Ldb3

Functionⁱ

May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.1 Publication

GO - Molecular functionⁱ

actin binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
  Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
  Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
cytoskeletal protein binding Source: MGI
metal ion binding Source: UniProtKB-KW
muscle alpha-actinin binding
Source: MGI
Inferred from direct assayⁱ
- "Ablation of Cypher, a PDZ-LIM domain Z-line protein, causes a severe form of congenital myopathy."
  Zhou Q., Chu P.H., Huang C., Cheng C.F., Martone M.E., Knoll G., Shelton G.D., Evans S., Chen J.
  J Cell Biol 155:605-612(2001) [PubMed] [Europe PMC] [Abstract]
protein kinase C binding
Source: UniProtKB
Inferred from direct assayⁱ
- Ref.1
  "Cypher, a striated muscle-restricted PDZ and LIM domain-containing protein, binds to alpha-actinin-2 and protein kinase C."
  Zhou Q., Ruiz-Lozano P., Martone M.E., Chen J.
  J. Biol. Chem. 274:19807-19813(1999) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 6), INTERACTION WITH ACTN2 AND PKC, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Ligand	Metal-binding, Zinc

Ligand

Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: LIM domain-binding protein 3 Alternative name(s): Protein cypher Protein oracle Z-band alternatively spliced PDZ-motif protein
Gene namesⁱ	Name:Ldb3Imported Synonyms:Kiaa0613
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 14

Organism-specific databases

MGIⁱ	MGI:1344412, Ldb3

Keywords - Cellular componentⁱ

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000075768	1 – 723	LIM domain-binding protein 3Add BLAST		723

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	44	PhosphoserineCombined sources	1
Modified residueⁱ	98	PhosphoserineCombined sources	1
Modified residueⁱ	119	PhosphothreonineCombined sources	1
Modified residueⁱ	121	PhosphoserineCombined sources	1
Modified residueⁱ	123	PhosphoserineCombined sources	1
Modified residueⁱ	214	PhosphoserineCombined sources	1
Modified residueⁱ	216	Omega-N-methylarginineCombined sources	1
Modified residueⁱ	220	PhosphoserineCombined sources	1
Modified residueⁱ	251	PhosphoserineCombined sources	1
Modified residueⁱ	512	Omega-N-methylarginineCombined sources	1
Modified residueⁱ	529	Omega-N-methylarginineCombined sources	1
Isoform 21 Publication (identifier: Q9JKS4-2)
Modified residueⁱ	112	PhosphoserineCombined sources	1
Isoform 41 Publication (identifier: Q9JKS4-4)
Modified residueⁱ	112	PhosphoserineCombined sources	1
Modified residueⁱ	291	Omega-N-methylarginineCombined sources	1
Isoform 62 Publications (identifier: Q9JKS4-6)
Modified residueⁱ	112	PhosphoserineCombined sources	1
Modified residueⁱ	288	PhosphoserineCombined sources	1
Isoform 51 Publication (identifier: Q9JKS4-5)
Modified residueⁱ	327	PhosphoserineCombined sources	1
Isoform 32 Publications (identifier: Q9JKS4-3)
Modified residueⁱ	330	Omega-N-methylarginineCombined sources	1

Keywords - PTMⁱ

Methylation, Phosphoprotein

Proteomic databases

PaxDbⁱ	Q9JKS4
PeptideAtlas More...PeptideAtlasⁱ	Q9JKS4
PRIDEⁱ	Q9JKS4

PTM databases

iPTMnetⁱ	Q9JKS4
PhosphoSitePlusⁱ	Q9JKS4

Expressionⁱ

Tissue specificityⁱ

Expressed primarily in adult heart and skeletal muscle, and detected at lower levels in lung. Isoforms are expressed in a tissue-specific manner. Isoform 1, isoform 3 and isoform 5 are expressed in heart, whereas isoform 2, isoform 4 and isoform 6 are expressed in skeletal muscle.4 Publications

