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Protein

C-type lectin domain family 1 member B

Gene

Clec1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

C-type lectin-like receptor that functions as a platelet receptor for the lymphatic endothelial marker, PDPN. After ligand activation, signals via sequential activation of SRC and SYK tyrosine kinases leading to activation of PLCG2.By similarity

Miscellaneous

Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2 (PubMed:16174766).1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell surface receptor signaling pathway Source: MGI
  • platelet formation Source: MGI
  • signal transduction by protein phosphorylation Source: UniProtKB

Keywordsi

Molecular functionReceptor
LigandLectin

Enzyme and pathway databases

ReactomeiR-MMU-114604 GPVI-mediated activation cascade

Names & Taxonomyi

Protein namesi
Recommended name:
C-type lectin domain family 1 member B
Alternative name(s):
C-type lectin-like receptor 2
Short name:
CLEC-2
Gene namesi
Name:Clec1b
Synonyms:Clec2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1913287 Clec1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31CytoplasmicSequence analysisAdd BLAST31
Transmembranei32 – 52Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini53 – 229ExtracellularSequence analysisAdd BLAST177

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002800441 – 229C-type lectin domain family 1 member BAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7Phosphotyrosine1 Publication1
Glycosylationi67N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi102 ↔ 113PROSITE-ProRule annotation
Glycosylationi120N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi130 ↔ 216PROSITE-ProRule annotation
Glycosylationi134N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi184N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi195 ↔ 208PROSITE-ProRule annotation

Post-translational modificationi

Glycosylated.1 Publication
Phosphorylated on tyrosine residue in response to rhodocytin binding.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9JL99
PRIDEiQ9JL99

PTM databases

iPTMnetiQ9JL99
PhosphoSitePlusiQ9JL99

Expressioni

Tissue specificityi

Hematopoietic cells, megakaryocytes and platelets.2 Publications

Gene expression databases

BgeeiENSMUSG00000030159 Expressed in 108 organ(s), highest expression level in blood
CleanExiMM_CLEC1B
ExpressionAtlasiQ9JL99 baseline and differential
GenevisibleiQ9JL99 MM

Interactioni

Subunit structurei

Homodimer. Interacts (via cytoplasmic domain) with RACK1; promotes CLEC1B ubiquitination and proteasome-mediated degradation. Interacts (dimer) with SYK (via SH2 domains) (By similarity). Interacts with PDPN; the interaction is independent of CLEC1B glycosylation and activates CLEC1B (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032262

Structurei

3D structure databases

ProteinModelPortaliQ9JL99
SMRiQ9JL99
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 217C-type lectinPROSITE-ProRule annotationAdd BLAST109

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297 Eukaryota
ENOG410XPJ1 LUCA
GeneTreeiENSGT00700000104266
HOGENOMiHOG000111777
HOVERGENiHBG094936
InParanoidiQ9JL99
KOiK10070
OMAiEVWKWED
OrthoDBiEOG091G0OAQ
PhylomeDBiQ9JL99
TreeFamiTF336674

Family and domain databases

CDDicd03593 CLECT_NK_receptors_like, 1 hit
Gene3Di3.10.100.10, 1 hit
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR016187 CTDL_fold
IPR033992 NKR-like_CTLD
PfamiView protein in Pfam
PF00059 Lectin_C, 1 hit
SMARTiView protein in SMART
SM00034 CLECT, 1 hit
SUPFAMiSSF56436 SSF56436, 1 hit
PROSITEiView protein in PROSITE
PS50041 C_TYPE_LECTIN_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9JL99-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MQDEDGYITL NIKPRKQALS SAEPASSWWR VMALVLLISS MGLVVGLVAL
60 70 80 90 100
GIMSVTQQKY LLAEKENLSA TLQQLAKKFC QELIRQSEIK TKSTFEHKCS
110 120 130 140 150
PCATKWRYHG DSCYGFFRRN LTWEESKQYC TEQNATLVKT ASQSTLDYIA
160 170 180 190 200
ERITSVRWIG LSRQNSKKDW MWEDSSVLRK NGINLSGNTE ENMNCAYLHN
210 220
GKIHPASCKE RHYLICERNA GMTRVDQLL
Length:229
Mass (Da):26,239
Last modified:October 1, 2000 - v1
Checksum:i1EC9377F491CA52B
GO
Isoform 2 (identifier: Q9JL99-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     23-54: Missing.

