UniProtKB - Q9JLF7 (TLR5_MOUSE)
Protein
Toll-like receptor 5
Gene
Tlr5
Organism
Mus musculus (Mouse)
Status
Functioni
Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life.By similarity1 Publication
GO - Molecular functioni
- interleukin-1 receptor binding Source: MGI
- signaling receptor activity Source: GO_Central
- transmembrane signaling receptor activity Source: InterPro
GO - Biological processi
- defense response to bacterium Source: InterPro
- inflammatory response Source: GO_Central
- innate immune response Source: UniProtKB-KW
- MyD88-dependent toll-like receptor signaling pathway Source: InterPro
- positive regulation of interleukin-8 production Source: MGI
- positive regulation of nitric oxide biosynthetic process Source: MGI
- positive regulation of toll-like receptor signaling pathway Source: MGI
- toll-like receptor 5 signaling pathway Source: InterPro
- toll-like receptor signaling pathway Source: GO_Central
Keywordsi
Molecular function | Receptor |
Biological process | Immunity, Inflammatory response, Innate immunity |
Names & Taxonomyi
Protein namesi | Recommended name: Toll-like receptor 5 |
Gene namesi | Name:Tlr5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1858171, Tlr5 |
Subcellular locationi
Other locations
- Membrane By similarity; Single-pass type I membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Other locations
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 27 – 641 | ExtracellularSequence analysisAdd BLAST | 615 | |
Transmembranei | 642 – 662 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 663 – 859 | CytoplasmicSequence analysisAdd BLAST | 197 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
Deficient mice exhibit a markedly altered enteric microbiota composition, with increased levels of fecal flagellin.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000034730 | 27 – 859 | Toll-like receptor 5Add BLAST | 833 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 37 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 46 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 84 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 246 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 343 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 438 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 584 ↔ 611 | By similarity | ||
Disulfide bondi | 586 ↔ 630 | By similarity | ||
Glycosylationi | 596 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 599 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 624 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 799 | PhosphotyrosineBy similarity | 1 |
Post-translational modificationi
Phosphorylated at Tyr-799 upon flagellin binding; required for signaling.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q9JLF7 |
PRIDEi | Q9JLF7 |
PTM databases
GlyGeni | Q9JLF7, 9 sites |
iPTMneti | Q9JLF7 |
PhosphoSitePlusi | Q9JLF7 |
Expressioni
Tissue specificityi
Highly expressed in liver (PubMed:30089902). Detected in lung and at very low levels in most other tissues.1 Publication
Interactioni
Subunit structurei
Homodimer.
Interacts with MYD88 (via TIR domain).
Interacts with TICAM1 (via TIR domain).
Interacts with UNC93B1; this interaction is essential for proper TLR5 localization to the plasma membrane.
By similarityBinary interactionsi
Hide detailsQ9JLF7
With | #Exp. | IntAct |
---|---|---|
flag [Q56086] from Salmonella typhi. | 2 | EBI-6548397,EBI-6548623 |
fliC [A0A0H3NMJ6] from Salmonella typhimurium (strain SL1344). | 2 | EBI-6548397,EBI-6548383 |
GO - Molecular functioni
- interleukin-1 receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 207467, 2 interactors |
IntActi | Q9JLF7, 6 interactors |
STRINGi | 10090.ENSMUSP00000106625 |
Miscellaneous databases
RNActi | Q9JLF7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 45 – 69 | LRR 1Add BLAST | 25 | |
Repeati | 72 – 94 | LRR 2Add BLAST | 23 | |
Repeati | 96 – 118 | LRR 3Add BLAST | 23 | |
Repeati | 121 – 144 | LRR 4Add BLAST | 24 | |
Repeati | 147 – 167 | LRR 5Add BLAST | 21 | |
Repeati | 172 – 193 | LRR 6Add BLAST | 22 | |
Repeati | 198 – 212 | LRR 7Add BLAST | 15 | |
Repeati | 215 – 230 | LRR 8Add BLAST | 16 | |
Repeati | 235 – 236 | LRR 9 | 2 | |
Repeati | 261 – 285 | LRR 11Add BLAST | 25 | |
Repeati | 290 – 302 | LRR 12Add BLAST | 13 | |
Repeati | 314 – 335 | LRR 13Add BLAST | 22 | |
Repeati | 338 – 356 | LRR 14Add BLAST | 19 | |
Repeati | 386 – 402 | LRR 16Add BLAST | 17 | |
Repeati | 413 – 432 | LRR 17Add BLAST | 20 | |
Repeati | 450 – 471 | LRR 18Add BLAST | 22 | |
Repeati | 475 – 496 | LRR 19Add BLAST | 22 | |
Repeati | 504 – 525 | LRR 20Add BLAST | 22 | |
Repeati | 528 – 547 | LRR 21Add BLAST | 20 | |
Repeati | 550 – 568 | LRR 22Add BLAST | 19 | |
Domaini | 580 – 632 | LRRCTAdd BLAST | 53 | |
Domaini | 692 – 837 | TIRPROSITE-ProRule annotationAdd BLAST | 146 |
Sequence similaritiesi
Belongs to the Toll-like receptor family.Curated
Keywords - Domaini
Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4641, Eukaryota |
InParanoidi | Q9JLF7 |
PhylomeDBi | Q9JLF7 |
Family and domain databases
Gene3Di | 3.40.50.10140, 1 hit 3.80.10.10, 4 hits |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR000157, TIR_dom IPR027176, TLR5 IPR017241, Toll-like_receptor IPR035897, Toll_tir_struct_dom_sf |
PANTHERi | PTHR24365, PTHR24365, 1 hit PTHR24365:SF525, PTHR24365:SF525, 1 hit |
Pfami | View protein in Pfam PF13855, LRR_8, 3 hits PF01582, TIR, 1 hit |
PIRSFi | PIRSF037595, Toll-like_receptor, 1 hit |
SMARTi | View protein in SMART SM00369, LRR_TYP, 9 hits SM00082, LRRCT, 1 hit SM00255, TIR, 1 hit |
SUPFAMi | SSF52200, SSF52200, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 14 hits PS50104, TIR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9JLF7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MACQLDLLIG VIFMASPVLV ISPCSSDGRI AFFRGCNLTQ IPWILNTTTE
60 70 80 90 100
RLLLSFNYIS MVVATSFPLL ERLQLLELGT QYANLTIGPG AFRNLPNLRI
110 120 130 140 150
LDLGQSQIEV LNRDAFQGLP HLLELRLFSC GLSSAVLSDG YFRNLYSLAR
160 170 180 190 200
LDLSGNQIHS LRLHSSFREL NSLSDVNFAF NQIFTICEDE LEPLQGKTLS
210 220 230 240 250
FFGLKLTKLF SRVSVGWETC RNPFRGVRLE TLDLSENGWT VDITRNFSNI
260 270 280 290 300
IQGSQISSLI LKHHIMGPGF GFQNIRDPDQ STFASLARSS VLQLDLSHGF
310 320 330 340 350
IFSLNPRLFG TLKDLKMLNL AFNKINKIGE NAFYGLDSLQ VLNLSYNLLG
360 370 380 390 400
ELYNSNFYGL PRVAYVDLQR NHIGIIQDQT FRLLKTLQTL DLRDNALKAI
410 420 430 440 450
GFIPSIQMVL LGGNKLVHLP HIHFTANFLE LSENRLENLS DLYFLLRVPQ
460 470 480 490 500
LQFLILNQNR LSSCKAAHTP SENPSLEQLF LTENMLQLAW ETGLCWDVFQ
510 520 530 540 550
GLSRLQILYL SNNYLNFLPP GIFNDLVALR MLSLSANKLT VLSPGSLPAN
560 570 580 590 600
LEILDISRNQ LLCPDPALFS SLRVLDITHN EFVCNCELST FISWLNQTNV
610 620 630 640 650
TLFGSPADVY CMYPNSLLGG SLYNISTEDC DEEEAMRSLK FSLFILCTVT
660 670 680 690 700
LTLFLVITLV VIKFRGICFL CYKTIQKLVF KDKVWSLEPG AYRYDAYFCF
710 720 730 740 750
SSKDFEWAQN ALLKHLDAHY SSRNRLRLCF EERDFIPGEN HISNIQAAVW
760 770 780 790 800
GSRKTVCLVS RHFLKDGWCL EAFRYAQSRS LSDLKSILIV VVVGSLSQYQ
810 820 830 840 850
LMRHETIRGF LQKQQYLRWP EDLQDVGWFL DKLSGCILKE EKGKKRSSSI
QLRTIATIS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QL10 | E9QL10_MOUSE | Toll-like receptor 5 | Tlr5 | 873 | Annotation score: | ||
Q05AH3 | Q05AH3_MOUSE | Tlr5 protein | Tlr5 | 859 | Annotation score: | ||
A0A0A6YVY4 | A0A0A6YVY4_MOUSE | Toll-like receptor 5 | Tlr5 | 853 | Annotation score: |
Polymorphismi
The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 226 | G → D in strain: MOLF/Ei. | 1 | |
Natural varianti | 399 | A → T in strain: MOLF/Ei. | 1 | |
Natural varianti | 470 | P → T in strain: MOLF/Ei. | 1 | |
Natural varianti | 482 | T → A in strain: MOLF/Ei. | 1 | |
Natural varianti | 609 | V → M in strain: MOLF/Ei. | 1 | |
Natural varianti | 619 | G → A in strain: MOLF/Ei. | 1 | |
Natural varianti | 835 | G → E in strain: MOLF/Ei. | 1 | |
Natural varianti | 843 | G → A in strain: MOLF/Ei. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF186107 mRNA Translation: AAF65625.1 |
CCDSi | CCDS35815.1 |
RefSeqi | NP_058624.2, NM_016928.3 |
Genome annotation databases
GeneIDi | 53791 |
KEGGi | mmu:53791 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF186107 mRNA Translation: AAF65625.1 |
CCDSi | CCDS35815.1 |
RefSeqi | NP_058624.2, NM_016928.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 207467, 2 interactors |
IntActi | Q9JLF7, 6 interactors |
STRINGi | 10090.ENSMUSP00000106625 |
PTM databases
GlyGeni | Q9JLF7, 9 sites |
iPTMneti | Q9JLF7 |
PhosphoSitePlusi | Q9JLF7 |
Proteomic databases
PaxDbi | Q9JLF7 |
PRIDEi | Q9JLF7 |
Genome annotation databases
GeneIDi | 53791 |
KEGGi | mmu:53791 |
Organism-specific databases
CTDi | 7100 |
MGIi | MGI:1858171, Tlr5 |
Phylogenomic databases
eggNOGi | KOG4641, Eukaryota |
InParanoidi | Q9JLF7 |
PhylomeDBi | Q9JLF7 |
Miscellaneous databases
BioGRID-ORCSi | 53791, 0 hits in 12 CRISPR screens |
PROi | PR:Q9JLF7 |
RNActi | Q9JLF7, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 3.40.50.10140, 1 hit 3.80.10.10, 4 hits |
InterProi | View protein in InterPro IPR000483, Cys-rich_flank_reg_C IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR000157, TIR_dom IPR027176, TLR5 IPR017241, Toll-like_receptor IPR035897, Toll_tir_struct_dom_sf |
PANTHERi | PTHR24365, PTHR24365, 1 hit PTHR24365:SF525, PTHR24365:SF525, 1 hit |
Pfami | View protein in Pfam PF13855, LRR_8, 3 hits PF01582, TIR, 1 hit |
PIRSFi | PIRSF037595, Toll-like_receptor, 1 hit |
SMARTi | View protein in SMART SM00369, LRR_TYP, 9 hits SM00082, LRRCT, 1 hit SM00255, TIR, 1 hit |
SUPFAMi | SSF52200, SSF52200, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 14 hits PS50104, TIR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TLR5_MOUSE | |
Accessioni | Q9JLF7Primary (citable) accession number: Q9JLF7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 31, 2002 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families