UniProtKB - Q9JMH6 (TRXR1_MOUSE)
Protein
Thioredoxin reductase 1, cytoplasmic
Gene
Txnrd1
Organism
Mus musculus (Mouse)
Status
Functioni
Miscellaneous
The active site is a redox-active disulfide bond. The selenocysteine residue is also essential for catalytic activity (By similarity).By similarity
Catalytic activityi
- EC:1.8.1.9
Cofactori
FADBy similarityNote: Binds 1 FAD per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 586 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 156 – 173 | FADBy similarityAdd BLAST | 18 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: InterPro
- identical protein binding Source: MGI
- mercury ion binding Source: MGI
- NAD(P)H oxidase (H(2)O(2)-forming activity Source: MGI
- selenate reductase activity Source: MGI
- thioredoxin-disulfide reductase activity Source: MGI
GO - Biological processi
- benzene-containing compound metabolic process Source: MGI
- cell population proliferation Source: MGI
- cell redox homeostasis Source: GO_Central
- gastrulation Source: MGI
- hydrogen peroxide catabolic process Source: MGI
- mesoderm formation Source: MGI
- NADPH oxidation Source: MGI
- positive regulation of cell death Source: MGI
- response to oxidative stress Source: MGI
- selenocysteine metabolic process Source: MGI
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, NADP |
Enzyme and pathway databases
BRENDAi | 1.8.1.9, 3474 |
Reactomei | R-MMU-3299685, Detoxification of Reactive Oxygen Species R-MMU-499943, Interconversion of nucleotide di- and triphosphates R-MMU-5263617, Metabolism of ingested MeSeO2H into MeSeH R-MMU-5628897, TP53 Regulates Metabolic Genes |
Names & Taxonomyi
Protein namesi | Recommended name: Thioredoxin reductase 1, cytoplasmic (EC:1.8.1.9)Short name: TR Alternative name(s): Thioredoxin reductase TR1 |
Gene namesi | Name:Txnrd1 Synonyms:Trxr1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1354175, Txnrd1 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- fibrillar center Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- neuronal cell body Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000067982 | 1 – 613 | Thioredoxin reductase 1, cytoplasmicAdd BLAST | 613 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 173 ↔ 178 | Redox-activeBy similarity | ||
Modified residuei | 182 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 245 | PhosphotyrosineCombined sources | 1 | |
Cross-linki | 611 ↔ 612 | Cysteinyl-selenocysteine (Cys-Sec)By similarity |
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
EPDi | Q9JMH6 |
jPOSTi | Q9JMH6 |
PaxDbi | Q9JMH6 |
PeptideAtlasi | Q9JMH6 |
PRIDEi | Q9JMH6 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9JMH6 |
PTM databases
iPTMneti | Q9JMH6 |
PhosphoSitePlusi | Q9JMH6 |
SwissPalmi | Q9JMH6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020250, Expressed in cochlea and 317 other tissues |
Genevisiblei | Q9JMH6, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 206041, 10 interactors |
IntActi | Q9JMH6, 3 interactors |
MINTi | Q9JMH6 |
STRINGi | 10090.ENSMUSP00000020484 |
Miscellaneous databases
RNActi | Q9JMH6, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 26 – 90 | Pro-richAdd BLAST | 65 |
Sequence similaritiesi
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.Curated
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | KOG4716, Eukaryota |
GeneTreei | ENSGT00940000160180 |
