UniProtKB - Q9JKN6 (NOVA1_MOUSE)
Protein
RNA-binding protein Nova-1
Gene
Nova1
Organism
Mus musculus (Mouse)
Status
Functioni
Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion. It binds specifically to the sequence UCAUY. Most likely acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer.3 Publications
GO - Molecular functioni
- mRNA 3'-UTR binding Source: MGI
- mRNA binding Source: MGI
- RNA binding Source: MGI
GO - Biological processi
- mRNA splicing, via spliceosome Source: MGI
- negative regulation of cold-induced thermogenesis Source: YuBioLab
- regulation of alternative mRNA splicing, via spliceosome Source: GO_Central
- regulation of gene expression Source: GO_Central
- regulation of mRNA processing Source: MGI
- regulation of RNA metabolic process Source: MGI
Keywordsi
Molecular function | RNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: RNA-binding protein Nova-1Alternative name(s): Neuro-oncological ventral antigen 1 Ventral neuron-specific protein 1 |
Gene namesi | Name:Nova1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104297, Nova1 |
Subcellular locationi
Nucleus
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Involvement in diseasei
Defects in Nova1 leads to neuronal death in spinal and brainstem neurons.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050117 | 1 – 507 | RNA-binding protein Nova-1Add BLAST | 507 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 154 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q9JKN6 |
PaxDbi | Q9JKN6 |
PeptideAtlasi | Q9JKN6 |
PRIDEi | Q9JKN6 |
PTM databases
iPTMneti | Q9JKN6 |
PhosphoSitePlusi | Q9JKN6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000021047, Expressed in midbrain and 229 other tissues |
ExpressionAtlasi | Q9JKN6, baseline and differential |
Genevisiblei | Q9JKN6, MM |
Interactioni
Subunit structurei
Interacts with PTBP2; the interaction is direct.
1 PublicationProtein-protein interaction databases
BioGRIDi | 576757, 3 interactors |
IntActi | Q9JKN6, 2 interactors |
MINTi | Q9JKN6 |
STRINGi | 10090.ENSMUSP00000021438 |
Miscellaneous databases
RNActi | Q9JKN6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 49 – 116 | KH 1PROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 171 – 237 | KH 2PROSITE-ProRule annotationAdd BLAST | 67 | |
Domaini | 421 – 488 | KH 3PROSITE-ProRule annotationAdd BLAST | 68 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 27 – 43 | Bipartite nuclear localization signalSequence analysisAdd BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 273 – 409 | Ala-richAdd BLAST | 137 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2191, Eukaryota |
GeneTreei | ENSGT00940000155573 |
HOGENOMi | CLU_022670_1_1_1 |
InParanoidi | Q9JKN6 |
OMAi | VVDFIMD |
OrthoDBi | 1167935at2759 |
PhylomeDBi | Q9JKN6 |
TreeFami | TF316981 |
Family and domain databases
Gene3Di | 3.30.1370.10, 3 hits |
InterProi | View protein in InterPro IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR033086, NOVA1 |
PANTHERi | PTHR10288:SF229, PTHR10288:SF229, 1 hit |
Pfami | View protein in Pfam PF00013, KH_1, 3 hits |
SMARTi | View protein in SMART SM00322, KH, 3 hits |
SUPFAMi | SSF54791, SSF54791, 3 hits |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9JKN6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY
60 70 80 90 100
FLKVLIPSYA AGSIIGKGGQ TIVQLQKETG ATIKLSKSKD FYPGTTERVC
110 120 130 140 150
