UniProtKB - Q9JK71 (MAGI3_RAT)
Protein
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
Gene
Magi3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 123 – 130 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- frizzled binding Source: RGD
- molecular adaptor activity Source: InterPro
GO - Biological processi
- positive regulation of JUN kinase activity Source: RGD
- regulation of JNK cascade Source: InterPro
- signal transduction Source: GO_Central
Keywordsi
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3Alternative name(s): Membrane-associated guanylate kinase inverted 3 Short name: MAGI-3 Scaffolding-like protein |
Gene namesi | Name:Magi3 Synonyms:Slipr |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621362, Magi3 |
Subcellular locationi
Nucleus
Plasma membrane
Other locations
Note: Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- bicellular tight junction Source: UniProtKB-SubCell
- cell-cell junction Source: RGD
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Nucleus, Tight junctionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000341409 | 1 – 1470 | Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3Add BLAST | 1470 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 236 | PhosphoserineBy similarity | 1 | |
Modified residuei | 598 | PhosphoserineBy similarity | 1 | |
Modified residuei | 702 | PhosphoserineCombined sources | 1 | |
Modified residuei | 833 | PhosphoserineCombined sources | 1 | |
Modified residuei | 916 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1321 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9JK71 |
PRIDEi | Q9JK71 |
PTM databases
iPTMneti | Q9JK71 |
PhosphoSitePlusi | Q9JK71 |
Interactioni
Subunit structurei
Interacts with ADRB1, ADGRB1, LPAR2/EDG4, FZD4, FZD7, GRIN2B, TGFA and VANGL2 (By similarity).
Interacts with PTEN.
Interacts with ADRB1, PTPRB and unidentified tyrosine phosphorylated proteins.
Interacts with DLL1 (By similarity).
Interacts with PRRG4 (via cytoplasmic domain) (By similarity).
By similarity3 PublicationsBinary interactionsi
Hide detailsQ9JK71
With | #Exp. | IntAct |
---|---|---|
Adrb1 [P18090] | 3 | EBI-696226,EBI-991303 |
PTEN [P60484] from Homo sapiens. | 3 | EBI-696226,EBI-696162 |
GO - Molecular functioni
- frizzled binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 251439, 3 interactors |
IntActi | Q9JK71, 2 interactors |
STRINGi | 10116.ENSRNOP00000026952 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 18 – 108 | PDZ 1PROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 116 – 290 | Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST | 175 | |
Domaini | 296 – 329 | WW 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 342 – 375 | WW 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 413 – 495 | PDZ 2PROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 581 – 657 | PDZ 3PROSITE-ProRule annotationAdd BLAST | 77 | |
Domaini | 729 – 811 | PDZ 4PROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 852 – 939 | PDZ 5PROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 1022 – 1104 | PDZ 6PROSITE-ProRule annotationAdd BLAST | 83 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 18 – 108 | Interaction with ADRB1 and TGFA1 PublicationAdd BLAST | 91 | |
Regioni | 413 – 495 | Interaction with PTENBy similarityAdd BLAST | 83 | |
Regioni | 729 – 811 | Interaction with ADGRB1By similarityAdd BLAST | 83 | |
Regioni | 852 – 939 | Interaction with LPAR2 and GRIN2BBy similarityAdd BLAST | 88 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 6 – 9 | Poly-Lys | 4 | |
Compositional biasi | 240 – 245 | Poly-Glu | 6 |
Sequence similaritiesi
Belongs to the MAGUK family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0707, Eukaryota KOG3209, Eukaryota |
InParanoidi | Q9JK71 |
OrthoDBi | 284488at2759 |
PhylomeDBi | Q9JK71 |
Family and domain databases
CDDi | cd00201, WW, 2 hits |
Gene3Di | 2.30.42.10, 6 hits |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR020590, Guanylate_kinase_CS IPR030035, MAGI3 IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR10316:SF10, PTHR10316:SF10, 1 hit |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 4 hits PF00397, WW, 2 hits |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 6 hits SM00456, WW, 2 hits |
SUPFAMi | SSF50156, SSF50156, 6 hits SSF51045, SSF51045, 2 hits SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00856, GUANYLATE_KINASE_1, 1 hit PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 6 hits PS01159, WW_DOMAIN_1, 2 hits PS50020, WW_DOMAIN_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9JK71-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKTLKKKKH WLSKVQECAV SWAGPPGDLG AEIRGGAERG EFPYLGRLRD