Developmental stageⁱ

Initially expressed in a myocardium-specific manner at 8.5-9 dpc and remains cardiac-restricted until day 12. Strongly expressed throughout heart in all stages examined. At 12.5 dpc expressed at low levels in non-cardiac striated muscles. By 14.5 dpc expressed at high levels in both cardiac and skeletal muscle, and also strongly expressed in striated muscles of tongue, thoracic and abdominal muscles, leg and diaphragm. The various isoforms are developmentally regulated in both skeletal and cardiac muscle. Isoform 5 and isoform 6, which are barely detectable during embryogenesis are up-regulated postnatally. In heart, isoform 3 is up-regulated developmentally, whereas the predominant isoform 1 is expressed throughout development and into adulthood. In skeletal muscle, the predominant isoform 2 is gradually replaced by isoform 4 postnatally.2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000021798, Expressed in gastrocnemius medialis and 284 other tissues
ExpressionAtlasⁱ	Q9JKS4, baseline and differential
Genevisibleⁱ	Q9JKS4, MM

Interactionⁱ

Subunit structureⁱ

Interacts via its LIM domains with various PKC isoforms.

Interacts via its PDZ domain with the ACTN2 C-terminal region.

Interacts with MYOZ1, MYOZ2 and MYOZ3.

By similarity1 Publication

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGRIDⁱ	204912, 17 interactors
IntActⁱ	Q9JKS4, 4 interactors
Molecular INTeraction database More...MINTⁱ	Q9JKS4
STRINGⁱ	10090.ENSMUSP00000022327

Miscellaneous databases

RNActⁱ	Q9JKS4, protein

RNActⁱ

Q9JKS4, protein

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	2 – 10	Combined sources	9
Beta strandⁱ	15 – 17	Combined sources	3
Turnⁱ	21 – 24	Combined sources	4
Beta strandⁱ	28 – 32	Combined sources	5
Helixⁱ	37 – 40	Combined sources	4
Beta strandⁱ	48 – 54	Combined sources	7
Beta strandⁱ	56 – 60	Combined sources	5
Helixⁱ	62 – 71	Combined sources	10
Beta strandⁱ	74 – 81	Combined sources	8

Show more details

3D structure databases

BMRBⁱ	Q9JKS4
SMRⁱ	Q9JKS4
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9JKS4

EvolutionaryTraceⁱ

Q9JKS4

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	1 – 84	PDZPROSITE-ProRule annotationAdd BLAST	84
Domainⁱ	545 – 603	LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST	59
Domainⁱ	604 – 663	LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST	60
Domainⁱ	664 – 723	LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST	60

Keywords - Domainⁱ

LIM domain, Repeat

Phylogenomic databases

eggNOGⁱ	KOG1703, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00940000154877
HOGENOMⁱ	CLU_038114_0_0_1
InParanoidⁱ	Q9JKS4
OMAⁱ	SNVYCER
Database of Orthologous Groups More...OrthoDBⁱ	1013114at2759
PhylomeDBⁱ	Q9JKS4
TreeFamⁱ	TF106408

Family and domain databases

Gene3Dⁱ	2.30.42.10, 1 hit
InterProⁱ	View protein in InterPro IPR031847, DUF4749 IPR001478, PDZ IPR036034, PDZ_sf IPR006643, Zasp-like_motif IPR001781, Znf_LIM
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF15936, DUF4749, 1 hit PF00412, LIM, 3 hits PF00595, PDZ, 1 hit
SMARTⁱ	View protein in SMART SM00132, LIM, 3 hits SM00228, PDZ, 1 hit SM00735, ZM, 1 hit
SUPFAMⁱ	SSF50156, SSF50156, 1 hit
PROSITEⁱ	View protein in PROSITE PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 3 hits PS50106, PDZ, 1 hit

Sequences (6+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 6 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 6 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform 11 Publication (identifier: Q9JKS4-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSYSVTLTGP GPWGFRLQGG KDFNMPLTIS RITPGSKAAQ SQLSQGDLVV 
        60         70         80         90        100
AIDGVNTDTM THLEAQNKIK SASYNLSLTL QKSKRPIPIS TTAPPIQSPL 
       110        120        130        140        150
PVIPHQKDPA LDTNGSLATP SPSPEARASP GALEFGDTFS SSFSQTSVCS 
       160        170        180        190        200
PLMEASGPVL PLGSPVAKAS SEGAQGSVSP KVLPGPSQPR QYNNPIGLYS 
       210        220        230        240        250
AETLREMAQM YQMSLRGKAS GAGLLGGSLP VKDLAVDSAS PVYQAVIKTQ 
       260        270        280        290        300
SKPEDEADEW ARRSSNLQSR SFRILAQMTG TEYMQDPDEE ALRRSSTPIE 
       310        320        330        340        350
HAPVCTSQAT SPLLPASAQS PAAASPIAAS PTLATAAATH AAAASAAGPA 
       360        370        380        390        400
ASPVENPRPQ ASAYSPAAAA SPAPSAHTSY SEGPAAPAPK PRVVTTASIR 
       410        420        430        440        450
PSVYQPVPAS SYSPSPGANY SPTPYTPSPA PAYTPSPAPT YTPSPAPTYS 
       460        470        480        490        500
PSPAPAYTPS PAPNYTPTPS AAYSGGPSES ASRPPWVTDD SFSQKFAPGK 
       510        520        530        540        550
STTTVSKQTL PRGAPAYNPT GPQVTPLARG TFQRAERFPA SSRTPLCGHC 
       560        570        580        590        600
NNVIRGPFLV AMGRSWHPEE FNCAYCKTSL ADVCFVEEQN NVYCERCYEQ 
       610        620        630        640        650
FFAPICAKCN TKIMGEVMHA LRQTWHTTCF VCAACKKPFG NSLFHMEDGE 
       660        670        680        690        700
PYCEKDYINL FSTKCHGCDF PVEAGDKFIE ALGHTWHDTC FICAVCHVNL 
       710        720 
EGQPFYSKKD KPLCKKHAHA INV

Length:723

Mass (Da):76,432

Last modified:October 1, 2000 - v1

Checksum:ⁱCC67D38AC2FFA6B6

GO

Isoform 21 Publication (identifier: Q9JKS4-2) [UniParc]FASTA Add to basket

Also known as: Cypher1s1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
108-227: DPALDTNGSL...KASGAGLLGG → VVANSPANAD...SDFSGASPLA

« Hide

        10         20         30         40         50
MSYSVTLTGP GPWGFRLQGG KDFNMPLTIS RITPGSKAAQ SQLSQGDLVV 
        60         70         80         90        100
AIDGVNTDTM THLEAQNKIK SASYNLSLTL QKSKRPIPIS TTAPPIQSPL 
       110        120        130        140        150
PVIPHQKVVA NSPANADYQE RFNPSVLKDS ALSTHKPIEV KGLGGKATII 
       160        170        180        190        200
HAQYNTPISM YSQDAIMDAI AGQAQAQGSD FSGASPLASL PVKDLAVDSA 
       210        220        230        240        250
SPVYQAVIKT QSKPEDEADE WARRSSNLQS RSFRILAQMT GTEYMQDPDE 
       260        270        280        290        300
EALRRSSTPI EHAPVCTSQA TSPLLPASAQ SPAAASPIAA SPTLATAAAT 
       310        320        330        340        350
HAAAASAAGP AASPVENPRP QASAYSPAAA ASPAPSAHTS YSEGPAAPAP 
       360        370        380        390        400
KPRVVTTASI RPSVYQPVPA SSYSPSPGAN YSPTPYTPSP APAYTPSPAP 
       410        420        430        440        450
TYTPSPAPTY SPSPAPAYTP SPAPNYTPTP SAAYSGGPSE SASRPPWVTD 
       460        470        480        490        500
DSFSQKFAPG KSTTTVSKQT LPRGAPAYNP TGPQVTPLAR GTFQRAERFP 
       510        520        530        540        550
ASSRTPLCGH CNNVIRGPFL VAMGRSWHPE EFNCAYCKTS LADVCFVEEQ 
       560        570        580        590        600
NNVYCERCYE QFFAPICAKC NTKIMGEVMH ALRQTWHTTC FVCAACKKPF 
       610        620        630        640        650
GNSLFHMEDG EPYCEKDYIN LFSTKCHGCD FPVEAGDKFI EALGHTWHDT 
       660        670        680 
CFICAVCHVN LEGQPFYSKK DKPLCKKHAH AINV

Show »

Length:684

Mass (Da):72,752

Checksum:ⁱB8969D96ECC7C7C5

GO

Isoform 32 Publications (identifier: Q9JKS4-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
296-357: Missing.

« Hide

        10         20         30         40         50
MSYSVTLTGP GPWGFRLQGG KDFNMPLTIS RITPGSKAAQ SQLSQGDLVV 
        60         70         80         90        100
AIDGVNTDTM THLEAQNKIK SASYNLSLTL QKSKRPIPIS TTAPPIQSPL 
       110        120        130        140        150
PVIPHQKDPA LDTNGSLATP SPSPEARASP GALEFGDTFS SSFSQTSVCS 
       160        170        180        190        200
PLMEASGPVL PLGSPVAKAS SEGAQGSVSP KVLPGPSQPR QYNNPIGLYS 
       210        220        230        240        250
AETLREMAQM YQMSLRGKAS GAGLLGGSLP VKDLAVDSAS PVYQAVIKTQ 
       260        270        280        290        300
SKPEDEADEW ARRSSNLQSR SFRILAQMTG TEYMQDPDEE ALRRSRPQAS 
       310        320        330        340        350
AYSPAAAASP APSAHTSYSE GPAAPAPKPR VVTTASIRPS VYQPVPASSY 
       360        370        380        390        400
SPSPGANYSP TPYTPSPAPA YTPSPAPTYT PSPAPTYSPS PAPAYTPSPA 
       410        420        430        440        450
PNYTPTPSAA YSGGPSESAS RPPWVTDDSF SQKFAPGKST TTVSKQTLPR 
       460        470        480        490        500
GAPAYNPTGP QVTPLARGTF QRAERFPASS RTPLCGHCNN VIRGPFLVAM 
       510        520        530        540        550
GRSWHPEEFN CAYCKTSLAD VCFVEEQNNV YCERCYEQFF APICAKCNTK 
       560        570        580        590        600
IMGEVMHALR QTWHTTCFVC AACKKPFGNS LFHMEDGEPY CEKDYINLFS 
       610        620        630        640        650
TKCHGCDFPV EAGDKFIEAL GHTWHDTCFI CAVCHVNLEG QPFYSKKDKP 
       660 
LCKKHAHAIN V

Show »

Length:661

Mass (Da):70,751

Checksum:ⁱ5DEFEEE78D45532F

GO

Isoform 41 Publication (identifier: Q9JKS4-4) [UniParc]FASTA Add to basket

Also known as: Cypher3s1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
108-227: DPALDTNGSL...KASGAGLLGG → VVANSPANAD...SDFSGASPLA
296-357: Missing.

« Hide

        10         20         30         40         50
MSYSVTLTGP GPWGFRLQGG KDFNMPLTIS RITPGSKAAQ SQLSQGDLVV 
        60         70         80         90        100
AIDGVNTDTM THLEAQNKIK SASYNLSLTL QKSKRPIPIS TTAPPIQSPL 
       110        120        130        140        150
PVIPHQKVVA NSPANADYQE RFNPSVLKDS ALSTHKPIEV KGLGGKATII 
       160        170        180        190        200
HAQYNTPISM YSQDAIMDAI AGQAQAQGSD FSGASPLASL PVKDLAVDSA 
       210        220        230        240        250
SPVYQAVIKT QSKPEDEADE WARRSSNLQS RSFRILAQMT GTEYMQDPDE 
       260        270        280        290        300
EALRRSRPQA SAYSPAAAAS PAPSAHTSYS EGPAAPAPKP RVVTTASIRP 
       310        320        330        340        350
SVYQPVPASS YSPSPGANYS PTPYTPSPAP AYTPSPAPTY TPSPAPTYSP 
       360        370        380        390        400
SPAPAYTPSP APNYTPTPSA AYSGGPSESA SRPPWVTDDS FSQKFAPGKS 
       410        420        430        440        450
TTTVSKQTLP RGAPAYNPTG PQVTPLARGT FQRAERFPAS SRTPLCGHCN 
       460        470        480        490        500
NVIRGPFLVA MGRSWHPEEF NCAYCKTSLA DVCFVEEQNN VYCERCYEQF 
       510        520        530        540        550
FAPICAKCNT KIMGEVMHAL RQTWHTTCFV CAACKKPFGN SLFHMEDGEP 
       560        570        580        590        600
YCEKDYINLF STKCHGCDFP VEAGDKFIEA LGHTWHDTCF ICAVCHVNLE 
       610        620 
GQPFYSKKDK PLCKKHAHAI NV

Show »

Length:622

Mass (Da):67,071

Checksum:ⁱABC92BB80C3B9903

GO

Isoform 51 Publication (identifier: Q9JKS4-5) [UniParc]FASTA Add to basket

Also known as: Cypher2c1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
296-327: STPIEHAPVCTSQATSPLLPASAQSPAAASPI → RERFETERNSPRFAKLRNWHHGLSAQILNVKS
328-723: Missing.