LIQGTIEALN AVHGFIAEKI REMPQNVAKT EPVSILQPQT TVNPDRIKQT
160 170 180 190 200
LPSSPTTTKS SPSDPMTTSR ANQVKIIVPN STAGLIIGKG GATVKAIMEQ
210 220 230 240 250
SGAWVQLSQK PDGINLQERV VTVSGEPEQN RKAVELIIQK IQEDPQSGSC
260 270 280 290 300
LNISYANVTG PVANSNPTGS PYANTAEVLP TAAAAAGLLG HANLAGVAAF
310 320 330 340 350
PAVLSGFTGN DLVAITSALN TLASYGYNLN TLGLGLSQAA ATGALAAAAA
360 370 380 390 400
SANPAAAAAN LLATYASEAS ASGSTAGGTA GTFALGSLAA ATAATNGYFG
410 420 430 440 450
AASPLAASAI LGTEKSTDGS KDVVEIAVPE NLVGAILGKG GKTLVEYQEL
460 470 480 490 500
TGARIQISKK GEFVPGTRNR KVTITGTPAA TQAAQYLITQ RITYEQGVRA
ANPQKVG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P6P4 | A0A1W2P6P4_MOUSE | RNA-binding protein Nova-1 | Nova1 | 112 | Annotation score: | ||
A0A1W2P6S8 | A0A1W2P6S8_MOUSE | RNA-binding protein Nova-1 | Nova1 | 66 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC108802 Genomic DNA No translation available. AC156636 Genomic DNA No translation available. AF232828 mRNA Translation: AAF35907.1 AK047565 mRNA Translation: BAC33088.1 |
CCDSi | CCDS49058.1 |
RefSeqi | NP_067336.1, NM_021361.1 |
Genome annotation databases
Ensembli | ENSMUST00000021438; ENSMUSP00000021438; ENSMUSG00000021047 |
GeneIDi | 664883 |
KEGGi | mmu:664883 |
UCSCi | uc007nmd.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC108802 Genomic DNA No translation available. AC156636 Genomic DNA No translation available. AF232828 mRNA Translation: AAF35907.1 AK047565 mRNA Translation: BAC33088.1 |
CCDSi | CCDS49058.1 |
RefSeqi | NP_067336.1, NM_021361.1 |
3D structure databases
SMRi | Q9JKN6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 576757, 3 interactors |
IntActi | Q9JKN6, 2 interactors |
MINTi | Q9JKN6 |
STRINGi | 10090.ENSMUSP00000021438 |
PTM databases
iPTMneti | Q9JKN6 |
PhosphoSitePlusi | Q9JKN6 |
Proteomic databases
MaxQBi | Q9JKN6 |
PaxDbi | Q9JKN6 |
PeptideAtlasi | Q9JKN6 |
PRIDEi | Q9JKN6 |
Protocols and materials databases
Antibodypediai | 133, 249 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000021438; ENSMUSP00000021438; ENSMUSG00000021047 |
GeneIDi | 664883 |
KEGGi | mmu:664883 |
UCSCi | uc007nmd.2, mouse |
Organism-specific databases
CTDi | 4857 |
MGIi | MGI:104297, Nova1 |
Phylogenomic databases
eggNOGi | KOG2191, Eukaryota |
GeneTreei | ENSGT00940000155573 |
HOGENOMi | CLU_022670_1_1_1 |
InParanoidi | Q9JKN6 |
OMAi | VVDFIMD |
OrthoDBi | 1167935at2759 |
PhylomeDBi | Q9JKN6 |
TreeFami | TF316981 |
Miscellaneous databases
BioGRID-ORCSi | 664883, 0 hits in 17 CRISPR screens |
ChiTaRSi | Nova1, mouse |
PROi | PR:Q9JKN6 |
RNActi | Q9JKN6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021047, Expressed in midbrain and 229 other tissues |
ExpressionAtlasi | Q9JKN6, baseline and differential |
Genevisiblei | Q9JKN6, MM |
Family and domain databases
Gene3Di | 3.30.1370.10, 3 hits |
InterProi | View protein in InterPro IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR033086, NOVA1 |
PANTHERi | PTHR10288:SF229, PTHR10288:SF229, 1 hit |
Pfami | View protein in Pfam PF00013, KH_1, 3 hits |
SMARTi | View protein in SMART SM00322, KH, 3 hits |
SUPFAMi | SSF54791, SSF54791, 3 hits |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NOVA1_MOUSE | |
Accessioni | Q9JKN6Primary (citable) accession number: Q9JKN6 Secondary accession number(s): Q8C8B9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 4, 2003 |
Last sequence update: | January 9, 2007 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot