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Entry version 50 (11 Dec 2019)
Sequence version 1 (01 Oct 2000)
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Protein

Aclacinomycin-T 2-deoxy-L-fucose transferase

Gene

aknK

Organism
Streptomyces galilaeus
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the biosynthesis of the trisaccharide moiety characteristic of the antitumor drug aclacinomycins. In the first reaction, AknK catalyzes the transfer of 2-deoxy-beta-L-fucose from the activated donor dTDP-2-deoxy-beta-L-fucose to the mono-glycosylated aclacinomycin T (rhodosaminyl aklavinone), forming the di-glycosylated aclacinomycin S (L-2-deoxyfucosyl-L-rhodosaminyl aklavinone). It can also catalyze the addition of an alternate dTDP-L-sugar, dTDP-L-daunosamine, to aclacinomycin T and the addition of 2-deoxy-beta-L-fucose to the mono-glycosylated aglycones (monoglycosylated anthracyclines) such as daunomycin (daunorubicin), adriamycin (doxorubicin) and idarubicin. In vitro, AknK also catalyzes the addition of a second L-2-deoxyfucosyl moiety from dTDP-2-deoxy-beta-L-fucose, albeit with reduced activity, to the natural disaccharide chain of aclacinomycin S to produce L-deoxyfucosyl-L-deoxyfucosyl-L-rhodosaminyl aklavinone (2-deoxy-alpha-D-fucosyl-aclacinomycin S), a variant of the natural aclacinomycin A.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Kcat is 2.1 sec(-1) for transferase activity with rhodosaminyl aklavinone as sugar acceptor and TDP-L-daunosamine as sugar donor. Kcat is 5.4 sec(-1) for transferase activity with idarubicin as sugar acceptor and TDP-L-2-deoxyfucose as sugar donor. Kcat is 65.4 sec(-1) for transferase activity with rhodosaminyl aklavinone as sugar acceptor and TDP-L-2-deoxyfucose as sugar donor.1 Publication
  1. KM=104.4 µM for rhodosaminyl aklavinone (with TDP-L-daunosamine as sugar donor)1 Publication
  2. KM=109.1 µM for rhodosaminyl aklavinone (with TDP-L-2-deoxyfucose as sugar donor)1 Publication
  3. KM=138 µM for idarubicin (with TDP-L-2-deoxyfucose as sugar donor)1 Publication
  4. KM=148.9 µM for TDP-L-2-deoxyfucose (with rhodosaminyl aklavinone as sugar acceptor)1 Publication
  5. KM=531 µM for TDP-L-2-deoxyfucose (with idarubicin as sugar acceptor)1 Publication
  6. KM=940 µM for TDP-L-daunosamine (with rhodosaminyl aklavinone as sugar acceptor)1 Publication

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    Biological processAntibiotic biosynthesis

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MetaCyc:MONOMER-18193

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    2.4.1.B46 13206

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    Q9L555

    Protein family/group databases

    Carbohydrate-Active enZymes

    More...
    CAZyi
    GT1 Glycosyltransferase Family 1

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Aclacinomycin-T 2-deoxy-L-fucose transferase1 Publication (EC:2.4.1.3271 Publication)
    Short name:
    AknK1 Publication
    Alternative name(s):
    L-2-deoxyfucosyltransferase1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:aknK1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiStreptomyces galilaeus
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri33899 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004306751 – 440Aclacinomycin-T 2-deoxy-L-fucose transferaseAdd BLAST440

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q9L555

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the glycosyltransferase 28 family.Curated

    Phylogenomic databases

    KEGG Orthology (KO)

    More...
    KOi
    K15948

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR010610 DUF1205
    IPR030953 Glycosyl_450act

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF06722 DUF1205, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR04516 glycosyl_450act, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    Q9L555-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKVLFTTFAA KSHMHAQVPL AWALQTAGHE VRIASQPDLA EDITRTGLTA
    60 70 80 90 100
    VCVGEPLLLE EQMQRVNEGL GDDAEIMESQ AEAGMDMTET RPEMLTWDHV
    110 120 130 140 150
    LGVFTSMTAM AFQNSCPERM IDDVVAFARE WQPDLIVWDT LSFAGPVAAQ
    160 170 180 190 200
    VTGAAHARLL FGLDLLGRMR ETFLDLQEER LPEQRDDPLR EWLTWTLGRY
    210 220 230 240 250
    GAEFEEEVAV GQWTVDPVPP SMRFPVKQPF VPLRYIPYNG QAVIPDWLHE
    260 270 280 290 300
    PPKKRRVCLT LGVAHREVLD GDRASIGELV EALAELDVEV VATLNEKQLA
    310 320 330 340 350
    GMELPDNVRA VDFVPLNALL PTCSAVIHHG GSGTFQTALA HGVPQLIVPD
    360 370 380 390 400
    MVWDTIHKAK QLERFGAGLY LHDVDNYTAQ DLRDHLLRLL EEPSFAENCA
    410 420 430 440
    RIRREMVGTP SPNDIVPLLE KLTAEHRRDR GARGTVRGEQ
    Length:440
    Mass (Da):49,164
    Last modified:October 1, 2000 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA167FE0E0FC42CF0
    GO

    <p>This subsection of the ‘Sequence’ section reports information derived from mass spectrometry experiments done on the entire protein or on biologically active derived peptide(s).<p><a href='/help/mass_spectrometry' target='_top'>More...</a></p>Mass spectrometryi

    Molecular mass is 50699 Da. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AF257324 Genomic DNA Translation: AAF70102.1

    Genome annotation databases

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ag:AAF70102

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF257324 Genomic DNA Translation: AAF70102.1

    3D structure databases

    SMRiQ9L555
    ModBaseiSearch...

    Protein family/group databases

    CAZyiGT1 Glycosyltransferase Family 1

    Genome annotation databases

    KEGGiag:AAF70102

    Phylogenomic databases

    KOiK15948

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18193
    BRENDAi2.4.1.B46 13206
    SABIO-RKiQ9L555

    Family and domain databases

    InterProiView protein in InterPro
    IPR010610 DUF1205
    IPR030953 Glycosyl_450act
    PfamiView protein in Pfam
    PF06722 DUF1205, 1 hit
    TIGRFAMsiTIGR04516 glycosyl_450act, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAKNK_STRGJ
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9L555
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 29, 2014
    Last sequence update: October 1, 2000
    Last modified: December 11, 2019
    This is version 50 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
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