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UniProtKB - Q9LE06 (BCAT4_ARATH)

Entry version 147 (25 May 2022)
Sequence version 1 (01 Oct 2000)
Previous versions | rss
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Protein

Methionine aminotransferase BCAT4

Gene

BCAT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Converts 2-oxo acids to branched-chain amino acids. Shows activity with L-Leu, L-Ile and L-Val as amino donors and alpha-keto-glutarate as an amino acceptor, but no activity for D-isomers of Leu, Ile, Val, Asp, Glu or Ala. Acts on methionine and its derivatives and the corresponding 2-oxo acids. Catalyzes the initial deamination of methionine to 4-methylthio-2-oxobutyrate as well as the transamination of other typical intermediates of the methionine chain elongation pathway.

2 Publications

Miscellaneous

Although all the other members of the family possess a branched-chain amino acid aminotransferase activity, the exact function of BCAT4 remains unclear at present.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

pyridoxal 5'-phosphateCurated

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.045 mM for 2-oxo acid 4-methylthio-2-oxobutyrate (MTOB)1 Publication
  2. KM=0.93 mM for methionine1 Publication
  3. KM=4.86 mM for leucine1 Publication
  4. KM=1.61 mM for L-leucine1 Publication
  1. Vmax=2.7 µmol/min/mg enzyme with MTOB as substrate1 Publication
  2. Vmax=0.089 µmol/min/mg enzyme with methionine as substrate1 Publication
  3. Vmax=0.11 µmol/min/mg enzyme with leucine as substrate1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

  • branched-chain amino acid metabolic process Source: InterPro
  • glucosinolate biosynthetic process Source: TAIR
  • response to light stimulus Source: UniProtKB
  • response to wounding Source: UniProtKB
Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		ARA:AT3G19710-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.6.1.42, 399
2.6.1.88, 399

SABIO-RK: Biochemical Reaction Kinetics Database

More...SABIO-RKi		Q9LE06

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Methionine aminotransferase BCAT4 (EC:2.6.1.88)
Alternative name(s):
Branched-chain-amino-acid aminotransferase 4
Short name:
Atbcat-4
Methionine-oxo-acid transaminase BCAT4
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:BCAT4
Ordered Locus Names:At3g19710
ORF Names:MMB12_16
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3

Organism-specific databases

Arabidopsis Information Portal

More...Araporti		AT3G19710

The Arabidopsis Information Resource

More...TAIRi		locus:2091216, AT3G19710

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Reduced methionine-derived aliphatic glucosinolate accumulation, but increased levels of free methionine and S-methylmethionine.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001032971 – 354Methionine aminotransferase BCAT4Add BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei198N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q9LE06

PRoteomics IDEntifications database

More...PRIDEi		Q9LE06

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		241130

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q9LE06

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Mostly expressed in phloem.1 Publication

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Transient induction by wounding. Follows a diurnal rhythm.1 Publication

Gene expression databases

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q9LE06, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q9LE06, AT

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		6841, 1 interactor

STRING: functional protein association networks

More...STRINGi		3702.AT3G19710.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		Q9LE06

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q9LE06

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG0975, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_031922_4_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q9LE06

Identification of Orthologs from Complete Genome Data

More...OMAi		ACISTTY

Database of Orthologous Groups

More...OrthoDBi		853728at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q9LE06

Family and domain databases

Conserved Domains Database

More...CDDi		cd01557, BCAT_beta_family, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.20.10.10, 1 hit
3.30.470.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR001544, Aminotrans_IV
IPR036038, Aminotransferase-like
IPR005786, B_amino_transII
IPR043132, BCAT-like_C
IPR043131, BCAT-like_N
IPR033939, BCAT_family

