UniProtKB - Q9LDI3 (CIPKO_ARATH)
CBL-interacting serine/threonine-protein kinase 24
CIPK24
Functioni
Involved in the regulatory pathway for the control of intracellular Na+ and K+ homeostasis and salt tolerance. Activates the vacuolar H+/Ca2+ antiporter CAX1 and operates in synergy with CBL4/SOS3 to activate the plasma membrane Na+/H+ antiporter SOS1. CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Phosphorylates CBL1, CBL4 and CBL10.
4 PublicationsCatalytic activityi
Cofactori
Kineticsi
- KM=131.2 µM for synthetic substrate1 Publication
- Vmax=1304 pmol/min/mg enzyme1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 40 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 134 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 17 – 25 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein kinase activity Source: TAIR
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
GO - Biological processi
- intracellular signal transduction Source: GO_Central
- protein phosphorylation Source: GO_Central
- response to salt stress Source: TAIR
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Manganese, Nucleotide-binding |
Enzyme and pathway databases
SABIO-RKi | Q9LDI3 |
Names & Taxonomyi
Protein namesi | Recommended name: CBL-interacting serine/threonine-protein kinase 24 (EC:2.7.11.1)Alternative name(s): Protein SALT OVERLY SENSITIVE 2 SNF1-related kinase 3.11 |
Gene namesi | Name:CIPK24 Synonyms:SnRK3.11, SOS2 Ordered Locus Names:At5g35410 ORF Names:F6I13.1, K21B8.3 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G35410 |
TAIRi | locus:2155233, AT5G35410 |
Subcellular locationi
Nucleus
- nucleus Source: UniProtKB-SubCell
Vacuole
- plant-type vacuole membrane Source: TAIR
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 40 | K → N: Abolishes autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 156 | S → D: Increases kinase activity. 1 Publication | 1 | |
Mutagenesisi | 168 | T → D: Increases kinase activity. 1 Publication | 1 | |
Mutagenesisi | 175 | Y → D: Increases kinase activity. 1 Publication | 1 | |
Mutagenesisi | 197 | G → E: Abolishes autophosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085877 | 1 – 446 | CBL-interacting serine/threonine-protein kinase 24Add BLAST | 446 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 156 | PhosphoserineBy similarity | 1 | |
Modified residuei | 168 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9LDI3 |
ProteomicsDBi | 246690 |
PTM databases
iPTMneti | Q9LDI3 |
Expressioni
Inductioni
Gene expression databases
ExpressionAtlasi | Q9LDI3, baseline and differential |
Genevisiblei | Q9LDI3, AT |
Interactioni
Subunit structurei
Interacts with CBL1, CBL2, CBL4/SOS3, CBL5, CBL9, CBL10 and with the protein phosphatase 2C ABI2.
6 PublicationsBinary interactionsi
Q9LDI3
Protein-protein interaction databases
BioGRIDi | 18756, 41 interactors |
DIPi | DIP-34745N |
IntActi | Q9LDI3, 32 interactors |
STRINGi | 3702.AT5G35410.1 |
Structurei
Secondary structure
3D structure databases
SMRi | Q9LDI3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9LDI3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 264 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 254 | |
Domaini | 305 – 329 | NAFPROSITE-ProRule annotationAdd BLAST | 25 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 152 – 179 | Activation loopAdd BLAST | 28 | |
Regioni | 336 – 365 | PPIAdd BLAST | 30 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
HOGENOMi | CLU_000288_59_0_1 |
InParanoidi | Q9LDI3 |
OrthoDBi | 1127668at2759 |
PhylomeDBi | Q9LDI3 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR018451, NAF/FISL_domain IPR004041, NAF_dom IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF03822, NAF, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50816, NAF, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MTKKMRRVGK YEVGRTIGEG TFAKVKFARN TDTGDNVAIK IMAKSTILKN
60 70 80 90 100
RMVDQIKREI SIMKIVRHPN IVRLYEVLAS PSKIYIVLEF VTGGELFDRI
110 120 130 140 150
VHKGRLEESE SRKYFQQLVD AVAHCHCKGV YHRDLKPENL LLDTNGNLKV
160 170 180 190 200
SDFGLSALPQ EGVELLRTTC GTPNYVAPEV LSGQGYDGSA ADIWSCGVIL
210 220 230 240 250
FVILAGYLPF SETDLPGLYR KINAAEFSCP PWFSAEVKFL IHRILDPNPK
260 270 280 290 300
TRIQIQGIKK DPWFRLNYVP IRAREEEEVN LDDIRAVFDG IEGSYVAENV
310 320 330 340 350
ERNDEGPLMM NAFEMITLSQ GLNLSALFDR RQDFVKRQTR FVSRREPSEI
360 370 380 390 400
IANIEAVANS MGFKSHTRNF KTRLEGLSSI KAGQLAVVIE IYEVAPSLFM
410 420 430 440
VDVRKAAGET LEYHKFYKKL CSKLENIIWR ATEGIPKSEI LRTITF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8BGJ7 | A0A1P8BGJ7_ARATH | Non-specific serine/threonine prote... | SOS2 ATSOS2, CIPK24, SALT OVERLY SENSITIVE 2, SNRK3.11, At5g35410 | 375 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF237670 Genomic DNA Translation: AAF62923.1 AF395081 mRNA Translation: AAK72257.1 AB025611 Genomic DNA Translation: BAA98146.1 AF262044 Genomic DNA Translation: AAF67384.1 Sequence problems. CP002688 Genomic DNA Translation: AED93966.1 AY099621 mRNA Translation: AAM20472.1 BT002138 mRNA Translation: AAN72149.1 |
RefSeqi | NP_198391.1, NM_122932.5 |
Genome annotation databases
EnsemblPlantsi | AT5G35410.1; AT5G35410.1; AT5G35410 |
GeneIDi | 833502 |
Gramenei | AT5G35410.1; AT5G35410.1; AT5G35410 |
KEGGi | ath:AT5G35410 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF237670 Genomic DNA Translation: AAF62923.1 AF395081 mRNA Translation: AAK72257.1 AB025611 Genomic DNA Translation: BAA98146.1 AF262044 Genomic DNA Translation: AAF67384.1 Sequence problems. CP002688 Genomic DNA Translation: AED93966.1 AY099621 mRNA Translation: AAM20472.1 BT002138 mRNA Translation: AAN72149.1 |
RefSeqi | NP_198391.1, NM_122932.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2EHB | X-ray | 2.10 | D | 304-446 | [»] | |
4D28 | X-ray | 3.30 | A/B/C/D | 1-446 | [»] | |
SMRi | Q9LDI3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 18756, 41 interactors |
DIPi | DIP-34745N |
IntActi | Q9LDI3, 32 interactors |
STRINGi | 3702.AT5G35410.1 |
PTM databases
iPTMneti | Q9LDI3 |
Proteomic databases
PaxDbi | Q9LDI3 |
ProteomicsDBi | 246690 |
Genome annotation databases
EnsemblPlantsi | AT5G35410.1; AT5G35410.1; AT5G35410 |
GeneIDi | 833502 |
Gramenei | AT5G35410.1; AT5G35410.1; AT5G35410 |
KEGGi | ath:AT5G35410 |
Organism-specific databases
Araporti | AT5G35410 |
TAIRi | locus:2155233, AT5G35410 |
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
HOGENOMi | CLU_000288_59_0_1 |
InParanoidi | Q9LDI3 |
OrthoDBi | 1127668at2759 |
PhylomeDBi | Q9LDI3 |
Enzyme and pathway databases
SABIO-RKi | Q9LDI3 |
Miscellaneous databases
EvolutionaryTracei | Q9LDI3 |
PROi | PR:Q9LDI3 |
Gene expression databases
ExpressionAtlasi | Q9LDI3, baseline and differential |
Genevisiblei | Q9LDI3, AT |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR018451, NAF/FISL_domain IPR004041, NAF_dom IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF03822, NAF, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50816, NAF, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CIPKO_ARATH | |
Accessioni | Q9LDI3Primary (citable) accession number: Q9LDI3 Secondary accession number(s): Q9LKR2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 21, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families