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Protein

Ribonuclease H

Gene

rnhA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mn2+, Mg2+Note: Binds 2 metal ions per subunit. Manganese or magnesium.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi71Magnesium 11 Publication1
Metal bindingi71Magnesium 21 Publication1
Metal bindingi109Magnesium 21 Publication1
Metal bindingi132Magnesium 21 Publication1
Metal bindingi192Magnesium 11 Publication1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.26.4 661

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H (EC:3.1.26.4)
Short name:
RNase H
Gene namesi
Name:rnhA
Ordered Locus Names:BH0863
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi109E → Q: Loss of activity. 1 Publication1
Mutagenesisi132D → N: Loss of activity. 1 Publication1
Mutagenesisi188E → A: Strongly reduces activity. 1 Publication1
Mutagenesisi188E → Q: No effect. 1 Publication1
Mutagenesisi192D → N: Strongly reduced activity with manganese. Loss of activity with magnesium. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001954301 – 196Ribonuclease HAdd BLAST196

Interactioni

Protein-protein interaction databases

STRINGi272558.BH0863

Structurei

Secondary structure

1196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9KEI9
SMRiQ9KEI9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KEI9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 196RNase HAdd BLAST109

Sequence similaritiesi

Belongs to the RNase H family.Curated

Phylogenomic databases

eggNOGiENOG4108B1Q Bacteria
COG3341 LUCA
HOGENOMiHOG000251719
KOiK03469
OMAiFLAIVHG
OrthoDBiPOG091H04XT

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.40.970.10, 1 hit
InterProiView protein in InterPro
IPR009027 Ribosomal_L9/RNase_H1_N
IPR011320 RNase_H1_N
IPR037056 RNase_H1_N_sf
IPR017290 RNase_H_bac
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF01693 Cauli_VI, 1 hit
PIRSFiPIRSF037839 Ribonuclease_H, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
SSF55658 SSF55658, 1 hit

Sequencei

Sequence statusi: Complete.

Q9KEI9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKSKYYVVW NGRKPGIYTS WSACEAQVKG YTGAKFKSYP SKEEAEAAFR
60 70 80 90 100
GEEATPKLAK EEIIWESLSV DVGSQGNPGI VEYKGVDTKT GEVLFEREPI
110 120 130 140 150
PIGTNNMGEF LAIVHGLRYL KERNSRKPIY SDSQTAIKWV KDKKAKSTLV
160 170 180 190
RNEETALIWK LVDEAEEWLN THTYETPILK WQTDKWGEIK ADYGRK
Length:196
Mass (Da):22,373
Last modified:October 1, 2000 - v1
Checksum:i4130558FA37D2A86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA Translation: BAB04582.1
PIRiG83757
RefSeqiWP_010897036.1, NC_002570.2

