UniProtKB - Q9JKV2 (CILK1_MOUSE)
Serine/threonine-protein kinase ICK
Cilk1
Functioni
Has an essential role in ciliogenesis, particularly in neuronal and retinal progenitor cells (PubMed:24797473).
Phosphorylates KIF3A (PubMed:24797473).
Involved in the control of ciliary length (PubMed:24853502).
Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (PubMed:24797473, PubMed:24853502).
May play a role in cardiac development (By similarity).
Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling -dependent manner and this regulation requires its kinase activity (PubMed:25243405).
By similarity3 PublicationsCatalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | ATPPROSITE-ProRule annotationBy similarity | 1 | |
Active sitei | 125 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 18 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- protein kinase activity Source: MGI
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: MGI
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cilium assembly Source: UniProtKB
- intracellular signal transduction Source: UniProtKB
- intraciliary anterograde transport Source: UniProtKB
- intraciliary retrograde transport Source: UniProtKB
- intraciliary transport Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- signal transduction Source: MGI
Keywordsi
Molecular function | Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cilium biogenesis/degradation |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase ICK (EC:2.7.11.11 Publication)Alternative name(s): Intestinal cell kinase Short name: mICK MAK-related kinase Short name: MRK |
Gene namesi | Name:Cilk1 Synonyms:Ick |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1934157, Cilk1 |
VEuPathDBi | HostDB:ENSMUSG00000009828 |
Subcellular locationi
Cytoskeleton
- cilium basal body 1 Publication
Cytoplasm and Cytosol
- cytosol By similarity
Nucleus
- Nucleus 2 Publications
Other locations
- cilium 2 Publications
Note: Also found at the ciliary tip (PubMed:24797473). Predominant nuclear localization has been observed with a N-terminally GFP-tagged construct in transfected COS-7 cells (PubMed:15988018).2 Publications
Cytoskeleton
- ciliary basal body Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB-SubCell
Nucleus
- fibrillar center Source: MGI
- nucleus Source: UniProtKB
Other locations
- ciliary base Source: UniProtKB
- ciliary tip Source: UniProtKB
- cilium Source: UniProtKB
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 33 | K → M: No effect on cilium length. 1 Publication | 1 | |
Mutagenesisi | 33 | K → R: Loss of kinase activity; no effect on nuclear subcellular location. 1 Publication | 1 | |
Mutagenesisi | 157 – 159 | TDY → ADF: Loss of kinase activity and of autophosphorylation; loss of phosphorylation by CDK7; no effect on nuclear subcellular location. 1 Publication | 3 | |
Mutagenesisi | 157 | T → A: Loss of kinase activity; loss of phosphorylation by CDK7; no effect on autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 159 | Y → F: Loss of kinase activity and of autophosphorylation; no effect on phosphorylation by CDK7. 1 Publication | 1 | |
Mutagenesisi | 169 | E → A: Complete loss of kinase activity and of nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 184 | W → A: Complete loss of kinase activity and of nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 270 | K → A: Partial loss of autocatalytic kinase activity. Complete loss of kinase activity and of nuclear localization; when associated with A-271 and A-272. 1 Publication | 1 | |
Mutagenesisi | 271 | K → A: Partial loss of kinase activity. Complete loss of kinase activity and of nuclear localization; when associated with A-270 and A-272. 1 Publication | 1 | |
Mutagenesisi | 272 | R → A: Complete loss of kinase activity and of nuclear localization. Complete loss of kinase activity and of nuclear localization; when associated with A-270 and A-272. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086008 | 1 – 629 | Serine/threonine-protein kinase ICKAdd BLAST | 629 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 157 | Phosphothreonine; by CDK7Combined sources1 Publication | 1 | |
Modified residuei | 159 | PhosphotyrosineCombined sources1 Publication | 1 | |
Modified residuei | 161 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q9JKV2 |
MaxQBi | Q9JKV2 |
PaxDbi | Q9JKV2 |
PRIDEi | Q9JKV2 |
ProteomicsDBi | 267186 |
PTM databases
iPTMneti | Q9JKV2 |
PhosphoSitePlusi | Q9JKV2 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000009828, Expressed in trigeminal ganglion and 278 other tissues |
