UniProtKB - Q9JKD8 (TBX21_MOUSE)
T-box transcription factor TBX21
Tbx21
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 304 | Essential for its interaction with RUNX1 and its ability to inhibit RUNX1 transcriptional activity and suppress TH17 lineage development1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 140 – 325 | T-boxPROSITE-ProRule annotationAdd BLAST | 186 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- cell fate specification Source: GO_Central
- cellular response to organic substance Source: MGI
- heart looping Source: GO_Central
- lymphocyte migration Source: UniProtKB
- negative regulation of interleukin-2 production Source: UniProtKB
- negative regulation of T-helper 17 cell differentiation Source: UniProtKB
- negative regulation of T-helper 17 cell lineage commitment Source: UniProtKB
- negative regulation of T-helper 2 cell cytokine production Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of isotype switching to IgG isotypes Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- regulation of immune response Source: MGI
- regulation of T cell differentiation Source: UniProtKB
- response to virus Source: Ensembl
- T cell differentiation Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: T-box transcription factor TBX21Short name: T-box protein 21 Alternative name(s): T-cell-specific T-box transcription factor T-bet Transcription factor TBLYM |
Gene namesi | Name:Tbx21 Synonyms:Tbet, Tblym |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1888984, Tbx21 |
Subcellular locationi
Nucleus
- Nucleus 4 Publications
Nucleus
- nuclear chromatin Source: GO_Central
- nucleus Source: UniProtKB
Other locations
- neuronal cell body Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | S → A: No loss in its ability to induce IFN-gamma. Significant reduction in its ability to induce IFN-gamma and reduced ability to promote addition of permissive chromatin-remodeling mark H3K4Me2 to the IFNG promoter region; when associated with A-76 and A-224. 1 Publication | 1 | |
Mutagenesisi | 55 | T → A: No loss in its ability to induce IFN-gamma. 1 Publication | 1 | |
Mutagenesisi | 76 | Y → A: No loss in its ability to induce IFN-gamma. Significant reduction in its ability to induce IFN-gamma and reduced ability to promote addition of permissive chromatin-remodeling mark H3K4Me2 to the IFNG promoter region; when associated with A-52 and A-224. 1 Publication | 1 | |
Mutagenesisi | 117 | Y → A: No loss in its ability to induce IFN-gamma. 1 Publication | 1 | |
Mutagenesisi | 208 | K → R: No effect on DNA-binding or its nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 219 | Y → F: Significant loss of ABL1-mediated phosphorylation and loss of transcriptional activator activity; when associated with F-225 and F-304. 1 Publication | 1 | |
Mutagenesisi | 224 | S → A: No loss in its ability to induce IFN-gamma. Significant reduction in its ability to induce IFN-gamma and reduced ability to promote addition of permissive chromatin-remodeling mark H3K4Me2 to the IFNG promoter region; when associated with A-52 and A-76. 1 Publication | 1 | |
Mutagenesisi | 265 | Y → F: No loss of interaction with RUNX1. 1 Publication | 1 | |
Mutagenesisi | 265 | Y → F: Significant loss of ABL1-mediated phosphorylation and loss of transcriptional activator activity; when associated with F-219 and F-304. 1 Publication | 1 | |
Mutagenesisi | 302 | T → A: Loss of phosphorylation and its ability to interact with NFATC2. Loss of its ability to suppress IL-2 and Th2 cytokine production. No loss of DNA-binding and no loss its ability to activate IFN-gamma transcription. 1 Publication | 1 | |
Mutagenesisi | 304 | Y → F: Loss of interaction with RUNX1 and loss of its ability to inhibit RUNX1 transcriptional activity and suppress TH17 lineage development. Significant loss of ABL1-mediated phosphorylation and loss of transcriptional activator activity; when associated with F-219 and F-225. 2 Publications | 1 | |
Mutagenesisi | 310 | T → A: No loss of phospshorylation. 1 Publication | 1 | |
Mutagenesisi | 313 | K → R: Significant loss of ubiquitination. Loss of phosphorylation at T-302 causing loss of its ability to interact with NFATC2. Loss its ability to activate IFN-gamma transcription due to loss of DNA-binding activity. Loss of its ability to suppress IL-2 and Th2 cytokine production. Increased protein stability. Localization seen in both the nucleus and cytoplasm. No loss of interaction with GATA3 and RELA. 1 Publication | 1 | |
Mutagenesisi | 321 | K → R: No effect on DNA-binding or its nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 437 | Y → F: No loss of interaction with ITK and GATA3. 1 Publication | 1 | |
Mutagenesisi | 508 | S → A: No loss of interaction with RUNX1. No loss in its ability to induce IFN-gamma. 2 Publications | 1 | |
Mutagenesisi | 525 | Y → F: No loss of interaction with RUNX1. Loss of interaction with ITK and GATA3. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184454 | 1 – 530 | T-box transcription factor TBX21Add BLAST | 530 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 52 | Phosphoserine1 Publication | 1 | |
Modified residuei | 55 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 76 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 117 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 219 | Phosphotyrosine; by ABL11 Publication | 1 | |
Modified residuei | 224 | Phosphoserine1 Publication | 1 | |
Modified residuei | 265 | Phosphotyrosine; by ABL11 Publication | 1 | |
Modified residuei | 302 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 304 | Phosphotyrosine; by ABL11 Publication | 1 | |
Cross-linki | 313 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 508 | Phosphoserine1 Publication | 1 | |
Modified residuei | 525 | Phosphotyrosine; by ITK1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9JKD8 |
MaxQBi | Q9JKD8 |
PaxDbi | Q9JKD8 |
PRIDEi | Q9JKD8 |
PTM databases
iPTMneti | Q9JKD8 |
PhosphoSitePlusi | Q9JKD8 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000001444, Expressed in head and 76 other tissues |
Genevisiblei | Q9JKD8, MM |
Interactioni
Subunit structurei
Interacts with RUNX1 and RUNX3 (PubMed:21151104).
Interacts with ITK (PubMed:15662016). The phosphorylated form (at Tyr-525) interacts with GATA3 (PubMed:15662016, PubMed:21690296, PubMed:23616576).
Interacts with ABL1 (PubMed:21690296).
Interacts with RELA (PubMed:23616576). The phosphorylated form (at Thr-302) interacts with NFATC2 (PubMed:23616576).
Interacts with KDM6B (PubMed:21095589).
Interacts with SMARCA4 in a KDM6B-dependent manner (PubMed:21095589).
Interacts with CCTN1 and CDK9 (PubMed:27292648).
Interacts with USP10 (By similarity).
By similarity6 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 208321, 9 interactors |
IntActi | Q9JKD8, 3 interactors |
STRINGi | 10090.ENSMUSP00000001484 |
Miscellaneous databases
RNActi | Q9JKD8, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9JKD8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | KOG3585, Eukaryota |
GeneTreei | ENSGT00940000160397 |
HOGENOMi | CLU_014430_8_2_1 |
InParanoidi | Q9JKD8 |
OMAi | WTEITPI |
OrthoDBi | 374561at2759 |
PhylomeDBi | Q9JKD8 |
TreeFami | TF106341 |
Family and domain databases
CDDi | cd00182, TBOX, 1 hit |
Gene3Di | 2.60.40.820, 1 hit |
InterProi | View protein in InterPro IPR008967, p53-like_TF_DNA-bd IPR036960, T-box_sf IPR001699, TF_T-box IPR018186, TF_T-box_CS |
PANTHERi | PTHR11267, PTHR11267, 1 hit |
Pfami | View protein in Pfam PF00907, T-box, 1 hit |
PRINTSi | PR00937, TBOX |
SMARTi | View protein in SMART SM00425, TBOX, 1 hit |
SUPFAMi | SSF49417, SSF49417, 1 hit |
PROSITEi | View protein in PROSITE PS01283, TBOX_1, 1 hit PS01264, TBOX_2, 1 hit PS50252, TBOX_3, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGIVEPGCGD MLTGTEPMPS DEGRGPGADQ QHRFFYPEPG AQDPTDRRAG
60 70 80 90 100
SSLGTPYSGG ALVPAAPGRF LGSFAYPPRA QVAGFPGPGE FFPPPAGAEG
110 120 130 140 150
YPPVDGYPAP DPRAGLYPGP REDYALPAGL EVSGKLRVAL SNHLLWSKFN
160 170 180 190 200
QHQTEMIITK QGRRMFPFLS FTVAGLEPTS HYRMFVDVVL VDQHHWRYQS
210 220 230 240 250
GKWVQCGKAE GSMPGNRLYV HPDSPNTGAH WMRQEVSFGK LKLTNNKGAS
260 270 280 290 300
NNVTQMIVLQ SLHKYQPRLH IVEVNDGEPE AACSASNTHV FTFQETQFIA
310 320 330 340 350
VTAYQNAEIT QLKIDNNPFA KGFRENFESM YASVDTSVPS PPGPNCQLLG
360 370 380 390 400
GDPFSPLLSN QYPVPSRFYP DLPGQPKDMI SQPYWLGTPR EHSYEAEFRA
410 420 430 440 450
VSMKPTLLPS APGPTVPYYR GQDVLAPGAG WPVAPQYPPK MSPAGWFRPM
460 470 480 490 500
RTLPMDPGLG SSEEQGSSPS LWPEVTSLQP EPSDSGLGEG DTKRRRISPY
510 520 530
PSSGDSSSPA GAPSPFDKET EGQFYNYFPN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 482 | P → S in AAF00056 (PubMed:11087660).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF241242 mRNA Translation: AAF61242.1 AF093099 mRNA Translation: AAF00056.1 AK156271 mRNA Translation: BAE33650.1 CH466556 Genomic DNA Translation: EDL16071.1 BC137986 mRNA Translation: AAI37987.1 BC137988 mRNA Translation: AAI37989.1 |
CCDSi | CCDS25315.1 |
RefSeqi | NP_062380.2, NM_019507.2 |
Genome annotation databases
Ensembli | ENSMUST00000001484; ENSMUSP00000001484; ENSMUSG00000001444 |
GeneIDi | 57765 |
KEGGi | mmu:57765 |
UCSCi | uc007ldr.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF241242 mRNA Translation: AAF61242.1 AF093099 mRNA Translation: AAF00056.1 AK156271 mRNA Translation: BAE33650.1 CH466556 Genomic DNA Translation: EDL16071.1 BC137986 mRNA Translation: AAI37987.1 BC137988 mRNA Translation: AAI37989.1 |
CCDSi | CCDS25315.1 |
RefSeqi | NP_062380.2, NM_019507.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5T1J | X-ray | 2.95 | A/B | 135-326 | [»] | |
SMRi | Q9JKD8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 208321, 9 interactors |
IntActi | Q9JKD8, 3 interactors |
STRINGi | 10090.ENSMUSP00000001484 |
PTM databases
iPTMneti | Q9JKD8 |
PhosphoSitePlusi | Q9JKD8 |
Proteomic databases
EPDi | Q9JKD8 |
MaxQBi | Q9JKD8 |
PaxDbi | Q9JKD8 |
PRIDEi | Q9JKD8 |
Protocols and materials databases
Antibodypediai | 4021, 674 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000001484; ENSMUSP00000001484; ENSMUSG00000001444 |
GeneIDi | 57765 |
KEGGi | mmu:57765 |
UCSCi | uc007ldr.2, mouse |
Organism-specific databases
CTDi | 30009 |
MGIi | MGI:1888984, Tbx21 |
Phylogenomic databases
eggNOGi | KOG3585, Eukaryota |
GeneTreei | ENSGT00940000160397 |
HOGENOMi | CLU_014430_8_2_1 |
InParanoidi | Q9JKD8 |
OMAi | WTEITPI |
OrthoDBi | 374561at2759 |
PhylomeDBi | Q9JKD8 |
TreeFami | TF106341 |
Miscellaneous databases
BioGRID-ORCSi | 57765, 0 hits in 17 CRISPR screens |
ChiTaRSi | Tbx21, mouse |
PROi | PR:Q9JKD8 |
RNActi | Q9JKD8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001444, Expressed in head and 76 other tissues |
Genevisiblei | Q9JKD8, MM |
Family and domain databases
CDDi | cd00182, TBOX, 1 hit |
Gene3Di | 2.60.40.820, 1 hit |
InterProi | View protein in InterPro IPR008967, p53-like_TF_DNA-bd IPR036960, T-box_sf IPR001699, TF_T-box IPR018186, TF_T-box_CS |
PANTHERi | PTHR11267, PTHR11267, 1 hit |
Pfami | View protein in Pfam PF00907, T-box, 1 hit |
PRINTSi | PR00937, TBOX |
SMARTi | View protein in SMART SM00425, TBOX, 1 hit |
SUPFAMi | SSF49417, SSF49417, 1 hit |
PROSITEi | View protein in PROSITE PS01283, TBOX_1, 1 hit PS01264, TBOX_2, 1 hit PS50252, TBOX_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TBX21_MOUSE | |
Accessioni | Q9JKD8Primary (citable) accession number: Q9JKD8 Secondary accession number(s): Q3U150, Q9R0A6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references