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Protein

Interferon-stimulated gene 20 kDa protein

Gene

Isg20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. Exhibits antiviral activity against RNA viruses in an exonuclease-dependent manner. May also play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis (By similarity).By similarity

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

Cofactori

Mn2+Note: Binds 2 manganese ions per subunit.

pH dependencei

Optimum pH is 7.0.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi11Manganese 1By similarity1
Metal bindingi13Manganese 1By similarity1
Metal bindingi90Manganese 2By similarity1
Metal bindingi93Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease, RNA-binding
Biological processAntiviral defense, Immunity, Innate immunity, rRNA processing
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-stimulated gene 20 kDa protein (EC:3.1.13.1)
Alternative name(s):
Promyelocytic leukemia nuclear body-associated protein ISG20
Protein DnaQL
Gene namesi
Name:Isg20
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1928895 Isg20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000842441 – 300Interferon-stimulated gene 20 kDa proteinAdd BLAST300

Proteomic databases

PaxDbiQ9JL16
PRIDEiQ9JL16

PTM databases

iPTMnetiQ9JL16
PhosphoSitePlusiQ9JL16

Expressioni

Inductioni

Induced by interferons alpha and beta. Weaker induction was seen with interferon gamma. Increased expression was seen at the transcriptional level (By similarity).By similarity

Gene expression databases

BgeeiENSMUSG00000039236 Expressed in 132 organ(s), highest expression level in blood
CleanExiMM_ISG20
ExpressionAtlasiQ9JL16 baseline and differential
GenevisibleiQ9JL16 MM

Interactioni

Subunit structurei

Associates with PML and SP100 in the PML NB complex. Associates with survival motor neuron protein (SMN)-containing macromolecular nuclear complexes and U1 and U2 snRNAs and U3 snoRNA (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040080

Structurei

3D structure databases

ProteinModelPortaliQ9JL16
SMRiQ9JL16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the exonuclease superfamily.Curated

Phylogenomic databases

eggNOGiKOG2249 Eukaryota
COG0847 LUCA
GeneTreeiENSGT00520000055542
HOGENOMiHOG000182422
InParanoidiQ9JL16
KOiK12579
OMAiARCSLVD
OrthoDBiEOG091G0I6S
PhylomeDBiQ9JL16
TreeFamiTF354340

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR013520 Exonuclease_RNaseT/DNA_pol3
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00929 RNase_T, 1 hit
SMARTiView protein in SMART
SM00479 EXOIII, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9JL16-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGIPEVVAM DCEMVGLGPQ RVSGLARCSI VNIHGAVLYD KYIRPEGEIT
60 70 80 90 100
DYRTQVSGVT PQHMVRATPF GEARLEILQL LKGKLVVGHD LKHDFNALKE
110 120 130 140 150
DMSKYTIYDT STDRLLWHEA KLQYYSRVSL RLLCKRLLHK NIQVLPGSLL
160 170 180 190 200
GVGGCILPGT DILHLLLYVG MVRIADLRLL TPFLPPSCLA CPLLPESLAS
210 220 230 240 250
ARSHAVISAL SSSSHLLTPL PNPSQGPQGH VDRLSGQLQD WGGSPLAPAL
260 270 280 290 300
PVSAEQLAGP LLCGRCQGHN GALQNLSATQ SPARAALPWD VRLNFILIQG
Length:300
Mass (Da):32,503
Last modified:October 1, 2000 - v1
Checksum:i3517E33DBFD8D463
GO
Isoform 2 (identifier: Q9JL16-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     144-181: VLPGSLLGVG...VRIADLRLLT → NNWRGHCSVE...RGLPCPGTSD
     182-300: Missing.