« Hide

        10         20         30         40         50
MSYSVTLTGP GPWGFRLQGG KDFNMPLTIS RITPGSKAAQ SQLSQGDLVV 
        60         70         80         90        100
AIDGVNTDTM THLEAQNKIK SASYNLSLTL QKSKRPIPIS TTAPPIQSPL 
       110        120        130        140        150
PVIPHQKDPA LDTNGSLATP SPSPEARASP GALEFGDTFS SSFSQTSVCS 
       160        170        180        190        200
PLMEASGPVL PLGSPVAKAS SEGAQGSVSP KVLPGPSQPR QYNNPIGLYS 
       210        220        230        240        250
AETLREMAQM YQMSLRGKAS GAGLLGGSLP VKDLAVDSAS PVYQAVIKTQ 
       260        270        280        290        300
SKPEDEADEW ARRSSNLQSR SFRILAQMTG TEYMQDPDEE ALRRSRERFE 
       310        320 
TERNSPRFAK LRNWHHGLSA QILNVKS

Show »

Length:327

Mass (Da):35,108

Checksum:ⁱ603BEF5D6152B848

GO

Isoform 62 Publications (identifier: Q9JKS4-6) [UniParc]FASTA Add to basket

Also known as: Cypher2s1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     108-227: DPALDTNGSL...KASGAGLLGG → VVANSPANAD...SDFSGASPLA
     296-327: STPIEHAPVCTSQATSPLLPASAQSPAAASPI → RERFETERNSPRFAKLRNWHHGLSAQILNVKS
     328-723: Missing.

« Hide

        10         20         30         40         50
MSYSVTLTGP GPWGFRLQGG KDFNMPLTIS RITPGSKAAQ SQLSQGDLVV 
        60         70         80         90        100
AIDGVNTDTM THLEAQNKIK SASYNLSLTL QKSKRPIPIS TTAPPIQSPL 
       110        120        130        140        150
PVIPHQKVVA NSPANADYQE RFNPSVLKDS ALSTHKPIEV KGLGGKATII 
       160        170        180        190        200
HAQYNTPISM YSQDAIMDAI AGQAQAQGSD FSGASPLASL PVKDLAVDSA 
       210        220        230        240        250
SPVYQAVIKT QSKPEDEADE WARRSSNLQS RSFRILAQMT GTEYMQDPDE 
       260        270        280 
EALRRSRERF ETERNSPRFA KLRNWHHGLS AQILNVKS

Show »

Length:288

Mass (Da):31,427

Checksum:ⁱ65B11275409291B6

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	E9PYJ9	E9PYJ9_MOUSE		LIM domain-binding protein 3 LIM domain-binding protein 3	Ldb3	679	Annotation score:

Sequence cautionⁱ

The sequence BAB23128 differs from that shown. Sequencing errors.Curated

The sequence BAD32258 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	108 – 145	DPALD…SSFSQ → VVANSPANADYQERFNPSVL KGLSSVLKGLSSVHPQAH in BAB23128 (PubMed:16141072).CuratedAdd BLAST	38
Sequence conflictⁱ	450	S → T in AAD42950 (PubMed:10391924).Curated	1
Sequence conflictⁱ	450	S → T in AAO26188 (PubMed:12499364).Curated	1
Sequence conflictⁱ	450	S → T in AAO26189 (PubMed:12499364).Curated	1
Sequence conflictⁱ	512	R → W in BAD32258 (PubMed:15368895).Curated	1
Isoform 21 Publication (identifier: Q9JKS4-2)
Sequence conflictⁱ	176 – 188	AQGSD…ASPLA → AQGSDFSG in AAO26189 (PubMed:12499364).CuratedAdd BLAST	13

