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UniProtKB - Q9LE06 (BCAT4_ARATH)
Protein
Methionine aminotransferase BCAT4
Gene
BCAT4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Converts 2-oxo acids to branched-chain amino acids. Shows activity with L-Leu, L-Ile and L-Val as amino donors and alpha-keto-glutarate as an amino acceptor, but no activity for D-isomers of Leu, Ile, Val, Asp, Glu or Ala. Acts on methionine and its derivatives and the corresponding 2-oxo acids. Catalyzes the initial deamination of methionine to 4-methylthio-2-oxobutyrate as well as the transamination of other typical intermediates of the methionine chain elongation pathway.
2 PublicationsMiscellaneous
Although all the other members of the family possess a branched-chain amino acid aminotransferase activity, the exact function of BCAT4 remains unclear at present.
Catalytic activityi
- a 2-oxocarboxylate + L-methionine = 4-methylsulfanyl-2-oxobutanoate + an L-α-amino acid2 PublicationsEC:2.6.1.882 Publications
Cofactori
pyridoxal 5'-phosphateCurated
Kineticsi
- KM=0.045 mM for 2-oxo acid 4-methylthio-2-oxobutyrate (MTOB)1 Publication
- KM=0.93 mM for methionine1 Publication
- KM=4.86 mM for leucine1 Publication
- KM=1.61 mM for L-leucine1 Publication
- Vmax=2.7 µmol/min/mg enzyme with MTOB as substrate1 Publication
- Vmax=0.089 µmol/min/mg enzyme with methionine as substrate1 Publication
- Vmax=0.11 µmol/min/mg enzyme with leucine as substrate1 Publication
GO - Molecular functioni
- branched-chain-amino-acid transaminase activity Source: GO_Central
- methionine-oxo-acid transaminase activity Source: TAIR
GO - Biological processi
- branched-chain amino acid metabolic process Source: InterPro
- glucosinolate biosynthetic process Source: TAIR
- response to light stimulus Source: UniProtKB
- response to wounding Source: UniProtKB
Keywordsi
Molecular function | Aminotransferase, Transferase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | ARA:AT3G19710-MONOMER |
BRENDAi | 2.6.1.42, 399 2.6.1.88, 399 |
SABIO-RKi | Q9LE06 |
Names & Taxonomyi
Protein namesi | Recommended name: Methionine aminotransferase BCAT4 (EC:2.6.1.88)Alternative name(s): Branched-chain-amino-acid aminotransferase 4 Short name: Atbcat-4 Methionine-oxo-acid transaminase BCAT4 |
Gene namesi | Name:BCAT4 Ordered Locus Names:At3g19710 ORF Names:MMB12_16 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G19710 |
TAIRi | locus:2091216, AT3G19710 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Cytosol
- cytosol Source: TAIR
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Reduced methionine-derived aliphatic glucosinolate accumulation, but increased levels of free methionine and S-methylmethionine.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000103297 | 1 – 354 | Methionine aminotransferase BCAT4Add BLAST | 354 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 198 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
PaxDbi | Q9LE06 |
PRIDEi | Q9LE06 |
ProteomicsDBi | 241130 |
PTM databases
iPTMneti | Q9LE06 |
Expressioni
Tissue specificityi
Mostly expressed in phloem.1 Publication
Inductioni
Transient induction by wounding. Follows a diurnal rhythm.1 Publication
Gene expression databases
ExpressionAtlasi | Q9LE06, baseline and differential |
Genevisiblei | Q9LE06, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 6841, 1 interactor |
STRINGi | 3702.AT3G19710.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | KOG0975, Eukaryota |
HOGENOMi | CLU_031922_4_0_1 |
InParanoidi | Q9LE06 |
OMAi | ACISTTY |
OrthoDBi | 853728at2759 |
PhylomeDBi | Q9LE06 |
Family and domain databases
CDDi | cd01557, BCAT_beta_family, 1 hit |
Gene3Di | 3.20.10.10, 1 hit 3.30.470.10, 1 hit |