60 70 80 90 100
EPGGGGGTCC VVSGKAPSPG DVLLEVNGTP VSGLTNRDTL AVIRHFREPI
110 120 130 140 150
RLKTVKPGKV INKDLRHYLS LQFQKGSIDH KLQQVIRDNL YLITIPCTTR
160 170 180 190 200
APRDGEVPGV DYNFISVEQF KALEESGALL ESGTYDGNFY GTPKPPAEPS
210 220 230 240 250
PFQPDPVDQV LFDNEFDTES QRKRTTSVSK MERMDSSLPE EEEDEDKEAV
260 270 280 290 300
NGSGSMETRE MHSESSDCWM KTVPSYNQTN RSMDFRNYMM RDENLEPLPK
310 320 330 340 350
NWEMAYTDTG TIYFIDHNTK TTTWLDPRLC KKAKAPEDCE DGELPYGWEK
360 370 380 390 400
IEDPQYGTYY VDHLNQKTQF ENPVEEAKRK KQIGQAETHS AKTDVERAHF
410 420 430 440 450
TRDPSQLKGV LVRASLKKST MGFGFTIIGG DRPDEFLQVK NVLKDGPAAQ
460 470 480 490 500
DGKMAPGDVI VDINGNCVLG HTHADVVQMF QLVPVNQYVN LTLCRGYALP
510 520 530 540 550
DDSEDPVVDI VAATPVINGQ SLAKGEACMS TQDFKLGAMV LDQNGKSGKL
560 570 580 590 600
LSSDRLNGPS DSNEQRASLA SSGSSQPELV TIPLVKGPKG FGFAIADSPT
610 620 630 640 650
GQKVKMILDS QWCQGLQKGD IIKEIYHQNV QNLTHLQVVE VLKQFPVGAD
660 670 680 690 700
VPLLILRGGP CSPTKTAKMK TDTKETSGSL ETINEPTPQP MPFPPSIIRS
710 720 730 740 750
GSPKLDPSEV YLKSKTLYED KPPNTKDLDV FLRKQESGFG FRVLGGDGPD
760 770 780 790 800
QSIYIGAIIP LGAAEKDGRL RAADELMCID GIPVKGKSHK QVLDLMTTAA
810 820 830 840 850
RNGHVLLTVR RKIFYGEKQP EDESPQAFSQ SGSPRLNRTE LPTRSAPQES
860 870 880 890 900
YDVILQRKEN EGFGFVILTS KSKPPPGVIP HKIGRVIDGS PADRCGRLKV
910 920 930 940 950
GDHISAVNGQ SIVDLSHDNI VQLIKDAGVT VTLTVVAEEE HHGPPSGTNS
960 970 980 990 1000
ARQSPALQHR PMGQAQATHI PGDRTALEGE VGKDVCSSYR HSWSDHKHLA
1010 1020 1030 1040 1050
QPDTAVISVV GSRHSQSLGC YPVELERGPR GFGFSLRGGK EYNMGLFILR
1060 1070 1080 1090 1100
LAEDGPAIKD GRIHVGDQIV EINGEPTQGI THTRAIELIQ AGGNKVLLLL
1110 1120 1130 1140 1150
RPGTGLIPDH GDWDIYSPSS SNVIYDEQPP PLPSSHSAAT FEESHVPVTE
1160 1170 1180 1190 1200
DSLIRVQTCE KAEELKDTVQ EKKSTLNGSQ PEMKYQSIQK NVSKKDPSRS
1210 1220 1230 1240 1250
HGHGDKNLLK GENGVTRRGR SASPKKSVNR HSEEHLEKIP RPLRSDPKGK
1260 1270 1280 1290 1300
SRDRSLSPRK GENKGQVTIK AGSGQDPCRK DRGRSSSPRK QQKIGGNSLS
1310 1320 1330 1340 1350
NTEGKLSEAG SRRAAGLSSD SPEQLPEGKE KSGVSRKDLK LSQLGKNRTR
1360 1370 1380 1390 1400
SPEKRSSKVD EASLPSKKTS DTASRVVSEK EKGRKPGTGE RSRDKTGESV
1410 1420 1430 1440 1450
QTSAKPLTQE AGEKMALSKA SEVTDRGKER AGGAPESSSP VKKAPITPGP
1460 1470
WRVPRANKVT GTAGMADKQL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1M7S0 | F1M7S0_RAT | Membrane-associated guanylate kinas... | Magi3 | 1,470 | Annotation score: | ||
A0A0G2JUX7 | A0A0G2JUX7_RAT | Membrane-associated guanylate kinas... | Magi3 | 1,179 | Annotation score: |
Sequence cautioni
The sequence AAF66069 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF255614 mRNA Translation: AAF66069.1 Frameshift. |
RefSeqi | NP_620784.2, NM_139084.2 |
Genome annotation databases
GeneIDi | 245903 |
KEGGi | rno:245903 |
UCSCi | RGD:621362, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF255614 mRNA Translation: AAF66069.1 Frameshift. |
RefSeqi | NP_620784.2, NM_139084.2 |
3D structure databases
SMRi | Q9JK71 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 251439, 3 interactors |
IntActi | Q9JK71, 2 interactors |
STRINGi | 10116.ENSRNOP00000026952 |
PTM databases
iPTMneti | Q9JK71 |
PhosphoSitePlusi | Q9JK71 |
Proteomic databases
PaxDbi | Q9JK71 |
PRIDEi | Q9JK71 |
Genome annotation databases
GeneIDi | 245903 |
KEGGi | rno:245903 |
UCSCi | RGD:621362, rat |
Organism-specific databases
CTDi | 260425 |
RGDi | 621362, Magi3 |
Phylogenomic databases
eggNOGi | KOG0707, Eukaryota KOG3209, Eukaryota |
InParanoidi | Q9JK71 |
OrthoDBi | 284488at2759 |
PhylomeDBi | Q9JK71 |
Miscellaneous databases
PROi | PR:Q9JK71 |
Family and domain databases
CDDi | cd00201, WW, 2 hits |
Gene3Di | 2.30.42.10, 6 hits |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR020590, Guanylate_kinase_CS IPR030035, MAGI3 IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR10316:SF10, PTHR10316:SF10, 1 hit |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 4 hits PF00397, WW, 2 hits |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 6 hits SM00456, WW, 2 hits |
SUPFAMi | SSF50156, SSF50156, 6 hits SSF51045, SSF51045, 2 hits SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00856, GUANYLATE_KINASE_1, 1 hit PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 6 hits PS01159, WW_DOMAIN_1, 2 hits PS50020, WW_DOMAIN_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAGI3_RAT | |
Accessioni | Q9JK71Primary (citable) accession number: Q9JK71 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | April 7, 2021 | |
This is version 127 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families