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Protein

Bifunctional polynucleotide phosphatase/kinase

Gene

Pnkp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.

Catalytic activityi

A 3'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi371 – 378ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Kinase, Multifunctional enzyme, Transferase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.78 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional polynucleotide phosphatase/kinase
Alternative name(s):
DNA 5'-kinase/3'-phosphatase
Polynucleotide kinase-3'-phosphatase
Including the following 2 domains:
Polynucleotide 3'-phosphatase (EC:3.1.3.32)
Alternative name(s):
2'(3')-polynucleotidase
Polynucleotide 5'-hydroxyl-kinase (EC:2.7.1.78)
Gene namesi
Name:Pnkp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1891698 Pnkp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44R → A: Abrogates phosphopeptide recognition. 1 Publication1
Mutagenesisi45K → N: No effect on phosphopeptide recognition. 1 Publication1
Mutagenesisi48R → A: Abrogates phosphopeptide recognition. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000584791 – 522Bifunctional polynucleotide phosphatase/kinaseAdd BLAST522

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei114PhosphoserineBy similarity1
Modified residuei122PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JLV6
PaxDbiQ9JLV6
PeptideAtlasiQ9JLV6
PRIDEiQ9JLV6

PTM databases

iPTMnetiQ9JLV6
PhosphoSitePlusiQ9JLV6

Expressioni

Gene expression databases

CleanExiMM_PNKP

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiQ9JLV6, 2 interactors
MINTiQ9JLV6
STRINGi10090.ENSMUSP00000003044

Structurei

Secondary structure

1522
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9JLV6
SMRiQ9JLV6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JLV6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 110FHAAdd BLAST105

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni145 – 336PhosphataseAdd BLAST192
Regioni340 – 517KinaseAdd BLAST178

Domaini

The FHA domain binds threonine-phosphorylated peptides from XRCC1/4, and is responsible for the recruitment of PNKP to the sites of DNA repair. The affinity is ten times greater if peptides are also phosphorylated on the serine preceeding the phosphothreonine.1 Publication

Sequence similaritiesi

In the N-terminal section; belongs to the DNA 3' phosphatase family.Curated

Phylogenomic databases

eggNOGiKOG2134 Eukaryota
COG0241 LUCA
HOGENOMiHOG000031466
HOVERGENiHBG053624
InParanoidiQ9JLV6
PhylomeDBiQ9JLV6

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR006549 HAD-SF_hydro_IIIA
IPR023214 HAD_sf
IPR027417 P-loop_NTPase
IPR013954 PNK3P
IPR006550 PNK_3Pase_met
IPR006551 Polynucleotide_phosphatase
IPR008984 SMAD_FHA_dom_sf
PfamiView protein in Pfam
PF08645 PNK3P, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
SSF52540 SSF52540, 1 hit
SSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01664 DNA-3'-Pase, 1 hit
TIGR01662 HAD-SF-IIIA, 1 hit
TIGR01663 PNK-3'Pase, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 11 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q9JLV6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQLGSRGRL WLQSPTGGPP PIFLPSDGQA LVLGRGPLTQ VTDRKCSRNQ
60 70 80 90 100
VELIADPESR TVAVKQLGVN PSTVGVHELK PGLSGSLSLG DVLYLVNGLY
110 120 130 140 150
PLTLRWEELS TSGSQPDAPP DTPGDPEEGE DTEPQKKRVR KSSLGWESLK
160 170 180 190 200
KLLVFTASGV KPQGKVAAFD LDGTLITTRS GKVFPTSPSD WRILYPEIPK
210 220 230 240 250
KLQELAAEGY KLVIFTNQMG IGRGKLPAEV FKGKVEAVLE KLGVPFQVLV
260 270 280 290 300
ATHAGLNRKP VSGMWDHLQE QANEGIPISV EDSVFVGDAA GRLANWAPGR
310 320 330 340 350
KKKDFSCADR LFALNVGLPF ATPEEFFLKW PAARFELPAF DPRTISSAGP
360 370 380 390 400
LYLPESSSLL SPNPEVVVAV GFPGAGKSTF IQEHLVSAGY VHVNRDTLGS
410 420 430 440 450
WQRCVSSCQA ALRQGKRVVI DNTNPDVPSR ARYIQCAKDA GVPCRCFNFC
460 470 480 490 500
ATIEQARHNN RFREMTDPSH APVSDMVMFS YRKQFEPPTL AEGFLEILEI
510 520
PFRLQEHLDP ALQRLYRQFS EG
Length:522
Mass (Da):57,223
Last modified:July 19, 2004 - v2
Checksum:iC691C0A6DA9F47FE
GO
Isoform 2 (identifier: Q9JLV6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     163-191: QGKVAAFDLDGTLITTRSGKVFPTSPSDW → RA

Note: No experimental confirmation available.
Show »
Length:495
Mass (Da):54,373
Checksum:i6FE381B1531C953C
GO

Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G5E8N7G5E8N7_MOUSE
Bifunctional polynucleotide phospha...
Pnkp mCG_23134
522Annotation score:
E9Q9A5E9Q9A5_MOUSE
Bifunctional polynucleotide phospha...
Pnkp
486Annotation score:
A0A0J9YVJ6A0A0J9YVJ6_MOUSE
Bifunctional polynucleotide phospha...
Pnkp
426Annotation score:
A0A140LIZ1A0A140LIZ1_MOUSE
Bifunctional polynucleotide phospha...
Pnkp
255Annotation score:
A0A0J9YV48A0A0J9YV48_MOUSE
Bifunctional polynucleotide phospha...
Pnkp
148Annotation score:
A0A0J9YVH5A0A0J9YVH5_MOUSE
Bifunctional polynucleotide phospha...
Pnkp
59Annotation score:
A0A0J9YUF9A0A0J9YUF9_MOUSE
Bifunctional polynucleotide phospha...
Pnkp
151Annotation score:
A0A140LJ53A0A140LJ53_MOUSE
Bifunctional polynucleotide phospha...
Pnkp
80Annotation score:
A0A0J9YTV1A0A0J9YTV1_MOUSE
Bifunctional polynucleotide phospha...
Pnkp
180Annotation score:
D3YZV7D3YZV7_MOUSE
Bifunctional polynucleotide phospha...
Pnkp
34Annotation score:
There is more potential isoformShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77H → Q in AAF36487 (Ref. 1) Curated1
Sequence conflicti216Missing in AAF36487 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010771163 – 191QGKVA…SPSDW → RA in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129451 mRNA Translation: AAF36487.1
BC057659 mRNA Translation: AAH57659.1
CCDSiCCDS39945.1 [Q9JLV6-1]
UniGeneiMm.238254
Mm.487968

Genome annotation databases

UCSCiuc012fjx.1 mouse [Q9JLV6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPNKP_MOUSE
AccessioniPrimary (citable) accession number: Q9JLV6
Secondary accession number(s): Q6PFA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: September 12, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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