UniProtKB - Q9JLJ0 (LITAF_MOUSE)
Protein
Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
Gene
Litaf
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. Plays a role in targeting endocytosed EGFR and ERGG3 for lysosomal degradation, and thereby helps downregulate downstream signaling cascades (PubMed:23166352). Helps recruit the ESCRT complex components TSG101, HGS and STAM to cytoplasmic membranes. Probably plays a role in regulating protein degradation via its interaction with NEDD4 (By similarity). May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role with STAT6 in the nucleus in regulating the expression of various cytokines (PubMed:15793005, PubMed:21980379). May regulate the expression of numerous cytokines, such as TNF, CCL2, CCL5, CXCL1, IL1A and IL10 (PubMed:12355436, PubMed:15025820, PubMed:16954198, PubMed:21980379, PubMed:22160695).By similarity6 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 96 | ZincBy similarity | 1 | |
Metal bindingi | 99 | ZincBy similarity | 1 | |
Metal bindingi | 148 | ZincBy similarity | 1 | |
Metal bindingi | 151 | ZincBy similarity | 1 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- WW domain binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- aging Source: Ensembl
- cellular response to lipopolysaccharide Source: MGI
- negative regulation of NIK/NF-kappaB signaling Source: MGI
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of cytokine production Source: MGI
- response to lipopolysaccharide Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Lipopolysaccharide-induced tumor necrosis factor-alpha factor homologShort name: LPS-induced TNF-alpha factor homolog Alternative name(s): Estrogen-enhanced transcript protein1 Publication Short name: mEET1 Publication LITAF-like protein NEDD4 WW domain-binding protein 3 |
Gene namesi | Name:Litaf Synonyms:N4wbp3, Tbx1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1929512, Litaf |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Golgi apparatus
- Golgi apparatus membrane By similarity
Lysosome
- Lysosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Nucleus
- Nucleus By similarity
Endosome
- Early endosome membrane By similarity
- Late endosome membrane By similarity
- Endosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Other locations
- Cytoplasm By similarity
Note: Associated with membranes of lysosomes, early and late endosomes. Can translocate from the cytoplasm into the nucleus (By similarity). Detected at Schmidt-Lanterman incisures and in nodal regions of myelinating Schwann cells (PubMed:22729949).By similarity1 Publication
Cytosol
- cytosol Source: MGI
Endosome
- cytoplasmic side of early endosome membrane Source: UniProtKB
- cytoplasmic side of late endosome membrane Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Lysosome
- cytoplasmic side of lysosomal membrane Source: UniProtKB
- lysosomal membrane Source: MGI
Nucleus
- nucleoplasm Source: MGI
Plasma Membrane
- cytoplasmic side of plasma membrane Source: UniProtKB
- plasma membrane Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Endosome, Golgi apparatus, Lysosome, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
No visible phenotype. Mice are born at the expected Mendelian rate and are fertile. Mutant mice display altered responses to nerve crush injury, with higher numbers of macrophages in injured nerves five days after nerve crush injury, but at later time points macrophage numbers in injured nerves are normal. Bone marrow-derived macrophages from mutant mice display increased migration in response to CCL3, but not in the absence of CCL3 (PubMed:22729949). Mutant mice show dramatically increased survival in response to a dose of lipopolysaccharide (LPS) that causes rapid death of 40% of wild-type mice (PubMed:22160695).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 23 | Y → A: Abolishes interaction with NEDD4. 1 Publication | 1 | |
Mutagenesisi | 61 | Y → A: No effect on interaction with NEDD4. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084441 | 1 – 161 | Lipopolysaccharide-induced tumor necrosis factor-alpha factor homologAdd BLAST | 161 |
Post-translational modificationi
Phosphorylated on tyrosine residues in response to EGF.By similarity
Proteomic databases
PaxDbi | Q9JLJ0 |
PRIDEi | Q9JLJ0 |
PTM databases
iPTMneti | Q9JLJ0 |
PhosphoSitePlusi | Q9JLJ0 |
SwissPalmi | Q9JLJ0 |
Expressioni
Tissue specificityi
Detected in brain, heart, lung, liver, spleen and bone marrow (PubMed:22160695). Detected in myelinating Schwann cells in sciatic nerve and in bone marrow-derived macrophages (at protein level) (PubMed:22729949). Widely expressed. Highly expressed in liver.4 Publications
Developmental stagei
Expression in sciatic nerve is low in neonates, culminates seven days after birth and decreases rapidly thereafter (at protein level) (PubMed:22729949). Strong expression is detected at E.7 and drops at 11 dpc.2 Publications
Inductioni
Up-regulated in macrophages exposed to lipopolysaccharide (LPS) (at protein level) (PubMed:15793005). By estrogen and lipopolysaccharides (LPS).3 Publications
Gene expression databases
Bgeei | ENSMUSG00000022500, Expressed in blood and 317 other tissues |
ExpressionAtlasi | Q9JLJ0, baseline and differential |
Genevisiblei | Q9JLJ0, MM |
Interactioni
Subunit structurei
Monomer.
Interacts with NEDD4 (PubMed:11042109).
Interacts (via PSAP motif) with TSG101, a component of the ESCRT-I complex (endosomal sorting complex required for transport I).
Interacts with WWOX.
Interacts with STAM, a component of the ESCRT-0 complex; the interaction is direct.
Identified in a complex with STAM and HGS; within this complex, interacts directly with STAM, but not with HGS.
Interacts with STAT6 (PubMed:15793005).
By similarity2 PublicationsBinary interactionsi
Q9JLJ0
With | #Exp. | IntAct |
---|---|---|
Nedd4 [P46935] | 5 | EBI-643664,EBI-773516 |
GO - Molecular functioni
- WW domain binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 208144, 2 interactors |
IntActi | Q9JLJ0, 2 interactors |
STRINGi | 10090.ENSMUSP00000023143 |
Miscellaneous databases
RNActi | Q9JLJ0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 76 – 160 | LITAFPROSITE-ProRule annotationAdd BLAST | 85 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 111 – 134 | Membrane-binding amphipathic helixCuratedAdd BLAST | 24 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 20 – 23 | PPxY motif | 4 |
Domaini
The PPxY motif mediates interaction with WWOX and NEDD4.By similarity
The LITAF domain is stabilized by a bound zinc ion. The LITAF domain contains an amphiphatic helix that mediates interaction with lipid membranes. It interacts specifically with phosphatidylethanolamine lipid headgroups, but not with phosphoglycerol, phosphocholine, phosphoserine or inositolhexakisphosphate.By similarity
Sequence similaritiesi
Belongs to the CDIP1/LITAF family.Curated
Phylogenomic databases
eggNOGi | ENOG502S2GM, Eukaryota |
GeneTreei | ENSGT00940000155366 |
HOGENOMi | CLU_095549_3_0_1 |
InParanoidi | Q9JLJ0 |
OMAi | FCIDALQ |
OrthoDBi | 354219at2759 |
PhylomeDBi | Q9JLJ0 |
TreeFami | TF313294 |
Family and domain databases
InterProi | View protein in InterPro IPR006629, LITAF IPR037519, LITAF_fam |
PANTHERi | PTHR23292, PTHR23292, 1 hit |
Pfami | View protein in Pfam PF10601, zf-LITAF-like, 1 hit |
SMARTi | View protein in SMART SM00714, LITAF, 1 hit |
PROSITEi | View protein in PROSITE PS51837, LITAF, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9JLJ0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAPGPYQAA AGPSVVPTAP PTYEETVGVN SYYPTPPAPM PGPATGLITG
60 70 80 90 100
PDGKGMNPPS YYTQPVPVPN ANAIAVQTVY VQQPVSFYDR PVQMCCPSCS
110 120 130 140 150
KMIVTQLSYN AGALTWLSCG SLCLLGCVAG CCFIPFCVDA LQDVDHYCPN
160
CKALLGTYKR L
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE0CYR5 | E0CYR5_MOUSE | Lipopolysaccharide-induced tumor ne... | Litaf | 97 | Annotation score: |
Sequence cautioni
