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Protein

Bifunctional enzyme BirA/CoaX

Gene

birA/coaX

Organism
Neisseria meningitidis serogroup B (strain MC58)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins.By similarity
Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.By similarity

Catalytic activityi

ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine.
ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate.

Cofactori

NH4(+)By similarity, K+By similarityNote: A monovalent cation. Ammonium or potassium.By similarity

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional enzyme BirA/CoaX (birA/coaX)
  2. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  3. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  4. Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei426SubstrateBy similarity1
Active sitei435Proton acceptorSequence analysis1
Binding sitei458ATPSequence analysis1
Binding sitei508SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi344 – 351ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Ligase, Multifunctional enzyme, Transferase
Biological processCoenzyme A biosynthesis
LigandATP-binding, Biotin, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciNMEN122586:G1G1B-2021-MONOMER
UniPathwayiUPA00241; UER00352

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enzyme BirA/CoaX
Including the following 2 domains:
Biotin--[acetyl-CoA-carboxylase] synthetase (EC:6.3.4.15)
Alternative name(s):
Biotin--protein ligase
Type III pantothenate kinase (EC:2.7.1.33)
Alternative name(s):
PanK-III
Pantothenic acid kinase
Gene namesi
Name:birA/coaX
Ordered Locus Names:NMB2075
OrganismiNeisseria meningitidis serogroup B (strain MC58)
Taxonomic identifieri122586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi
  • UP000000425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002708841 – 592Bifunctional enzyme BirA/CoaXAdd BLAST592

Proteomic databases

PaxDbiQ9JXF1

Interactioni

Protein-protein interaction databases

STRINGi122586.NMB2075

Structurei

3D structure databases

ProteinModelPortaliQ9JXF1
SMRiQ9JXF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 259BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST177

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 329Biotin--protein ligaseAdd BLAST329
Regioni336 – 592Type III pantothenate kinaseAdd BLAST257
Regioni433 – 436Substrate bindingBy similarity4

Sequence similaritiesi

In the N-terminal section; belongs to the biotin--protein ligase family.Curated
In the C-terminal section; belongs to the type III pantothenate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4108EKN Bacteria
COG0340 LUCA
COG1521 LUCA
HOGENOMiHOG000220772
KOiK01947
OMAiRPAGKRY
OrthoDBiPOG091H01NJ

Family and domain databases

CDDicd16442 BPL, 1 hit
HAMAPiMF_01274 Pantothen_kinase_3, 1 hit
InterProiView protein in InterPro
IPR004408 Biotin_CoA_COase_ligase
IPR003142 BPL_C
IPR004143 BPL_LPL_catalytic
IPR008988 Transcriptional_repressor_C
IPR004619 Type_III_PanK
PANTHERiPTHR12835:SF5 PTHR12835:SF5, 1 hit
PfamiView protein in Pfam
PF02237 BPL_C, 1 hit
PF03099 BPL_LplA_LipB, 1 hit
PF03309 Pan_kinase, 1 hit
SUPFAMiSSF50037 SSF50037, 1 hit
TIGRFAMsiTIGR00671 baf, 1 hit
TIGR00121 birA_ligase, 1 hit
PROSITEiView protein in PROSITE
PS51733 BPL_LPL_CATALYTIC, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JXF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVLKLSHWR VLAELADGLP QHVSQLARMA DMKPQQLNGF WQQMPAHIRG
60 70 80 90 100
LLRQHDGYWR LVRPLAVFDA EGLRELGERS GFQTALKHEC ASSNDEILEL
110 120 130 140 150
ARIAPDKAHK TICVTHLQSK GRGRQGRKWS HRLGECLMFS FGWVFDRPQY
160 170 180 190 200
ELGSLSPVAA VACRRALSRL GLDVQIKWPN DLVVGRDKLG GILIETVRTG
210 220 230 240 250
GKTVAVVGIG INFVLPKEVE NAASVQSLFQ TASRRGNADA AVLLETLLVE
260 270 280 290 300
LDAVLLQYAR DGFAPFVAEY QAANRDHGKA VLLLRDGETV FEGTVKGVDG
310 320 330 340 350
QGVLHLETAE GKQTVVSGEI SLRSDDRPVS VPKRRDSERF LLLDGGNSRL
360 370 380 390 400
KWAWVENGTF ATVGSAPYRD LSPLGAEWAE KADGNVRIVG CAVCGEFKKA
410 420 430 440 450
QVQEQLARKI EWLPSSAQAL GIRNHYRHPE EHGSDRWFNA LGSRRFSRNA
460 470 480 490 500
CVVVSCGTAV TVDALTDDGH YLGGTIMPGF HLMKESLAVR TANLNRHAGK
510 520 530 540 550
RYPFPTTTGN AVASGMMDAV CGSVMMMHGR LKEKTGAGKP VDVIITGGGA
560 570 580 590
AKVAEALPPA FLAENTVRVA DNLVIYGLLN MIAAEGREYE HI
Length:592
Mass (Da):64,701
Last modified:October 1, 2000 - v1
Checksum:i9E27421DA2B41DE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE002098 Genomic DNA Translation: AAF42394.1
PIRiB81009
RefSeqiNP_275065.1, NC_003112.2
WP_002225711.1, NC_003112.2

Genome annotation databases

EnsemblBacteriaiAAF42394; AAF42394; NMB2075
GeneIDi903989
KEGGinme:NMB2075
PATRICifig|122586.8.peg.2655

Similar proteinsi

Entry informationi

Entry nameiBICOA_NEIMB
AccessioniPrimary (citable) accession number: Q9JXF1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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