UniProtKB - Q9JLV1 (BAG3_MOUSE)
Protein
BAG family molecular chaperone regulator 3
Gene
Bag3
Organism
Mus musculus (Mouse)
Status
Functioni
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.By similarity
GO - Molecular functioni
- adenyl-nucleotide exchange factor activity Source: UniProtKB
- chaperone binding Source: MGI
- heat shock protein binding Source: GO_Central
- protein-containing complex binding Source: MGI
GO - Biological processi
- autophagosome assembly Source: MGI
- brain development Source: Ensembl
- cellular response to heat Source: UniProtKB
- cellular response to mechanical stimulus Source: MGI
- cellular response to unfolded protein Source: MGI
- chaperone-mediated autophagy Source: MGI
- extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
- muscle cell cellular homeostasis Source: BHF-UCL
- negative regulation of apoptotic process Source: MGI
- negative regulation of protein targeting to mitochondrion Source: MGI
- negative regulation of striated muscle cell apoptotic process Source: MGI
- negative regulation of transcription from RNA polymerase II promoter in response to stress Source: UniProtKB
- positive regulation of aggrephagy Source: MGI
- positive regulation of protein export from nucleus Source: UniProtKB
- positive regulation of protein import into nucleus Source: UniProtKB
- protein stabilization Source: MGI
- spinal cord development Source: Ensembl
Keywordsi
Molecular function | Chaperone |
Biological process | Apoptosis |
Enzyme and pathway databases
Reactomei | R-MMU-3371453, Regulation of HSF1-mediated heat shock response |
Names & Taxonomyi
Protein namesi | Recommended name: BAG family molecular chaperone regulator 3Short name: BAG-3 Alternative name(s): Bcl-2-associated athanogene 3 Bcl-2-binding protein Bis |
Gene namesi | Name:Bag3 Synonyms:Bis ORF Names:MNCb-2243 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1352493, Bag3 |
Subcellular locationi
Cytoskeleton
- stress fiber Source: MGI
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- chaperone complex Source: MGI
- cytoplasm Source: UniProtKB
- membrane Source: GO_Central
- neuron projection Source: MGI
- Z disc Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000088869 | 2 – 577 | BAG family molecular chaperone regulator 3Add BLAST | 576 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 138 | PhosphoserineCombined sources | 1 | |
Modified residuei | 141 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 179 | PhosphoserineBy similarity | 1 | |
Modified residuei | 204 | PhosphoserineBy similarity | 1 | |
Modified residuei | 267 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 280 | PhosphoserineBy similarity | 1 | |
Modified residuei | 281 | PhosphoserineBy similarity | 1 | |
Modified residuei | 285 | PhosphoserineBy similarity | 1 | |
Modified residuei | 291 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 297 | PhosphoserineCombined sources | 1 | |
Modified residuei | 380 | PhosphoserineCombined sources | 1 | |
Modified residuei | 382 | PhosphoserineCombined sources | 1 | |
Modified residuei | 390 | PhosphoserineCombined sources | 1 | |
Cross-linki | 450 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 450 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity |
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q9JLV1 |
MaxQBi | Q9JLV1 |
PaxDbi | Q9JLV1 |
PRIDEi | Q9JLV1 |
PTM databases
iPTMneti | Q9JLV1 |
PhosphoSitePlusi | Q9JLV1 |
SwissPalmi | Q9JLV1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000030847, Expressed in cardiac muscle of left ventricle and 262 other tissues |
Genevisiblei | Q9JLV1, MM |
Interactioni
Subunit structurei
Binds to the ATPase domain of HSP70/HSC70 chaperones.
Interacts with BCL2.
Interacts with phospholipase C-gamma proteins.
Interacts with DNAJB6.
Interacts (via BAG domain) with HSF1; this interaction occurs in normal and heat-shocked cells.
Interacts with HSPA8 (via NBD), HSPA1A (via NBD) and HSPA1B (via NBD).
Interacts (via WW domain 1) with SYNPO2 (via PPPY motif).
