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UniProtKB - Q9KWU4 (PYC_BACSU)
Protein
Pyruvate carboxylase
Gene
pyc
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second, leading to oxaloacetate production. Fulfills an anaplerotic function in B.subtilis as it is necessary for growth on glucose, but is not required for sporulation.
1 PublicationCatalytic activityi
- EC:6.4.1.1
Cofactori
Activity regulationi
Allosterically activated by acetyl-CoA. Inhibited by the biotin-complexing protein avidin.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 121 | ATPBy similarity | 1 | |
Binding sitei | 205 | ATPBy similarity | 1 | |
Sitei | 234 | Involved in CO(2) fixationSequence analysis | 1 | |
Binding sitei | 240 | ATPBy similarity | 1 | |
Active sitei | 242 | Sequence analysis | 1 | |
Metal bindingi | 543 | Divalent metal cationBy similarity | 1 | |
Binding sitei | 615 | SubstrateBy similarity | 1 | |
Metal bindingi | 712 | Divalent metal cation; via carbamate groupBy similarity | 1 | |
Metal bindingi | 741 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 743 | Divalent metal cationBy similarity | 1 | |
Binding sitei | 876 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- biotin binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- pyruvate carboxylase activity Source: GO_Central
GO - Biological processi
- gluconeogenesis Source: GO_Central
- pyruvate metabolic process Source: GO_Central
Keywordsi
Molecular function | Ligase, Multifunctional enzyme |
Ligand | ATP-binding, Biotin, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU14860-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Pyruvate carboxylase (EC:6.4.1.1)Alternative name(s): Pyruvic carboxylase Short name: PYC |
Gene namesi | Name:pyc Synonyms:pycA, ylaP Ordered Locus Names:BSU14860 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000146826 | 1 – 1148 | Pyruvate carboxylaseAdd BLAST | 1148 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 712 | N6-carboxylysineBy similarity | 1 | |
Modified residuei | 1112 | N6-biotinyllysinePROSITE-ProRule annotationBy similarity | 1 |
Proteomic databases
jPOSTi | Q9KWU4 |
PaxDbi | Q9KWU4 |
PRIDEi | Q9KWU4 |
Expressioni
Inductioni
Constitutively expressed.Curated
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 224308.BSU14860 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 457 | Biotin carboxylationAdd BLAST | 457 | |
Domaini | 125 – 321 | ATP-graspPROSITE-ProRule annotationAdd BLAST | 197 | |
Domaini | 534 – 802 | Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST | 269 | |
Domaini | 1071 – 1146 | Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 542 – 546 | Substrate bindingBy similarity | 5 |
Phylogenomic databases
eggNOGi | COG1038, Bacteria |
InParanoidi | Q9KWU4 |
OMAi | YAIQSRV |
PhylomeDBi | Q9KWU4 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR011761, ATP-grasp IPR005481, BC-like_N IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR003379, Carboxylase_cons_dom IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR016185, PreATP-grasp_dom_sf IPR000891, PYR_CT IPR005930, Pyruv_COase IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif |
Pfami | View protein in Pfam PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit PF00682, HMGL-like, 1 hit PF02436, PYC_OADA, 1 hit |
PIRSFi | PIRSF001594, Pyruv_carbox, 1 hit |
SMARTi | View protein in SMART SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01235, pyruv_carbox, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit PS50991, PYR_CT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9KWU4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQQSIQKVL VANRGEIAIR IFRACTELNI RTVAVYSKED SGSYHRYKAD
60 70 80 90 100
EAYLVGEGKK PIDAYLDIEG IIDIAKRNKV DAIHPGYGFL SENIHFARRC
110 120 130 140 150
EEEGIVFIGP KSEHLDMFGD KVKAREQAEK AGIPVIPGSD GPAETLEAVE
160 170 180 190 200
QFGQANGYPI IIKASLGGGG RGMRIVRSES EVKEAYERAK SEAKAAFGND
210 220 230 240 250
EVYVEKLIEN PKHIEVQVIG DKQGNVVHLF ERDCSVQRRH QKVIEVAPSV
260 270 280 290 300
SLSPELRDQI CEAAVALAKN VNYINAGTVE FLVANNEFYF IEVNPRVQVE
310 320 330 340 350
HTITEMITGV DIVQTQILVA QGHSLHSKKV NIPEQKDIFT IGYAIQSRVT
360 370 380 390 400
TEDPQNDFMP DTGKIMAYRS GGGFGVRLDT GNSFQGAVIT PYYDSLLVKL
410 420 430 440 450
STWALTFEQA AAKMVRNLQE FRIRGIKTNI PFLENVAKHE KFLTGQYDTS
460 470 480 490 500
FIDTTPELFN FPKQKDRGTK MLTYIGNVTV NGFPGIGKKE KPAFDKPLGV
510 520 530 540 550
KVDVDQQPAR GTKQILDEKG AEGLANWVKE QKSVLLTDTT FRDAHQSLLA
560 570 580 590 600
TRIRSHDLKK IANPTAALWP ELFSMEMWGG ATFDVAYRFL KEDPWKRLED
610 620 630 640 650
LRKEVPNTLF QMLLRSSNAV GYTNYPDNVI KEFVKQSAQS GIDVFRIFDS
660 670 680 690 700
LNWVKGMTLA IDAVRDTGKV AEAAICYTGD ILDKNRTKYD LAYYTSMAKE
710 720 730 740 750
LEAAGAHILG IKDMAGLLKP QAAYELVSAL KETIDIPVHL HTHDTSGNGI
760 770 780 790 800
YMYAKAVEAG VDIIDVAVSS MAGLTSQPSA SGFYHAMEGN DRRPEMNVQG
810 820 830 840 850
VELLSQYWES VRKYYSEFES GMKSPHTEIY EHEMPGGQYS NLQQQAKGVG
860 870 880 890 900
LGDRWNEVKE MYRRVNDMFG DIVKVTPSSK VVGDMALYMV QNNLTEKDVY
910 920 930 940 950
EKGESLDFPD SVVELFKGNI GQPHGGFPEK LQKLILKGQE PITVRPGELL
960 970 980 990 1000
EPVSFEAIKQ EFKEQHNLEI SDQDAVAYAL YPKVFTDYVK TTESYGDISV
1010 1020 1030 1040 1050
LDTPTFFYGM TLGEEIEVEI ERGKTLIVKL ISIGEPQPDA TRVVYFELNG
1060 1070 1080 1090 1100
QPREVVIKDE SIKSSVQERL KADRTNPSHI AASMPGTVIK VLAEAGTKVN
1110 1120 1130 1140
KGDHLMINEA MKMETTVQAP FSGTIKQVHV KNGEPIQTGD LLLEIEKA
Sequence cautioni
The sequence CAB09721 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13359.1 Z97025 Genomic DNA Translation: CAB09721.1 Frameshift. Z98682 Genomic DNA Translation: CAB11339.1 |
PIRi | F69685 |
RefSeqi | NP_389369.1, NC_000964.3 WP_003244778.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13359; CAB13359; BSU_14860 |
GeneIDi | 935920 |
KEGGi | bsu:BSU14860 |
PATRICi | fig|224308.179.peg.1620 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13359.1 Z97025 Genomic DNA Translation: CAB09721.1 Frameshift. Z98682 Genomic DNA Translation: CAB11339.1 |
PIRi | F69685 |
RefSeqi | NP_389369.1, NC_000964.3 WP_003244778.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | Q9KWU4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU14860 |
Proteomic databases
jPOSTi | Q9KWU4 |
PaxDbi | Q9KWU4 |
PRIDEi | Q9KWU4 |
Genome annotation databases
EnsemblBacteriai | CAB13359; CAB13359; BSU_14860 |
GeneIDi | 935920 |
KEGGi | bsu:BSU14860 |
PATRICi | fig|224308.179.peg.1620 |
Phylogenomic databases
eggNOGi | COG1038, Bacteria |
InParanoidi | Q9KWU4 |
OMAi | YAIQSRV |
PhylomeDBi | Q9KWU4 |
Enzyme and pathway databases
BioCyci | BSUB:BSU14860-MONOMER |
Miscellaneous databases
PROi | PR:Q9KWU4 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR011761, ATP-grasp IPR005481, BC-like_N IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR003379, Carboxylase_cons_dom IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR016185, PreATP-grasp_dom_sf IPR000891, PYR_CT IPR005930, Pyruv_COase IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif |
Pfami | View protein in Pfam PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit PF00682, HMGL-like, 1 hit PF02436, PYC_OADA, 1 hit |
PIRSFi | PIRSF001594, Pyruv_carbox, 1 hit |
SMARTi | View protein in SMART SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01235, pyruv_carbox, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit PS50991, PYR_CT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PYC_BACSU | |
Accessioni | Q9KWU4Primary (citable) accession number: Q9KWU4 Secondary accession number(s): O07640, Q9KWU5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |