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UniProtKB - Q9KJ20 (GSDMT_ACTHA)
Protein
Glycine/sarcosine/dimethylglycine N-methyltransferase
Gene
N/A
Organism
Actinopolyspora halophila
Status
Functioni
Catalyzes the methylation of glycine, sarcosine and dimethylglycine to sarcosine, dimethylglycine and betaine, respectively, with S-adenosylmethionine (AdoMet) acting as the methyl donor.
1 PublicationCatalytic activityi
- glycine + 2 S-adenosyl-L-methionine = 2 H+ + N,N-dimethylglycine + 2 S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.1561 Publication
- 2 S-adenosyl-L-methionine + sarcosine = glycine betaine + 2 H+ + 2 S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.1571 Publication
: betaine biosynthesis via glycine pathway Pathwayi
This protein is involved in step 1, 2 and 3 of the subpathway that synthesizes betaine from glycine. This subpathway is part of the pathway betaine biosynthesis via glycine pathway, which is itself part of Amine and polyamine biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from glycine, the pathway betaine biosynthesis via glycine pathway and in Amine and polyamine biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 45 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 53 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 62 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 86 | S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Binding sitei | 107 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 152 | S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Binding sitei | 154 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 187 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 226 | SubstratePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- dimethylglycine N-methyltransferase activity Source: UniProtKB
- glycine N-methyltransferase activity Source: UniProtKB-EC
- sarcosine N-methyltransferase activity Source: UniProtKB
GO - Biological processi
- glycine betaine biosynthetic process from glycine Source: UniProtKB
- methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.156, 7834 |
UniPathwayi | UPA00530;UER00381 UPA00530;UER00382 UPA00530;UER00383 |
Names & Taxonomyi
Protein namesi | |
Organismi | Actinopolyspora halophila |
Taxonomic identifieri | 1850 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Actinopolysporales › Actinopolysporaceae › Actinopolyspora |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000413613 | 1 – 565 | Glycine/sarcosine/dimethylglycine N-methyltransferaseAdd BLAST | 565 |
Interactioni
Subunit structurei
Monomer.
1 PublicationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 34 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 134 – 135 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 2 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation
Family and domain databases
InterProi | View protein in InterPro IPR014369, Gly/Sar_N_MeTrfase IPR013216, Methyltransf_11 IPR041698, Methyltransf_25 IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF08241, Methyltransf_11, 1 hit PF13649, Methyltransf_25, 1 hit |
SUPFAMi | SSF53335, SSF53335, 2 hits |
PROSITEi | View protein in PROSITE PS51600, SAM_GNMT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9KJ20-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKSVDDLAR GDQAGDEQDP VHREQQTFGD NPLEVRDTDH YMHEYVGGFV
60 70 80 90 100
DKWDDLIDWK KRYESEGSFF IDQLRARGVE TVLDAAAGTG FHSVRLLEEG
110 120 130 140 150
FETVSADGSP QMLAKAFSNG LAYNGHILRV VNADWRWLNR DVHGEYDAII
160 170 180 190 200
CLGNSFTHLF SERDRRKTLA EFYAMLKHDG VLIIDQRNYD SILDTGFSSK
210 220 230 240 250
HTYYYAGEDV SAEPDHIDDG LARFKYTFPD KSEFFLNMYP LRKDYMRRLM
260 270 280 290 300
REVGFQRIDT YGDFQETYGE DEPDFYIHVA EKSYRTEDEF VDMYSNAVHT
310 320 330 340 350
ARDYYNSEDA DNFYYHVWGG NDIHVGLYQT PQEDIATASE RTVQRMAGKV
360 370 380 390 400
DISPETRILD LGAGYGGAAR YLARTYGCHV TCLNLSEVEN QRNREITRAE
410 420 430 440 450
GLEHLIEVTD GSFEDLPYQD NAFDVVWSQD SFLHSGDRSR VMEEVTRVLK
460 470 480 490 500
PKGSVLFTDP MASDSAKKNE LGPILDRLHL DSLGSPGFYR KELTRLGLQN
510 520 530 540 550
IEFEDLSEYL PVHYGRVLEV LESRENELAG FIGEEYRAHM KTGLRNWVQA
560
GNGGSLAWGI IHARA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF216283 Genomic DNA Translation: AAF87204.1 |
Genome annotation databases
KEGGi | ag:AAF87204 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF216283 Genomic DNA Translation: AAF87204.1 |
3D structure databases
SMRi | Q9KJ20 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:AAF87204 |
Enzyme and pathway databases
UniPathwayi | UPA00530;UER00381 UPA00530;UER00382 UPA00530;UER00383 |
BRENDAi | 2.1.1.156, 7834 |
Family and domain databases
InterProi | View protein in InterPro IPR014369, Gly/Sar_N_MeTrfase IPR013216, Methyltransf_11 IPR041698, Methyltransf_25 IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF08241, Methyltransf_11, 1 hit PF13649, Methyltransf_25, 1 hit |
SUPFAMi | SSF53335, SSF53335, 2 hits |
PROSITEi | View protein in PROSITE PS51600, SAM_GNMT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GSDMT_ACTHA | |
Accessioni | Q9KJ20Primary (citable) accession number: Q9KJ20 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2011 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 72 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families