By similarityBinary interactionsi
Q9JLV1
With | #Exp. | IntAct |
---|---|---|
EBNA-LP [Q8AZK7] from Epstein-Barr virus (strain B95-8). | 2 | EBI-309231,EBI-1185167 |
GO - Molecular functioni
- chaperone binding Source: MGI
- heat shock protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 205891, 70 interactors |
IntActi | Q9JLV1, 6 interactors |
MINTi | Q9JLV1 |
STRINGi | 10090.ENSMUSP00000033136 |
Miscellaneous databases
RNActi | Q9JLV1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9JLV1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9JLV1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 56 | WW 1PROSITE-ProRule annotationAdd BLAST | 35 | |
Domaini | 126 – 157 | WW 2PROSITE-ProRule annotationAdd BLAST | 32 | |
Domaini | 426 – 503 | BAGPROSITE-ProRule annotationAdd BLAST | 78 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 186 – 193 | Poly-Ser | 8 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0940, Eukaryota KOG4361, Eukaryota |
GeneTreei | ENSGT00940000159204 |
HOGENOMi | CLU_034378_0_0_1 |
InParanoidi | Q9JLV1 |
OMAi | THSPMMQ |
OrthoDBi | 715914at2759 |
TreeFami | TF102013 |
Family and domain databases
CDDi | cd00201, WW, 1 hit |
Gene3Di | 1.20.58.120, 1 hit |
InterProi | View protein in InterPro IPR039773, BAG_chaperone_regulator IPR036533, BAG_dom_sf IPR003103, BAG_domain IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR12329, PTHR12329, 1 hit |
Pfami | View protein in Pfam PF02179, BAG, 1 hit PF00397, WW, 1 hit |
SMARTi | View protein in SMART SM00264, BAG, 1 hit SM00456, WW, 1 hit |
SUPFAMi | SSF51045, SSF51045, 1 hit SSF63491, SSF63491, 1 hit |
PROSITEi | View protein in PROSITE PS51035, BAG, 1 hit PS01159, WW_DOMAIN_1, 1 hit PS50020, WW_DOMAIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9JLV1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAATQSPMM QMASGNGASD RDPLPPGWEI KIDPQTGWPF FVDHNSRTTT
60 70 80 90 100
WNDPRVPPEG PKDTASSANG PSRDGSRLLP IREGHPIYPQ LRPGYIPIPV
110 120 130 140 150
LHEGSENRQP HLFHAYSQPG VQRFRTEAAA ATPQRSQSPL RGGMTEAAQT
160 170 180 190 200
DKQCGQMPAT ATTAAAQPPT AHGPERSQSP AASDCSSSSS SASLPSSGRS
210 220 230 240 250
SLGSHQLPRG YIPIPVIHEQ NITRPAAQPS FHQAQKTHYP AQQGEYQPQQ
260 270 280 290 300
PVYHKIQGDD WEPRPLRAAS PFRSPVRGAS SREGSPARSG TPVHCPSPIR
310 320 330 340 350
VHTVVDRPQP MTHREPPPVT QPENKPESKP GPAGPDLPPG HIPIQVIRRE
360 370 380 390 400
ADSKPVSQKS PPPAEKVEVK VSSAPIPCPS PSPAPSAVPS PPKNVAAEQK
410 420 430 440 450
AAPSPAPAEP AAPKSGEAET PPKHPGVLKV EAILEKVQGL EQAVDSFEGK
460 470 480 490 500
KTDKKYLMIE EYLTKELLAL DSVDPEGRAD VRQARRDGVR KVQTILEKLE
510 520 530 540 550
QKAIDVPGQV QVYELQPSNL EAEQPLQEIM GAVVADKDKK GPENKDPQTE
560 570
SQQLEAKAAT PPNPSNPADS AGNLVAP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 74 | D → N in AAF26840 (PubMed:10597216).Curated | 1 | |
Sequence conflicti | 527 | Q → P in AAF26840 (PubMed:10597216).Curated | 1 | |
