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Protein

Endothelial differentiation-related factor 1

Gene

Edf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. Might function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi92 – 111H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Calmodulin-binding, Developmental protein, DNA-binding
Biological processDifferentiation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelial differentiation-related factor 1
Short name:
EDF-1
Alternative name(s):
Multiprotein-bridging factor 1
Short name:
MBF1
Gene namesi
Name:Edf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1891227 Edf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001497962 – 148Endothelial differentiation-related factor 1Add BLAST147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei25N6-methyllysineBy similarity1

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9JMG1
MaxQBiQ9JMG1
PaxDbiQ9JMG1
PeptideAtlasiQ9JMG1
PRIDEiQ9JMG1

PTM databases

iPTMnetiQ9JMG1
PhosphoSitePlusiQ9JMG1

Expressioni

Tissue specificityi

Expressed in brain, liver, kidney and heart (at protein level). Also expressed in testis.1 Publication

Developmental stagei

Very highly expressed seven days after implantation, when gastrulation and neurulation occurs. Expression decreases 11 days after implantation, when organogenesis is being completed, and remains rather low up to late developmental stages.1 Publication

Gene expression databases

BgeeiENSMUSG00000015092 Expressed in 292 organ(s), highest expression level in paneth cell
CleanExiMM_EDF1
GenevisibleiQ9JMG1 MM

Interactioni

Subunit structurei

Interacts with TBP and the transcription factor IID (TFIID) complex, NR5A2, NR1H3 and PPARG. Interaction with TBP is regulated by phosphorylation. Binds NR5A1, ATF1, FOS and JUN via their conserved basic region. Binding to calmodulin is regulated by calcium and phosphorylation of the IQ motif (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015236

Structurei

3D structure databases

ProteinModelPortaliQ9JMG1
SMRiQ9JMG1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 135HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 113Interaction with NR5A2, PPARG and NR1H3By similarityAdd BLAST77
Regioni69 – 108Interaction with TBP and NR5A1By similarityAdd BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi81 – 88IQ motifBy similarity8

Domaini

The IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein (By similarity).By similarity

Phylogenomic databases

eggNOGiKOG3398 Eukaryota
COG1813 LUCA
GeneTreeiENSGT00390000008519
HOGENOMiHOG000195726
HOVERGENiHBG051440
InParanoidiQ9JMG1
KOiK03627
OMAiIADYECG
OrthoDBiEOG091G0RRN
PhylomeDBiQ9JMG1
TreeFamiTF300064

Family and domain databases

CDDicd00093 HTH_XRE, 1 hit
InterProiView protein in InterPro
IPR001387 Cro/C1-type_HTH
IPR010982 Lambda_DNA-bd_dom_sf
IPR013729 MBF1_N
PfamiView protein in Pfam
PF01381 HTH_3, 1 hit
PF08523 MBF1, 1 hit
SMARTiView protein in SMART
SM00530 HTH_XRE, 1 hit
SUPFAMiSSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS50943 HTH_CROC1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JMG1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK
60 70 80 90 100
QHSITKNTAK LDRETEELHH DRVTLEVGKV IQRGRQSKGL TQKDLATKIN
110 120 130 140
EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPKAK
Length:148
Mass (Da):16,369
Last modified:October 1, 2000 - v1
Checksum:iADC000D8492A8C11
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146K → R in CAC32040 (PubMed:11587857).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030185 mRNA Translation: BAA92749.1
AJ309569 mRNA Translation: CAC32040.1
AK002345 mRNA Translation: BAB22026.1
AK003557 mRNA Translation: BAB22854.1
AK010191 mRNA Translation: BAB26758.1
BC023472 mRNA Translation: AAH23472.1
CCDSiCCDS15780.1
RefSeqiNP_067494.1, NM_021519.1
UniGeneiMm.29945

Genome annotation databases

EnsembliENSMUST00000015236; ENSMUSP00000015236; ENSMUSG00000015092
GeneIDi59022
KEGGimmu:59022
UCSCiuc008isn.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030185 mRNA Translation: BAA92749.1
AJ309569 mRNA Translation: CAC32040.1
AK002345 mRNA Translation: BAB22026.1
AK003557 mRNA Translation: BAB22854.1
AK010191 mRNA Translation: BAB26758.1
BC023472 mRNA Translation: AAH23472.1
CCDSiCCDS15780.1
RefSeqiNP_067494.1, NM_021519.1
UniGeneiMm.29945

3D structure databases

ProteinModelPortaliQ9JMG1
SMRiQ9JMG1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015236

PTM databases

iPTMnetiQ9JMG1
PhosphoSitePlusiQ9JMG1

Proteomic databases

EPDiQ9JMG1
MaxQBiQ9JMG1
PaxDbiQ9JMG1
PeptideAtlasiQ9JMG1
PRIDEiQ9JMG1

Protocols and materials databases

DNASUi59022
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015236; ENSMUSP00000015236; ENSMUSG00000015092
GeneIDi59022
KEGGimmu:59022
UCSCiuc008isn.1 mouse

Organism-specific databases

CTDi8721
MGIiMGI:1891227 Edf1

Phylogenomic databases

eggNOGiKOG3398 Eukaryota
COG1813 LUCA
GeneTreeiENSGT00390000008519
HOGENOMiHOG000195726
HOVERGENiHBG051440
InParanoidiQ9JMG1
KOiK03627
OMAiIADYECG
OrthoDBiEOG091G0RRN
PhylomeDBiQ9JMG1
TreeFamiTF300064

Miscellaneous databases

ChiTaRSiEdf1 mouse
PROiPR:Q9JMG1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015092 Expressed in 292 organ(s), highest expression level in paneth cell
CleanExiMM_EDF1
GenevisibleiQ9JMG1 MM

Family and domain databases

CDDicd00093 HTH_XRE, 1 hit
InterProiView protein in InterPro
IPR001387 Cro/C1-type_HTH
IPR010982 Lambda_DNA-bd_dom_sf
IPR013729 MBF1_N
PfamiView protein in Pfam
PF01381 HTH_3, 1 hit
PF08523 MBF1, 1 hit
SMARTiView protein in SMART
SM00530 HTH_XRE, 1 hit
SUPFAMiSSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS50943 HTH_CROC1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEDF1_MOUSE
AccessioniPrimary (citable) accession number: Q9JMG1
Secondary accession number(s): Q99NE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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