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Protein

Casein kinase I isoform epsilon

Gene

Csnk1e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Activity regulationi

Phosphorylation leads to a decrease in the catalytic activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 23ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processBiological rhythms, Wnt signaling pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 3474
ReactomeiR-MMU-201688 WNT mediated activation of DVL
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I isoform epsilon (EC:2.7.11.1)
Short name:
CKI-epsilon
Short name:
CKIe
Gene namesi
Name:Csnk1e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1351660 Csnk1e

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Has no apparent effect on circadian oscillation of protein levels. Mice exhibit a small but significant increase in circadian period length.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38K → A: Decreases PER1 phosphorylation. 1 Publication1
Mutagenesisi38K → R: Increases PER1 nuclear import. 1 Publication1
Mutagenesisi178R → C: Shortens circadian rhythm. Accelerates PER2 degradation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001928381 – 416Casein kinase I isoform epsilonAdd BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei343PhosphoserineBy similarity1
Modified residuei354PhosphoserineBy similarity1
Modified residuei362PhosphothreonineBy similarity1
Modified residuei363PhosphoserineBy similarity1
Modified residuei382Omega-N-methylarginineCombined sources1
Modified residuei389PhosphoserineBy similarity1
Modified residuei405PhosphoserineBy similarity1
Modified residuei408PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated. Partially dephosphorylated by PPP5C. May be dephosphorylated by PP1.

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ9JMK2
PaxDbiQ9JMK2
PeptideAtlasiQ9JMK2
PRIDEiQ9JMK2

PTM databases

iPTMnetiQ9JMK2
PhosphoSitePlusiQ9JMK2

Expressioni

Tissue specificityi

Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.1 Publication

Inductioni

Down-regulated during granulocytic differentiation. Does not show circadian oscillations.1 Publication

Gene expression databases

BgeeiENSMUSG00000022433 Expressed in 310 organ(s), highest expression level in cerebral cortex
CleanExiMM_CSNK1E
ExpressionAtlasiQ9JMK2 baseline and differential
GenevisibleiQ9JMK2 MM

Interactioni

Subunit structurei

Monomer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Interacts with ANKRD6, DBNDD2, LRP5, LRP6 and SOCS3. Interacts with SNAI1 (via zinc fingers). Interacts with DDX3X; the interaction greatly improves CSNK1E affinity for ATP and enhances DVL2 phosphorylation (By similarity).By similarity1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi205180, 49 interactors
DIPiDIP-32410N
IntActiQ9JMK2, 58 interactors
MINTiQ9JMK2
STRINGi10090.ENSMUSP00000113341

Structurei

3D structure databases

ProteinModelPortaliQ9JMK2
SMRiQ9JMK2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 277Protein kinasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1164 Eukaryota
ENOG410XPGP LUCA
GeneTreeiENSGT00920000148982
HOGENOMiHOG000182055
HOVERGENiHBG000176
InParanoidiQ9JMK2
KOiK08960
OrthoDBiEOG091G0AFG
PhylomeDBiQ9JMK2
TreeFamiTF300544

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.iShow all

Q9JMK2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELRVGNKYR LGRKIGSGSF GDIYLGANIA SGEEVAIKLE CVKTKHPQLH
60 70 80 90 100
IESKFYKMMQ GGVGIPSIKW CGAEGDYNVM VMELLGPSLE DLFNFCSRKF
110 120 130 140 150
SLKTVLLLAD QMISRIEYIH SKNFIHRDVK PDNFLMGLGK KGNLVYIIDF
160 170 180 190 200
GLAKKYRDAR THQHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG
210 220 230 240 250
YVLMYFNLGS LPWQGLKAAT KRQKYERISE KKMSTPIEVL CKGYPSEFST
260 270 280 290 300
YLNFCRSLRF DDKPDYSYLR QLFRNLFHRQ GFSYDYVFDW NMLKFGAARN
310 320 330 340 350
PEDVDRERRE HEREERMGQL RGSATRALPP GPPTGATANR LRSAAEPVAS
360 370 380 390 400
TPASRIQQTG NTSPRAISRA DRERKVSMRL HRGAPANVSS SDLTGRQEVS
410
RLAASQTSVP FDHLGK
Length:416
Mass (Da):47,322
Last modified:May 10, 2004 - v2
Checksum:i38CC5299BB9040D7
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TYE1Q3TYE1_MOUSE
Casein kinase I isoform epsilon
Csnk1e
368Annotation score:
A8Y2Z9A8Y2Z9_MOUSE
Casein kinase I isoform epsilon
Csnk1e
141Annotation score:
A8Y2Z5A8Y2Z5_MOUSE
Casein kinase I isoform epsilon
Csnk1e
118Annotation score:
A9J9D8A9J9D8_MOUSE
Casein kinase I isoform epsilon
Csnk1e
60Annotation score:
A0A2R8W6S2A0A2R8W6S2_MOUSE
Casein kinase I isoform epsilon
Csnk1e
188Annotation score:
A0A2R8VI54A0A2R8VI54_MOUSE
Casein kinase I isoform epsilon
Csnk1e
112Annotation score:
A0A2R8VHL1A0A2R8VHL1_MOUSE
Casein kinase I isoform epsilon
Csnk1e
55Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28N → D in BAA88107 (PubMed:10640823).Curated1
Sequence conflicti310E → G in BAA88107 (PubMed:10640823).Curated1
Sequence conflicti379R → G in BAA88107 (PubMed:10640823).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028736 mRNA Translation: BAA88107.2
BC026127 mRNA Translation: AAH26127.1
CCDSiCCDS27640.1
PIRiS47616
RefSeqiNP_001276827.1, NM_001289898.1
NP_038795.3, NM_013767.6
XP_017172146.1, XM_017316657.1
XP_017172147.1, XM_017316658.1
XP_017172148.1, XM_017316659.1
UniGeneiMm.30199

Genome annotation databases

EnsembliENSMUST00000117786; ENSMUSP00000113341; ENSMUSG00000022433
ENSMUST00000120859; ENSMUSP00000113975; ENSMUSG00000022433
GeneIDi27373
KEGGimmu:27373
UCSCiuc007wtl.2 mouse

Similar proteinsi

Entry informationi

Entry nameiKC1E_MOUSE
AccessioniPrimary (citable) accession number: Q9JMK2
Secondary accession number(s): Q8R389
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 10, 2004
Last modified: September 12, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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