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_051903	108 – 227	DPALD…GLLGG → VVANSPANADYQERFNPSVL KDSALSTHKPIEVKGLGGKA TIIHAQYNTPISMYSQDAIM DAIAGQAQAQGSDFSGASPL A in isoform 2, isoform 4 and isoform 6. 5 PublicationsAdd BLAST	120
Alternative sequenceⁱVSP_051904	296 – 357	Missing in isoform 3 and isoform 4. 3 PublicationsAdd BLAST	62
Alternative sequenceⁱVSP_051905	296 – 327	STPIE…AASPI → RERFETERNSPRFAKLRNWH HGLSAQILNVKS in isoform 5 and isoform 6. 5 PublicationsAdd BLAST	32
Alternative sequenceⁱVSP_051906	328 – 723	Missing in isoform 5 and isoform 6. 5 PublicationsAdd BLAST	396

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF114378 mRNA Translation: AAD42950.2 AF114379 mRNA Translation: AAD42951.2 AJ005621 mRNA Translation: CAB46747.1 AF228057 mRNA Translation: AAF33847.1 AF228058 mRNA Translation: AAF33848.1 AY206011 mRNA Translation: AAO26187.1 AY206012 mRNA Translation: AAO26188.1 AY206013 mRNA Translation: AAO26189.1 AY206015 mRNA Translation: AAO26190.1 AK172980 mRNA Translation: BAD32258.1 Different initiation. AK004020 mRNA Translation: BAB23128.1 Sequence problems. AK137181 mRNA Translation: BAE23262.1 AK142292 mRNA Translation: BAE25016.1 BC099596 mRNA Translation: AAH99596.1 BC138793 mRNA Translation: AAI38794.1 BC145420 mRNA Translation: AAI45421.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS26940.1 [Q9JKS4-3] CCDS26941.1 [Q9JKS4-1] CCDS26942.1 [Q9JKS4-5] CCDS36879.1 [Q9JKS4-2] CCDS88626.1 [Q9JKS4-4] CCDS88627.1 [Q9JKS4-6]
NCBI Reference Sequences More...RefSeqⁱ	NP_001034160.1, NM_001039071.2 [Q9JKS4-3] NP_001034161.1, NM_001039072.2 NP_001034162.1, NM_001039073.2 [Q9JKS4-4] NP_001034163.1, NM_001039074.2 [Q9JKS4-2] NP_001034164.1, NM_001039075.2 [Q9JKS4-6] NP_001034165.1, NM_001039076.2 [Q9JKS4-5] NP_036048.3, NM_011918.4 [Q9JKS4-1] XP_006519080.1, XM_006519017.3 [Q9JKS4-2] XP_017171508.1, XM_017316019.1 [Q9JKS4-1]

Genome annotation databases

Ensemblⁱ	ENSMUST00000022327; ENSMUSP00000022327; ENSMUSG00000021798 [Q9JKS4-1] ENSMUST00000022328; ENSMUSP00000022328; ENSMUSG00000021798 [Q9JKS4-3] ENSMUST00000022330; ENSMUSP00000022330; ENSMUSG00000021798 [Q9JKS4-5] ENSMUST00000090040; ENSMUSP00000087494; ENSMUSG00000021798 [Q9JKS4-2] ENSMUST00000227819; ENSMUSP00000154119; ENSMUSG00000021798 [Q9JKS4-6] ENSMUST00000228044; ENSMUSP00000154758; ENSMUSG00000021798 [Q9JKS4-4]
GeneIDⁱ	24131
KEGGⁱ	mmu:24131
UCSC genome browser More...UCSCⁱ	uc007taz.1, mouse [Q9JKS4-2] uc007tba.1, mouse [Q9JKS4-4] uc007tbc.1, mouse [Q9JKS4-1] uc007tbd.1, mouse [Q9JKS4-3] uc007tbe.1, mouse [Q9JKS4-6] uc007tbf.1, mouse [Q9JKS4-5]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9JKS4-6	LIM domain-binding 3		RAT		288	UniRef100_A0A0G2JXR0

Protein	Similar proteins		Species	Score	Length	Source
Q9JKS4	LIM domain-binding protein 3 isoform X1		MUSCR		723	UniRef90_Q9JKS4
Isoform 2 of LIM domain-binding protein 3		MOUSE		684
Q9JKS4-3	LIM domain binding 3		SURSU		784	UniRef90_Q9JKS4-3
LIM domain-binding 3		RAT		726
Uncharacterized protein		ICTTR		725
LIM domain-binding protein 3 isoform X5		MESAU		684
LIM domain-binding protein 3		MOUSE		679
+48
Q9JKS4-4	LIM domain-binding protein 3 isoform X5		CARSF		631	UniRef90_Q9JKS4-4
LIM domain binding 3		CALJA		622
LIM domain-binding protein 3 isoform X1		MESAU		622
LIM domain-binding protein 3 isoform X1		PERMB		622
LIM domain binding 3		GORGO		617
+63
Q9JKS4-5	LIM domain-binding protein 3 isoform X11		SHEEP		371	UniRef90_Q9JKS4-5
LIM domain-binding protein 3 isoform X6		ERIEU		330
LIM domain binding 3		BOVIN		330
LIM domain-binding protein 3 isoform X7		TURTR		330
LIM domain-binding protein 3 isoform X6		Ursus arctos horribilis		330
+19
Q9JKS4-6	LIM domain-binding 3		RAT		288	UniRef90_A0A0G2JXR0
PDZ domain-containing protein		MONDO		303
LIM domain-binding protein 3		CASCN		288
LIM domain-binding protein 3 isoform X8		OCTDE		288
LIM domain-binding protein 3 isoform X3		PERMB		288
+31

Protein	Similar proteins		Species	Score	Length	Source
Q9JKS4	LIM domain-binding protein 3 isoform X1		MUSCR		723	UniRef50_Q9JKS4
Isoform 2 of LIM domain-binding protein 3		MOUSE		684
Uncharacterized protein		PROCO		698
Uncharacterized protein		PANPA		687
LDB3 isoform 1		PONAB		723
+176
Q9JKS4-3	LIM domain binding 3		SURSU		784	UniRef50_Q9JKS4-3
LIM domain-binding 3		RAT		726
Uncharacterized protein		ICTTR		725
LIM domain-binding protein 3 isoform X5		MESAU		684
LIM domain-binding protein 3		MOUSE		679
+117
Q9JKS4-5	LIM domain-binding protein 3 isoform X11		SHEEP		371	UniRef50_Q9JKS4-5
LIM domain binding 3		BOVIN		330
LIM domain-binding protein 3 isoform X7		TURTR		330
LIM domain-binding protein 3 isoform X6		ERIEU		330
LIM domain-binding protein 3 isoform X6		Ursus arctos horribilis		330
+21
Q9JKS4-6	Cypher/ZASP splice variant 2 gamma		DANRE		291	UniRef50_Q4ZGK9
PDZ domain-containing protein		Sinocyclocheilus anshuiensis		291
PDZ domain-containing protein		SINGR		291
LIM domain-binding protein 3-like isoform X5		CARAU		291
PDZ domain-containing protein		MONDO		303
+147

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF114378 mRNA Translation: AAD42950.2 AF114379 mRNA Translation: AAD42951.2 AJ005621 mRNA Translation: CAB46747.1 AF228057 mRNA Translation: AAF33847.1 AF228058 mRNA Translation: AAF33848.1 AY206011 mRNA Translation: AAO26187.1 AY206012 mRNA Translation: AAO26188.1 AY206013 mRNA Translation: AAO26189.1 AY206015 mRNA Translation: AAO26190.1 AK172980 mRNA Translation: BAD32258.1 Different initiation. AK004020 mRNA Translation: BAB23128.1 Sequence problems. AK137181 mRNA Translation: BAE23262.1 AK142292 mRNA Translation: BAE25016.1 BC099596 mRNA Translation: AAH99596.1 BC138793 mRNA Translation: AAI38794.1 BC145420 mRNA Translation: AAI45421.1
CCDSⁱ	CCDS26940.1 [Q9JKS4-3] CCDS26941.1 [Q9JKS4-1] CCDS26942.1 [Q9JKS4-5] CCDS36879.1 [Q9JKS4-2] CCDS88626.1 [Q9JKS4-4] CCDS88627.1 [Q9JKS4-6]
RefSeqⁱ	NP_001034160.1, NM_001039071.2 [Q9JKS4-3] NP_001034161.1, NM_001039072.2 NP_001034162.1, NM_001039073.2 [Q9JKS4-4] NP_001034163.1, NM_001039074.2 [Q9JKS4-2] NP_001034164.1, NM_001039075.2 [Q9JKS4-6] NP_001034165.1, NM_001039076.2 [Q9JKS4-5] NP_036048.3, NM_011918.4 [Q9JKS4-1] XP_006519080.1, XM_006519017.3 [Q9JKS4-2] XP_017171508.1, XM_017316019.1 [Q9JKS4-1]

