UniProtKB - Q9JKS4 (LDB3_MOUSE)
LIM domain-binding protein 3
Ldb3
Functioni
GO - Molecular functioni
- actin binding Source: GO_Central
- cytoskeletal protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- muscle alpha-actinin binding Source: MGI
- protein kinase C binding Source: UniProtKB
GO - Biological processi
- actin cytoskeleton organization Source: GO_Central
- heart development Source: GO_Central
- muscle structure development Source: GO_Central
- sarcomere organization Source: MGI
Keywordsi
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: LIM domain-binding protein 3Alternative name(s): Protein cypher Protein oracle Z-band alternatively spliced PDZ-motif protein |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1344412, Ldb3 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Other locations
- perinuclear region 1 Publication
- pseudopodium 1 Publication
- Z line 1 Publication
Note: Localized to the cytoplasm around nuclei and pseudopodia of undifferentiated cells and detected throughout the myotubes of differentiated cells. Colocalizes with ACTN2 at the Z-lines.
Cytoskeleton
- cytoskeleton Source: UniProtKB
- filamentous actin Source: GO_Central
- stress fiber Source: GO_Central
Other locations
- adherens junction Source: GO_Central
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- pseudopodium Source: UniProtKB-SubCell
- Z disc Source: MGI
Keywords - Cellular componenti
Cell projection, Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075768 | 1 – 723 | LIM domain-binding protein 3Add BLAST | 723 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 44 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 98 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 119 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 121 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 123 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 214 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 216 | Omega-N-methylarginineCombined sources | 1 | ||
Modified residuei | 220 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 251 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 512 | Omega-N-methylarginineCombined sources | 1 | ||
Modified residuei | 529 | Omega-N-methylarginineCombined sources | 1 | ||
Isoform 21 Publication (identifier: Q9JKS4-2) | |||||
Modified residuei | 112 | PhosphoserineCombined sources | 1 | ||
Isoform 41 Publication (identifier: Q9JKS4-4) | |||||
Modified residuei | 112 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 291 | Omega-N-methylarginineCombined sources | 1 | ||
Isoform 62 Publications (identifier: Q9JKS4-6) | |||||
Modified residuei | 112 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 288 | PhosphoserineCombined sources | 1 | ||
Isoform 51 Publication (identifier: Q9JKS4-5) | |||||
Modified residuei | 327 | PhosphoserineCombined sources | 1 | ||
Isoform 32 Publications (identifier: Q9JKS4-3) | |||||
Modified residuei | 330 | Omega-N-methylarginineCombined sources | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
PaxDbi | Q9JKS4 |
PeptideAtlasi | Q9JKS4 |
PRIDEi | Q9JKS4 |
PTM databases
iPTMneti | Q9JKS4 |
PhosphoSitePlusi | Q9JKS4 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000021798, Expressed in gastrocnemius medialis and 284 other tissues |
ExpressionAtlasi | Q9JKS4, baseline and differential |
Genevisiblei | Q9JKS4, MM |
Interactioni
Subunit structurei
Interacts via its LIM domains with various PKC isoforms.
Interacts via its PDZ domain with the ACTN2 C-terminal region.
Interacts with MYOZ1, MYOZ2 and MYOZ3.
