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Entry version 113 (12 Aug 2020)
Sequence version 1 (01 Oct 2000)
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Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mn2+By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GDP.
Proteins known to be involved in this subpathway in this organism are:
  1. Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT)
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GDP, the pathway ppGpp biosynthesis and in Purine metabolism.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase
LigandManganese

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
VCHO:VC2710-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00908;UER00886

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:spoT
Ordered Locus Names:VC_2710
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri243277 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000584 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Essential for growth, it cannot be disrupted. It can be disrupted in a relA deletion strain (relA phosphorylates GTP to ppGpp).1 Publication

Miscellaneous databases

Pathogen-Host Interaction database

More...
PHI-basei
PHI:4167

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003864181 – 705Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolaseAdd BLAST705

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with CgtA/Obg (AC Q9KUS8) in a yeast 2-hybrid assay.

1 Publication

Protein-protein interaction databases

Database of interacting proteins

More...
DIPi
DIP-60892N

Protein interaction database and analysis system

More...
IntActi
Q9KNM2, 1 interactor

STRING: functional protein association networks

More...
STRINGi
243277.VC_2710

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini45 – 144HDPROSITE-ProRule annotationAdd BLAST100
Domaini387 – 448TGSPROSITE-ProRule annotationAdd BLAST62
Domaini631 – 705ACTPROSITE-ProRule annotationAdd BLAST75

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0317, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_012300_3_0_6

KEGG Orthology (KO)

More...
KOi
K01139

Identification of Orthologs from Complete Genome Data

More...
OMAi
HYYSIYQ

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00077, HDc, 1 hit
cd05399, NT_Rel-Spo_like, 1 hit
cd01668, TGS_RSH, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.10.20.30, 1 hit
3.30.460.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002912, ACT_dom
IPR012675, Beta-grasp_dom_sf
IPR003607, HD/PDEase_dom
IPR043519, NT_sf
IPR004811, RelA/Spo_fam
IPR007685, RelA_SpoT
IPR004095, TGS
IPR012676, TGS-like
IPR033655, TGS_RelA

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF13291, ACT_4, 1 hit
PF04607, RelA_SpoT, 1 hit
PF02824, TGS, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00471, HDc, 1 hit
SM00954, RelA_SpoT, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF81271, SSF81271, 1 hit
SSF81301, SSF81301, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00691, spoT_relA, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51671, ACT, 1 hit
PS51831, HD, 1 hit
PS51880, TGS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9KNM2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYLFDSLKDV AQEYLTEPQI EALRQSYVVA RDAHEGQTRS SGEPYIIHPV
60 70 80 90 100
AVARILAEMR LDLETLQAAL LHDVIEDCDV TKEDLDAHFG SSVAELVDGV
110 120 130 140 150
SKLDKLKFRD RKEAQAENFR KMVLAMVQDI RVILIKLADR TPNMRTLGAL
160 170 180 190 200
RPDKKRRIAR ETLEIYAPLA HRLGIHNIKT ELEELGFEAL YPNRYRVLKE
210 220 230 240 250
VVKAARGNRK EMIQRIHSEI EGRLQEVGLP ARVVGREKNL FSIYNKMKTK
260 270 280 290 300
EQRFHTIMDI YAFRIVVDTA DTCYRVLGQV HSLYKPRPAR MKDYIAVPKA
310 320 330 340 350
NGYQSLHTSM VGPHGVPVEV QIRTEDMDQM ADKGVAAHWS YKANSERGGT
360 370 380 390 400
TAQIKAQRWM QSLLELQQSA GNSFEFIENV KSDLFPDEIY VFTPKGRIVE
410 420 430 440 450
LPMGATAVDF AYAVHTDIGN TCVGARVDRT PYPLSQSLKS GQTVEIISAP
460 470 480 490 500
GARPNAAWLN YVVTSRARTK IRQVLKTMRR EDSITLGRRL LNHALGEHSV
510 520 530 540 550
NEIAPENISK VLSDLKIASM DDLLAAIGLG ELMSIVIARR LLGNADELTE
560 570 580 590 600
PSKSGGNKNK LPIRGAEGIL LTFANCCHPI PDDHIIAHVS PGRGLVVHRE
610 620 630 640 650
TCPNVRGYQK EPDKYMAVEW TKDYDQEFIT ELKVDMHNRQ GALAELTNVI
660 670 680 690 700
SKTGSNIHGL STEERDGRLY TVTVLLTTKD RVHLAGIMRK IRTMPHALKV

RRRKN
Length:705
Mass (Da):79,496
Last modified:October 1, 2000 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i08F756E3A576B07F
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE003852 Genomic DNA Translation: AAF95850.1

Protein sequence database of the Protein Information Resource

More...
PIRi
B82044

NCBI Reference Sequences

More...
RefSeqi
NP_232337.1, NC_002505.1
WP_010895463.1, NC_002505.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAF95850; AAF95850; VC_2710

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
vch:VC2710

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|243277.26.peg.2585

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003852 Genomic DNA Translation: AAF95850.1
PIRiB82044
RefSeqiNP_232337.1, NC_002505.1
WP_010895463.1, NC_002505.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

DIPiDIP-60892N
IntActiQ9KNM2, 1 interactor
STRINGi243277.VC_2710

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
2615538

Genome annotation databases

EnsemblBacteriaiAAF95850; AAF95850; VC_2710
KEGGivch:VC2710
PATRICifig|243277.26.peg.2585

Phylogenomic databases

eggNOGiCOG0317, Bacteria
HOGENOMiCLU_012300_3_0_6
KOiK01139
OMAiHYYSIYQ

Enzyme and pathway databases

UniPathwayiUPA00908;UER00886
BioCyciVCHO:VC2710-MONOMER

Miscellaneous databases

PHI-baseiPHI:4167

Family and domain databases

CDDicd00077, HDc, 1 hit
cd05399, NT_Rel-Spo_like, 1 hit
cd01668, TGS_RSH, 1 hit
Gene3Di3.10.20.30, 1 hit
3.30.460.10, 1 hit
InterProiView protein in InterPro
IPR002912, ACT_dom
IPR012675, Beta-grasp_dom_sf
IPR003607, HD/PDEase_dom
IPR043519, NT_sf
IPR004811, RelA/Spo_fam
IPR007685, RelA_SpoT
IPR004095, TGS
IPR012676, TGS-like
IPR033655, TGS_RelA
PfamiView protein in Pfam
PF13291, ACT_4, 1 hit
PF04607, RelA_SpoT, 1 hit
PF02824, TGS, 1 hit
SMARTiView protein in SMART
SM00471, HDc, 1 hit
SM00954, RelA_SpoT, 1 hit
SUPFAMiSSF81271, SSF81271, 1 hit
SSF81301, SSF81301, 1 hit
TIGRFAMsiTIGR00691, spoT_relA, 1 hit
PROSITEiView protein in PROSITE
PS51671, ACT, 1 hit
PS51831, HD, 1 hit
PS51880, TGS, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPOT_VIBCH
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9KNM2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: October 1, 2000
Last modified: August 12, 2020
This is version 113 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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