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Entry version 99 (12 Aug 2020)
Sequence version 1 (01 Oct 2000)
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Protein

Type VI secretion system spike protein VgrG3

Gene

vgrG3

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Part of the type VI secretion system specialized secretion system, which delivers several virulence factors in both prokaryotic and eukaryotic cells during infection (PubMed:23362380, PubMed:23341465). Forms the spike at the tip of the elongating tube formed by haemolysin co-regulated protein Hcp. Allows the delivery of the TseL antibacterial toxin to target cells where it exerts its toxicity (PubMed:23362380). Additionally, acts directly as an effector and targets the cell wall peptidoglycan layer of prey cells for degradation via its C-terminus (PubMed:23362380, PubMed:23341465). Toxicity is counteracted by a cognate immunity protein TsiV3 (PubMed:23362380, PubMed:24699653, PubMed:23341465).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei8421 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
VCHO:VCA0123-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Type VI secretion system spike protein VgrG31 Publication
Alternative name(s):
Muramidase Vgrg3C1 Publication (EC:3.2.1.-3 Publications)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:vgrG31 Publication
Ordered Locus Names:VC_A0123
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri243277 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000584 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Mutants are unable to secrete TseL.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi827E → Q: Almost complete loss of enzymatic activity. 1 Publication1
Mutagenesisi842D → N: Complete loss of enzymatic activity. 2 Publications1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004489671 – 1017Type VI secretion system spike protein VgrG3Add BLAST1017

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with TsiV3 (PubMed:24751834, PubMed:24699653, PubMed:23341465).

Interacts with TseL (PubMed:23362380).

4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
Q9KN42, 1 interactor

Molecular INTeraction database

More...
MINTi
Q9KN42

STRING: functional protein association networks

More...
STRINGi
243277.VC_A0123

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11017
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q9KN42

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the VgrG protein family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG3409, Bacteria
COG3501, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_004121_0_1_6

KEGG Orthology (KO)

