UniProtKB - Q9JMD7 (SC5A7_RAT)
Protein
High affinity choline transporter 1
Gene
Slc5a7
Organism
Rattus norvegicus (Rat)
Status
Functioni
Transmembrane transporter that imports choline from the extracellular space to the neuron with high affinity. Choline uptake is the rate-limiting step in acetylcholine synthesis. Sodium ion- and chloride ion-dependent.1 Publication
Miscellaneous
Specifically inhibited by nanomolar concentrations of hemicholinium 3.
GO - Molecular functioni
- choline:sodium symporter activity Source: RGD
- choline binding Source: RGD
- choline transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- acetylcholine biosynthetic process Source: UniProtKB
- choline transport Source: RGD
- neuromuscular synaptic transmission Source: RGD
- synaptic transmission, cholinergic Source: RGD
Keywordsi
Biological process | Ion transport, Neurotransmitter biosynthesis, Sodium transport, Symport, Transport |
Ligand | Sodium |
Enzyme and pathway databases
Reactomei | R-RNO-264642, Acetylcholine Neurotransmitter Release Cycle R-RNO-425366, Transport of bile salts and organic acids, metal ions and amine compounds |
Protein family/group databases
TCDBi | 2.A.21.8.1, the solute:sodium symporter (sss) family |
Names & Taxonomyi
Protein namesi | Recommended name: High affinity choline transporter 1Alternative name(s): Hemicholinium-3-sensitive choline transporter Short name: CHT Solute carrier family 5 member 7 |
Gene namesi | Name:Slc5a7 Synonyms:Cht1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 69270, Slc5a7 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Other locations
- Membrane By similarity; Multi-pass membrane protein By similarity
- synapse By similarity
Note: Localized at the neuromuscular junction.By similarity
Endosome
- early endosome Source: RGD
Plasma Membrane
- apical plasma membrane Source: RGD
- plasma membrane Source: RGD
- plasma membrane raft Source: RGD
Other locations
- axon Source: RGD
- clathrin-coated endocytic vesicle Source: RGD
- dendrite Source: RGD
- integral component of membrane Source: RGD
- neuromuscular junction Source: RGD
- neuronal cell body Source: RGD
- perikaryon Source: RGD
- synapse Source: RGD
- synaptic vesicle Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | ExtracellularSequence analysis | 6 | |
Transmembranei | 7 – 27 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 28 – 48 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 49 – 69 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 70 – 81 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 82 – 102 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 103 – 125 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 126 – 146 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 147 – 164 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 165 – 185 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 186 – 191 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 192 – 212 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 213 – 237 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 238 – 258 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 259 – 274 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 275 – 295 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 296 – 317 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 318 – 338 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 339 – 376 | CytoplasmicSequence analysisAdd BLAST | 38 | |
Transmembranei | 377 – 397 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 398 – 406 | ExtracellularSequence analysis | 9 | |
Transmembranei | 407 – 427 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 428 – 435 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 436 – 456 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 457 – 481 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 482 – 502 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 503 – 580 | CytoplasmicSequence analysisAdd BLAST | 78 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105393 | 1 – 580 | High affinity choline transporter 1Add BLAST | 580 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 301 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Phosphorylated.By similarity
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q9JMD7 |
PRIDEi | Q9JMD7 |
PTM databases
GlyGeni | Q9JMD7, 1 site |
iPTMneti | Q9JMD7 |
PhosphoSitePlusi | Q9JMD7 |
Expressioni
Tissue specificityi
Expressed in basal forebrain, brain stem, spinal chord, and striatum. Specific for cholinergic neurons.1 Publication
Interactioni
Subunit structurei
Homooligomerizes at cell surface (By similarity).
Interacts with SEC14L1; may regulate SLC5A7 (PubMed:17092608).
By similarity1 PublicationProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000014548 |
Chemistry databases
BindingDBi | Q9JMD7 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 502 – 580 | Mediates interaction with SEC14L11 PublicationAdd BLAST | 79 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3761, Eukaryota |
InParanoidi | Q9JMD7 |
PhylomeDBi | Q9JMD7 |
Family and domain databases
Gene3Di | 1.20.1730.10, 1 hit |
InterProi | View protein in InterPro IPR038377, Na/Glc_symporter_sf IPR001734, Na/solute_symporter |
Pfami | View protein in Pfam PF00474, SSF, 1 hit |
PROSITEi | View protein in PROSITE PS50283, NA_SOLUT_SYMP_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9JMD7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPFHVEGLVA IILFYLLIFL VGIWAAWKTK NSGNAEERSE AIIVGGRDIG
60 70 80 90 100
LLVGGFTMTA TWVGGGYING TAEAVYGPGC GLAWAQAPIG YSLSLILGGL
110 120 130 140 150
FFAKPMRSKG YVTMLDPFQQ IYGKRMGGLL FIPALMGEMF WAAAIFSALG
160 170 180 190 200
ATISVIIDVD VNISVIVSAL IAILYTLVGG LYSVAYTDVV QLFCIFIGLW
210 220 230 240 250
ISVPFALSHP AVTDIGFTAV HAKYQSPWLG TIESVEVYTW LDNFLLLMLG
260 270 280 290 300
GIPWQAYFQR VLSSSSATYA QVLSFLAAFG CLVMALPAIC IGAIGASTDW
310 320 330 340 350
NQTAYGFPDP KTKEEADMIL PIVLQYLCPV YISFFGLGAV SAAVMSSADS
360 370 380 390 400
SILSASSMFA RNIYQLSFRQ NASDKEIVWV MRITVFVFGA SATAMALLTK
410 420 430 440 450
TVYGLWYLSS DLVYIIIFPQ LLCVLFIKGT NTYGAVAGYI FGLFLRITGG
460 470 480 490 500
EPYLYLQPLI FYPGYYPDKN GIYNQRFPFK TLSMVTSFFT NICVSYLAKY
510 520 530 540 550
LFESGTLPPK LDIFDAVVSR HSEENMDKTI LVRNENIKLN ELAPVKPRQS
560 570 580
LTLSSTFTNK EALLDVDSSP EGSGTEDNLQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V7G1 | G3V7G1_RAT | High affinity choline transporter 1 | Slc5a7 rCG_53387 | 580 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB030947 mRNA Translation: BAA90484.1 |
RefSeqi | NP_445973.1, NM_053521.1 |
Genome annotation databases
GeneIDi | 85426 |
KEGGi | rno:85426 |
UCSCi | RGD:69270, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB030947 mRNA Translation: BAA90484.1 |
RefSeqi | NP_445973.1, NM_053521.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000014548 |
Chemistry databases
BindingDBi | Q9JMD7 |
ChEMBLi | CHEMBL5797 |
Protein family/group databases
TCDBi | 2.A.21.8.1, the solute:sodium symporter (sss) family |
PTM databases
GlyGeni | Q9JMD7, 1 site |
iPTMneti | Q9JMD7 |
PhosphoSitePlusi | Q9JMD7 |
Proteomic databases
PaxDbi | Q9JMD7 |
PRIDEi | Q9JMD7 |
Genome annotation databases
GeneIDi | 85426 |
KEGGi | rno:85426 |
UCSCi | RGD:69270, rat |
Organism-specific databases
CTDi | 60482 |
RGDi | 69270, Slc5a7 |
Phylogenomic databases
eggNOGi | KOG3761, Eukaryota |
InParanoidi | Q9JMD7 |
PhylomeDBi | Q9JMD7 |
Enzyme and pathway databases
Reactomei | R-RNO-264642, Acetylcholine Neurotransmitter Release Cycle R-RNO-425366, Transport of bile salts and organic acids, metal ions and amine compounds |
Miscellaneous databases
PROi | PR:Q9JMD7 |
Family and domain databases
Gene3Di | 1.20.1730.10, 1 hit |
InterProi | View protein in InterPro IPR038377, Na/Glc_symporter_sf IPR001734, Na/solute_symporter |
Pfami | View protein in Pfam PF00474, SSF, 1 hit |
PROSITEi | View protein in PROSITE PS50283, NA_SOLUT_SYMP_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SC5A7_RAT | |
Accessioni | Q9JMD7Primary (citable) accession number: Q9JMD7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 7, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families