UniProtKB - Q9KN42 (VGRG3_VIBCH)
Type VI secretion system spike protein VgrG3
vgrG3
Functioni
Part of the type VI secretion system specialized secretion system, which delivers several virulence factors in both prokaryotic and eukaryotic cells during infection (PubMed:23362380, PubMed:23341465).
Forms the spike at the tip of the elongating tube formed by haemolysin co-regulated protein Hcp. Allows the delivery of the TseL antibacterial toxin to target cells where it exerts its toxicity (PubMed:23362380).
Additionally, acts directly as an effector and targets the cell wall peptidoglycan layer of prey cells for degradation via its C-terminus (PubMed:23362380, PubMed:23341465).
Toxicity is counteracted by a cognate immunity protein TsiV3 (PubMed:23362380, PubMed:24699653, PubMed:23341465).
3 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 842 | 1 Publication | 1 |
GO - Molecular functioni
- hydrolase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Names & Taxonomyi
Protein namesi | Recommended name: Type VI secretion system spike protein VgrG31 PublicationAlternative name(s): Muramidase Vgrg3C1 Publication (EC:3.2.1.-3 Publications) |
Gene namesi | Name:vgrG31 Publication Ordered Locus Names:VC_A0123 |
Organismi | Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) |
Taxonomic identifieri | 243277 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 827 | E → Q: Almost complete loss of enzymatic activity. 1 Publication | 1 | |
Mutagenesisi | 842 | D → N: Complete loss of enzymatic activity. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000448967 | 1 – 1017 | Type VI secretion system spike protein VgrG3Add BLAST | 1017 |
Interactioni
Subunit structurei
Binary interactionsi
Q9KN42
With | #Exp. | IntAct |
---|---|---|
tsiV3 [Q9KN41] | 11 | EBI-9356343,EBI-9356338 |
Protein-protein interaction databases
IntActi | Q9KN42, 1 interactor |
MINTi | Q9KN42 |
STRINGi | 243277.VC_A0123 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9KN42 |
SMRi | Q9KN42 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG3409, Bacteria COG3501, Bacteria |
HOGENOMi | CLU_004121_0_1_6 |
OMAi | VWTHRYE |
Family and domain databases
Gene3Di | 1.10.101.10, 1 hit 2.40.50.230, 1 hit |
InterProi | View protein in InterPro IPR006531, Gp5/Vgr_OB IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf IPR036366, PGBDSf IPR017847, T6SS_RhsGE_Vgr_subset IPR006533, T6SS_Vgr_RhsGE IPR037026, Vgr_OB-fold_dom_sf |
Pfami | View protein in Pfam PF01471, PG_binding_1, 1 hit PF04717, Phage_base_V, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit |
TIGRFAMsi | TIGR01646, vgr_GE, 1 hit TIGR03361, VI_Rhs_Vgr, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MARLQFQLKV DGLEDESLVV RGFEGQESLS DSVWRCEPCY GFRYQVDLAS
60 70 80 90 100
ALSNLTAEQF VDQTAHLTIL RDGQVVQQIN GIVRQLSKGD TGHRHTFYSL
110 120 130 140 150
TLVPALERLS LRSNSRIFQQ QSVPEIISIL LQEMGIEDYA FALKRECAQR
160 170 180 190 200
EFCVQYRETD LQFLHRIAAE EGLVYSHLHE AQKHTLLFTD SSDSQPKLAK
210 220 230 240 250
PVPYNALAGG EINLPYVVDL QFKTTAQVSH TELKDYSFKK PAYGFTQRTQ
260 270 280 290 300
GKDIAYQQPN YEHFDAPGRY KDDANGKAFS QIRLEYLRRD ALLADAKSDE
310 320 330 340 350
PLLLAGVRFD LQDHLDHAMN RDWLVVQANH QGTQPQALQE EGGSGATTYS
360 370 380 390 400
NQLKLIPAHI TWRARPCAKP QVDGPMIATV VGPQGEEIYC DNFGRVKVHF
410 420 430 440 450
PWDRYSSSNE KSSCWVRVAQ EWAGSQYGSM AIPRVGHEVI VSFLNGDPDQ
460 470 480 490 500
PIITGRTYHA TNTAPYALPD HKTKTVLRTE THQGQGYNEL SFEDQAGSEQ
510 520 530 540 550
ILLHAQKDWD ALIEHDHTEV IRHDQHLTVD NDRFTRIQRN QHLTVEGEVR
560 570 580 590 600
SKIALDSSHE VGASLQHKVG QRIAVEAGKE ISLKSGAKIV VEAGAELTLK
610 620 630 640 650
AGGSFVKVDA GGVHLVGPAI NLNAGGSAGS GSAYGGQLAA APRMLAQAKP
660 670 680 690 700
VAELVQPDIA ASMQSGAARV IDVASLPTMM PSSANNTAND EPVAEEKTPE
710 720 730 740 750
RILKSDLLKP SDELEKLAKR QASAYRQGNH SDEVKLLQEA LIKLGFDLGK
760 770 780 790 800
AGADGDFGSK TKTAIEQFQK SYQPSHQTHP SYSIGAVDGI VGKGTLLALD
810 820 830 840 850
EALMDGWVYE NNIYQIWPLG KTSEKYESAG RGPGVISTGN GDYGGASYGC
860 870 880 890 900
YQMSSNLGVV QKYIQSSKFK EFFSGLNPAT KEFNVVWQDI ASRYPQEFRE
910 920 930 940 950
EQHQFIKRTH YDIQIGHLRG KGLLFEHNRA AVHDLIWSTS VQFGGRTNLI
960 970 980 990 1000
FNALNGQNME SMTDKDIIIL VQDYKLVNTE RLFKSSPSWW SDLKKRAVSE
1010
KKALLELEID GLEVDIK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE003853 Genomic DNA Translation: AAF96037.1 |
PIRi | F82500 |
RefSeqi | NP_232524.1, NC_002506.1 WP_000113295.1, NZ_LT906615.1 |
Genome annotation databases
EnsemblBacteriai | AAF96037; AAF96037; VC_A0123 |
GeneIDi | 57741584 |
KEGGi | vch:VC_A0123 |
PATRICi | fig|243277.26.peg.2763 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE003853 Genomic DNA Translation: AAF96037.1 |
PIRi | F82500 |
RefSeqi | NP_232524.1, NC_002506.1 WP_000113295.1, NZ_LT906615.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4NOO | X-ray | 2.30 | A/C | 813-1017 | [»] | |
4NSO | X-ray | 2.40 | A | 731-1009 | [»] | |
AlphaFoldDBi | Q9KN42 | |||||
SMRi | Q9KN42 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q9KN42, 1 interactor |
MINTi | Q9KN42 |
STRINGi | 243277.VC_A0123 |
Protocols and materials databases
DNASUi | 2612828 |
Genome annotation databases
EnsemblBacteriai | AAF96037; AAF96037; VC_A0123 |
GeneIDi | 57741584 |
KEGGi | vch:VC_A0123 |
PATRICi | fig|243277.26.peg.2763 |
Phylogenomic databases
eggNOGi | COG3409, Bacteria COG3501, Bacteria |
HOGENOMi | CLU_004121_0_1_6 |
OMAi | VWTHRYE |
Family and domain databases
Gene3Di | 1.10.101.10, 1 hit 2.40.50.230, 1 hit |
InterProi | View protein in InterPro IPR006531, Gp5/Vgr_OB IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf IPR036366, PGBDSf IPR017847, T6SS_RhsGE_Vgr_subset IPR006533, T6SS_Vgr_RhsGE IPR037026, Vgr_OB-fold_dom_sf |
Pfami | View protein in Pfam PF01471, PG_binding_1, 1 hit PF04717, Phage_base_V, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit |
TIGRFAMsi | TIGR01646, vgr_GE, 1 hit TIGR03361, VI_Rhs_Vgr, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VGRG3_VIBCH | |
Accessioni | Q9KN42Primary (citable) accession number: Q9KN42 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2020 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families