By similarity1 PublicationGO - Molecular functioni
- actin binding Source: GO_Central
- cytoskeletal protein binding Source: MGI
- muscle alpha-actinin binding Source: MGI
- protein kinase C binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 204912, 17 interactors |
IntActi | Q9JKS4, 4 interactors |
MINTi | Q9JKS4 |
STRINGi | 10090.ENSMUSP00000022327 |
Miscellaneous databases
RNActi | Q9JKS4, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q9JKS4 |
SMRi | Q9JKS4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9JKS4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 84 | PDZPROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 545 – 603 | LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST | 59 | |
Domaini | 604 – 663 | LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 664 – 723 | LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST | 60 |
Keywords - Domaini
LIM domain, RepeatPhylogenomic databases
eggNOGi | KOG1703, Eukaryota |
GeneTreei | ENSGT00940000154877 |
HOGENOMi | CLU_038114_0_0_1 |
InParanoidi | Q9JKS4 |
OMAi | SNVYCER |
OrthoDBi | 1013114at2759 |
PhylomeDBi | Q9JKS4 |
TreeFami | TF106408 |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR031847, DUF4749 IPR001478, PDZ IPR036034, PDZ_sf IPR006643, Zasp-like_motif IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF15936, DUF4749, 1 hit PF00412, LIM, 3 hits PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM00132, LIM, 3 hits SM00228, PDZ, 1 hit SM00735, ZM, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 3 hits PS50106, PDZ, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSYSVTLTGP GPWGFRLQGG KDFNMPLTIS RITPGSKAAQ SQLSQGDLVV
60 70 80 90 100
AIDGVNTDTM THLEAQNKIK SASYNLSLTL QKSKRPIPIS TTAPPIQSPL
110 120 130 140 150
PVIPHQKDPA LDTNGSLATP SPSPEARASP GALEFGDTFS SSFSQTSVCS
160 170 180 190 200
PLMEASGPVL PLGSPVAKAS SEGAQGSVSP KVLPGPSQPR QYNNPIGLYS
210 220 230 240 250
AETLREMAQM YQMSLRGKAS GAGLLGGSLP VKDLAVDSAS PVYQAVIKTQ
260 270 280 290 300
SKPEDEADEW ARRSSNLQSR SFRILAQMTG TEYMQDPDEE ALRRSSTPIE
310 320 330 340 350
HAPVCTSQAT SPLLPASAQS PAAASPIAAS PTLATAAATH AAAASAAGPA
360 370 380 390 400
ASPVENPRPQ ASAYSPAAAA SPAPSAHTSY SEGPAAPAPK PRVVTTASIR
410 420 430 440 450
PSVYQPVPAS SYSPSPGANY SPTPYTPSPA PAYTPSPAPT YTPSPAPTYS
460 470 480 490 500
PSPAPAYTPS PAPNYTPTPS AAYSGGPSES ASRPPWVTDD SFSQKFAPGK
510 520 530 540 550
STTTVSKQTL PRGAPAYNPT GPQVTPLARG TFQRAERFPA SSRTPLCGHC
560 570 580 590 600
NNVIRGPFLV AMGRSWHPEE FNCAYCKTSL ADVCFVEEQN NVYCERCYEQ
610 620 630 640 650
FFAPICAKCN TKIMGEVMHA LRQTWHTTCF VCAACKKPFG NSLFHMEDGE
660 670 680 690 700
PYCEKDYINL FSTKCHGCDF PVEAGDKFIE ALGHTWHDTC FICAVCHVNL
710 720
EGQPFYSKKD KPLCKKHAHA INV
The sequence of this isoform differs from the canonical sequence as follows:
108-227: DPALDTNGSL...KASGAGLLGG → VVANSPANAD...SDFSGASPLA
296-327: STPIEHAPVCTSQATSPLLPASAQSPAAASPI → RERFETERNSPRFAKLRNWHHGLSAQILNVKS
328-723: Missing.
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9PYJ9 | E9PYJ9_MOUSE | LIM domain-binding protein 3 | Ldb3 | 679 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 108 – 145 | DPALD…SSFSQ → VVANSPANADYQERFNPSVL KGLSSVLKGLSSVHPQAH in BAB23128 (PubMed:16141072).CuratedAdd BLAST | 38 | ||
Sequence conflicti | 450 | S → T in AAD42950 (PubMed:10391924).Curated | 1 | ||
Sequence conflicti | 450 | S → T in AAO26188 (PubMed:12499364).Curated | 1 | ||
Sequence conflicti | 450 | S → T in AAO26189 (PubMed:12499364).Curated | 1 | ||
Sequence conflicti | 512 | R → W in BAD32258 (PubMed:15368895).Curated | 1 | ||
Isoform 21 Publication (identifier: Q9JKS4-2) | |||||
Sequence conflicti | 176 – 188 | AQGSD…ASPLA → AQGSDFSG in AAO26189 (PubMed:12499364).CuratedAdd BLAST | 13 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_051903 | 108 – 227 | DPALD…GLLGG → VVANSPANADYQERFNPSVL KDSALSTHKPIEVKGLGGKA TIIHAQYNTPISMYSQDAIM DAIAGQAQAQGSDFSGASPL A in isoform 2, isoform 4 and isoform 6. 5 PublicationsAdd BLAST | 120 | |
Alternative sequenceiVSP_051904 | 296 – 357 | Missing in isoform 3 and isoform 4. 3 PublicationsAdd BLAST | 62 | |
Alternative sequenceiVSP_051905 | 296 – 327 | STPIE…AASPI → RERFETERNSPRFAKLRNWH HGLSAQILNVKS in isoform 5 and isoform 6. 5 PublicationsAdd BLAST | 32 | |
Alternative sequenceiVSP_051906 | 328 – 723 | Missing in isoform 5 and isoform 6. 5 PublicationsAdd BLAST | 396 |
Sequence databases
Genome annotation databases
Ensembli | ENSMUST00000022327; ENSMUSP00000022327; ENSMUSG00000021798 [Q9JKS4-1] ENSMUST00000022328; ENSMUSP00000022328; ENSMUSG00000021798 [Q9JKS4-3] ENSMUST00000022330; ENSMUSP00000022330; ENSMUSG00000021798 [Q9JKS4-5] ENSMUST00000090040; ENSMUSP00000087494; ENSMUSG00000021798 [Q9JKS4-2] ENSMUST00000227819; ENSMUSP00000154119; ENSMUSG00000021798 [Q9JKS4-6] ENSMUST00000228044; ENSMUSP00000154758; ENSMUSG00000021798 [Q9JKS4-4] |
GeneIDi | 24131 |
KEGGi | mmu:24131 |
UCSCi | uc007taz.1, mouse [Q9JKS4-2] uc007tba.1, mouse [Q9JKS4-4] uc007tbc.1, mouse [Q9JKS4-1] uc007tbd.1, mouse [Q9JKS4-3] uc007tbe.1, mouse [Q9JKS4-6] uc007tbf.1, mouse [Q9JKS4-5] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WJL | NMR | - | A | 1-83 | [»] | |
BMRBi | Q9JKS4 | |||||
SMRi | Q9JKS4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204912, 17 interactors |
IntActi | Q9JKS4, 4 interactors |
MINTi | Q9JKS4 |
STRINGi | 10090.ENSMUSP00000022327 |
PTM databases
iPTMneti | Q9JKS4 |
PhosphoSitePlusi | Q9JKS4 |
Proteomic databases
PaxDbi | Q9JKS4 |
PeptideAtlasi | Q9JKS4 |
PRIDEi | Q9JKS4 |
Protocols and materials databases
Antibodypediai | 15974, 246 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000022327; ENSMUSP00000022327; ENSMUSG00000021798 [Q9JKS4-1] ENSMUST00000022328; ENSMUSP00000022328; ENSMUSG00000021798 [Q9JKS4-3] ENSMUST00000022330; ENSMUSP00000022330; ENSMUSG00000021798 [Q9JKS4-5] ENSMUST00000090040; ENSMUSP00000087494; ENSMUSG00000021798 [Q9JKS4-2] ENSMUST00000227819; ENSMUSP00000154119; ENSMUSG00000021798 [Q9JKS4-6] ENSMUST00000228044; ENSMUSP00000154758; ENSMUSG00000021798 [Q9JKS4-4] |
GeneIDi | 24131 |
KEGGi | mmu:24131 |
UCSCi | uc007taz.1, mouse [Q9JKS4-2] uc007tba.1, mouse [Q9JKS4-4] uc007tbc.1, mouse [Q9JKS4-1] uc007tbd.1, mouse [Q9JKS4-3] uc007tbe.1, mouse [Q9JKS4-6] uc007tbf.1, mouse [Q9JKS4-5] |
Organism-specific databases
CTDi | 11155 |
MGIi | MGI:1344412, Ldb3 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1703, Eukaryota |
GeneTreei | ENSGT00940000154877 |
HOGENOMi | CLU_038114_0_0_1 |
InParanoidi | Q9JKS4 |
OMAi | SNVYCER |
OrthoDBi | 1013114at2759 |
PhylomeDBi | Q9JKS4 |
TreeFami | TF106408 |
Miscellaneous databases
BioGRID-ORCSi | 24131, 0 hits in 17 CRISPR screens |
ChiTaRSi | Ldb3, mouse |
EvolutionaryTracei | Q9JKS4 |
PROi | PR:Q9JKS4 |
RNActi | Q9JKS4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021798, Expressed in gastrocnemius medialis and 284 other tissues |
ExpressionAtlasi | Q9JKS4, baseline and differential |
Genevisiblei | Q9JKS4, MM |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR031847, DUF4749 IPR001478, PDZ IPR036034, PDZ_sf IPR006643, Zasp-like_motif IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF15936, DUF4749, 1 hit PF00412, LIM, 3 hits PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM00132, LIM, 3 hits SM00228, PDZ, 1 hit SM00735, ZM, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 3 hits PS50106, PDZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LDB3_MOUSE | |
Accessioni | Q9JKS4Primary (citable) accession number: Q9JKS4 Secondary accession number(s): B2RSB0 Q9WVH2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references