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Protein

5'-nucleotidase

Gene

nutA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Degradation of extracellular 5'-nucleotides for nutritional needs.By similarity

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • chlorideBy similarityNote: Chloride.By similarity
  • Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Divalent metal cation 1By similarity1
Metal bindingi47Divalent metal cation 1By similarity1
Metal bindingi88Divalent metal cation 1By similarity1
Metal bindingi88Divalent metal cation 2By similarity1
Metal bindingi120Divalent metal cation 2By similarity1
Sitei121Transition state stabilizerBy similarity1
Sitei124Transition state stabilizerBy similarity1
Metal bindingi221Divalent metal cation 2By similarity1
Metal bindingi256Divalent metal cation 2By similarity1
Metal bindingi258Divalent metal cation 1By similarity1
Binding sitei432SubstrateBy similarity1

GO - Molecular functioni

  • 5'-nucleotidase activity Source: TIGR
  • metal ion binding Source: UniProtKB-KW
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • nucleotide catabolic process Source: InterPro
  • nucleotide-sugar metabolic process Source: TIGR

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVCHO:VC2174-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
5'-nucleotidase (EC:3.1.3.5)
Gene namesi
Name:nutA
Ordered Locus Names:VC_2174
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cell outer membrane By similarity; Lipid-anchor PROSITE-ProRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21PROSITE-ProRule annotationAdd BLAST21
ChainiPRO_000000002822 – 5535'-nucleotidaseAdd BLAST532

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi22N-palmitoyl cysteineCurated1
Lipidationi22S-diacylglycerol cysteineCurated1

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Protein-protein interaction databases

STRINGi243277.VC2174

Structurei

3D structure databases

ProteinModelPortaliQ9KQ30
SMRiQ9KQ30
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni501 – 507Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CGH Bacteria
COG0737 LUCA
KOiK11751
OMAiCRFQECN

Family and domain databases

Gene3Di3.60.21.10, 1 hit
3.90.780.10, 1 hit
InterProiView protein in InterPro
IPR008334 5'-Nucleotdase_C
IPR036907 5'-Nucleotdase_C_sf
IPR006146 5'-Nucleotdase_CS
IPR006179 5_nucleotidase/apyrase
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
PANTHERiPTHR11575 PTHR11575, 1 hit
PfamiView protein in Pfam
PF02872 5_nucleotid_C, 1 hit
PF00149 Metallophos, 1 hit
PRINTSiPR01607 APYRASEFAMLY
SUPFAMiSSF55816 SSF55816, 1 hit
PROSITEiView protein in PROSITE
PS00785 5_NUCLEOTIDASE_1, 1 hit
PS00786 5_NUCLEOTIDASE_2, 1 hit
PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9KQ30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQGLILKSV LSAAIIASLA GCATAPAQQW EADKTYKLTI LHTNDHHGRF
60 70 80 90 100
WQNQYGEYGM AARKTLIDQL RADIEAQGGS VLLLSGGDIN TGVPESDLQD
110 120 130 140 150
AEPDFKGMSK IGYDAMALGN HEFDNPLEVL FKQKEWANFP MLSANIYDKA
160 170 180 190 200
TGKRLFEPYH IFDKQGIKIA VIGLTTEDTA KIGNPEYIGG IDFRDPKEEA
210 220 230 240 250
KKVIAELKKK EKPDLIIAVT HMGHYQNGEH GVNAPGDVAL ARYLPAGELD
260 270 280 290 300
MIVGGHSQEP VCMEGPNLVK KNFKPGDECK PDIQNGTYIV QAYEWGKYVG
310 320 330 340 350
RADYEFRNGE LNMVSYNLIP VNLKKKVEVN GETQRVFATS EIKEDSAMLE
360 370 380 390 400
FLRPFQEKGQ EQLSIKIAHS NGKLEGDRNV VRFEQTNLGR MIAMAHMQRA
410 420 430 440 450
KADFAVMNSG GVRDSIQAGD ITYKDVLKVQ PFGNIVSYVD MNGQEVLDYL
460 470 480 490 500
NVVATKPVDS GAYAQFAGIS MTVADGKVSN VVIGGKQLRL DATYRFTVPS
510 520 530 540 550
FNAAGGDGYP KITDHPGYVN TGFVDAEVLK DYLEANSPID VNRFAPAGEI

VYR
Length:553
Mass (Da):60,903
Last modified:October 1, 2000 - v1
Checksum:i5AA621C27526B311
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA Translation: AAF95319.1
PIRiE82108
RefSeqiNP_231805.1, NC_002505.1
WP_000809002.1, NC_002505.1

Genome annotation databases

EnsemblBacteriaiAAF95319; AAF95319; VC_2174
GeneIDi2613310
KEGGivch:VC2174
PATRICifig|243277.26.peg.2072

Similar proteinsi

Entry informationi

Entry namei5NTD_VIBCH
AccessioniPrimary (citable) accession number: Q9KQ30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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