Sequence conflicti | 539 | K → E in BAA95066 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF130471 mRNA Translation: AAF26840.1 AB041583 mRNA Translation: BAA95066.1 AK007414 mRNA Translation: BAB25024.1 AK016510 mRNA Translation: BAB30278.1 AK036675 mRNA Translation: BAC29531.1 CH466531 Genomic DNA Translation: EDL17651.1 |
CCDSi | CCDS21898.1 |
RefSeqi | NP_038891.4, NM_013863.5 |
Genome annotation databases
Ensembli | ENSMUST00000033136; ENSMUSP00000033136; ENSMUSG00000030847 |
GeneIDi | 29810 |
KEGGi | mmu:29810 |
UCSCi | uc009jzb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF130471 mRNA Translation: AAF26840.1 AB041583 mRNA Translation: BAA95066.1 AK007414 mRNA Translation: BAB25024.1 AK016510 mRNA Translation: BAB30278.1 AK036675 mRNA Translation: BAC29531.1 CH466531 Genomic DNA Translation: EDL17651.1 |
CCDSi | CCDS21898.1 |
RefSeqi | NP_038891.4, NM_013863.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UK5 | NMR | - | A | 406-503 | [»] | |
SMRi | Q9JLV1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 205891, 70 interactors |
IntActi | Q9JLV1, 6 interactors |
MINTi | Q9JLV1 |
STRINGi | 10090.ENSMUSP00000033136 |
PTM databases
iPTMneti | Q9JLV1 |
PhosphoSitePlusi | Q9JLV1 |
SwissPalmi | Q9JLV1 |
Proteomic databases
jPOSTi | Q9JLV1 |
MaxQBi | Q9JLV1 |
PaxDbi | Q9JLV1 |
PRIDEi | Q9JLV1 |
Protocols and materials databases
Antibodypediai | 18850, 429 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000033136; ENSMUSP00000033136; ENSMUSG00000030847 |
GeneIDi | 29810 |
KEGGi | mmu:29810 |
UCSCi | uc009jzb.2, mouse |
Organism-specific databases
CTDi | 9531 |
MGIi | MGI:1352493, Bag3 |
Phylogenomic databases
eggNOGi | KOG0940, Eukaryota KOG4361, Eukaryota |
GeneTreei | ENSGT00940000159204 |
HOGENOMi | CLU_034378_0_0_1 |
InParanoidi | Q9JLV1 |
OMAi | THSPMMQ |
OrthoDBi | 715914at2759 |
TreeFami | TF102013 |
Enzyme and pathway databases
Reactomei | R-MMU-3371453, Regulation of HSF1-mediated heat shock response |
Miscellaneous databases
BioGRID-ORCSi | 29810, 0 hits in 17 CRISPR screens |
ChiTaRSi | Bag3, mouse |
EvolutionaryTracei | Q9JLV1 |
PROi | PR:Q9JLV1 |
RNActi | Q9JLV1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030847, Expressed in cardiac muscle of left ventricle and 262 other tissues |
Genevisiblei | Q9JLV1, MM |
Family and domain databases
CDDi | cd00201, WW, 1 hit |
Gene3Di | 1.20.58.120, 1 hit |
InterProi | View protein in InterPro IPR039773, BAG_chaperone_regulator IPR036533, BAG_dom_sf IPR003103, BAG_domain IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR12329, PTHR12329, 1 hit |
Pfami | View protein in Pfam PF02179, BAG, 1 hit PF00397, WW, 1 hit |
SMARTi | View protein in SMART SM00264, BAG, 1 hit SM00456, WW, 1 hit |
SUPFAMi | SSF51045, SSF51045, 1 hit SSF63491, SSF63491, 1 hit |
PROSITEi | View protein in PROSITE PS51035, BAG, 1 hit PS01159, WW_DOMAIN_1, 1 hit PS50020, WW_DOMAIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAG3_MOUSE | |
Accessioni | Q9JLV1Primary (citable) accession number: Q9JLV1 Secondary accession number(s): Q9CQL3, Q9JJC7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references