UniProtKB - Q9JKD9 (ZBT32_MOUSE)
Zinc finger and BTB domain-containing protein 32
Zbtb32
Functioni
DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor (By similarity).
Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells.
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 350 – 372 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 378 – 400 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 405 – 427 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific double-stranded DNA binding Source: MGI
- transcription corepressor activity Source: MGI
- zinc ion binding Source: UniProtKB
GO - Biological processi
- hemopoiesis Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- regulation of cytokine production Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- T cell proliferation Source: MGI
Keywordsi
Molecular function | Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger and BTB domain-containing protein 32Alternative name(s): Repressor of GATA Testis zinc finger protein |
Gene namesi | Name:Zbtb32 Synonyms:Rog, Tzfp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1891838, Zbtb32 |
VEuPathDBi | HostDB:ENSMUSG00000006310 |
Subcellular locationi
Nucleus
Note: Located in nuclear speckles.By similarity
Nucleus
- nuclear chromosome Source: MGI
- nucleoplasm Source: GO_Central
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000273418 | 1 – 465 | Zinc finger and BTB domain-containing protein 32Add BLAST | 465 |
Proteomic databases
EPDi | Q9JKD9 |
MaxQBi | Q9JKD9 |
PaxDbi | Q9JKD9 |
PRIDEi | Q9JKD9 |
ProteomicsDBi | 275053 [Q9JKD9-1] 275054 [Q9JKD9-2] |
PTM databases
iPTMneti | Q9JKD9 |
PhosphoSitePlusi | Q9JKD9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000006310, Expressed in spermatocyte and 118 other tissues |
Genevisiblei | Q9JKD9, MM |
Interactioni
Subunit structurei
Homodimer (via PTB domain).
Interacts with the N-terminal of FANCC.
Interacts with ZBTB16 (By similarity).
Interacts with GATA3.
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 208389, 1 interactor |
STRINGi | 10090.ENSMUSP00000103786 |
Miscellaneous databases
RNActi | Q9JKD9, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9JKD9 |
BMRBi | Q9JKD9 |
SMRi | Q9JKD9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9JKD9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 87 | BTBPROSITE-ProRule annotationAdd BLAST | 59 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 111 – 179 | DisorderedSequence analysisAdd BLAST | 69 | |
Regioni | 285 – 310 | DisorderedSequence analysisAdd BLAST | 26 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 111 – 135 | Basic and acidic residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 143 – 175 | Basic and acidic residuesSequence analysisAdd BLAST | 33 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 350 – 372 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 378 – 400 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 405 – 427 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162716 |
HOGENOMi | CLU_002678_42_18_1 |
InParanoidi | Q9JKD9 |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q9JKD9 |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit PF00096, zf-C2H2, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 2 hits PS50157, ZINC_FINGER_C2H2_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPQTPTRLVS PYGSDRLVQL AARLRPALCD TLITVGGLEF PAHSLVLAGA
60 70 80 90 100
SPRLGCRGRW ALVEDISPST FAQILTFVYG ESIELQPGEL GDLEEAAKAL
110 120 130 140 150
GVQALEEACQ RAQKGKDEDE LDPGLKRHQQ SEDFMRGSER GLGSPGEKQK
160 170 180 190 200
PEKDFRSNGR EQEMSHKHKA PGERPEMAGA TRMMSSEEVM RGIESHKGSE
210 220 230 240 250
ESLRGCPDPL SPPGSLLTSL IPRPWWAEVP RLGEGQSALW SILLWPSRYG
260 270 280 290 300
APFSHSTPIT AAWQVRPQDQ RIPLTLNHSK ALWSQNQLAS SSPTPGSFPQ
310 320 330 340 350
GTESLSPWQI ETSGQGFTGT LATCVSQERT LNCPSHQHPP LPSPARSRPY
360 370 380 390 400
SCSVCGKRFS LKHQMETHYR VHTGEKPFSC SLCPQRSRDF SAMTKHLRTH
410 420 430 440 450
GAAPYRCPLC RAGCPSLASM QAHMRGHSPS RLPPGWTIRS TFLYSSSRPT
460
RASSSPGSPT SSAAT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB2RQ06 | B2RQ06_MOUSE | Zinc finger and BTB domain containi... | Zbtb32 | 465 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_022552 | 1 – 364 | Missing in isoform 2. 1 PublicationAdd BLAST | 364 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF241232 mRNA Translation: AAF61244.1 AY015272 Genomic DNA Translation: AAK13198.1 AK015881 mRNA Translation: BAB30015.1 |
CCDSi | CCDS21102.1 [Q9JKD9-1] |
RefSeqi | NP_067372.2, NM_021397.2 |
Genome annotation databases
Ensembli | ENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310 [Q9JKD9-2] |
GeneIDi | 58206 |
KEGGi | mmu:58206 |
UCSCi | uc009gfi.1, mouse [Q9JKD9-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF241232 mRNA Translation: AAF61244.1 AY015272 Genomic DNA Translation: AAK13198.1 AK015881 mRNA Translation: BAB30015.1 |
CCDSi | CCDS21102.1 [Q9JKD9-1] |
RefSeqi | NP_067372.2, NM_021397.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KVF | NMR | - | A | 348-374 | [»] | |
2KVG | NMR | - | A | 402-428 | [»] | |
2KVH | NMR | - | A | 375-401 | [»] | |
AlphaFoldDBi | Q9JKD9 | |||||
BMRBi | Q9JKD9 | |||||
SMRi | Q9JKD9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 208389, 1 interactor |
STRINGi | 10090.ENSMUSP00000103786 |
PTM databases
iPTMneti | Q9JKD9 |
PhosphoSitePlusi | Q9JKD9 |
Proteomic databases
EPDi | Q9JKD9 |
MaxQBi | Q9JKD9 |
PaxDbi | Q9JKD9 |
PRIDEi | Q9JKD9 |
ProteomicsDBi | 275053 [Q9JKD9-1] 275054 [Q9JKD9-2] |
Protocols and materials databases
Antibodypediai | 35117, 181 antibodies from 24 providers |
DNASUi | 58206 |
Genome annotation databases
Ensembli | ENSMUST00000108150; ENSMUSP00000103785; ENSMUSG00000006310 [Q9JKD9-2] |
GeneIDi | 58206 |
KEGGi | mmu:58206 |
UCSCi | uc009gfi.1, mouse [Q9JKD9-2] |
Organism-specific databases
CTDi | 27033 |
MGIi | MGI:1891838, Zbtb32 |
VEuPathDBi | HostDB:ENSMUSG00000006310 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162716 |
HOGENOMi | CLU_002678_42_18_1 |
InParanoidi | Q9JKD9 |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q9JKD9 |
Miscellaneous databases
BioGRID-ORCSi | 58206, 7 hits in 74 CRISPR screens |
ChiTaRSi | Zbtb32, mouse |
EvolutionaryTracei | Q9JKD9 |
PROi | PR:Q9JKD9 |
RNActi | Q9JKD9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000006310, Expressed in spermatocyte and 118 other tissues |
Genevisiblei | Q9JKD9, MM |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit PF00096, zf-C2H2, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 2 hits PS50157, ZINC_FINGER_C2H2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ZBT32_MOUSE | |
Accessioni | Q9JKD9Primary (citable) accession number: Q9JKD9 Secondary accession number(s): Q9D523 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2007 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families