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Protein

Microbial collagenase

Gene

vcc

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Possesses gelatinolytic activity.

Catalytic activityi

Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1'; Pro and Ala at P2 and P2'; and hydroxyproline, Ala or Arg at P3'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi435Zinc; catalyticPROSITE-ProRule annotation1
Active sitei436PROSITE-ProRule annotation1
Metal bindingi439Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • collagen catabolic process Source: UniProtKB-KW
  • proteolysis Source: TIGR

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processCollagen degradation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciVCHO:VC1650-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Microbial collagenase (EC:3.4.24.3)
Alternative name(s):
Exoprotease Vcc
Gene namesi
Name:vcc
Ordered Locus Names:VC_1650
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000002867830 – 818Microbial collagenaseAdd BLAST789

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiQ9KRJ0

Interactioni

Protein-protein interaction databases

STRINGi243277.VC1650

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M9A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105KXC Bacteria
ENOG410XSXR LUCA
KOiK01387
OMAiRYAAVYV

Family and domain databases

InterProiView protein in InterPro
IPR007280 Peptidase_C_arc/bac
IPR013661 Peptidase_M9_N_dom
IPR002169 Peptidase_M9A/M9B
PfamiView protein in Pfam
PF01752 Peptidase_M9, 1 hit
PF08453 Peptidase_M9_N, 1 hit
PF04151 PPC, 1 hit
PRINTSiPR00931 MICOLLPTASE
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9KRJ0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSFQQRIQHH PIAWACVIAG LSYSSYSQAA CEIQDLQPAR DLPAQIAVAT
60 70 80 90 100
QACYNSWFYA PTATLDNLYS EASLAHLQTV LDAEIARYTG EAQQARRLEN
110 120 130 140 150
YGEFIRAAYY VRYNAGREPY SQALSQRFAQ SIDRFLRHPH AFDQGREQVG
160 170 180 190 200
AMKSLSLMVD NVKQLPLTMD AMILALHRFN RETAQDTQWV DGLNNLFRAM
210 220 230 240 250
SGHVGNSEFY RYLAANTQHI DTLYRFALDN EWALETDAEF LVYNALRETG
260 270 280 290 300
RLLISPDAIT KQKARHVMRQ VIARYPLGSK HDKLWLAAVE MLHYYAPEVL
310 320 330 340 350
QQLGIDLDAA KRDLAARILP NRFECQGPAI IRSQDLSDAQ AAQACDVLDK
360 370 380 390 400
KEQDFHQVAN TGLAPVADDY NTRVEVVVFA NNSSYVNYSS FLFGNTTDNG
410 420 430 440 450
GQYLEGNPAD QNNQARFVAY RYANDADLSI LNLEHEYTHY LDARFNQYGS
460 470 480 490 500
FSDNLAHGHI VWWLEGFAEY MHYKQGYQAA VKLISQGKLS LSDVFATTYS
510 520 530 540 550
NDTNRIYRWG YLAVRFMLEK HPQDVESLLA LSRTGQFDQW AQSVKLLGER
560 570 580 590 600
YNTEFSAWLD TLQRDNPDNP DNPDNPDNPD NPDNPEQPNP EPNAVTQLAA
610 620 630 640 650
NSSLTLTGKA YSEHLFYVDV PEYSREFHVQ ISGEGDADLY MSYQQVAHYY
660 670 680 690 700
DYQVTEFTYG SNEQITFKPE QNGYIKPGRY YLSVTGRADY SAVILNTHLV
710 720 730 740 750
TEQPNEQPTI KDDLAPVLLE AGNSQSLTVH RQRYVAIYVP KGVSEVQVWL
760 770 780 790 800
TASEQNRGNV DLFAAKAYWP TREQFEHAST GAGSHEYLRI PVTQEGYVHF
810
SLNAQQLGDT VEMVAYFD
Length:818
Mass (Da):92,997
Last modified:October 1, 2000 - v1
Checksum:iD71F97018C4FE30D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti574 – 585Missing (Ref. 1) CuratedAdd BLAST12
Sequence conflicti704P → L in AAD20592 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109145 Genomic DNA Translation: AAD20592.1
AE003852 Genomic DNA Translation: AAF94801.1
PIRiF82173
RefSeqiNP_231287.1, NC_002505.1
WP_000009926.1, NC_002505.1

Genome annotation databases

EnsemblBacteriaiAAF94801; AAF94801; VC_1650
GeneIDi2613781
KEGGivch:VC1650
PATRICifig|243277.26.peg.1577

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109145 Genomic DNA Translation: AAD20592.1
AE003852 Genomic DNA Translation: AAF94801.1
PIRiF82173
RefSeqiNP_231287.1, NC_002505.1
WP_000009926.1, NC_002505.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC1650

Proteomic databases

PRIDEiQ9KRJ0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF94801; AAF94801; VC_1650
GeneIDi2613781
KEGGivch:VC1650
PATRICifig|243277.26.peg.1577

Phylogenomic databases

eggNOGiENOG4105KXC Bacteria
ENOG410XSXR LUCA
KOiK01387
OMAiRYAAVYV

Enzyme and pathway databases

BioCyciVCHO:VC1650-MONOMER

Family and domain databases

InterProiView protein in InterPro
IPR007280 Peptidase_C_arc/bac
IPR013661 Peptidase_M9_N_dom
IPR002169 Peptidase_M9A/M9B
PfamiView protein in Pfam
PF01752 Peptidase_M9, 1 hit
PF08453 Peptidase_M9_N, 1 hit
PF04151 PPC, 1 hit
PRINTSiPR00931 MICOLLPTASE
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCOLA_VIBCH
AccessioniPrimary (citable) accession number: Q9KRJ0
Secondary accession number(s): Q9X4F8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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