HOGENOMi | CLU_016755_2_4_1 |
InParanoidi | Q9JMH6 |
OMAi | MHYAGNM |
OrthoDBi | 581771at2759 |
PhylomeDBi | Q9JMH6 |
TreeFami | TF314782 |
Family and domain databases
Gene3Di | 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR004099, Pyr_nucl-diS_OxRdtase_dimer IPR012999, Pyr_OxRdtase_I_AS IPR006338, Thioredoxin/glutathione_Rdtase |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit PF02852, Pyr_redox_dim, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit SSF55424, SSF55424, 1 hit |
TIGRFAMsi | TIGR01438, TGR, 1 hit |
PROSITEi | View protein in PROSITE PS00076, PYRIDINE_REDOX_1, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9JMH6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPVDDCWLYF PASRGRTFVQ TVWVAPTCPN CCWFPGFLPP VPRPPHVPRV
60 70 80 90 100
LLRGPRGAVL PASRPSKTLP SSSQTPCPTD PCICPPPSTP DSRQEKNTQS
110 120 130 140 150
ELPNKKGQLQ KLPTMNGSKD PPGSYDFDLI IIGGGSGGLA AAKEAAKFDK
160 170 180 190 200
KVLVLDFVTP TPLGTRWGLG GTCVNVGCIP KKLMHQAALL GQALKDSRNY
210 220 230 240 250
GWKVEDTVKH DWEKMTESVQ SHIGSLNWGY RVALREKKVV YENAYGRFIG
260 270 280 290 300
PHRIVATNNK GKEKIYSAER FLIATGERPR YLGIPGDKEY CISSDDLFSL
310 320 330 340 350
PYCPGKTLVV GASYVALECA GFLAGIGLDV TVMVRSILLR GFDQDMANKI
360 370 380 390 400
GEHMEEHGIK FIRQFVPTKI EQIEAGTPGR LRVTAQSTNS EETIEGEFNT
410 420 430 440 450
VLLAVGRDSC TRTIGLETVG VKINEKTGKI PVTDEEQTNV PYIYAIGDIL
460 470 480 490 500
EGKLELTPVA IQAGRLLAQR LYGGSNVKCD YDNVPTTVFT PLEYGCCGLS
510 520 530 540 550
EEKAVEKFGE ENIEVYHSFF WPLEWTVPSR DNNKCYAKII CNLKDDERVV
560 570 580 590 600
GFHVLGPNAG EVTQGFAAAL KCGLTKQQLD STIGIHPVCA EIFTTLSVTK
610
RSGGDILQSG CUG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P6U1 | A0A1W2P6U1_MOUSE | Thioredoxin reductase 1, cytoplasmi... | Txnrd1 | 164 | Annotation score: |
Sequence cautioni
The sequence AAH37643 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
The sequence BAE26918 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
The sequence BAE40292 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 238 | K → E in BAE28994 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 372 | Q → R in BAE26918 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 607 | L → V in BAE26918 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_031566 | 1 – 114 | Missing in isoform 2. 3 PublicationsAdd BLAST | 114 |
Non-standard residue
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-standard residuei | 612 | SelenocysteineBy similarity | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB027565 mRNA Translation: BAA86985.2 AF333036 mRNA Translation: AAK01140.1 AK011902 mRNA Translation: BAB27905.1 AK146125 mRNA Translation: BAE26918.1 Sequence problems. AK149625 mRNA Translation: BAE28994.1 AK168356 mRNA Translation: BAE40292.1 Sequence problems. BC037643 mRNA Translation: AAH37643.1 Sequence problems. |
CCDSi | CCDS24072.1 [Q9JMH6-2] CCDS88064.1 [Q9JMH6-1] |
RefSeqi | NP_001035978.1, NM_001042513.1 [Q9JMH6-2] NP_001035979.1, NM_001042514.1 [Q9JMH6-2] NP_001035988.1, NM_001042523.1 [Q9JMH6-1] NP_056577.2, NM_015762.2 [Q9JMH6-2] |
Genome annotation databases
Ensembli | ENSMUST00000020484; ENSMUSP00000020484; ENSMUSG00000020250 [Q9JMH6-2] ENSMUST00000219368; ENSMUSP00000151629; ENSMUSG00000020250 [Q9JMH6-1] ENSMUST00000219442; ENSMUSP00000152046; ENSMUSG00000020250 [Q9JMH6-2] ENSMUST00000219962; ENSMUSP00000151825; ENSMUSG00000020250 [Q9JMH6-2] |
GeneIDi | 50493 |