More...
KOi
K11904

Identification of Orthologs from Complete Genome Data

More...
OMAi
IMHAERN

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.101.10, 1 hit
2.40.50.230, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002477, Peptidoglycan-bd-like
IPR036365, PGBD-like_sf
IPR036366, PGBDSf
IPR017847, T6SS_RhsGE_Vgr_subset
IPR006533, T6SS_Vgr_RhsGE
IPR037026, Vgr_OB-fold_dom_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01471, PG_binding_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47090, SSF47090, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01646, vgr_GE, 1 hit
TIGR03361, VI_Rhs_Vgr, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9KN42-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MARLQFQLKV DGLEDESLVV RGFEGQESLS DSVWRCEPCY GFRYQVDLAS
60 70 80 90 100
ALSNLTAEQF VDQTAHLTIL RDGQVVQQIN GIVRQLSKGD TGHRHTFYSL
110 120 130 140 150
TLVPALERLS LRSNSRIFQQ QSVPEIISIL LQEMGIEDYA FALKRECAQR
160 170 180 190 200
EFCVQYRETD LQFLHRIAAE EGLVYSHLHE AQKHTLLFTD SSDSQPKLAK
210 220 230 240 250
PVPYNALAGG EINLPYVVDL QFKTTAQVSH TELKDYSFKK PAYGFTQRTQ
260 270 280 290 300
GKDIAYQQPN YEHFDAPGRY KDDANGKAFS QIRLEYLRRD ALLADAKSDE
310 320 330 340 350
PLLLAGVRFD LQDHLDHAMN RDWLVVQANH QGTQPQALQE EGGSGATTYS
360 370 380 390 400
NQLKLIPAHI TWRARPCAKP QVDGPMIATV VGPQGEEIYC DNFGRVKVHF
410 420 430 440 450
PWDRYSSSNE KSSCWVRVAQ EWAGSQYGSM AIPRVGHEVI VSFLNGDPDQ
460 470 480 490 500
PIITGRTYHA TNTAPYALPD HKTKTVLRTE THQGQGYNEL SFEDQAGSEQ
510 520 530 540 550
ILLHAQKDWD ALIEHDHTEV IRHDQHLTVD NDRFTRIQRN QHLTVEGEVR
560 570 580 590 600
SKIALDSSHE VGASLQHKVG QRIAVEAGKE ISLKSGAKIV VEAGAELTLK
610 620 630 640 650
AGGSFVKVDA GGVHLVGPAI NLNAGGSAGS GSAYGGQLAA APRMLAQAKP
660 670 680 690 700
VAELVQPDIA ASMQSGAARV IDVASLPTMM PSSANNTAND EPVAEEKTPE
710 720 730 740 750
RILKSDLLKP SDELEKLAKR QASAYRQGNH SDEVKLLQEA LIKLGFDLGK
760 770 780 790 800
AGADGDFGSK TKTAIEQFQK SYQPSHQTHP SYSIGAVDGI VGKGTLLALD
810 820 830 840 850
EALMDGWVYE NNIYQIWPLG KTSEKYESAG RGPGVISTGN GDYGGASYGC
860 870 880 890 900
YQMSSNLGVV QKYIQSSKFK EFFSGLNPAT KEFNVVWQDI ASRYPQEFRE
910 920 930 940 950
EQHQFIKRTH YDIQIGHLRG KGLLFEHNRA AVHDLIWSTS VQFGGRTNLI
960 970 980 990 1000
FNALNGQNME SMTDKDIIIL VQDYKLVNTE RLFKSSPSWW SDLKKRAVSE
1010
KKALLELEID GLEVDIK
Length:1,017
Mass (Da):112,995
Last modified:October 1, 2000 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iECFF8C66E0214FE4
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE003853 Genomic DNA Translation: AAF96037.1

Protein sequence database of the Protein Information Resource

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PIRi
F82500

NCBI Reference Sequences

More...
RefSeqi
NP_232524.1, NC_002506.1
WP_000113295.1, NZ_LT906615.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAF96037; AAF96037; VC_A0123

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
vch:VCA0123

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|243277.26.peg.2763

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA Translation: AAF96037.1
PIRiF82500
RefSeqiNP_232524.1, NC_002506.1
WP_000113295.1, NZ_LT906615.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NOOX-ray2.30A/C813-1017[»]
4NSOX-ray2.40A731-1009[»]
SMRiQ9KN42
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

IntActiQ9KN42, 1 interactor
MINTiQ9KN42
STRINGi243277.VC_A0123

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
2612828

Genome annotation databases

EnsemblBacteriaiAAF96037; AAF96037; VC_A0123
KEGGivch:VCA0123
PATRICifig|243277.26.peg.2763

Phylogenomic databases

eggNOGiCOG3409, Bacteria
COG3501, Bacteria
HOGENOMiCLU_004121_0_1_6
KOiK11904
OMAiIMHAERN

Enzyme and pathway databases

BioCyciVCHO:VCA0123-MONOMER

Family and domain databases

Gene3Di1.10.101.10, 1 hit
2.40.50.230, 1 hit
InterProiView protein in InterPro
IPR002477, Peptidoglycan-bd-like
IPR036365, PGBD-like_sf
IPR036366, PGBDSf
IPR017847, T6SS_RhsGE_Vgr_subset
IPR006533, T6SS_Vgr_RhsGE
IPR037026, Vgr_OB-fold_dom_sf
PfamiView protein in Pfam
PF01471, PG_binding_1, 1 hit
SUPFAMiSSF47090, SSF47090, 1 hit
TIGRFAMsiTIGR01646, vgr_GE, 1 hit
TIGR03361, VI_Rhs_Vgr, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiVGRG3_VIBCH
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9KN42
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2020
Last sequence update: October 1, 2000
Last modified: August 12, 2020
This is version 99 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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