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1WJL	NMR	-	A	1-83	[»]
BMRBⁱ	Q9JKS4
SMRⁱ	Q9JKS4
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	204912, 17 interactors
IntActⁱ	Q9JKS4, 4 interactors
MINTⁱ	Q9JKS4
STRINGⁱ	10090.ENSMUSP00000022327

PTM databases

iPTMnetⁱ	Q9JKS4
PhosphoSitePlusⁱ	Q9JKS4

Proteomic databases

PaxDbⁱ	Q9JKS4
PeptideAtlasⁱ	Q9JKS4
PRIDEⁱ	Q9JKS4

Protocols and materials databases

Antibodypediaⁱ	15974, 246 antibodies

Antibodypediaⁱ

15974, 246 antibodies

Genome annotation databases

Ensemblⁱ	ENSMUST00000022327; ENSMUSP00000022327; ENSMUSG00000021798 [Q9JKS4-1] ENSMUST00000022328; ENSMUSP00000022328; ENSMUSG00000021798 [Q9JKS4-3] ENSMUST00000022330; ENSMUSP00000022330; ENSMUSG00000021798 [Q9JKS4-5] ENSMUST00000090040; ENSMUSP00000087494; ENSMUSG00000021798 [Q9JKS4-2] ENSMUST00000227819; ENSMUSP00000154119; ENSMUSG00000021798 [Q9JKS4-6] ENSMUST00000228044; ENSMUSP00000154758; ENSMUSG00000021798 [Q9JKS4-4]
GeneIDⁱ	24131
KEGGⁱ	mmu:24131
UCSCⁱ	uc007taz.1, mouse [Q9JKS4-2] uc007tba.1, mouse [Q9JKS4-4] uc007tbc.1, mouse [Q9JKS4-1] uc007tbd.1, mouse [Q9JKS4-3] uc007tbe.1, mouse [Q9JKS4-6] uc007tbf.1, mouse [Q9JKS4-5]

Organism-specific databases

CTDⁱ	11155
MGIⁱ	MGI:1344412, Ldb3
Rougeⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG1703, Eukaryota
GeneTreeⁱ	ENSGT00940000154877
HOGENOMⁱ	CLU_038114_0_0_1
InParanoidⁱ	Q9JKS4
OMAⁱ	SNVYCER
OrthoDBⁱ	1013114at2759
PhylomeDBⁱ	Q9JKS4
TreeFamⁱ	TF106408

Miscellaneous databases

BioGRID-ORCSⁱ	24131, 0 hits in 17 CRISPR screens
ChiTaRSⁱ	Ldb3, mouse
EvolutionaryTraceⁱ	Q9JKS4
Protein Ontology More...PROⁱ	PR:Q9JKS4
RNActⁱ	Q9JKS4, protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000021798, Expressed in gastrocnemius medialis and 284 other tissues
ExpressionAtlasⁱ	Q9JKS4, baseline and differential
Genevisibleⁱ	Q9JKS4, MM

Family and domain databases

Gene3Dⁱ	2.30.42.10, 1 hit
InterProⁱ	View protein in InterPro IPR031847, DUF4749 IPR001478, PDZ IPR036034, PDZ_sf IPR006643, Zasp-like_motif IPR001781, Znf_LIM
Pfamⁱ	View protein in Pfam PF15936, DUF4749, 1 hit PF00412, LIM, 3 hits PF00595, PDZ, 1 hit
SMARTⁱ	View protein in SMART SM00132, LIM, 3 hits SM00228, PDZ, 1 hit SM00735, ZM, 1 hit
SUPFAMⁱ	SSF50156, SSF50156, 1 hit
PROSITEⁱ	View protein in PROSITE PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 3 hits PS50106, PDZ, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	LDB3_MOUSE
Accessionⁱ	Q9JKS4Primary (citable) accession number: Q9JKS4 Secondary accession number(s): B2RSB0 Q9WVH2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
	Last sequence update:	October 1, 2000
	Last modified:	December 2, 2020
	This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9JKS4 (LDB3_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

LIM domain-binding protein 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytoskeleton

Other locations

Cytoskeleton

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