Show »
Length:181
Mass (Da):20,583
Checksum:iE448E90834AC5594
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RP59A0A0U1RP59_MOUSE
Interferon-stimulated gene 20 kDa p...
Isg20 mCG_6303
145Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012431144 – 181VLPGS…LRLLT → NNWRGHCSVEDARATMELYK ISQRLRAQRGLPCPGTSD in isoform 2. 2 PublicationsAdd BLAST38
Alternative sequenceiVSP_012432182 – 300Missing in isoform 2. 2 PublicationsAdd BLAST119

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217484 mRNA Translation: AAF59917.1
AK008139 mRNA Translation: BAB25487.1
AK008171 mRNA Translation: BAB25509.1
AK008353 mRNA Translation: BAB25623.1
BC022751 mRNA Translation: AAH22751.1
CCDSiCCDS21376.1 [Q9JL16-2]
CCDS71982.1 [Q9JL16-1]
RefSeqiNP_001106999.1, NM_001113527.1 [Q9JL16-2]
NP_001278149.1, NM_001291220.1 [Q9JL16-1]
NP_001278150.1, NM_001291221.1 [Q9JL16-2]
NP_065608.2, NM_020583.5 [Q9JL16-2]
XP_006541089.1, XM_006541026.2 [Q9JL16-1]
XP_006541091.1, XM_006541028.3 [Q9JL16-1]
UniGeneiMm.322843

Genome annotation databases

EnsembliENSMUST00000038142; ENSMUSP00000040080; ENSMUSG00000039236 [Q9JL16-2]
ENSMUST00000118867; ENSMUSP00000112480; ENSMUSG00000039236 [Q9JL16-2]
ENSMUST00000120331; ENSMUSP00000113255; ENSMUSG00000039236 [Q9JL16-2]
ENSMUST00000121645; ENSMUSP00000112621; ENSMUSG00000039236 [Q9JL16-1]
GeneIDi57444
KEGGimmu:57444
UCSCiuc009hxt.2 mouse [Q9JL16-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217484 mRNA Translation: AAF59917.1
AK008139 mRNA Translation: BAB25487.1
AK008171 mRNA Translation: BAB25509.1
AK008353 mRNA Translation: BAB25623.1
BC022751 mRNA Translation: AAH22751.1
CCDSiCCDS21376.1 [Q9JL16-2]
CCDS71982.1 [Q9JL16-1]
RefSeqiNP_001106999.1, NM_001113527.1 [Q9JL16-2]
NP_001278149.1, NM_001291220.1 [Q9JL16-1]
NP_001278150.1, NM_001291221.1 [Q9JL16-2]
NP_065608.2, NM_020583.5 [Q9JL16-2]
XP_006541089.1, XM_006541026.2 [Q9JL16-1]
XP_006541091.1, XM_006541028.3 [Q9JL16-1]
UniGeneiMm.322843

3D structure databases

ProteinModelPortaliQ9JL16
SMRiQ9JL16
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040080

PTM databases

iPTMnetiQ9JL16
PhosphoSitePlusiQ9JL16

Proteomic databases

PaxDbiQ9JL16
PRIDEiQ9JL16

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038142; ENSMUSP00000040080; ENSMUSG00000039236 [Q9JL16-2]
ENSMUST00000118867; ENSMUSP00000112480; ENSMUSG00000039236 [Q9JL16-2]
ENSMUST00000120331; ENSMUSP00000113255; ENSMUSG00000039236 [Q9JL16-2]
ENSMUST00000121645; ENSMUSP00000112621; ENSMUSG00000039236 [Q9JL16-1]
GeneIDi57444
KEGGimmu:57444
UCSCiuc009hxt.2 mouse [Q9JL16-1]

Organism-specific databases

CTDi3669
MGIiMGI:1928895 Isg20

Phylogenomic databases

eggNOGiKOG2249 Eukaryota
COG0847 LUCA
GeneTreeiENSGT00520000055542
HOGENOMiHOG000182422
InParanoidiQ9JL16
KOiK12579
OMAiARCSLVD
OrthoDBiEOG091G0I6S
PhylomeDBiQ9JL16
TreeFamiTF354340

Miscellaneous databases

PROiPR:Q9JL16
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039236 Expressed in 132 organ(s), highest expression level in blood
CleanExiMM_ISG20
ExpressionAtlasiQ9JL16 baseline and differential
GenevisibleiQ9JL16 MM

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR013520 Exonuclease_RNaseT/DNA_pol3
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00929 RNase_T, 1 hit
SMARTiView protein in SMART
SM00479 EXOIII, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiISG20_MOUSE
AccessioniPrimary (citable) accession number: Q9JL16
Secondary accession number(s): Q9CPZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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