KEGGi | mmu:50493 |
UCSCi | uc007gjy.1, mouse [Q9JMH6-1] |
Keywords - Coding sequence diversityi
Alternative splicing, SelenocysteineSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB027565 mRNA Translation: BAA86985.2 AF333036 mRNA Translation: AAK01140.1 AK011902 mRNA Translation: BAB27905.1 AK146125 mRNA Translation: BAE26918.1 Sequence problems. AK149625 mRNA Translation: BAE28994.1 AK168356 mRNA Translation: BAE40292.1 Sequence problems. BC037643 mRNA Translation: AAH37643.1 Sequence problems. |
CCDSi | CCDS24072.1 [Q9JMH6-2] CCDS88064.1 [Q9JMH6-1] |
RefSeqi | NP_001035978.1, NM_001042513.1 [Q9JMH6-2] NP_001035979.1, NM_001042514.1 [Q9JMH6-2] NP_001035988.1, NM_001042523.1 [Q9JMH6-1] NP_056577.2, NM_015762.2 [Q9JMH6-2] |
3D structure databases
SMRi | Q9JMH6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206041, 10 interactors |
IntActi | Q9JMH6, 3 interactors |
MINTi | Q9JMH6 |
STRINGi | 10090.ENSMUSP00000020484 |
PTM databases
iPTMneti | Q9JMH6 |
PhosphoSitePlusi | Q9JMH6 |
SwissPalmi | Q9JMH6 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9JMH6 |
Proteomic databases
EPDi | Q9JMH6 |
jPOSTi | Q9JMH6 |
PaxDbi | Q9JMH6 |
PeptideAtlasi | Q9JMH6 |
PRIDEi | Q9JMH6 |
Protocols and materials databases
Antibodypediai | 3897, 435 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000020484; ENSMUSP00000020484; ENSMUSG00000020250 [Q9JMH6-2] ENSMUST00000219368; ENSMUSP00000151629; ENSMUSG00000020250 [Q9JMH6-1] ENSMUST00000219442; ENSMUSP00000152046; ENSMUSG00000020250 [Q9JMH6-2] ENSMUST00000219962; ENSMUSP00000151825; ENSMUSG00000020250 [Q9JMH6-2] |
GeneIDi | 50493 |
KEGGi | mmu:50493 |
UCSCi | uc007gjy.1, mouse [Q9JMH6-1] |
Organism-specific databases
CTDi | 7296 |
MGIi | MGI:1354175, Txnrd1 |
Phylogenomic databases
eggNOGi | KOG4716, Eukaryota |
GeneTreei | ENSGT00940000160180 |
HOGENOMi | CLU_016755_2_4_1 |
InParanoidi | Q9JMH6 |
OMAi | MHYAGNM |
OrthoDBi | 581771at2759 |
PhylomeDBi | Q9JMH6 |
TreeFami | TF314782 |
Enzyme and pathway databases
BRENDAi | 1.8.1.9, 3474 |
Reactomei | R-MMU-3299685, Detoxification of Reactive Oxygen Species R-MMU-499943, Interconversion of nucleotide di- and triphosphates R-MMU-5263617, Metabolism of ingested MeSeO2H into MeSeH R-MMU-5628897, TP53 Regulates Metabolic Genes |
Miscellaneous databases
BioGRID-ORCSi | 50493, 6 hits in 17 CRISPR screens |
ChiTaRSi | Txnrd1, mouse |
PROi | PR:Q9JMH6 |
RNActi | Q9JMH6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020250, Expressed in cochlea and 317 other tissues |
Genevisiblei | Q9JMH6, MM |
Family and domain databases
Gene3Di | 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR004099, Pyr_nucl-diS_OxRdtase_dimer IPR012999, Pyr_OxRdtase_I_AS IPR006338, Thioredoxin/glutathione_Rdtase |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit PF02852, Pyr_redox_dim, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit SSF55424, SSF55424, 1 hit |
TIGRFAMsi | TIGR01438, TGR, 1 hit |
PROSITEi | View protein in PROSITE PS00076, PYRIDINE_REDOX_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRXR1_MOUSE | |
Accessioni | Q9JMH6Primary (citable) accession number: Q9JMH6 Secondary accession number(s): Q3UEB7 Q9CSV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2002 |
Last sequence update: | February 26, 2008 | |
Last modified: | December 2, 2020 | |
This is version 185 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families