Show »
Length:197
Mass (Da):22,870
Checksum:iE0CBDEEFE2441A5F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0T1G3A0T1G3_MOUSE
C-type lectin domain family 1 membe...
Clec1b
83Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02351623 – 54Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF201457 mRNA Translation: AAF36831.1
EF070191 mRNA Translation: ABK60036.1
BC064054 mRNA Translation: AAH64054.1
CCDSiCCDS20586.1 [Q9JL99-1]
CCDS57452.1 [Q9JL99-2]
RefSeqiNP_001191168.1, NM_001204239.1 [Q9JL99-2]
NP_064369.1, NM_019985.3 [Q9JL99-1]
UniGeneiMm.30700

Genome annotation databases

EnsembliENSMUST00000032262; ENSMUSP00000032262; ENSMUSG00000030159 [Q9JL99-1]
ENSMUST00000112081; ENSMUSP00000107712; ENSMUSG00000030159 [Q9JL99-2]
GeneIDi56760
KEGGimmu:56760
UCSCiuc009efo.2 mouse [Q9JL99-1]
uc009efp.2 mouse [Q9JL99-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF201457 mRNA Translation: AAF36831.1
EF070191 mRNA Translation: ABK60036.1
BC064054 mRNA Translation: AAH64054.1
CCDSiCCDS20586.1 [Q9JL99-1]
CCDS57452.1 [Q9JL99-2]
RefSeqiNP_001191168.1, NM_001204239.1 [Q9JL99-2]
NP_064369.1, NM_019985.3 [Q9JL99-1]
UniGeneiMm.30700

3D structure databases

ProteinModelPortaliQ9JL99
SMRiQ9JL99
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032262

PTM databases

iPTMnetiQ9JL99
PhosphoSitePlusiQ9JL99

Proteomic databases

PaxDbiQ9JL99
PRIDEiQ9JL99

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032262; ENSMUSP00000032262; ENSMUSG00000030159 [Q9JL99-1]
ENSMUST00000112081; ENSMUSP00000107712; ENSMUSG00000030159 [Q9JL99-2]
GeneIDi56760
KEGGimmu:56760
UCSCiuc009efo.2 mouse [Q9JL99-1]
uc009efp.2 mouse [Q9JL99-2]

Organism-specific databases

CTDi51266
MGIiMGI:1913287 Clec1b

Phylogenomic databases

eggNOGiKOG4297 Eukaryota
ENOG410XPJ1 LUCA
GeneTreeiENSGT00700000104266
HOGENOMiHOG000111777
HOVERGENiHBG094936
InParanoidiQ9JL99
KOiK10070
OMAiEVWKWED
OrthoDBiEOG091G0OAQ
PhylomeDBiQ9JL99
TreeFamiTF336674

Enzyme and pathway databases

ReactomeiR-MMU-114604 GPVI-mediated activation cascade

Miscellaneous databases

PROiPR:Q9JL99
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030159 Expressed in 108 organ(s), highest expression level in blood
CleanExiMM_CLEC1B
ExpressionAtlasiQ9JL99 baseline and differential
GenevisibleiQ9JL99 MM

Family and domain databases

CDDicd03593 CLECT_NK_receptors_like, 1 hit
Gene3Di3.10.100.10, 1 hit
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR016187 CTDL_fold
IPR033992 NKR-like_CTLD
PfamiView protein in Pfam
PF00059 Lectin_C, 1 hit
SMARTiView protein in SMART
SM00034 CLECT, 1 hit
SUPFAMiSSF56436 SSF56436, 1 hit
PROSITEiView protein in PROSITE
PS50041 C_TYPE_LECTIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLC1B_MOUSE
AccessioniPrimary (citable) accession number: Q9JL99
Secondary accession number(s): A0T1G2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: October 1, 2000
Last modified: September 12, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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