UniProtKB - Q9JKD9 (ZBT32_MOUSE)

BLAST

>sp|Q9JKD9|ZBT32_MOUSE Zinc finger and BTB domain-containing protein 32 OS=Mus musculus OX=10090 GN=Zbtb32 PE=1 SV=1
MPQTPTRLVSPYGSDRLVQLAARLRPALCDTLITVGGLEFPAHSLVLAGASPRLGCRGRW
ALVEDISPSTFAQILTFVYGESIELQPGELGDLEEAAKALGVQALEEACQRAQKGKDEDE
LDPGLKRHQQSEDFMRGSERGLGSPGEKQKPEKDFRSNGREQEMSHKHKAPGERPEMAGA
TRMMSSEEVMRGIESHKGSEESLRGCPDPLSPPGSLLTSLIPRPWWAEVPRLGEGQSALW
SILLWPSRYGAPFSHSTPITAAWQVRPQDQRIPLTLNHSKALWSQNQLASSSPTPGSFPQ
GTESLSPWQIETSGQGFTGTLATCVSQERTLNCPSHQHPPLPSPARSRPYSCSVCGKRFS
LKHQMETHYRVHTGEKPFSCSLCPQRSRDFSAMTKHLRTHGAAPYRCPLCRAGCPSLASM
QAHMRGHSPSRLPPGWTIRSTFLYSSSRPTRASSSPGSPTSSAAT

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History
Entry version 133 (07 Nov 2018)
Sequence version 1 (01 Oct 2000)
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Protein

Zinc finger and BTB domain-containing protein 32

Gene

Zbtb32

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor (By similarity). Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells.By similarity1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	350 – 372	C2H2-type 1PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	378 – 400	C2H2-type 2PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	405 – 427	C2H2-type 3PROSITE-ProRule annotationAdd BLAST	23

GO - Molecular functionⁱ

DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 20544958
transcription corepressor activity
Source: MGI
Inferred from direct assayⁱ
- 10755619
zinc ion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 20544958

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

hemopoiesis
Source: MGI
Inferred from mutant phenotypeⁱ
- 15542853
regulation of cytokine production
Source: MGI
Inferred from mutant phenotypeⁱ
- 15542853
T cell proliferation
Source: MGI
Inferred from mutant phenotypeⁱ
- 15542853

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Repressor
Biological process	Transcription, Transcription regulation
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex

Reactomeⁱ

R-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Zinc finger and BTB domain-containing protein 32 Alternative name(s): Repressor of GATA Testis zinc finger protein
Gene namesⁱ	Name:Zbtb32 Synonyms:Rog, Tzfp
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 7

Organism-specific databases

MGIⁱ	MGI:1891838 Zbtb32

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000273418	1 – 465	Zinc finger and BTB domain-containing protein 32Add BLAST		465

Proteomic databases

EPDⁱ	Q9JKD9
PaxDbⁱ	Q9JKD9
PRIDEⁱ	Q9JKD9

PTM databases

iPTMnetⁱ	Q9JKD9
PhosphoSitePlusⁱ	Q9JKD9

Expressionⁱ

Tissue specificityⁱ

Isoform 1 is testis-specific and is not expressed in lymphoid organs such as thymus or spleen. Isoform 2 is expressed in both B-and T-lymphoid cells.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000006310 Expressed in 75 organ(s), highest expression level in testis
CleanExⁱ	MM_ZBTB32
ExpressionAtlasⁱ	Q9JKD9 baseline and differential
Genevisibleⁱ	Q9JKD9 MM

Interactionⁱ

Subunit structureⁱ

Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16 (By similarity). Interacts with GATA3.By similarity1 Publication

Protein-protein interaction databases

BioGridⁱ	208389, 1 interactor
STRINGⁱ	10090.ENSMUSP00000103786

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	349 – 351	Combined sources	3
Beta strandⁱ	353 – 355	Combined sources	3
Beta strandⁱ	358 – 360	Combined sources	3
Helixⁱ	362 – 369	Combined sources	8
Beta strandⁱ	381 – 384	Combined sources	4
Beta strandⁱ	386 – 389	Combined sources	4
Helixⁱ	390 – 399	Combined sources	10
Beta strandⁱ	405 – 407	Combined sources	3
Turnⁱ	408 – 411	Combined sources	4
Beta strandⁱ	412 – 415	Combined sources	4
Helixⁱ	417 – 424	Combined sources	8

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9JKD9
SMRⁱ	Q9JKD9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

Q9JKD9

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	29 – 87	BTBPROSITE-ProRule annotationAdd BLAST		59

Domainⁱ

The C-terminal zinc finger domain functions as a transcriptional transactivator.

The BTB (POZ) domain possesses repressor activity.

Sequence similaritiesⁱ

Belongs to the krueppel C2H2-type zinc-finger protein family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	350 – 372	C2H2-type 1PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	378 – 400	C2H2-type 2PROSITE-ProRule annotationAdd BLAST	23
Zinc fingerⁱ	405 – 427	C2H2-type 3PROSITE-ProRule annotationAdd BLAST	23

Keywords - Domainⁱ

Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG1721 Eukaryota COG5048 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063338
HOGENOMⁱ	HOG000060112
HOVERGENⁱ	HBG079944
InParanoidⁱ	Q9JKD9
KEGG Orthology (KO) More...KOⁱ	K10506
OMAⁱ	WWAEAPW
PhylomeDBⁱ	Q9JKD9

Family and domain databases

InterProⁱ	View protein in InterPro IPR000210 BTB/POZ_dom IPR011333 SKP1/BTB/POZ_sf IPR036236 Znf_C2H2_sf IPR013087 Znf_C2H2_type
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00651 BTB, 1 hit PF00096 zf-C2H2, 1 hit
SMARTⁱ	View protein in SMART SM00225 BTB, 1 hit SM00355 ZnF_C2H2, 3 hits
SUPFAMⁱ	SSF54695 SSF54695, 1 hit SSF57667 SSF57667, 1 hit
PROSITEⁱ	View protein in PROSITE PS50097 BTB, 1 hit PS00028 ZINC_FINGER_C2H2_1, 2 hits PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform 1 (identifier: Q9JKD9-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPQTPTRLVS PYGSDRLVQL AARLRPALCD TLITVGGLEF PAHSLVLAGA 
        60         70         80         90        100
SPRLGCRGRW ALVEDISPST FAQILTFVYG ESIELQPGEL GDLEEAAKAL 
       110        120        130        140        150
GVQALEEACQ RAQKGKDEDE LDPGLKRHQQ SEDFMRGSER GLGSPGEKQK 
       160        170        180        190        200
PEKDFRSNGR EQEMSHKHKA PGERPEMAGA TRMMSSEEVM RGIESHKGSE 
       210        220        230        240        250
ESLRGCPDPL SPPGSLLTSL IPRPWWAEVP RLGEGQSALW SILLWPSRYG 
       260        270        280        290        300
APFSHSTPIT AAWQVRPQDQ RIPLTLNHSK ALWSQNQLAS SSPTPGSFPQ 
       310        320        330        340        350
GTESLSPWQI ETSGQGFTGT LATCVSQERT LNCPSHQHPP LPSPARSRPY 
       360        370        380        390        400
SCSVCGKRFS LKHQMETHYR VHTGEKPFSC SLCPQRSRDF SAMTKHLRTH 
       410        420        430        440        450
GAAPYRCPLC RAGCPSLASM QAHMRGHSPS RLPPGWTIRS TFLYSSSRPT 
       460 
RASSSPGSPT SSAAT

Length:465

Mass (Da):50,828

Last modified:October 1, 2000 - v1

Checksum:ⁱD72E83AB3661F403

Isoform 2 (identifier: Q9JKD9-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-364: Missing.

« Hide

        10         20         30         40         50
METHYRVHTG EKPFSCSLCP QRSRDFSAMT KHLRTHGAAP YRCPLCRAGC 
        60         70         80         90        100
PSLASMQAHM RGHSPSRLPP GWTIRSTFLY SSSRPTRASS SPGSPTSSAA 

T

Note: No experimental confirmation available.

Show »

Length:101

Mass (Da):11,021

Checksum:ⁱF4F882ED6B5723FC

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	B2RQ06	B2RQ06_MOUSE		Zinc finger and BTB domain containi... Zinc finger and BTB domain containing 32 (Zinc finger and BTB domain-containing protein 32)	Zbtb32	465	Annotation score:

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_022552	1 – 364	Missing in isoform 2. 1 PublicationAdd BLAST		364

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF241232 mRNA Translation: AAF61244.1 AY015272 Genomic DNA Translation: AAK13198.1 AK015881 mRNA Translation: BAB30015.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS21102.1 [Q9JKD9-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_067372.2, NM_021397.2
UniGeneⁱ	Mm.116789 Mm.452815

Genome annotation databases

Ensemblⁱ	ENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310 [Q9JKD9-2]
GeneIDⁱ	58206
KEGGⁱ	mmu:58206
UCSC genome browser More...UCSCⁱ	uc009gfi.1 mouse [Q9JKD9-2]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9JKD9	Isoform 2 of Zinc finger and BTB domain-containing protein 32		MOUSE		101	UniRef90_Q9JKD9
UPI000A305AA6		MUSPA		465
UPI000A31AFF0		MUSCR		465
Zinc finger and BTB domain containing 32		MOUSE		465

Protein	Similar proteins		Species	Score	Length	Source
Q9JKD9	Isoform 2 of Zinc finger and BTB domain-containing protein 32		MOUSE		101	UniRef50_Q9JKD9
Uncharacterized protein		NEOLE		523
RCG53796		RAT		483
Zinc finger and BTB domain-containing protein 32		HETGA		483
Uncharacterized protein		PAPAN		464
+29

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF241232 mRNA Translation: AAF61244.1 AY015272 Genomic DNA Translation: AAK13198.1 AK015881 mRNA Translation: BAB30015.1
CCDSⁱ	CCDS21102.1 [Q9JKD9-1]
RefSeqⁱ	NP_067372.2, NM_021397.2
UniGeneⁱ	Mm.116789 Mm.452815

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2KVF	NMR	-	A	348-374	[»]
	2KVG	NMR	-	A	402-428	[»]
	2KVH	NMR	-	A	375-401	[»]
ProteinModelPortalⁱ	Q9JKD9
SMRⁱ	Q9JKD9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	208389, 1 interactor
STRINGⁱ	10090.ENSMUSP00000103786

PTM databases

iPTMnetⁱ	Q9JKD9
PhosphoSitePlusⁱ	Q9JKD9

Proteomic databases

EPDⁱ	Q9JKD9
PaxDbⁱ	Q9JKD9
PRIDEⁱ	Q9JKD9

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310 [Q9JKD9-2]
GeneIDⁱ	58206
KEGGⁱ	mmu:58206
UCSCⁱ	uc009gfi.1 mouse [Q9JKD9-2]

Organism-specific databases

CTDⁱ	27033
MGIⁱ	MGI:1891838 Zbtb32

Phylogenomic databases

eggNOGⁱ	KOG1721 Eukaryota COG5048 LUCA
GeneTreeⁱ	ENSGT00530000063338
HOGENOMⁱ	HOG000060112
HOVERGENⁱ	HBG079944
InParanoidⁱ	Q9JKD9
KOⁱ	K10506
OMAⁱ	WWAEAPW
PhylomeDBⁱ	Q9JKD9

Enzyme and pathway databases

Reactomeⁱ	R-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex

Reactomeⁱ

R-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex

Miscellaneous databases

EvolutionaryTraceⁱ	Q9JKD9
Protein Ontology More...PROⁱ	PR:Q9JKD9
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000006310 Expressed in 75 organ(s), highest expression level in testis
CleanExⁱ	MM_ZBTB32
ExpressionAtlasⁱ	Q9JKD9 baseline and differential
Genevisibleⁱ	Q9JKD9 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR000210 BTB/POZ_dom IPR011333 SKP1/BTB/POZ_sf IPR036236 Znf_C2H2_sf IPR013087 Znf_C2H2_type
Pfamⁱ	View protein in Pfam PF00651 BTB, 1 hit PF00096 zf-C2H2, 1 hit
SMARTⁱ	View protein in SMART SM00225 BTB, 1 hit SM00355 ZnF_C2H2, 3 hits
SUPFAMⁱ	SSF54695 SSF54695, 1 hit SSF57667 SSF57667, 1 hit
PROSITEⁱ	View protein in PROSITE PS50097 BTB, 1 hit PS00028 ZINC_FINGER_C2H2_1, 2 hits PS50157 ZINC_FINGER_C2H2_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ZBT32_MOUSE
Accessionⁱ	Q9JKD9Primary (citable) accession number: Q9JKD9 Secondary accession number(s): Q9D523
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
	Last sequence update:	October 1, 2000
	Last modified:	November 7, 2018
	This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - Q9JKD9 (ZBT32_MOUSE)

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Zinc finger and BTB domain-containing protein 32

Zbtb32

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