UniProtKB - Q9LDF2 (LOH1_ARATH)
Ceramide synthase LOH1
LOH1
Functioni
Essential for plant growth, promotes cell division in root meristems (PubMed:21666002, PubMed:21883234, PubMed:26276842).
Catalyzes the biosynthesis of ceramide sphingolipids with C(16) to C(28) fatty acids, structural membrane lipids involved in membrane trafficking (e.g. early endosomes) and cell polarity (e.g. polar auxin transport related proteins); mostly active with t18:0 and saturated very long saturated fatty acids (C24:0 and C26:0), such as long-chain base (LCB) phytosphingosine (t18:0), lignoceroyl- and hexacosanoyl-CoAs (PubMed:21883234, PubMed:26635357, PubMed:21666002, PubMed:26276842).
Mediates resistance to sphinganine-analog mycotoxins (SAMs, e.g. fumonisin B1) by restoring the sphingolipid biosynthesis (By similarity).
Could salvage the transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi apparatus in ceramides-depleted cells after SAM exposure (By similarity).
May prevent precocious cell death by delaying PR1 accumulation during aging (PubMed:21883234).
Contributes to hypoxic conditions tolerance (e.g. submergences), especially in the dark, by promoting the formation of very-long-chain (VLC) ceramide species (22:1, 24:1 and 26:1) and of VLC unsaturated ceramides, which are modulating CTR1-mediated ethylene signaling leading to endoplasmic reticulum (ER)-to-nucleus translocation of EIN2 and EIN3 (PubMed:25822663).
By similarity5 PublicationsCatalytic activityi
- (4R)-hydroxysphinganine + a fatty acyl-CoA = an N-acyl-(4R)-4-hydroxysphinganine + CoA + H+3 PublicationsThis reaction proceeds in the forward3 Publications direction.
- (4R)-hydroxysphinganine + hexacosanoyl-CoA = CoA + H+ + N-hexacosanoyl-(4R)-hydroxysphinganine3 PublicationsThis reaction proceeds in the forward3 Publications direction.
- (4R)-hydroxysphinganine + tetracosanoyl-CoA = CoA + H+ + N-tetracosanoyl-(4R)-hydroxysphinganine3 PublicationsThis reaction proceeds in the forward3 Publications direction.
Activity regulationi
Kineticsi
- KM=6.9 µM for phytosphingosine (t18:0)1 Publication
- Vmax=273 pmol/min/mg enzyme with phytosphingosine (t18:0) as substrate1 Publication
: Sphingolipid metabolism Pathwayi
This protein is involved in Sphingolipid metabolism.3 PublicationsView all proteins of this organism that are known to be involved in Sphingolipid metabolism.
GO - Molecular functioni
- N-acyltransferase activity Source: GO_Central
- sphingosine N-acyltransferase activity Source: TAIR
GO - Biological processi
- ceramide biosynthetic process Source: UniProtKB
- endomembrane system organization Source: UniProtKB
- regulation of auxin polar transport Source: UniProtKB
- sphingolipid metabolic process Source: UniProtKB
- very long-chain fatty acid biosynthetic process Source: TAIR
Keywordsi
Molecular function | Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.297, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Ceramide synthase LOH11 Publication (EC:2.3.1.-3 Publications)Short name: CS11 Publication Short name: CSII1 Publication Alternative name(s): Protein LONGEVITY ASSURANCE GENE ONE HOMOLOG 11 Publication Short name: LAG One Homolog 11 Publication Short name: LAG1 homolog 11 Publication Short name: LAG1 longevity assurance homolog 11 Publication |
Gene namesi | Name:LOH12 Publications Synonyms:LAG11 Publication Ordered Locus Names:At3g25540Imported ORF Names:MWL2.19Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G25540 |
TAIRi | locus:2094528, AT3G25540 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 16 – 36 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 85 – 105 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 131 – 151 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 157 – 177 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 216 – 236 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 260 – 280 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185518 | 1 – 310 | Ceramide synthase LOH1Add BLAST | 310 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 300 | PhosphoserineBy similarity | 1 | |
