UniProtKB - Q9JKM5 (S1PR5_RAT)
Protein
Sphingosine 1-phosphate receptor 5
Gene
S1pr5
Organism
Rattus norvegicus (Rat)
Status
Functioni
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/O)alpha and G(12) subclass of heteromeric G-proteins. Displays antiproliferative effects in transfected CHO-K1 and HEK293 cells.
GO - Molecular functioni
- G protein-coupled receptor activity Source: RGD
- sphingosine-1-phosphate receptor activity Source: InterPro
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: GO_Central
- regulation of metabolic process Source: GO_Central
- regulation of neuron differentiation Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-418594, G alpha (i) signalling events R-RNO-419408, Lysosphingolipid and LPA receptors |
Names & Taxonomyi
Protein namesi | Recommended name: Sphingosine 1-phosphate receptor 5Short name: S1P receptor 5 Short name: S1P5 Alternative name(s): Endothelial differentiation G-protein-coupled receptor 8 Nerve growth factor-regulated G-protein-coupled receptor 1 Short name: NRG-1 Sphingosine 1-phosphate receptor Edg-8 Short name: S1P receptor Edg-8 |
Gene namesi | Name:S1pr5 Synonyms:Edg8, Nrg1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620566, S1pr5 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- integral component of membrane Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 40 | ExtracellularBy similarityAdd BLAST | 40 | |
Transmembranei | 41 – 61 | Helical; Name=1By similarityAdd BLAST | 21 | |
Topological domaini | 62 – 69 | CytoplasmicBy similarity | 8 | |
Transmembranei | 70 – 90 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 91 – 111 | ExtracellularBy similarityAdd BLAST | 21 | |
Transmembranei | 112 – 132 | Helical; Name=3By similarityAdd BLAST | 21 | |
Topological domaini | 133 – 151 | CytoplasmicBy similarityAdd BLAST | 19 | |
Transmembranei | 152 – 172 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 173 – 192 | ExtracellularBy similarityAdd BLAST | 20 | |
Transmembranei | 193 – 213 | Helical; Name=5By similarityAdd BLAST | 21 | |
Topological domaini | 214 – 253 | CytoplasmicBy similarityAdd BLAST | 40 | |
Transmembranei | 254 – 274 | Helical; Name=6By similarityAdd BLAST | 21 | |
Topological domaini | 275 – 288 | ExtracellularBy similarityAdd BLAST | 14 | |
Transmembranei | 289 – 309 | Helical; Name=7By similarityAdd BLAST | 21 | |
Topological domaini | 310 – 400 | CytoplasmicBy similarityAdd BLAST | 91 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069439 | 1 – 400 | Sphingosine 1-phosphate receptor 5Add BLAST | 400 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 20 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 324 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 340 | PhosphoserineCombined sources | 1 | |
Modified residuei | 342 | PhosphoserineCombined sources | 1 | |
Modified residuei | 384 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | Q9JKM5 |
PRIDEi | Q9JKM5 |
PTM databases
GlyGeni | Q9JKM5, 1 site |
iPTMneti | Q9JKM5 |
PhosphoSitePlusi | Q9JKM5 |
Expressioni
Tissue specificityi
Found almost exclusively in brain and skin. Ubiquitously detected in different brain regions with very prominent expression in lower brain regions such as the midbrain, pons, medulla, and spinal cord. Expressed predominantly within white matter tracts of the brain. Corpus callosum, optic nerve, olfactory tract, anterior commissure, internal and external capsules, fimbra of the hippocampus, mammillary tract, stria medullaris, and white matter of the cerebellum and brain stem all express the receptor. Within the striatum itself white matter fascicles express the receptor. Detected also in spleen and lung. Not observed in heart, liver, skeletal muscle, kidney, or testes.3 Publications
Inductioni
In PC-12 cells, nerve growth factor induces neuronal differentiation and represses expression of the receptor. Down-regulated also by fibroblast growth factor and dibutyryl cAMP. Epidermal growth factor, an agent that does not induce differentiation, does not repress expression. Protein kinase A appears to be an obligatory cellular component in regulation.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000020901, Expressed in brain and 22 other tissues |
Genevisiblei | Q9JKM5, RN |
Interactioni
Protein-protein interaction databases
BioGRIDi | 248819, 1 interactor |
STRINGi | 10116.ENSRNOP00000028380 |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QSWN, Eukaryota |
GeneTreei | ENSGT00970000193365 |
HOGENOMi | CLU_047979_1_0_1 |
InParanoidi | Q9JKM5 |
OMAi | MVTMRPY |
OrthoDBi | 981486at2759 |
PhylomeDBi | Q9JKM5 |
TreeFami | TF330052 |
Family and domain databases
InterProi | View protein in InterPro IPR005386, EDG8_S1P_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR004061, S1P_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01561, EDG8RECEPTOR PR00237, GPCRRHODOPSN PR01523, S1PRECEPTOR |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9JKM5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESGLLRPAP VSEVIVLHYN YTGKLRGARY QPGAGLRADA AVCLAVCAFI
60 70 80 90 100
VLENLAVLLV LGRHPRFHAP MFLLLGSLTL SDLLAGAAYA TNILLSGPLT
110 120 130 140 150
LRLSPALWFA REGGVFVALA ASVLSLLAIA LERHLTMARR GPAPAASRAR
160 170 180 190 200
TLAMAVAAWG LSLLLGLLPA LGWNCLGRLE ACSTVLPLYA KAYVLFCVLA
210 220 230 240 250
FLGILAAICA LYARIYCQVR ANARRLRAGP GSRRATSSSR SRHTPRSLAL
260 270 280 290 300
LRTLSVVLLA FVACWGPLFL LLLLDVACPA RACPVLLQAD PFLGLAMANS
310 320 330 340 350
LLNPIIYTFT NRDLRHALLR LLCCGRGPCN QDSSNSLQRS PSAVGPSGGG
360 370 380 390 400
LRRCLPPTLD RSSSPSEHSC PQRDGMDTSC STGSPGAATA NRTLVPDATD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 131 | L → I in AAF15395 (PubMed:10532805).Curated | 1 | |
Sequence conflicti | 162 – 164 | SLL → LLT in AAF15395 (PubMed:10532805).Curated | 3 | |
Sequence conflicti | 188 | L → V in AAF15395 (PubMed:10532805).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF115249 mRNA Translation: AAF15395.1 AF233649 mRNA Translation: AAF35912.1 |
RefSeqi | NP_068543.2, NM_021775.2 XP_006242725.1, XM_006242663.3 XP_006242726.1, XM_006242664.3 XP_006242727.1, XM_006242665.3 |
Genome annotation databases
Ensembli | ENSRNOT00000028380; ENSRNOP00000028380; ENSRNOG00000020901 |
GeneIDi | 60399 |
KEGGi | rno:60399 |
UCSCi | RGD:620566, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF115249 mRNA Translation: AAF15395.1 AF233649 mRNA Translation: AAF35912.1 |
RefSeqi | NP_068543.2, NM_021775.2 XP_006242725.1, XM_006242663.3 XP_006242726.1, XM_006242664.3 XP_006242727.1, XM_006242665.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 248819, 1 interactor |
STRINGi | 10116.ENSRNOP00000028380 |
Chemistry databases
GuidetoPHARMACOLOGYi | 279 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q9JKM5, 1 site |
iPTMneti | Q9JKM5 |
PhosphoSitePlusi | Q9JKM5 |
Proteomic databases
PaxDbi | Q9JKM5 |
PRIDEi | Q9JKM5 |
Genome annotation databases
Ensembli | ENSRNOT00000028380; ENSRNOP00000028380; ENSRNOG00000020901 |
GeneIDi | 60399 |
KEGGi | rno:60399 |
UCSCi | RGD:620566, rat |
Organism-specific databases
CTDi | 53637 |
RGDi | 620566, S1pr5 |
Phylogenomic databases
eggNOGi | ENOG502QSWN, Eukaryota |
GeneTreei | ENSGT00970000193365 |
HOGENOMi | CLU_047979_1_0_1 |
InParanoidi | Q9JKM5 |
OMAi | MVTMRPY |
OrthoDBi | 981486at2759 |
PhylomeDBi | Q9JKM5 |
TreeFami | TF330052 |
Enzyme and pathway databases
Reactomei | R-RNO-418594, G alpha (i) signalling events R-RNO-419408, Lysosphingolipid and LPA receptors |
Miscellaneous databases
PROi | PR:Q9JKM5 |
Gene expression databases
Bgeei | ENSRNOG00000020901, Expressed in brain and 22 other tissues |
Genevisiblei | Q9JKM5, RN |
Family and domain databases
InterProi | View protein in InterPro IPR005386, EDG8_S1P_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR004061, S1P_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01561, EDG8RECEPTOR PR00237, GPCRRHODOPSN PR01523, S1PRECEPTOR |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S1PR5_RAT | |
Accessioni | Q9JKM5Primary (citable) accession number: Q9JKM5 Secondary accession number(s): Q9QY79 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries