Tef

Functionⁱ

Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'-[TC][AG][AG]TTA[TC][AG]-3' (By similarity). Also activates the telokin promoter in smooth muscle-specific and calcium-dependent manner.By similarity

Miscellaneous

Mice deficient for all three PAR bZIP proteins (DBP, HLF and TEF) display a dramatically shortened life span and are highly susceptible to generalized spontaneous and audiogenic epilepsies (due for example to the noise of a vacuum cleaner) that are frequently lethal. The down-regulation of pyridoxal kinase (Pdxk) expression in these mice may participate in this seizure phenotype.

GO - Molecular functionⁱ

double-stranded DNA binding
Source: MGI
Inferred from direct assayⁱ
- 1916262
protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI
RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
RNA polymerase II regulatory region sequence-specific DNA binding Source: GO_Central
sequence-specific DNA binding Source: MGI
transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

multicellular organism development Source: GO_Central
positive regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 1916262
rhythmic process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Biological rhythms, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Thyrotroph embryonic factor
Gene namesⁱ	Name:Tef
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 15

Organism-specific databases

MGIⁱ	MGI:98663 Tef

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000076513	1 – 301	Thyrotroph embryonic factorAdd BLAST		301

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	30	PhosphoserineBy similarity		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q9JLC6
PRIDEⁱ	Q9JLC6

PTM databases

iPTMnetⁱ	Q9JLC6
PhosphoSitePlusⁱ	Q9JLC6

Expressionⁱ

Tissue specificityⁱ

Isoform Alpha and isoform Beta are expressed at high levels in lung, bladder, kidney, gut and brain.

Inductionⁱ

Accumulates according to a robust circadian rhythm in liver and kidney. In liver nuclei, the amplitude of daily oscillation has been estimated to be 9-fold. Expressed at nearly constant level in the brain.

Gene expression databases

Bgeeⁱ	ENSMUSG00000022389 Expressed in 288 organ(s), highest expression level in cerebellum
CleanExⁱ	MM_TEF
ExpressionAtlasⁱ	Q9JLC6 baseline and differential
Genevisibleⁱ	Q9JLC6 MM

Interactionⁱ

Subunit structureⁱ

Binds DNA as a homodimer or a heterodimer. Can form a heterodimer with DBP (By similarity).By similarity

GO - Molecular functionⁱ

protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	204106, 3 interactors
STRINGⁱ	10090.ENSMUSP00000023024

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9JLC6
SMRⁱ	Q9JLC6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	231 – 294	bZIPPROSITE-ProRule annotationAdd BLAST		64

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	233 – 253	Basic motifPROSITE-ProRule annotationAdd BLAST		21
Regionⁱ	254 – 261	Leucine-zipperPROSITE-ProRule annotation		8

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	164 – 215	Pro-rich (proline/acidic region (PAR))Add BLAST		52

Sequence similaritiesⁱ

Belongs to the bZIP family. PAR subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG3119 Eukaryota ENOG410YJ8G LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00440000039070
HOVERGENⁱ	HBG004983
InParanoidⁱ	Q9JLC6
KEGG Orthology (KO) More...KOⁱ	K09058
OMAⁱ	SKYETRY
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0EO0
PhylomeDBⁱ	Q9JLC6
TreeFamⁱ	TF315869

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR029832 TEF/VBP
PANTHERⁱ	PTHR11988:SF24 PTHR11988:SF24, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07716 bZIP_2, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative promoter usage. Align Add to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform Alpha (identifier: Q9JLC6-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDAGGGKKP PVEPQAGPGP GRAAGERGLS GSFPLVLKKL MENPPRETRL 
        60         70         80         90        100
DKEKGKEKLE EDESAAASTM AVSASLMPPI WDKTIPYDGE SFHLEYMDLD 
       110        120        130        140        150
EFLLENGIPA SPTHLAQNLL LPVAELEGKE SASSSTASPP SSSTAIFQPS 
       160        170        180        190        200
ETVSSTESSL EKERETPSPI DPSCVEVDVN FNPDPADLVL SSVPGGELFN 
       210        220        230        240        250
PRKHRFAEED LKPQPMIKKA KKVFVPDEQK DEKYWTRRKK NNVAAKRSRD 
       260        270        280        290        300
ARRLKENQIT IRAAFLEKEN TALRTEVAEL RKEVGKCKTI VSKYETKYGP 

L

Length:301

Mass (Da):33,145

Last modified:October 1, 2000 - v1

Checksum:ⁱF83FFFC6D7E091A4

GO

Isoform Beta (identifier: Q9JLC6-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-50: MSDAGGGKKP...MENPPRETRL → MSSCSQIGVAPAMDMPEVLKSLLEHSLPWSEKKA

« Hide

        10         20         30         40         50
MSSCSQIGVA PAMDMPEVLK SLLEHSLPWS EKKADKEKGK EKLEEDESAA 
        60         70         80         90        100
ASTMAVSASL MPPIWDKTIP YDGESFHLEY MDLDEFLLEN GIPASPTHLA 
       110        120        130        140        150
QNLLLPVAEL EGKESASSST ASPPSSSTAI FQPSETVSST ESSLEKERET 
       160        170        180        190        200
PSPIDPSCVE VDVNFNPDPA DLVLSSVPGG ELFNPRKHRF AEEDLKPQPM 
       210        220        230        240        250
IKKAKKVFVP DEQKDEKYWT RRKKNNVAAK RSRDARRLKE NQITIRAAFL 
       260        270        280 
EKENTALRTE VAELRKEVGK CKTIVSKYET KYGPL

Show »

Length:285

Mass (Da):31,707

Checksum:ⁱ9478FD36092F41EA

GO

Isoform 2 (identifier: Q9JLC6-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-50: MSDAGGGKKPPVEPQAGPGPGRAAGERGLSGSFPLVLKKLMENPPRETRL → MDMPEVLKSLLEHSLPWSEKKA

« Hide

        10         20         30         40         50
MDMPEVLKSL LEHSLPWSEK KADKEKGKEK LEEDESAAAS TMAVSASLMP 
        60         70         80         90        100
PIWDKTIPYD GESFHLEYMD LDEFLLENGI PASPTHLAQN LLLPVAELEG 
       110        120        130        140        150
KESASSSTAS PPSSSTAIFQ PSETVSSTES SLEKERETPS PIDPSCVEVD 
       160        170        180        190        200
VNFNPDPADL VLSSVPGGEL FNPRKHRFAE EDLKPQPMIK KAKKVFVPDE 
       210        220        230        240        250
QKDEKYWTRR KKNNVAAKRS RDARRLKENQ ITIRAAFLEK ENTALRTEVA 
       260        270 
ELRKEVGKCK TIVSKYETKY GPL

Show »

Length:273

Mass (Da):30,575

Checksum:ⁱ49626F12DE8BAE6E

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

E9Q331	E9Q331_MOUSE	Thyrotroph embryonic factor Thyrotroph embryonic factor	Tef	106	Annotation score:
E9Q3N8	E9Q3N8_MOUSE	Thyrotroph embryonic factor Thyrotroph embryonic factor	Tef	70	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	38	K → R in BAE26249 (PubMed:16141072).Curated	1
Sequence conflictⁱ	97	M → V in BAC35849 (PubMed:16141072).Curated	1
Sequence conflictⁱ	191	S → C in BAE28255 (PubMed:16141072).Curated	1
Sequence conflictⁱ	219	K → E in BAE26249 (PubMed:16141072).Curated	1
Sequence conflictⁱ	224	F → L in BAE26249 (PubMed:16141072).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_011246	1 – 50	MSDAG…RETRL → MDMPEVLKSLLEHSLPWSEK KA in isoform 2. 1 PublicationAdd BLAST		50
Alternative sequenceⁱVSP_011245	1 – 50	MSDAG…RETRL → MSSCSQIGVAPAMDMPEVLK SLLEHSLPWSEKKA in isoform Beta. 2 PublicationsAdd BLAST		50

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF194420 mRNA Translation: AAF70830.1 AY540631 mRNA Translation: AAS45599.1 AY540632 mRNA Translation: AAS45600.1 AK075601 mRNA Translation: BAC35849.1 AK141606 mRNA Translation: BAE24760.1 AK145125 mRNA Translation: BAE26249.1 AK147965 mRNA Translation: BAE28255.1 AK154472 mRNA Translation: BAE32609.1 BC017689 mRNA Translation: AAH17689.1 BC036982 mRNA Translation: AAH36982.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS27671.2 [Q9JLC6-2] CCDS27672.1 [Q9JLC6-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_059072.1, NM_017376.3 [Q9JLC6-1] NP_705617.2, NM_153484.3 [Q9JLC6-2]
UniGeneⁱ	Mm.270278

Genome annotation databases

Ensemblⁱ	ENSMUST00000023024; ENSMUSP00000023024; ENSMUSG00000022389 [Q9JLC6-1] ENSMUST00000109553; ENSMUSP00000105180; ENSMUSG00000022389 [Q9JLC6-2]
GeneIDⁱ	21685
KEGGⁱ	mmu:21685
UCSC genome browser More...UCSCⁱ	uc007wxj.2 mouse [Q9JLC6-2] uc007wxk.2 mouse [Q9JLC6-1]

Keywords - Coding sequence diversityⁱ

Alternative promoter usage

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9JLC6	Thyrotroph embryonic factor		RAT		301	UniRef90_P41224
Thyrotroph embryonic factor		HUMAN		303
TEF, PAR bZIP transcription factor		CANLF		311
TEF, PAR bZIP transcription factor		BOVIN		310
Thyrotroph embryonic factor isoform 1 (Fragment)		SHEEP		52
+112
Q9JLC6-2	Isoform 2 of Thyrotroph embryonic factor		MOUSE		273	UniRef90_Q9JLC6-2
TEF, PAR bZIP transcription factor		CANLF		347
Uncharacterized protein		PROCO		288
Uncharacterized protein		PAPAN		287
TEF isoform 2		PANTR		273
+92
Q9JLC6-3	Isoform Beta of Thyrotroph embryonic factor		MOUSE		285	UniRef90_Q9JLC6-2
TEF, PAR bZIP transcription factor		CANLF		347
Uncharacterized protein		PROCO		288
Uncharacterized protein		PAPAN		287
TEF isoform 2		PANTR		273
+92

Protein	Similar proteins		Species	Score	Length	Source
Q9JLC6	Thyrotroph embryonic factor		RAT		301	UniRef50_P41224
Thyrotroph embryonic factor		HUMAN		303
TEF, PAR bZIP transcription factor		CANLF		311
TEF, PAR bZIP transcription factor		BOVIN		310
Thyrotroph embryonic factor isoform 1 (Fragment)		SHEEP		52
+147
Q9JLC6-2	Transcription factor VBP		CHICK		319	UniRef50_Q92172
Thyrotroph embryonic factor		Patagioenas fasciata monilis		319
Transcription factor VBP		Bengalese finch		270
TEF, PAR bZIP transcription factor		RABIT		346
Uncharacterized protein		SARHA		285
+160
Q9JLC6-3	Transcription factor VBP		CHICK		319	UniRef50_Q92172
Thyrotroph embryonic factor		Patagioenas fasciata monilis		319
Transcription factor VBP		Bengalese finch		270
TEF, PAR bZIP transcription factor		RABIT		346
Uncharacterized protein		SARHA		285
+160

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF194420 mRNA Translation: AAF70830.1 AY540631 mRNA Translation: AAS45599.1 AY540632 mRNA Translation: AAS45600.1 AK075601 mRNA Translation: BAC35849.1 AK141606 mRNA Translation: BAE24760.1 AK145125 mRNA Translation: BAE26249.1 AK147965 mRNA Translation: BAE28255.1 AK154472 mRNA Translation: BAE32609.1 BC017689 mRNA Translation: AAH17689.1 BC036982 mRNA Translation: AAH36982.1
CCDSⁱ	CCDS27671.2 [Q9JLC6-2] CCDS27672.1 [Q9JLC6-1]
RefSeqⁱ	NP_059072.1, NM_017376.3 [Q9JLC6-1] NP_705617.2, NM_153484.3 [Q9JLC6-2]
UniGeneⁱ	Mm.270278

3D structure databases

ProteinModelPortalⁱ	Q9JLC6
SMRⁱ	Q9JLC6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	204106, 3 interactors
STRINGⁱ	10090.ENSMUSP00000023024

PTM databases

iPTMnetⁱ	Q9JLC6
PhosphoSitePlusⁱ	Q9JLC6

Proteomic databases

PaxDbⁱ	Q9JLC6
PRIDEⁱ	Q9JLC6

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000023024; ENSMUSP00000023024; ENSMUSG00000022389 [Q9JLC6-1] ENSMUST00000109553; ENSMUSP00000105180; ENSMUSG00000022389 [Q9JLC6-2]
GeneIDⁱ	21685
KEGGⁱ	mmu:21685
UCSCⁱ	uc007wxj.2 mouse [Q9JLC6-2] uc007wxk.2 mouse [Q9JLC6-1]

Organism-specific databases

CTDⁱ	7008
MGIⁱ	MGI:98663 Tef

Phylogenomic databases

eggNOGⁱ	KOG3119 Eukaryota ENOG410YJ8G LUCA
GeneTreeⁱ	ENSGT00440000039070
HOVERGENⁱ	HBG004983
InParanoidⁱ	Q9JLC6
KOⁱ	K09058
OMAⁱ	SKYETRY
OrthoDBⁱ	EOG091G0EO0
PhylomeDBⁱ	Q9JLC6
TreeFamⁱ	TF315869

Miscellaneous databases

ChiTaRSⁱ	Tef mouse
Protein Ontology More...PROⁱ	PR:Q9JLC6
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000022389 Expressed in 288 organ(s), highest expression level in cerebellum
CleanExⁱ	MM_TEF
ExpressionAtlasⁱ	Q9JLC6 baseline and differential
Genevisibleⁱ	Q9JLC6 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR029832 TEF/VBP
PANTHERⁱ	PTHR11988:SF24 PTHR11988:SF24, 1 hit
Pfamⁱ	View protein in Pfam PF07716 bZIP_2, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TEF_MOUSE
Accessionⁱ	Q9JLC6Primary (citable) accession number: Q9JLC6 Secondary accession number(s): Q3U426 Q8VD02
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
	Last sequence update:	October 1, 2000
	Last modified:	September 12, 2018
	This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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UniParc

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UniRef

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Annotation systems

Supporting data

UniProtKB - Q9JLC6 (TEF_MOUSE)

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Thyrotroph embryonic factor

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