The PANTHER Classification System

More...PANTHERi		PTHR42825, PTHR42825, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01063, Aminotran_4, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF006468, BCAT1, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF56752, SSF56752, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR01123, ilvE_II, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9LE06-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPSAQPLPV SVSDEKYANV KWEELAFKFV RTDYMYVAKC NHGESFQEGK 
        60         70         80         90        100
ILPFADLQLN PCAAVLQYGQ GLYEGLKAYR TEDGRILLFR PDQNGLRLQA 
       110        120        130        140        150
GADRLYMPYP SVDQFVSAIK QVALANKKWI PPPGKGTLYI RPILFGSGPI 
       160        170        180        190        200
LGSFPIPETT FTAFACPVGR YHKDNSGLNL KIEDQFRRAF PSGTGGVKSI 
       210        220        230        240        250
TNYCPVWIPL AEAKKQGFSD ILFLDAATGK NIEELFAANV FMLKGNVVST 
       260        270        280        290        300
PTIAGTILPG VTRNCVMELC RDFGYQVEER TIPLVDFLDA DEAFCTGTAS 
       310        320        330        340        350
IVTSIASVTF KDKKTGFKTG EETLAAKLYE TLSDIQTGRV EDTKGWTVEI 

DRQG
Length:354
Mass (Da):39,019
Last modified:October 1, 2000 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i313EA4C5B5AE1898
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AJ271732 mRNA Translation: CAB93129.1
AP000417 Genomic DNA Translation: BAB02558.1
CP002686 Genomic DNA Translation: AEE76278.1
AY052676 mRNA Translation: AAK96580.1
AF446892 mRNA Translation: AAL38625.1
AK226525 mRNA Translation: BAE98664.1

Protein sequence database of the Protein Information Resource

More...PIRi		T52401

NCBI Reference Sequences

More...RefSeqi		NP_188605.1, NM_112861.4

Genome annotation databases

Ensembl plant genome annotation project

More...EnsemblPlantsi		AT3G19710.1; AT3G19710.1; AT3G19710

Database of genes from NCBI RefSeq genomes

More...GeneIDi		821508

Gramene; a comparative resource for plants

More...Gramenei		AT3G19710.1; AT3G19710.1; AT3G19710

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ath:AT3G19710

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271732 mRNA Translation: CAB93129.1
AP000417 Genomic DNA Translation: BAB02558.1
CP002686 Genomic DNA Translation: AEE76278.1
AY052676 mRNA Translation: AAK96580.1
AF446892 mRNA Translation: AAL38625.1
AK226525 mRNA Translation: BAE98664.1
PIRiT52401
RefSeqiNP_188605.1, NM_112861.4

3D structure databases

AlphaFoldDBiQ9LE06
SMRiQ9LE06
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi6841, 1 interactor
STRINGi3702.AT3G19710.1

PTM databases

iPTMnetiQ9LE06

Proteomic databases

PaxDbiQ9LE06
PRIDEiQ9LE06
ProteomicsDBi241130

Genome annotation databases

EnsemblPlantsiAT3G19710.1; AT3G19710.1; AT3G19710
GeneIDi821508
GrameneiAT3G19710.1; AT3G19710.1; AT3G19710
KEGGiath:AT3G19710

Organism-specific databases

AraportiAT3G19710
TAIRilocus:2091216, AT3G19710

Phylogenomic databases

eggNOGiKOG0975, Eukaryota
HOGENOMiCLU_031922_4_0_1
InParanoidiQ9LE06
OMAiACISTTY
OrthoDBi853728at2759
PhylomeDBiQ9LE06

Enzyme and pathway databases

BioCyciARA:AT3G19710-MONOMER
BRENDAi2.6.1.42, 399
2.6.1.88, 399
SABIO-RKiQ9LE06

Miscellaneous databases

Protein Ontology

More...PROi		PR:Q9LE06

Gene expression databases

ExpressionAtlasiQ9LE06, baseline and differential
GenevisibleiQ9LE06, AT

Family and domain databases

CDDicd01557, BCAT_beta_family, 1 hit
Gene3Di3.20.10.10, 1 hit
3.30.470.10, 1 hit
InterProiView protein in InterPro
IPR001544, Aminotrans_IV
IPR036038, Aminotransferase-like
IPR005786, B_amino_transII
IPR043132, BCAT-like_C
IPR043131, BCAT-like_N
IPR033939, BCAT_family
PANTHERiPTHR42825, PTHR42825, 1 hit
PfamiView protein in Pfam
PF01063, Aminotran_4, 1 hit
PIRSFiPIRSF006468, BCAT1, 1 hit
SUPFAMiSSF56752, SSF56752, 1 hit
TIGRFAMsiTIGR01123, ilvE_II, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBCAT4_ARATH
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9LE06
Secondary accession number(s): Q0WW34
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: October 1, 2000
Last modified: May 25, 2022
This is version 147 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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