The sequence AAG44246 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF171100 mRNA Translation: AAF27312.1 AF230522 mRNA Translation: AAO49168.1 AK018578 mRNA Translation: BAB31289.1 AK076162 mRNA Translation: BAC36228.1 AK151053 mRNA Translation: BAE30070.1 AK159533 mRNA Translation: BAE35161.1 BC018559 mRNA Translation: AAH18559.1 AF220207 mRNA Translation: AAG44246.1 Different initiation. |
CCDSi | CCDS27958.1 |
RefSeqi | NP_064364.1, NM_019980.2 XP_006522490.1, XM_006522427.1 |
Genome annotation databases
Ensembli | ENSMUST00000023143; ENSMUSP00000023143; ENSMUSG00000022500 ENSMUST00000117360; ENSMUSP00000112667; ENSMUSG00000022500 |
GeneIDi | 56722 |
KEGGi | mmu:56722 |
UCSCi | uc007yen.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF171100 mRNA Translation: AAF27312.1 AF230522 mRNA Translation: AAO49168.1 AK018578 mRNA Translation: BAB31289.1 AK076162 mRNA Translation: BAC36228.1 AK151053 mRNA Translation: BAE30070.1 AK159533 mRNA Translation: BAE35161.1 BC018559 mRNA Translation: AAH18559.1 AF220207 mRNA Translation: AAG44246.1 Different initiation. |
CCDSi | CCDS27958.1 |
RefSeqi | NP_064364.1, NM_019980.2 XP_006522490.1, XM_006522427.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 208144, 2 interactors |
IntActi | Q9JLJ0, 2 interactors |
STRINGi | 10090.ENSMUSP00000023143 |
PTM databases
iPTMneti | Q9JLJ0 |
PhosphoSitePlusi | Q9JLJ0 |
SwissPalmi | Q9JLJ0 |
Proteomic databases
PaxDbi | Q9JLJ0 |
PRIDEi | Q9JLJ0 |
Protocols and materials databases
Antibodypediai | 1839, 286 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000023143; ENSMUSP00000023143; ENSMUSG00000022500 ENSMUST00000117360; ENSMUSP00000112667; ENSMUSG00000022500 |
GeneIDi | 56722 |
KEGGi | mmu:56722 |
UCSCi | uc007yen.2, mouse |
Organism-specific databases
CTDi | 9516 |
MGIi | MGI:1929512, Litaf |
Phylogenomic databases
eggNOGi | ENOG502S2GM, Eukaryota |
GeneTreei | ENSGT00940000155366 |
HOGENOMi | CLU_095549_3_0_1 |
InParanoidi | Q9JLJ0 |
OMAi | FCIDALQ |
OrthoDBi | 354219at2759 |
PhylomeDBi | Q9JLJ0 |
TreeFami | TF313294 |
Miscellaneous databases
BioGRID-ORCSi | 56722, 1 hit in 17 CRISPR screens |
ChiTaRSi | Litaf, mouse |
PROi | PR:Q9JLJ0 |
RNActi | Q9JLJ0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022500, Expressed in blood and 317 other tissues |
ExpressionAtlasi | Q9JLJ0, baseline and differential |
Genevisiblei | Q9JLJ0, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006629, LITAF IPR037519, LITAF_fam |
PANTHERi | PTHR23292, PTHR23292, 1 hit |
Pfami | View protein in Pfam PF10601, zf-LITAF-like, 1 hit |
SMARTi | View protein in SMART SM00714, LITAF, 1 hit |
PROSITEi | View protein in PROSITE PS51837, LITAF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LITAF_MOUSE | |
Accessioni | Q9JLJ0Primary (citable) accession number: Q9JLJ0 Secondary accession number(s): Q9EQI0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families