InterProi | View protein in InterPro IPR001544, Aminotrans_IV IPR036038, Aminotransferase-like IPR005786, B_amino_transII IPR043132, BCAT-like_C IPR043131, BCAT-like_N IPR033939, BCAT_family |
PANTHERi | PTHR42825, PTHR42825, 1 hit |
Pfami | View protein in Pfam PF01063, Aminotran_4, 1 hit |
PIRSFi | PIRSF006468, BCAT1, 1 hit |
SUPFAMi | SSF56752, SSF56752, 1 hit |
TIGRFAMsi | TIGR01123, ilvE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9LE06-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPSAQPLPV SVSDEKYANV KWEELAFKFV RTDYMYVAKC NHGESFQEGK
60 70 80 90 100
ILPFADLQLN PCAAVLQYGQ GLYEGLKAYR TEDGRILLFR PDQNGLRLQA
110 120 130 140 150
GADRLYMPYP SVDQFVSAIK QVALANKKWI PPPGKGTLYI RPILFGSGPI
160 170 180 190 200
LGSFPIPETT FTAFACPVGR YHKDNSGLNL KIEDQFRRAF PSGTGGVKSI
210 220 230 240 250
TNYCPVWIPL AEAKKQGFSD ILFLDAATGK NIEELFAANV FMLKGNVVST
260 270 280 290 300
PTIAGTILPG VTRNCVMELC RDFGYQVEER TIPLVDFLDA DEAFCTGTAS
310 320 330 340 350
IVTSIASVTF KDKKTGFKTG EETLAAKLYE TLSDIQTGRV EDTKGWTVEI
DRQG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ271732 mRNA Translation: CAB93129.1 AP000417 Genomic DNA Translation: BAB02558.1 CP002686 Genomic DNA Translation: AEE76278.1 AY052676 mRNA Translation: AAK96580.1 AF446892 mRNA Translation: AAL38625.1 AK226525 mRNA Translation: BAE98664.1 |
PIRi | T52401 |
RefSeqi | NP_188605.1, NM_112861.4 |
Genome annotation databases
EnsemblPlantsi | AT3G19710.1; AT3G19710.1; AT3G19710 |
GeneIDi | 821508 |
Gramenei | AT3G19710.1; AT3G19710.1; AT3G19710 |
KEGGi | ath:AT3G19710 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ271732 mRNA Translation: CAB93129.1 AP000417 Genomic DNA Translation: BAB02558.1 CP002686 Genomic DNA Translation: AEE76278.1 AY052676 mRNA Translation: AAK96580.1 AF446892 mRNA Translation: AAL38625.1 AK226525 mRNA Translation: BAE98664.1 |
PIRi | T52401 |
RefSeqi | NP_188605.1, NM_112861.4 |
3D structure databases
AlphaFoldDBi | Q9LE06 |
SMRi | Q9LE06 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 6841, 1 interactor |
STRINGi | 3702.AT3G19710.1 |
PTM databases
iPTMneti | Q9LE06 |
Proteomic databases
PaxDbi | Q9LE06 |
PRIDEi | Q9LE06 |
ProteomicsDBi | 241130 |
Genome annotation databases
EnsemblPlantsi | AT3G19710.1; AT3G19710.1; AT3G19710 |
GeneIDi | 821508 |
Gramenei | AT3G19710.1; AT3G19710.1; AT3G19710 |
KEGGi | ath:AT3G19710 |
Organism-specific databases
Araporti | AT3G19710 |
TAIRi | locus:2091216, AT3G19710 |
Phylogenomic databases
eggNOGi | KOG0975, Eukaryota |
HOGENOMi | CLU_031922_4_0_1 |
InParanoidi | Q9LE06 |
OMAi | ACISTTY |
OrthoDBi | 853728at2759 |
PhylomeDBi | Q9LE06 |
Enzyme and pathway databases
BioCyci | ARA:AT3G19710-MONOMER |
BRENDAi | 2.6.1.42, 399 2.6.1.88, 399 |
SABIO-RKi | Q9LE06 |
Miscellaneous databases
PROi | PR:Q9LE06 |
Gene expression databases
ExpressionAtlasi | Q9LE06, baseline and differential |
Genevisiblei | Q9LE06, AT |
Family and domain databases
CDDi | cd01557, BCAT_beta_family, 1 hit |
Gene3Di | 3.20.10.10, 1 hit 3.30.470.10, 1 hit |
InterProi | View protein in InterPro IPR001544, Aminotrans_IV IPR036038, Aminotransferase-like IPR005786, B_amino_transII IPR043132, BCAT-like_C IPR043131, BCAT-like_N IPR033939, BCAT_family |
PANTHERi | PTHR42825, PTHR42825, 1 hit |
Pfami | View protein in Pfam PF01063, Aminotran_4, 1 hit |
PIRSFi | PIRSF006468, BCAT1, 1 hit |
SUPFAMi | SSF56752, SSF56752, 1 hit |
TIGRFAMsi | TIGR01123, ilvE_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BCAT4_ARATH | |
Accessioni | Q9LE06Primary (citable) accession number: Q9LE06 Secondary accession number(s): Q0WW34 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2002 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families