Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3'3'-cGAMP-specific phosphodiesterase 1

Gene

VC_A0681

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Phosphodiesterase (PDE) that catalyzes the hydrolysis of 3'3'-cyclic GMP-AMP (3'3'-cGAMP), leading to linear 5'-pApG. Also displays 5'-nucleotidase activity, further hydrolyzing 5'-pApG to 5'-ApG. Counteracts the function of the 3'3'-cGAMP synthase DncV, and is involved in the modulation of intracellular 3'3'-cGAMP levels. Enhances bacterial chemotaxis and inhibits intestinal colonization in vivo. Thus exerts a crucial role in regulating bacterial infectivity through catalyzing 3'3'-cGAMP degradation. Is specific for 3'3'-cGAMP since it cannot degrade other cGAMP linkage isomers (3'2'-, 2'3'-, and 2'2'-cGAMPs) (PubMed:25837739). Is also able to hydrolyze c-di-GMP but not c-di-AMP (PubMed:25837739, PubMed:25343965).2 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • cyclic-nucleotide phosphodiesterase activity Source: UniProtKB

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
VCHO:VCA0681-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
3'3'-cGAMP-specific phosphodiesterase 11 Publication (EC:3.1.4.-1 Publication)
Short name:
3'3'-cGAMP PDE 11 Publication
Short name:
V-cGAP11 Publication
Alternative name(s):
5'-pApG 5'-nucleotidase1 Publication (EC:3.1.3.-1 Publication)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Ordered Locus Names:VC_A0681Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri243277 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000584 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Significant increase in the ability to colonize the small intestine compared to the wild-type strain. No defect in biofilm formation. Enforced DncV expression in mutant cells lacking this gene causes an enhanced inhibition of chemotaxis. The double mutants lacking both VC_A0681 and VC_A0210 or both VC_A0681 and VC_A0931 show enhanced bacterial infectivity, and the triple one (VC_A0681, VC_A0210 and VC_A0931) has the highest infectivity, which demonstrates that V-cGAPs play non-redundant roles in cGAMP degradation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi74 – 75HD → AA: Reduced enzymatic activity. 1 Publication2
Mutagenesisi288 – 289HD → AA: Loss of enzymatic activity. 1 Publication2
Mutagenesisi291 – 292GK → AA: Reduced enzymatic activity. 1 Publication2
Mutagenesisi341 – 343HHE → AAA: Reduced enzymatic activity. 1 Publication3
Mutagenesisi346 – 347DG → AA: Reduced enzymatic activity. 1 Publication2
Mutagenesisi350Y → A: Loss of enzymatic activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004353521 – 4313'3'-cGAMP-specific phosphodiesterase 1Add BLAST431

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Expression is up-regulated by 3'3'-cGAMP production (at both mRNA and protein levels) (PubMed:25837739). Transcripts are more abundant in biofilm cells than in planktonic cells (PubMed:25343965).2 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
243277.VCA0681

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
Q9KLR1

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q9KLR1

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini39 – 155HDPROSITE-ProRule annotationAdd BLAST117
Domaini231 – 427HD-GYPPROSITE-ProRule annotationAdd BLAST197

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105DHT Bacteria
COG2206 LUCA

Identification of Orthologs from Complete Genome Data

More...
OMAi
HDCGVSS

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00077 HDc, 2 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR037522 HD_GYP_dom
IPR006675 HDIG_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01966 HD, 2 hits

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00471 HDc, 2 hits

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00277 HDIG, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51831 HD, 1 hit
PS51832 HD_GYP, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9KLR1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRWSEIGCTM KSVNIEWNVN LRQAFFCIAR ALDSVGVDDI NHGHRVGYMA
60 70 80 90 100
YSCAQAMEWS EEECQLVFAL GLIHDCGVAQ KRDFYRLLEN MQPDNTQQHC
110 120 130 140 150
VRGNELLSNC PPLAPFADAI LYHHTPWDEL KNIAISDRNK RFAALIFLAD
160 170 180 190 200
RVDYLKELYP RDEYGNVTQE ARNQVCLEIG RLSGSLFERD LVRTMQHLLS
210 220 230 240 250
KEFIWFSMEH HHIEAMGHNL PSTPFFEQKL GVEEIMSIAM LMANVVDAKS
260 270 280 290 300
QFTFQHSQKV AELCQHLAKE LGLNVEMQKA LYLTGLVHDI GKLHTPEEIL
310 320 330 340 350
HKPGKLNESE YLCIQRHSTD SRYTLQMVFG QSVVCEWAGN HHERLDGSGY
360 370 380 390 400
PRGLQGAAID LPSRIIAIAD VFQALTQARP YRGSMSLNEV MNIMRHEVSC
410 420 430
GRLDSQVFDV IVRNSQQYYQ LSIAESPTEW A
Length:431
Mass (Da):49,305
Last modified:October 1, 2000 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i16287AF1B5E037C4
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE003853 Genomic DNA Translation: AAF96581.1

Protein sequence database of the Protein Information Resource

More...
PIRi
G82430

NCBI Reference Sequences

More...
RefSeqi
NP_233069.1, NC_002506.1
WP_001882539.1, NC_002506.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAF96581; AAF96581; VC_A0681

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
2612204

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
vch:VCA0681

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|243277.26.peg.3306

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003853 Genomic DNA Translation: AAF96581.1
PIRiG82430
RefSeqiNP_233069.1, NC_002506.1
WP_001882539.1, NC_002506.1

3D structure databases

ProteinModelPortaliQ9KLR1
SMRiQ9KLR1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VCA0681

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
2612204
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF96581; AAF96581; VC_A0681
GeneIDi2612204
KEGGivch:VCA0681
PATRICifig|243277.26.peg.3306

Phylogenomic databases

eggNOGiENOG4105DHT Bacteria
COG2206 LUCA
OMAiHDCGVSS

Enzyme and pathway databases

BioCyciVCHO:VCA0681-MONOMER

Family and domain databases

CDDicd00077 HDc, 2 hits
InterProiView protein in InterPro
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR037522 HD_GYP_dom
IPR006675 HDIG_dom
PfamiView protein in Pfam
PF01966 HD, 2 hits
SMARTiView protein in SMART
SM00471 HDc, 2 hits
TIGRFAMsiTIGR00277 HDIG, 1 hit
PROSITEiView protein in PROSITE
PS51831 HD, 1 hit
PS51832 HD_GYP, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCGAP1_VIBCH
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9KLR1
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2016
Last sequence update: October 1, 2000
Last modified: December 20, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again