Genome annotation databases

EnsemblBacteriaiBAB04582; BAB04582; BAB04582
KEGGibha:BH0863

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA Translation: BAB04582.1
PIRiG83757
RefSeqiWP_010897036.1, NC_002570.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZBFX-ray1.50A59-196[»]
1ZBIX-ray1.85A/B59-196[»]
1ZBLX-ray2.20A/B59-191[»]
2G8FX-ray1.65A59-196[»]
2G8HX-ray1.85A59-196[»]
2G8IX-ray1.65A59-196[»]
2G8KX-ray1.65A59-196[»]
2G8UX-ray2.70A59-196[»]
2G8VX-ray1.85A59-196[»]
2G8WX-ray2.05A59-196[»]
2R7YX-ray1.80A62-193[»]
3D0PX-ray1.80A/C61-194[»]
3EY1X-ray1.60A59-196[»]
3I8DX-ray1.61A/C62-193[»]
3TWHX-ray1.79A59-196[»]
3ULDX-ray1.60A59-196[»]
4HTUX-ray1.49A/B61-194[»]
4HUEX-ray1.56A/B61-194[»]
4HUFX-ray1.69A/B61-194[»]
4HUGX-ray1.64A/B61-194[»]
4OPJX-ray1.54A/C59-196[»]
4OPKX-ray1.54A/C59-196[»]
5SWMX-ray1.50A/B59-196[»]
5US2X-ray1.90A59-196[»]
5USAX-ray1.80A59-196[»]
5USEX-ray1.73A59-196[»]
5USGX-ray1.70A59-196[»]
5VAJX-ray1.95A/B59-196[»]
5W7NX-ray1.80A62-193[»]
5W7OX-ray1.75A62-193[»]
5WJRX-ray1.70A59-196[»]
6DMNX-ray1.27A59-196[»]
6DMVX-ray1.52A59-196[»]
6DO8X-ray1.41A59-196[»]
6DO9X-ray1.36A61-196[»]
6DOAX-ray1.47A61-196[»]
6DOBX-ray1.34A59-196[»]
6DOCX-ray1.50A59-196[»]
6DODX-ray1.53A59-196[»]
6DOEX-ray1.45A61-196[»]
6DOFX-ray1.43A59-196[»]
6DOGX-ray1.28A59-196[»]
6DOHX-ray1.36A61-193[»]
6DOIX-ray1.95A61-193[»]
6DOJX-ray1.40A61-195[»]
6DOKX-ray1.38A61-195[»]
6DOLX-ray1.43A61-195[»]
6DOMX-ray1.43A61-196[»]
6DONX-ray1.42A61-196[»]
6DOOX-ray1.44A61-196[»]
6DOPX-ray1.25A59-196[»]
6DOQX-ray1.42A59-196[»]
6DORX-ray1.50A61-196[»]
6DOSX-ray1.32A61-195[»]
6DOTX-ray1.42A61-196[»]
6DOUX-ray1.49A61-196[»]
6DOVX-ray1.52A61-196[»]
6DOWX-ray1.50A61-196[»]
6DOXX-ray1.45A61-196[»]
6DOYX-ray1.45A59-196[»]
6DOZX-ray1.57A59-196[»]
6DP0X-ray1.45A61-195[»]
6DP1X-ray1.42A59-196[»]
6DP2X-ray1.66A59-196[»]
6DP3X-ray1.46A59-196[»]
6DP4X-ray1.37A59-196[»]
6DP5X-ray1.43A59-196[»]
6DP6X-ray1.40A59-196[»]
6DP7X-ray1.38A61-195[»]
6DP8X-ray1.32A59-196[»]
6DP9X-ray1.40A61-196[»]
6DPAX-ray1.49A61-196[»]
6DPBX-ray1.32A61-196[»]
6DPCX-ray1.34A61-196[»]
6DPDX-ray1.46A61-196[»]
6DPEX-ray1.56A61-196[»]
6DPFX-ray1.56A61-196[»]
6DPGX-ray1.38A59-196[»]
6DPHX-ray1.34A59-196[»]
6DPIX-ray1.35A59-196[»]
6DPJX-ray1.55A59-196[»]
6DPKX-ray1.39A59-196[»]
6DPLX-ray1.45A59-196[»]
6DPMX-ray1.68A59-196[»]
6DPNX-ray1.49A59-196[»]
6DPOX-ray1.45A59-196[»]
6DPPX-ray1.45A61-196[»]
ProteinModelPortaliQ9KEI9
SMRiQ9KEI9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH0863

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB04582; BAB04582; BAB04582
KEGGibha:BH0863

Phylogenomic databases

eggNOGiENOG4108B1Q Bacteria
COG3341 LUCA
HOGENOMiHOG000251719
KOiK03469
OMAiFLAIVHG
OrthoDBiPOG091H04XT

Enzyme and pathway databases

BRENDAi3.1.26.4 661

Miscellaneous databases

EvolutionaryTraceiQ9KEI9

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.40.970.10, 1 hit
InterProiView protein in InterPro
IPR009027 Ribosomal_L9/RNase_H1_N
IPR011320 RNase_H1_N
IPR037056 RNase_H1_N_sf
IPR017290 RNase_H_bac
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF01693 Cauli_VI, 1 hit
PIRSFiPIRSF037839 Ribonuclease_H, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
SSF55658 SSF55658, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNH1_BACHD
AccessioniPrimary (citable) accession number: Q9KEI9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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