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UniProtKB - Q9LDH0 (XYLT_ARATH)
Protein
Beta-1,2-xylosyltransferase
Gene
XYLT
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Glycosyltransferase involved in the xylosylation of N-glycans (PubMed:10781814, PubMed:12943552, PubMed:15013764, PubMed:15686448).
Possesses beta-1,2-xylosyltransferase activity, transferring xylose from UDP-xylose to the core beta-linked mannose of N-glycans (PubMed:10781814, PubMed:12943552, PubMed:15013764, PubMed:15686448).
Involved in the biosynthesis of glycoprotein bound N-glycans (PubMed:15686448, PubMed:22024534).
Does not require metal ions for its activity (PubMed:15686448).
5 PublicationsMiscellaneous
Renders plant glycoproteins immunogenic and allergenic in human. This is due to the presence of beta-1,2-xylose and/or core alpha-1,3-fucose which are not found in mammalian proteins, and which constitute epitopes for carbohydrate-reactive antibodies.1 Publication
Catalytic activityi
- N4-{β-D-GlcNAc-(1→2)-α-D-Man-(1→3)-[β-D-GlcNAc-(1→2)-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-L-asparaginyl-[protein] + UDP-α-D-xylose = H+ + N4-{β-D-GlcNAc-(1→2)-α-D-Man-(1→3)-[β-D-GlcNAc-(1→2)-α-D-Man-(1→6)]-[β-D-Xyl-(1→2)]-β-D-Man-(1→4)-β-D-GlcNAcl-(1→4)-β-D-GlcNAc}-L-asparaginyl-[protein] + UDP2 PublicationsEC:2.4.2.382 Publications
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.CuratedView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- glycoprotein 2-beta-D-xylosyltransferase activity Source: UniProtKB
- glycosyltransferase activity Source: GO_Central
- UDP-xylosyltransferase activity Source: GO_Central
- xylosyltransferase activity Source: TAIR
GO - Biological processi
- posttranslational protein targeting to membrane, translocation Source: TAIR
- protein N-linked glycosylation Source: TAIR
- shoot system development Source: EnsemblPlants
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | ARA:AT5G55500-MONOMER |
BRENDAi | 2.4.2.38, 399 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT61, Glycosyltransferase Family 61 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-1,2-xylosyltransferase1 Publication (EC:2.4.2.382 Publications)Short name: AtXYLT1 Publication |
Gene namesi | Name:XYLT1 Publication Ordered Locus Names:At5g55500Imported ORF Names:MTE17.21Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G55500 |
TAIRi | locus:2173892, AT5G55500 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 2 Publications; Single-pass type II membrane protein 2 Publications
Note: Localizes in medial cisternae of Golgi apparatus.1 Publication
Golgi apparatus
- Golgi apparatus Source: TAIR
- Golgi medial cisterna Source: TAIR
- Golgi membrane Source: TAIR
- Golgi trans cisterna Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 34 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 35 – 534 | LumenalSequence analysisAdd BLAST | 500 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 53 | S → A: Abolishes stability and activity; when associated with A-303. 1 Publication | 1 | |
Mutagenesisi | 303 | T → A: Abolishes stability and activity; when associated with A-53. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080576 | 1 – 534 | Beta-1,2-xylosyltransferaseAdd BLAST | 534 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 51 | N-linked (GlcNAc...) asparagineSequence analysis2 Publications | 1 | |
Glycosylationi | 301 | N-linked (GlcNAc...) asparagineSequence analysis2 Publications | 1 | |
Glycosylationi | 479 | N-linked (GlcNAc...) asparagineSequence analysis1 Publication | 1 |
Post-translational modificationi
Glycosylation at least at one of the two sites Asn-51 and Asn-301 is necessary for enzyme stability and activity.1 Publication
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q9LDH0 |
ProteomicsDBi | 242556 |
PTM databases
iPTMneti | Q9LDH0 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9LDH0, baseline and differential |
Genevisiblei | Q9LDH0, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 20887, 2 interactors |
STRINGi | 3702.AT5G55500.1 |
Structurei
3D structure databases
AlphaFoldDBi | Q9LDH0 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4698, Eukaryota |
HOGENOMi | CLU_026674_0_0_1 |
InParanoidi | Q9LDH0 |
OMAi | WRSCEGY |
OrthoDBi | 567582at2759 |
PhylomeDBi | Q9LDH0 |
Family and domain databases
InterProi | View protein in InterPro IPR007657, Glycosyltransferase_61 |
PANTHERi | PTHR20961, PTHR20961, 1 hit |
Pfami | View protein in Pfam PF04577, Glyco_transf_61, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9LDH0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKRNPKILK IFLYMLLLNS LFLIIYFVFH SSSFSPEQSQ PPHIYHVSVN
60 70 80 90 100
NQSAIQKPWP ILPSYLPWTP PQRNLPTGSC EGYFGNGFTK RVDFLKPRIG
110 120 130 140 150
GGGEGSWFRC FYSETLQSSI CEGRNLRMVP DRIVMSRGGE KLEEVMGRKE
160 170 180 190 200
EEELPAFRQG AFEVAEEVSS RLGFKRHRRF GGGEGGSAVS RRLVNDEMLN
210 220 230 240 250
EYMQEGGIDR HTMRDLVASI RAVDTNDFVC EEWVEEPTLL VTRFEYANLF
260 270 280 290 300
HTVTDWYSAY VSSRVTGLPN RPHVVFVDGH CTTQLEETWT ALFSGIRYAK
310 320 330 340 350
NFTKPVCFRH AILSPLGYET ALFKGLSGEI DCKGDSAHNL WQNPDDKRTA
360 370 380 390 400
RISEFGEMIR AAFGLPVNRH RSLEKPLSSS SSSASVYNVL FVRREDYLAH
410 420 430 440 450
PRHGGKVQSR LINEEEVFDS LHHWVATGST GLTKCGINLV NGLLAHMSMK
460 470 480 490 500
DQVRAIQDAS VIIGAHGAGL THIVSATPNT TIFEIISVEF QRPHFELIAK
510 520 530
WKGLEYHAMH LANSRAEPTA VIEKLTEIMK SLGC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 54 | A → S in AAF77064 (Ref. 3) Curated | 1 | |
Sequence conflicti | 125 | N → S in AAF77064 (Ref. 3) Curated | 1 | |
Sequence conflicti | 383 | Missing in AAF77064 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ272121 mRNA Translation: CAB90610.1 AJ277603 mRNA Translation: CAB89489.1 AF272852 mRNA Translation: AAF77064.1 AB015479 Genomic DNA Translation: BAB08567.1 CP002688 Genomic DNA Translation: AED96635.1 AF411785 mRNA Translation: AAL06475.1 AY143907 mRNA Translation: AAN28846.1 |
PIRi | T52649 |
RefSeqi | NP_568825.1, NM_124932.4 |
Genome annotation databases
EnsemblPlantsi | AT5G55500.1; AT5G55500.1; AT5G55500 |
GeneIDi | 835643 |
Gramenei | AT5G55500.1; AT5G55500.1; AT5G55500 |
KEGGi | ath:AT5G55500 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ272121 mRNA Translation: CAB90610.1 AJ277603 mRNA Translation: CAB89489.1 AF272852 mRNA Translation: AAF77064.1 AB015479 Genomic DNA Translation: BAB08567.1 CP002688 Genomic DNA Translation: AED96635.1 AF411785 mRNA Translation: AAL06475.1 AY143907 mRNA Translation: AAN28846.1 |
PIRi | T52649 |
RefSeqi | NP_568825.1, NM_124932.4 |
3D structure databases
AlphaFoldDBi | Q9LDH0 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 20887, 2 interactors |
STRINGi | 3702.AT5G55500.1 |
Protein family/group databases
CAZyi | GT61, Glycosyltransferase Family 61 |
PTM databases
iPTMneti | Q9LDH0 |
Proteomic databases
PaxDbi | Q9LDH0 |
ProteomicsDBi | 242556 |
Genome annotation databases
EnsemblPlantsi | AT5G55500.1; AT5G55500.1; AT5G55500 |
GeneIDi | 835643 |
Gramenei | AT5G55500.1; AT5G55500.1; AT5G55500 |
KEGGi | ath:AT5G55500 |
Organism-specific databases
Araporti | AT5G55500 |
TAIRi | locus:2173892, AT5G55500 |
Phylogenomic databases
eggNOGi | KOG4698, Eukaryota |
HOGENOMi | CLU_026674_0_0_1 |
InParanoidi | Q9LDH0 |
OMAi | WRSCEGY |
OrthoDBi | 567582at2759 |
PhylomeDBi | Q9LDH0 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BioCyci | ARA:AT5G55500-MONOMER |
BRENDAi | 2.4.2.38, 399 |
Miscellaneous databases
PROi | PR:Q9LDH0 |
Gene expression databases
ExpressionAtlasi | Q9LDH0, baseline and differential |
Genevisiblei | Q9LDH0, AT |
Family and domain databases
InterProi | View protein in InterPro IPR007657, Glycosyltransferase_61 |
PANTHERi | PTHR20961, PTHR20961, 1 hit |
Pfami | View protein in Pfam PF04577, Glyco_transf_61, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | XYLT_ARATH | |
Accessioni | Q9LDH0Primary (citable) accession number: Q9LDH0 Secondary accession number(s): Q9LKK7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 16, 2001 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways