubMed:11115897).Curated1
Sequence conflicti416S → F in AAG42760 (PubMed:11115897).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302670 mRNA Translation: AAG42760.1
AL365234 Genomic DNA Translation: CAB96834.1
CP002688 Genomic DNA Translation: AED91597.1
PIRiT50788
RefSeqiNP_568239.1, NM_121117.2
UniGeneiAt.6008

Genome annotation databases

EnsemblPlantsiAT5G10790.1; AT5G10790.1; AT5G10790
GeneIDi830946
GrameneiAT5G10790.1; AT5G10790.1; AT5G10790
KEGGiath:AT5G10790

Similar proteinsi

Entry informationi

Entry nameiUBP22_ARATH
AccessioniPrimary (citable) accession number: Q9LEW0
Secondary accession number(s): Q9FPS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2000
Last modified: June 20, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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