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UniProtKB - Q9LEL6 (6OMT_COPJA)
Protein
(RS)-norcoclaurine 6-O-methyltransferase
Gene
N/A
Organism
Coptis japonica (Japanese goldthread)
Status
Functioni
Catalyzes the transfer of the S-methyl group of S-adenosyl-L-methionine (AdoMet) to the 6-hydroxyl group of norcoclaurine to form coclaurine.
1 PublicationCatalytic activityi
- EC:2.1.1.1281 Publication
: (S)-reticuline biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-reticuline from (S)-norcoclaurine. This subpathway is part of the pathway (S)-reticuline biosynthesis, which is itself part of Alkaloid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-reticuline from (S)-norcoclaurine, the pathway (S)-reticuline biosynthesis and in Alkaloid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 192 | S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Binding sitei | 215 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 235 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 236 | S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation | 1 | |
Binding sitei | 249 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Active sitei | 253 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- (RS)-norcoclaurine 6-O-methyltransferase activity Source: UniProtKB-EC
- O-methyltransferase activity Source: InterPro
- protein dimerization activity Source: InterPro
GO - Biological processi
- methylation Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.128, 1610 |
SABIO-RKi | Q9LEL6 |
UniPathwayi | UPA00306;UER00441 |
Names & Taxonomyi
Protein namesi | Recommended name: (RS)-norcoclaurine 6-O-methyltransferase (EC:2.1.1.128)Alternative name(s): S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase Short name: 6-OMT |
Organismi | Coptis japonica (Japanese goldthread) |
Taxonomic identifieri | 3442 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Ranunculales › Ranunculaceae › Coptidoideae › Coptis |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204433 | 1 – 347 | (RS)-norcoclaurine 6-O-methyltransferaseAdd BLAST | 347 |
Interactioni
Subunit structurei
Homodimer.
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Family & Domainsi
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.PROSITE-ProRule annotation
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR012967, Plant_MeTrfase_dimerisation IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11746, PTHR11746, 1 hit |
Pfami | View protein in Pfam PF08100, Dimerisation, 1 hit PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9LEL6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEVKKDNLSS QAKLWNFIYG FAESLVLKCA VQLDLANIIH NSGTSMTLSE
60 70 80 90 100
LSSRLPSQPV NEDALYRVMR YLVHMKLFTK ASIDGELRYG LAPPAKYLVK
110 120 130 140 150
GWDKCMVGSI LAITDKDFMA PWHYLKDGLS GESGTAFEKA LGTNIWGYMA
160 170 180 190 200
EHPEKNQLFN EAMANDSRLI MSALVKECGN IFNGITTLVD VGGGTGTAVR
210 220 230 240 250
NIANAFPHIK CTVYDLPHVI ADSPGYSEVH CVAGDMFKFI PKADAIMMKC
260 270 280 290 300
ILHDWDDKEC IEILKRCKEA VPVKGGKVII VDIVLNVQSE HPYTKMRLTL
310 320 330 340
DLDMMLNTGG KERTEEEWKK LIHDAGYKGH KITQITAVQS VIEAYPY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D29811 mRNA Translation: BAB08004.1 |
Genome annotation databases
KEGGi | ag:BAB08004 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D29811 mRNA Translation: BAB08004.1 |
3D structure databases
SMRi | Q9LEL6 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:BAB08004 |
Enzyme and pathway databases
UniPathwayi | UPA00306;UER00441 |
BRENDAi | 2.1.1.128, 1610 |
SABIO-RKi | Q9LEL6 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR012967, Plant_MeTrfase_dimerisation IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11746, PTHR11746, 1 hit |
Pfami | View protein in Pfam PF08100, Dimerisation, 1 hit PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 6OMT_COPJA | |
Accessioni | Q9LEL6Primary (citable) accession number: Q9LEL6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 24, 2001 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 84 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families