Litaf

Functionⁱ

Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. Plays a role in targeting endocytosed EGFR and ERGG3 for lysosomal degradation, and thereby helps downregulate downstream signaling cascades (PubMed:23166352). Helps recruit the ESCRT complex components TSG101, HGS and STAM to cytoplasmic membranes. Probably plays a role in regulating protein degradation via its interaction with NEDD4 (By similarity). May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role with STAT6 in the nucleus in regulating the expression of various cytokines (PubMed:15793005, PubMed:21980379). May regulate the expression of numerous cytokines, such as TNF, CCL2, CCL5, CXCL1, IL1A and IL10 (PubMed:12355436, PubMed:15025820, PubMed:16954198, PubMed:21980379, PubMed:22160695).By similarity6 Publications

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	96	ZincBy similarity	1
Metal bindingⁱ	99	ZincBy similarity	1
Metal bindingⁱ	148	ZincBy similarity	1
Metal bindingⁱ	151	ZincBy similarity	1

GO - Molecular functionⁱ

RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
WW domain binding Source: UniProtKB
zinc ion binding Source: UniProtKB

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

aging Source: Ensembl
cellular response to lipopolysaccharide
Source: MGI
Inferred from mutant phenotypeⁱ
- 16954198
negative regulation of NIK/NF-kappaB signaling
Source: MGI
Inferred from mutant phenotypeⁱ
- 12914755
positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
positive regulation of transcription by RNA polymerase II Source: MGI
regulation of cytokine production
Source: MGI
Inferred from mutant phenotypeⁱ
- 16954198
response to lipopolysaccharide
Source: MGI
Inferred from mutant phenotypeⁱ
- 22160695

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Transcription, Transcription regulation
Ligand	Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog Short name: LPS-induced TNF-alpha factor homolog Alternative name(s): Estrogen-enhanced transcript protein1 Publication Short name: mEET1 Publication LITAF-like protein NEDD4 WW domain-binding protein 3
Gene namesⁱ	Name:Litaf Synonyms:N4wbp3, Tbx1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 16

Organism-specific databases

MGIⁱ	MGI:1929512 Litaf

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasm, Endosome, Golgi apparatus, Lysosome, Membrane, Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

No visible phenotype. Mice are born at the expected Mendelian rate and are fertile. Mutant mice display altered responses to nerve crush injury, with higher numbers of macrophages in injured nerves five days after nerve crush injury, but at later time points macrophage numbers in injured nerves are normal. Bone marrow-derived macrophages from mutant mice display increased migration in response to CCL3, but not in the absence of CCL3 (PubMed:22729949). Mutant mice show dramatically increased survival in response to a dose of lipopolysaccharide (LPS) that causes rapid death of 40% of wild-type mice (PubMed:22160695).2 Publications

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	23	Y → A: Abolishes interaction with NEDD4. 1 Publication		1
Mutagenesisⁱ	61	Y → A: No effect on interaction with NEDD4. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000084441	1 – 161	Lipopolysaccharide-induced tumor necrosis factor-alpha factor homologAdd BLAST		161

Post-translational modificationⁱ

Phosphorylated on tyrosine residues in response to EGF.By similarity

Proteomic databases

PaxDbⁱ	Q9JLJ0
PRIDEⁱ	Q9JLJ0

PTM databases

iPTMnetⁱ	Q9JLJ0
PhosphoSitePlusⁱ	Q9JLJ0
SwissPalmⁱ	Q9JLJ0

Expressionⁱ

Tissue specificityⁱ

Detected in brain, heart, lung, liver, spleen and bone marrow (PubMed:22160695). Detected in myelinating Schwann cells in sciatic nerve and in bone marrow-derived macrophages (at protein level) (PubMed:22729949). Widely expressed. Highly expressed in liver.4 Publications

Developmental stageⁱ

Expression in sciatic nerve is low in neonates, culminates seven days after birth and decreases rapidly thereafter (at protein level) (PubMed:22729949). Strong expression is detected at E.7 and drops at E11.2 Publications

Inductionⁱ

Up-regulated in macrophages exposed to lipopolysaccharide (LPS) (at protein level) (PubMed:15793005). By estrogen and lipopolysaccharides (LPS).3 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000022500 Expressed in 300 organ(s), highest expression level in blood
CleanExⁱ	MM_LITAF MM_TBX1
ExpressionAtlasⁱ	Q9JLJ0 baseline and differential
Genevisibleⁱ	Q9JLJ0 MM

Interactionⁱ

Subunit structureⁱ

Monomer. Interacts with NEDD4 (PubMed:11042109). Interacts (via PSAP motif) with TSG101, a component of the ESCRT-I complex (endosomal sorting complex required for transport I). Interacts with WWOX. Interacts with STAM, a component of the ESCRT-0 complex; the interaction is direct. Identified in a complex with STAM and HGS; within this complex, interacts directly with STAM, but not with HGS. Interacts with STAT6 (PubMed:15793005).By similarity2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Nedd4	P46935	5	EBI-643664,EBI-773516

With

Entry

#Exp.

IntAct

Notes

Nedd4

P46935

5

EBI-643664,EBI-773516

GO - Molecular functionⁱ

WW domain binding Source: UniProtKB

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	208144, 2 interactors
IntActⁱ	Q9JLJ0, 2 interactors
STRINGⁱ	10090.ENSMUSP00000023143

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	76 – 160	LITAFPROSITE-ProRule annotationAdd BLAST		85

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	111 – 134	Membrane-binding amphipathic helixCuratedAdd BLAST		24

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	20 – 23	PPxY motif		4

Domainⁱ

The PPxY motif mediates interaction with WWOX and NEDD4.By similarity

The LITAF domain is stabilized by a bound zinc ion. The LITAF domain contains an amphiphatic helix that mediates interaction with lipid membranes. It interacts specifically with phosphatidylethanolamine lipid headgroups, but not with phosphoglycerol, phosphocholine, phosphoserine or inositolhexakisphosphate.By similarity

Sequence similaritiesⁱ

Belongs to the CDIP1/LITAF family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG410IVVU Eukaryota ENOG41122PJ LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00540000071542
HOGENOMⁱ	HOG000039585
HOVERGENⁱ	HBG006272
InParanoidⁱ	Q9JLJ0
KEGG Orthology (KO) More...KOⁱ	K19363
OMAⁱ	HCHAEID
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0XNM
PhylomeDBⁱ	Q9JLJ0
TreeFamⁱ	TF313294

Family and domain databases

InterProⁱ	View protein in InterPro IPR006629 LITAF IPR037519 LITAF_fam
PANTHERⁱ	PTHR23292 PTHR23292, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10601 zf-LITAF-like, 1 hit
SMARTⁱ	View protein in SMART SM00714 LITAF, 1 hit
PROSITEⁱ	View protein in PROSITE PS51837 LITAF, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q9JLJ0-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSAPGPYQAA AGPSVVPTAP PTYEETVGVN SYYPTPPAPM PGPATGLITG 
        60         70         80         90        100
PDGKGMNPPS YYTQPVPVPN ANAIAVQTVY VQQPVSFYDR PVQMCCPSCS 
       110        120        130        140        150
KMIVTQLSYN AGALTWLSCG SLCLLGCVAG CCFIPFCVDA LQDVDHYCPN 
       160 
CKALLGTYKR L

Length:161

Mass (Da):16,946

Last modified:October 1, 2000 - v1

Checksum:ⁱ7397F79C5AD0CD79

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	E0CYR5	E0CYR5_MOUSE		Lipopolysaccharide-induced tumor ne... Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog	Litaf	97	Annotation score:

Sequence cautionⁱ

The sequence AAG44246 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF171100 mRNA Translation: AAF27312.1 AF230522 mRNA Translation: AAO49168.1 AK018578 mRNA Translation: BAB31289.1 AK076162 mRNA Translation: BAC36228.1 AK151053 mRNA Translation: BAE30070.1 AK159533 mRNA Translation: BAE35161.1 BC018559 mRNA Translation: AAH18559.1 AF220207 mRNA Translation: AAG44246.1 Different initiation.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS27958.1
NCBI Reference Sequences More...RefSeqⁱ	NP_064364.1, NM_019980.2 XP_006522490.1, XM_006522427.1
UniGeneⁱ	Mm.294753

Genome annotation databases

Ensemblⁱ	ENSMUST00000023143; ENSMUSP00000023143; ENSMUSG00000022500 ENSMUST00000117360; ENSMUSP00000112667; ENSMUSG00000022500
GeneIDⁱ	56722
KEGGⁱ	mmu:56722
UCSC genome browser More...UCSCⁱ	uc007yen.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9JLJ0	Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog (Fragment)		MOUSE		97	UniRef100_Q9JLJ0

Protein	Similar proteins		Species	Score	Length	Source
Q9JLJ0	Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog		RAT		161	UniRef90_Q9JLJ0
Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog (Fragment)		MOUSE		97
lipopolysaccharide-induced tumor necrosis factor-alpha factor isoform X1		Florida manatee		194
Lipopolysaccharide induced TNF factor		HORSE		161
Lipopolysaccharide induced TNF factor		PIG		161
+30

Protein	Similar proteins		Species	Score	Length	Source
Q9JLJ0	Lipopolysaccharide-induced tumor necrosis factor-alpha factor		HUMAN		161	UniRef50_Q99732
Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog		RAT		161
LITAF isoform 14		PONAB		68
LITAF isoform 14		PANTR		68
Uncharacterized protein		RHIBE		161
+108

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF171100 mRNA Translation: AAF27312.1 AF230522 mRNA Translation: AAO49168.1 AK018578 mRNA Translation: BAB31289.1 AK076162 mRNA Translation: BAC36228.1 AK151053 mRNA Translation: BAE30070.1 AK159533 mRNA Translation: BAE35161.1 BC018559 mRNA Translation: AAH18559.1 AF220207 mRNA Translation: AAG44246.1 Different initiation.
CCDSⁱ	CCDS27958.1
RefSeqⁱ	NP_064364.1, NM_019980.2 XP_006522490.1, XM_006522427.1
UniGeneⁱ	Mm.294753

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	208144, 2 interactors
IntActⁱ	Q9JLJ0, 2 interactors
STRINGⁱ	10090.ENSMUSP00000023143

PTM databases

iPTMnetⁱ	Q9JLJ0
PhosphoSitePlusⁱ	Q9JLJ0
SwissPalmⁱ	Q9JLJ0

Proteomic databases

PaxDbⁱ	Q9JLJ0
PRIDEⁱ	Q9JLJ0

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000023143; ENSMUSP00000023143; ENSMUSG00000022500 ENSMUST00000117360; ENSMUSP00000112667; ENSMUSG00000022500
GeneIDⁱ	56722
KEGGⁱ	mmu:56722
UCSCⁱ	uc007yen.2 mouse

Organism-specific databases

CTDⁱ	9516
MGIⁱ	MGI:1929512 Litaf

Phylogenomic databases

eggNOGⁱ	ENOG410IVVU Eukaryota ENOG41122PJ LUCA
GeneTreeⁱ	ENSGT00540000071542
HOGENOMⁱ	HOG000039585
HOVERGENⁱ	HBG006272
InParanoidⁱ	Q9JLJ0
KOⁱ	K19363
OMAⁱ	HCHAEID
OrthoDBⁱ	EOG091G0XNM
PhylomeDBⁱ	Q9JLJ0
TreeFamⁱ	TF313294

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9JLJ0
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000022500 Expressed in 300 organ(s), highest expression level in blood
CleanExⁱ	MM_LITAF MM_TBX1
ExpressionAtlasⁱ	Q9JLJ0 baseline and differential
Genevisibleⁱ	Q9JLJ0 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR006629 LITAF IPR037519 LITAF_fam
PANTHERⁱ	PTHR23292 PTHR23292, 1 hit
Pfamⁱ	View protein in Pfam PF10601 zf-LITAF-like, 1 hit
SMARTⁱ	View protein in SMART SM00714 LITAF, 1 hit
PROSITEⁱ	View protein in PROSITE PS51837 LITAF, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	LITAF_MOUSE
Accessionⁱ	Q9JLJ0Primary (citable) accession number: Q9JLJ0 Secondary accession number(s): Q9EQI0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
	Last sequence update:	October 1, 2000
	Last modified:	November 7, 2018
	This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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UniProtKB - Q9JLJ0 (LITAF_MOUSE)

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Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Endosome

Plasma membrane

Lysosome

Golgi apparatus

Other locations

Cytosol

Endosome

Golgi apparatus

Lysosome

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