ExpressionAtlasi | Q9JKV2, baseline and differential |
Genevisiblei | Q9JKV2, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000048234 |
Miscellaneous databases
RNActi | Q9JKV2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 284 | Protein kinasePROSITE-ProRule annotationCuratedAdd BLAST | 281 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 292 – 376 | DisorderedSequence analysisAdd BLAST | 85 | |
Regioni | 455 – 483 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 581 – 629 | DisorderedSequence analysisAdd BLAST | 49 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 328 – 343 | Polar residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0661, Eukaryota |
GeneTreei | ENSGT00940000158807 |
InParanoidi | Q9JKV2 |
OMAi | DWASKYP |
OrthoDBi | 76933at2759 |
PhylomeDBi | Q9JKV2 |
TreeFami | TF328769 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MNRYTTIKQL GDGTYGSVLL GRSIESGELI AIKKMKRKFY SWEECMNLRE
60 70 80 90 100
VKSLKKLNHA NIVKLKEVIR ENDHLYFIFE YMKENLYQLI KERNKLFPES
110 120 130 140 150
AIRNIMYQIL QGLAFIHKHG FFHRDLKPEN LLCMGPELVK IADFGLAREI
160 170 180 190 200
RSRPPYTDYV STRWYRAPEV LLRSTNYSSP IDIWAVGCIM AEVYTLRPLF
210 220 230 240 250
PGASEIDTIF KICQVLGTPK KTDWPEGYQL SSAMNFLWPQ CIPNNLKTLI
260 270 280 290 300
PNASSEAIQL LRDLLQWDPK KRPTASQALR YPYFQIGHPL GIISKDSGKP
310 320 330 340 350
QREVQDKTGP PPYIKPAPPA QAPAKAYTLI SSRPSQASQP PQHSVHPYKG
360 370 380 390 400
DVSRTEQLSH VQEGKPSPPF FPSLHNKNLQ PKILASLEQK NGEIKPKSRR
410 420 430 440 450
RWGLISRSTK GSDDWADLDD LDFSPSLTRI DVKNKKRQSD DTLCRFESVL
460 470 480 490 500
DLKPSESVGT GTTVSTQASS QRRDTPTLQS SAKQHYLKHS RYLPGINIRN
510 520 530 540 550
GVLPNPGKDF LPSNSWSSSG LSGKSSGTVS VVSKITSVGS GSASSSGLTG
560 570 580 590 600
SYIPSFLKKE IGSVMQRVQL APLAAPPPGY SSLKAVRPHP GRPFFHTQPR
610 620
STPGLIPRPP AAQPVHGRID WSSKYPSRR
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3UZZ3 | Q3UZZ3_MOUSE | Serine/threonine-protein kinase ICK | Cilk1 Ick | 539 | Annotation score: | ||
D3YZV1 | D3YZV1_MOUSE | Serine/threonine-protein kinase ICK | Cilk1 Ick | 28 | Annotation score: | ||
Q9D7Q7 | Q9D7Q7_MOUSE | Serine/threonine-protein kinase ICK | Cilk1 Ick | 100 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 533 | S → R in AAF37277 (PubMed:10699974).Curated | 1 | |
Sequence conflicti | 616 | H → L in AAF37277 (PubMed:10699974).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF225918 mRNA Translation: AAF37277.1 BC028863 mRNA Translation: AAH28863.1 |
CCDSi | CCDS40697.1 |
RefSeqi | NP_001157252.1, NM_001163780.1 NP_064371.2, NM_019987.2 XP_006511367.1, XM_006511304.2 |
Genome annotation databases
Ensembli | ENSMUST00000044551; ENSMUSP00000048234; ENSMUSG00000009828 ENSMUST00000118869; ENSMUSP00000112961; ENSMUSG00000009828 |
GeneIDi | 56542 |
KEGGi | mmu:56542 |
UCSCi | uc009qtt.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF225918 mRNA Translation: AAF37277.1 BC028863 mRNA Translation: AAH28863.1 |
CCDSi | CCDS40697.1 |
RefSeqi | NP_001157252.1, NM_001163780.1 NP_064371.2, NM_019987.2 XP_006511367.1, XM_006511304.2 |
3D structure databases
SMRi | Q9JKV2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000048234 |
PTM databases
iPTMneti | Q9JKV2 |
PhosphoSitePlusi | Q9JKV2 |
Proteomic databases
jPOSTi | Q9JKV2 |
MaxQBi | Q9JKV2 |
PaxDbi | Q9JKV2 |
PRIDEi | Q9JKV2 |
ProteomicsDBi | 267186 |
Protocols and materials databases
ABCDi | Q9JKV2, 1 sequenced antibody |
Antibodypediai | 605, 298 antibodies from 24 providers |
DNASUi | 56542 |
Genome annotation databases
Ensembli | ENSMUST00000044551; ENSMUSP00000048234; ENSMUSG00000009828 ENSMUST00000118869; ENSMUSP00000112961; ENSMUSG00000009828 |
GeneIDi | 56542 |
KEGGi | mmu:56542 |
UCSCi | uc009qtt.2, mouse |
Organism-specific databases
CTDi | 22858 |
MGIi | MGI:1934157, Cilk1 |
VEuPathDBi | HostDB:ENSMUSG00000009828 |
Phylogenomic databases
eggNOGi | KOG0661, Eukaryota |
GeneTreei | ENSGT00940000158807 |
InParanoidi | Q9JKV2 |
OMAi | DWASKYP |
OrthoDBi | 76933at2759 |
PhylomeDBi | Q9JKV2 |
TreeFami | TF328769 |
Miscellaneous databases
BioGRID-ORCSi | 56542, 3 hits in 64 CRISPR screens |
PROi | PR:Q9JKV2 |
RNActi | Q9JKV2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000009828, Expressed in trigeminal ganglion and 278 other tissues |
ExpressionAtlasi | Q9JKV2, baseline and differential |
Genevisiblei | Q9JKV2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CILK1_MOUSE | |
Accessioni | Q9JKV2Primary (citable) accession number: Q9JKV2 Secondary accession number(s): Q8K138 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | June 7, 2004 | |
Last modified: | February 23, 2022 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families