Modified residuei | 302 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9LDF2 |
ProteomicsDBi | 237069 |
PTM databases
iPTMneti | Q9LDF2 |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | Q9LDF2, baseline and differential |
Genevisiblei | Q9LDF2, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 76 – 289 | TLCPROSITE-ProRule annotationAdd BLAST | 214 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1607, Eukaryota |
HOGENOMi | CLU_028277_5_0_1 |
InParanoidi | Q9LDF2 |
OMAi | WEDFPKM |
OrthoDBi | 987268at2759 |
PhylomeDBi | Q9LDF2 |
Family and domain databases
InterProi | View protein in InterPro IPR016439, Lag1/Lac1-like IPR006634, TLC-dom |
PANTHERi | PTHR12560, PTHR12560, 1 hit |
Pfami | View protein in Pfam PF03798, TRAM_LAG1_CLN8, 1 hit |
PIRSFi | PIRSF005225, LAG1_LAC1, 1 hit |
SMARTi | View protein in SMART SM00724, TLC, 1 hit |
PROSITEi | View protein in PROSITE PS50922, TLC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGLFESVKSI DWEQESFPTY QDLGFLPLFA VFFPTIRFLL DRFVFEKLAS
60 70 80 90 100
LVIYGRMSTN KSDNIKDRKK NSPKVRKFKE SAWKCIYYLS AELLALSVTY
110 120 130 140 150
NEPWFSNTLY FWIGPGDQIW PDQPMKMKLK FLYMFAAGFY TYSIFALVFW
160 170 180 190 200
ETRRSDFGVS MGHHITTLVL IVLSYICRLT RAGSVILALH DASDVFLEIG
210 220 230 240 250
KMSKYCGAES LASISFVLFA LSWVVLRLIY YPFWILWSTS YQIIMTVDKE
260 270 280 290 300
KHPNGPILYY MFNTLLYFLL VLHIFWWVLI YRMLVKQVQD RGKLSEDVRS
310
DSESDDEHED
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1I9LTU4 | A0A1I9LTU4_ARATH | TRAM, LAG1 and CLN8 (TLC) lipid-sen... | LAG1 LAG One Homologue 1, LAG1 HOMOLOG 1, LOH1, LONGEVITY ASSURANCE GENE 1, At3g25540 | 242 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF198179 mRNA Translation: AAF66102.1 AB025639 Genomic DNA Translation: BAB01323.1 CP002686 Genomic DNA Translation: AEE77023.1 CP002686 Genomic DNA Translation: ANM66003.1 AF360152 mRNA Translation: AAK25862.1 AY142597 mRNA Translation: AAN13166.1 |
RefSeqi | NP_001319643.1, NM_001338755.1 NP_566769.1, NM_113450.5 |
Genome annotation databases
EnsemblPlantsi | AT3G25540.1; AT3G25540.1; AT3G25540 AT3G25540.3; AT3G25540.3; AT3G25540 |
GeneIDi | 822140 |
Gramenei | AT3G25540.1; AT3G25540.1; AT3G25540 AT3G25540.3; AT3G25540.3; AT3G25540 |
KEGGi | ath:AT3G25540 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF198179 mRNA Translation: AAF66102.1 AB025639 Genomic DNA Translation: BAB01323.1 CP002686 Genomic DNA Translation: AEE77023.1 CP002686 Genomic DNA Translation: ANM66003.1 AF360152 mRNA Translation: AAK25862.1 AY142597 mRNA Translation: AAN13166.1 |
RefSeqi | NP_001319643.1, NM_001338755.1 NP_566769.1, NM_113450.5 |
3D structure databases
SMRi | Q9LDF2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT3G25540.1 |
PTM databases
iPTMneti | Q9LDF2 |
Proteomic databases
PaxDbi | Q9LDF2 |
ProteomicsDBi | 237069 |
Genome annotation databases
EnsemblPlantsi | AT3G25540.1; AT3G25540.1; AT3G25540 AT3G25540.3; AT3G25540.3; AT3G25540 |
GeneIDi | 822140 |
Gramenei | AT3G25540.1; AT3G25540.1; AT3G25540 AT3G25540.3; AT3G25540.3; AT3G25540 |
KEGGi | ath:AT3G25540 |
Organism-specific databases
Araporti | AT3G25540 |
TAIRi | locus:2094528, AT3G25540 |
Phylogenomic databases
eggNOGi | KOG1607, Eukaryota |
HOGENOMi | CLU_028277_5_0_1 |
InParanoidi | Q9LDF2 |
OMAi | WEDFPKM |
OrthoDBi | 987268at2759 |
PhylomeDBi | Q9LDF2 |
Enzyme and pathway databases
BRENDAi | 2.3.1.297, 399 |
Miscellaneous databases
PROi | PR:Q9LDF2 |
Gene expression databases
ExpressionAtlasi | Q9LDF2, baseline and differential |
Genevisiblei | Q9LDF2, AT |
Family and domain databases
InterProi | View protein in InterPro IPR016439, Lag1/Lac1-like IPR006634, TLC-dom |
PANTHERi | PTHR12560, PTHR12560, 1 hit |
Pfami | View protein in Pfam PF03798, TRAM_LAG1_CLN8, 1 hit |
PIRSFi | PIRSF005225, LAG1_LAC1, 1 hit |
SMARTi | View protein in SMART SM00724, TLC, 1 hit |
PROSITEi | View protein in PROSITE PS50922, TLC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LOH1_ARATH | |
Accessioni | Q9LDF2Primary (citable) accession number: Q9LDF2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways